Gene/Proteome Database (LMPD)

LMPD ID
LMP010418
Gene ID
818664
Species
Arabidopsis thaliana (Arabidopsis)
Gene Name
glycerol-3-phosphate dehydrogenase [NAD(+)] 2
Gene Symbol
GLY1
Synonyms
SFD1; SUPPRESSOR OF FATTY ACID DESATURASE DEFICIENCY 1; T7D17.13; T7D17_13
Alternate Names
glycerol-3-phosphate dehydrogenase [NAD(+)] 2
Chromosome
2
EC Number
1.1.1.8
Summary
Encodes a putative dihydroxyacetone phosphate (DHAP) reductase involved in glycerol-3-phosphate supply within the chloroplast for synthesis of glycerolipids. Mutants have reduced levels of hexadecatrienoic acid, which is rescued by exogenous glycerol-3-phosphate. This gene appears to be involved in the flux of fatty acids in the prokaryotic glyerolipid biosynthesis pathway.
Orthologs
View orthologs and multiple alignments for GLY1

Proteins

glycerol-3-phosphate dehydrogenase [NAD(+)] 2
Refseq ID NP_565939
Protein GI 18405476
UniProt ID Q949Q0
mRNA ID NM_129631
Length 420
RefSeq Status REVIEWED
MAASVQPACLDLHFSGKHPPLLKHNAIIVRCVSSPNVIPEADSISGPPDIINTNRDQRKVVRIAWEKLVRWSRSLRAKAKTDVLERTRKVVVLGGGSFGTAMAAHVARRKEGLEVNMLVRDSFVCQSINENHHNCKYFPEHKLPENVIATTDAKAALLDADYCLHAVPVQFSSSFLEGIADYVDPGLPFISLSKGLELNTLRMMSQIIPIALKNPRQPFVALSGPSFALELMNNLPTAMVVASKDKKLANAVQQLLASSYLRINTSSDVTGVEIAGALKNVLAIAAGIVDGMNLGNNSMAALVSQGCSEIRWLATKMGAKPTTITGLSGTGDIMLTCFVNLSRNRTVGVRLGSGETLDDILTSMNQVAEGVATAGAVIALAQKYNVKLPVLTAVAKIIDNELTPTKAVLELMNLPQIEEV

Gene Information

Entrez Gene ID
818664
Gene Name
glycerol-3-phosphate dehydrogenase [NAD(+)] 2
Gene Symbol
GLY1
Species
Arabidopsis thaliana

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0009941 IDA:TAIR C chloroplast envelope
GO:0009331 IEA:InterPro C glycerol-3-phosphate dehydrogenase complex
GO:0004367 IGI:TAIR F glycerol-3-phosphate dehydrogenase [NAD+] activity
GO:0051287 IEA:InterPro F NAD binding
GO:0005975 IEA:InterPro P carbohydrate metabolic process
GO:0046168 IEA:InterPro P glycerol-3-phosphate catabolic process
GO:0045017 IMP:TAIR P glycerolipid biosynthetic process
GO:0046486 IMP:TAIR P glycerolipid metabolic process
GO:0006650 IEA:UniProtKB-UniPathway P glycerophospholipid metabolic process
GO:0008654 IEA:UniProtKB-KW P phospholipid biosynthetic process
GO:0009627 IMP:TAIR P systemic acquired resistance

REACTOME Pathway Links

REACTOME Pathway ID Description
6253801 Glycerophospholipid biosynthesis
6253802 Phospholipid metabolism
6253800 Synthesis of PA
6253791 Triglyceride Biosynthesis

Domain Information

InterPro Annotations

Accession Description
IPR008927 6-phosphogluconate dehydrogenase, C-terminal-like
IPR013328 Dehydrogenase, multihelical
IPR006168 Glycerol-3-phosphate dehydrogenase, NAD-dependent
IPR006109 Glycerol-3-phosphate dehydrogenase, NAD-dependent, C-terminal
IPR011128 Glycerol-3-phosphate dehydrogenase, NAD-dependent, N-terminal
IPR016040 NAD(P)-binding domain

