Gene/Proteome Database (LMPD)

LMPD ID
LMP010448
Gene ID
841247
Species
Arabidopsis thaliana (Arabidopsis)
Gene Name
G-protein-coupled receptor 1
Gene Symbol
GCR1
Synonyms
F11A17.17; F11A17_17; G-protein-coupled receptor 1; GCR1
Alternate Names
G-protein-coupled receptor 1
Chromosome
1
Summary
encodes a protein similar to G-coupled receptor with 7 transmembrane regions. Overexpression studies suggest this gene is involved in dormancy and flowering. Reduction of expression results in decreased sensitivity to cytokinin.
Orthologs
View orthologs and multiple alignments for GCR1

Proteins

G-protein-coupled receptor 1
Refseq ID NP_175261
Protein GI 15221138
UniProt ID O04714
mRNA ID NM_103724
Length 326
RefSeq Status REVIEWED
MSAVLTAGGGLTAGDRSIITAINTGASSLSFVGSAFIVLCYCLFKELRKFSFKLVFYLALSDMLCSFFLIVGDPSKGFICYAQGYTTHFFCVASFLWTTTIAFTLHRTVVKHKTDVEDLEAMFHLYVWGTSLVVTVIRSFGNNHSHLGPWCWTQTGLKGKAVHFLTFYAPLWGAILYNGFTYFQVIRMLRNARRMAVGMSDRVDQFDNRAELKVLNRWGYYPLILIGSWAFGTINRIHDFIEPGHKIFWLSVLDVGTAALMGLFNSIAYGFNSSVRRAIHERLELFLPERLYRWLPSNFRPKNHLILHNQRSEMVSLKTEDQQ

Gene Information

Entrez Gene ID
841247
Gene Name
G-protein-coupled receptor 1
Gene Symbol
GCR1
Species
Arabidopsis thaliana

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005794 IDA:TAIR C Golgi apparatus
GO:0044214 NAS:UniProtKB C fully spanning plasma membrane
GO:0016021 IEA:UniProtKB-KW C integral component of membrane
GO:0005886 IDA:UniProtKB C plasma membrane
GO:0005774 IDA:TAIR C vacuolar membrane
GO:0004930 ISS:TAIR F G-protein coupled receptor activity
GO:0009094 IMP:TAIR P L-phenylalanine biosynthetic process
GO:0009738 IMP:UniProtKB P abscisic acid-activated signaling pathway
GO:0007202 IMP:UniProtKB P activation of phospholipase C activity
GO:0009785 IMP:UniProtKB P blue light signaling pathway
GO:0009742 IMP:UniProtKB P brassinosteroid mediated signaling pathway
GO:0009736 IEA:UniProtKB-KW P cytokinin-activated signaling pathway
GO:0009908 IMP:TAIR P flower development
GO:0009740 IEA:UniProtKB-KW P gibberellic acid mediated signaling pathway
GO:0006629 IEA:UniProtKB-KW P lipid metabolic process
GO:0010231 IMP:TAIR P maintenance of seed dormancy
GO:0000278 IDA:TAIR P mitotic cell cycle
GO:0009788 IMP:UniProtKB P negative regulation of abscisic acid-activated signaling pathway
GO:0009939 IMP:UniProtKB P positive regulation of gibberellic acid mediated signaling pathway
GO:0032960 IMP:UniProtKB P regulation of inositol trisphosphate biosynthetic process
GO:0009735 IMP:TAIR P response to cytokinin
GO:0010244 IMP:TAIR P response to low fluence blue light stimulus by blue low-fluence system
GO:0009845 IEP:UniProtKB P seed germination
GO:0006571 IMP:TAIR P tyrosine biosynthetic process

Domain Information

InterPro Annotations

Accession Description
IPR022340 G protein-coupled receptor GCR1 putative
IPR022343 GCR1-cAMP receptor
IPR017981 GPCR, family 2-like
IPR017452 GPCR, rhodopsin-like, 7TM

UniProt Annotations

Entry Information

Gene Name
G-protein-coupled receptor 1
Protein Entry
GCR1_ARATH
UniProt ID
O04714
Species
Arabidopsis

