Gene/Proteome Database (LMPD)
LMPD ID
LMP010235
Gene ID
Species
Arabidopsis thaliana (Arabidopsis)
Gene Name
galactolipase DONGLE
Gene Symbol
Synonyms
DONGLE; T20M3.6
Alternate Names
galactolipase DONGLE
Chromosome
1
EC Number
3.1.1.26
Summary
Encodes a galactolipase. Located in the chloroplast. Involved in the initial step of jasmonic acid biosynthesis. Expressed in vegetative tissues and is necessary for the biosynthesis of basal-level JAs in vegetative tissues.
Orthologs
Proteins
| galactolipase DONGLE | |
|---|---|
| Refseq ID | NP_563748 |
| Protein GI | 18390571 |
| UniProt ID | Q9MA46 |
| mRNA ID | NM_100460 |
| Length | 471 |
| RefSeq Status | REVIEWED |
| MAAKVFTQNPIYSQSLVRDKTPQQKHNLDHFSISQHTSKRLVVSSSTMSPPISSSPLSLPSSSSSQAIPPSRAPAVTLPLSRVWREIQGSNNWENLIEPLSPILQQEITRYGNLLSASYKGFDLNPNSKRYLSCKYGKKNLLKESGIHDPDGYQVTKYIYATPDINLNPIKNEPNRARWIGYVAVSSDESVKRLGRRDILVTFRGTVTNHEWLANLKSSLTPARLDPHNPRPDVKVESGFLGLYTSGESESKFGLESCREQLLSEISRLMNKHKGEEISITLAGHSMGSSLAQLLAYDIAELGMNQRRDEKPVPVTVFSFAGPRVGNLGFKKRCEELGVKVLRITNVNDPITKLPGFLFNENFRSLGGVYELPWSCSCYTHVGVELTLDFFDVQNISCVHDLETYITLVNRPRCSKLAVNEDNFGGEFLNRTSELMFSKGRRQALHFTNAATNAAYLLCSISNHMLYYNIF | |
Gene Information
Gene Ontology (GO Annotations)
| GO ID | Source | Type | Description |
|---|---|---|---|
| GO:0009507 | IDA:TAIR | C | chloroplast |
| GO:0005811 | IDA:TAIR | C | lipid particle |
| GO:0052740 | IEA:UniProtKB-EC | F | 1-acyl-2-lysophosphatidylserine acylhydrolase activity |
| GO:0047714 | IDA:TAIR | F | galactolipase activity |
| GO:0008970 | IDA:TAIR | F | phosphatidylcholine 1-acylhydrolase activity |
| GO:0052739 | IEA:UniProtKB-EC | F | phosphatidylserine 1-acylhydrolase activity |
| GO:0050832 | IMP:TAIR | P | defense response to fungus |
| GO:0009695 | IMP:TAIR | P | jasmonic acid biosynthetic process |
| GO:0016042 | IEA:UniProtKB-KW | P | lipid catabolic process |
| GO:0030308 | IMP:TAIR | P | negative regulation of cell growth |
| GO:0009611 | IEP:TAIR | P | response to wounding |
BIOCYC Pathway Links
| BIOCYC Pathway ID | Description |
|---|---|
| PWY-6803 | phosphatidylcholine acyl editing |
| LIPASYN-PWY | phospholipases |
| LIPAS-PWY | triacylglycerol degradation |
Domain Information
UniProt Annotations
Entry Information
Comments
| Comment Type | Description |
|---|---|
| Biophysicochemical Properties | pH dependence: Optimum pH is 7.0. {ECO:0000269|PubMed:18267087}; |
| Catalytic Activity | 1,2-diacyl-3-beta-D-galactosyl-sn-glycerol + 2 H(2)O = 3-beta-D-galactosyl-sn-glycerol + 2 carboxylates. {ECO:0000269|PubMed:19527719}. |
| Catalytic Activity | Phosphatidylcholine + H(2)O = 2- acylglycerophosphocholine + a carboxylate. {ECO:0000269|PubMed:19527719}. |
| Disruption Phenotype | No visible phenotype under standard growth phenotype. {ECO:0000269|PubMed:20348210}. |
| Function | Sn-1-specific phospholipase that releases free fatty acids from phosphatidylcholine. Has a higher galactolipase activity than phospholipase A1 activity when digalactosyldiacylglycerol (DGDG) is used as substrate. Catalyzes the initial step of jasmonic acid biosynthesis. Required for the biosynthesis of basal-level endogenous jasmonate in vegetative tissues. Regulates leaves growth. Not essential for jasmonate biosynthesis after wounding or upon pathogen infection. {ECO:0000269|PubMed:18267087, ECO:0000269|PubMed:20348210}. |
| Induction | By wounding. {ECO:0000269|PubMed:18267087, ECO:0000269|PubMed:20348210}. |
| Miscellaneous | Overexpression of DGL causes a dwarf phenotype. |
| Similarity | Belongs to the AB hydrolase superfamily. Lipase family. {ECO:0000305}. |
| Subcellular Location | Plastid, chloroplast {ECO:0000269|PubMed:18267087, ECO:0000269|PubMed:19527719, ECO:0000269|PubMed:20348210}. Note=PubMed:20348210 shows a localization in lipid droplets. |
| Tissue Specificity | Expressed in leaves and seedlings. Not detected in flowers, siliques or roots. {ECO:0000269|PubMed:18267087, ECO:0000269|PubMed:19527719}. |
Identical and Related Proteins
Unique RefSeq proteins for LMP010235 (as displayed in Record Overview)
| Protein GI | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| 18390571 | RefSeq | NP_563748 | 471 | galactolipase DONGLE |
Identical Sequences to LMP010235 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:18390571 | EMBL | CBC12274.1 | 471 | unnamed protein product [Arabidopsis thaliana] |
| GI:18390571 | EMBL | CBD29177.1 | 471 | unnamed protein product [Arabidopsis thaliana] |
| GI:18390571 | EMBL | CBC90589.1 | 471 | unnamed protein product [Arabidopsis thaliana] |
| GI:18390571 | EMBL | CBC85662.1 | 471 | unnamed protein product [Arabidopsis thaliana] |
| GI:18390571 | GenBank | ADT59583.1 | 471 | Sequence 56 from patent US 7847156 |
| GI:18390571 | GenBank | AEE27895.1 | 471 | galactolipase DONGLE [Arabidopsis thaliana] |
Related Sequences to LMP010235 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:18390571 | GenBank | EFH65825.1 | 471 | lipase class 3 family protein [Arabidopsis lyrata subsp. lyrata] |
| GI:18390571 | GenBank | EOA37282.1 | 475 | hypothetical protein CARUB_v10010888mg [Capsella rubella] |
| GI:18390571 | RefSeq | XP_002889566.1 | 471 | lipase class 3 family protein [Arabidopsis lyrata subsp. lyrata] |
| GI:18390571 | RefSeq | XP_006304384.1 | 475 | hypothetical protein CARUB_v10010888mg [Capsella rubella] |
| GI:18390571 | RefSeq | XP_010457679.1 | 472 | PREDICTED: galactolipase DONGLE, chloroplastic [Camelina sativa] |
| GI:18390571 | RefSeq | XP_010475298.1 | 472 | PREDICTED: galactolipase DONGLE, chloroplastic-like [Camelina sativa] |