UniProt Annotations

Entry Information

Gene Name
glycerol-3-phosphate dehydrogenase [NAD(+)] 2
Protein Entry
GPDA2_ARATH
UniProt ID
Q949Q0
Species
Arabidopsis

Comments

Comment Type Description
Biophysicochemical Properties Kinetic parameters: KM=0.33 uM for glycerone phosphate {ECO:0000269|PubMed:22645576}; Vmax=49 umol/min/mg enzyme toward glycerone phosphate {ECO:0000269|PubMed:22645576};
Catalytic Activity sn-glycerol 3-phosphate + NAD(+) = glycerone phosphate + NADH. {ECO:0000269|PubMed:14729910, ECO:0000269|PubMed:15044700, ECO:0000269|PubMed:22645576}.
Disruption Phenotype No visible phenotype under normal growth conditions, but leaves have decreased levels of hexadecatrienoic fatty acid (16:3) and increased levels of oleic acid (18:1). {ECO:0000269|PubMed:14729910, ECO:0000269|PubMed:15044700}.
Function Required to supply glycerol-3-phosphate in the chloroplast for the synthesis of glycerolipids. Required for activation of systemic acquired resistance (SAR). Provision of glycerol-3-phosphate may be involved in generating lipid signals necessary for mediating defense responses and SAR. {ECO:0000269|PubMed:14729910, ECO:0000269|PubMed:15044700, ECO:0000269|PubMed:17431038, ECO:0000269|PubMed:18088304, ECO:0000269|PubMed:22645576}.
Pathway Membrane lipid metabolism; glycerophospholipid metabolism.
Similarity Belongs to the NAD-dependent glycerol-3-phosphate dehydrogenase family. {ECO:0000305}.
Subcellular Location Plastid, chloroplast {ECO:0000250}.

Identical and Related Proteins

Unique RefSeq proteins for LMP010418 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
18405476 RefSeq NP_565939 420 glycerol-3-phosphate dehydrogenase [NAD(+)] 2

Identical Sequences to LMP010418 proteins

Reference Database Accession Length Protein Name
GI:18405476 GenBank AAK93645.1 420 putative glycerol-3-phosphate dehydrogenase [Arabidopsis thaliana]
GI:18405476 GenBank AAM45126.1 420 putative glycerol-3-phosphate dehydrogenase [Arabidopsis thaliana]
GI:18405476 GenBank AEC09864.1 420 glycerol-3-phosphate dehydrogenase [NAD(+)] 2 [Arabidopsis thaliana]
GI:18405476 SwissProt Q949Q0.1 420 RecName: Full=Glycerol-3-phosphate dehydrogenase [NAD(+)] 2, chloroplastic; AltName: Full=Protein SUPPRESSOR OF FATTY ACID DESATURASE DEFICIENCY 1; Flags: Precursor [Arabidopsis thaliana]

Related Sequences to LMP010418 proteins

Reference Database Accession Length Protein Name
GI:18405476 GenBank EFH56144.1 420 hypothetical protein ARALYDRAFT_483127 [Arabidopsis lyrata subsp. lyrata]
GI:18405476 GenBank EOA27106.1 449 hypothetical protein CARUB_v10023204mg, partial [Capsella rubella]
GI:18405476 RefSeq XP_002879885.1 420 hypothetical protein ARALYDRAFT_483127 [Arabidopsis lyrata subsp. lyrata]
GI:18405476 RefSeq XP_006294208.1 449 hypothetical protein CARUB_v10023204mg, partial [Capsella rubella]
GI:18405476 RefSeq XP_010505823.1 420 PREDICTED: glycerol-3-phosphate dehydrogenase [NAD(+)] 2, chloroplastic-like [Camelina sativa]
GI:18405476 RefSeq XP_010508819.1 420 PREDICTED: glycerol-3-phosphate dehydrogenase [NAD(+)] 2, chloroplastic [Camelina sativa]