Comments

Comment Type Description
Developmental Stage In seedlings, mostly expressed in small roots, and to a lower extent in hypocotyls. In young plants, equaly expressed in leaves, roots, and shoot tip. In old plants, present in roots, flower buds and young siliques, but not in leaves. {ECO:0000269|PubMed:11930019}.
Disruption Phenotype Hypersensitivity to abscisic acid (ABA) and to the lipid metabolite, sphingosine-1-phosphate (S1P), a transducer of the ABA signal. Improved drought tolerance accompanied by lower rates of water loss. Impaired sensitivity to gibberellin (GA) and brassinosteroid (BR) in seed germination. Hypersensitivity to ABA and glucose (Glc) during and after seed germination. Altered response to blue light (BL). {ECO:0000269|PubMed:15155892, ECO:0000269|PubMed:15181210, ECO:0000269|PubMed:16415218, ECO:0000269|PubMed:16581874, ECO:0000269|PubMed:17322342}.
Function Together with GPA1, may regulate the cell cycle via a signaling cascade that uses phosphatidylinositol-specific phospholipase C (PI-PLC) as an effector and inositol 1,4,5- trisphosphate(IP(3)) as a second messenger. Acts as a negative regulator of GPA1-mediated abscisic acid (ABA) responses in guard cells, and together with GPA1 and GB1 during seed germination and early seedling development. Promotes PI-PLC activity and IP(3) accumulation. Involved in the blue light (BL) signaling. Together with GPA1 and ADT3, required for BL-mediated synthesis of phenylpyruvate and subsequently of phenylalanine (Phe), in etiolated seedlings. Probably involved in cytokinin signal transduction. Plays a positive role in gibberellin- (GA) and brassinosteroid- (BR) regulated seed germination, probably independently of a heterotrimeric G-protein. Mediates seed dormancy abolition, and promotes seed germination and flowering. {ECO:0000269|PubMed:11930019, ECO:0000269|PubMed:12972659, ECO:0000269|PubMed:15155892, ECO:0000269|PubMed:15181210, ECO:0000269|PubMed:16415218, ECO:0000269|PubMed:16581874, ECO:0000269|PubMed:17322342, ECO:0000269|PubMed:9512416}.
Induction Modulated during the cell cycle with a peak during the early G(1) phase. Induced upon imbibition. {ECO:0000269|PubMed:11930019, ECO:0000269|PubMed:16581874}.
Interaction P18064:GPA1; NbExp=6; IntAct=EBI-443899, EBI-443890;
Similarity Belongs to the G-protein coupled receptor 2 family. {ECO:0000305}.
Subcellular Location Cell membrane {ECO:0000269|PubMed:15181210, ECO:0000269|Ref.7}; Multi-pass membrane protein {ECO:0000269|PubMed:15181210, ECO:0000269|Ref.7}. Note=Localized to the outer edge of the leaf epidermal cells in a punctuate pattern.
Subunit Interacts with GPA1. {ECO:0000269|PubMed:15155892}.
Tissue Specificity Mostly present in the meristematic regions. Expressed at low levels in seedlings, vascular tissues of cotyledons, hypocotyl, and roots, stems, leaves, flowering buds and siliques. In dark-grown seedlings, localized in the cotyledons and the hook. {ECO:0000269|PubMed:11930019, ECO:0000269|PubMed:15181210, ECO:0000269|PubMed:9512416}.

Identical and Related Proteins

Unique RefSeq proteins for LMP010448 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
15221138 RefSeq NP_175261 326 G-protein-coupled receptor 1

Identical Sequences to LMP010448 proteins

Reference Database Accession Length Protein Name
GI:15221138 DBBJ BAF00405.1 326 putative G-protein-coupled receptor [Arabidopsis thaliana]
GI:15221138 GenBank AAC49962.1 326 putative G-protein-coupled receptor [Arabidopsis thaliana]
GI:15221138 GenBank AAD49769.1 326 Identical to a putative G-protein-coupled receptor from Arabidopsis thaliana gb|U95143. EST gb|T04329 comes from this gene [Arabidopsis thaliana]
GI:15221138 GenBank AEE32270.1 326 G-protein-coupled receptor 1 [Arabidopsis thaliana]
GI:15221138 GenBank AGD03375.1 326 Sequence 831 from patent US 8343764
GI:15221138 SwissProt O04714.1 326 RecName: Full=G-protein coupled receptor 1 [Arabidopsis thaliana]

Related Sequences to LMP010448 proteins

Reference Database Accession Length Protein Name
GI:15221138 EMBL CAA72145.1 326 putative G protein coupled receptor [Arabidopsis thaliana]
GI:15221138 GenBank AAM20722.1 326 putative G protein-coupled receptor [Arabidopsis thaliana]
GI:15221138 GenBank AAN15633.1 326 putative G protein-coupled receptor [Arabidopsis thaliana]
GI:15221138 GenBank AGD17503.1 326 Sequence 14959 from patent US 8343764
GI:15221138 GenBank AGD19168.1 326 Sequence 16624 from patent US 8343764
GI:15221138 RefSeq XP_010479247.1 326 PREDICTED: G-protein coupled receptor 1 [Camelina sativa]