Gene/Proteome Database (LMPD)
LMPD ID
LMP010487
Gene ID
Species
Arabidopsis thaliana (Arabidopsis)
Gene Name
homogentisate phytyltransferase 1
Gene Symbol
Synonyms
ATHPT; F19F24.15; F19F24_15; HOMOGENTISATE PHYTYLTRANSFERASE; homogentisate phytyltransferase 1; HPT1; TOCOPHEROL PHYTYLTRANSFERASE; TOCOPHEROL POLYPRENYLTRANSFERASE; TPT1; VITAMIN E 2; VTE2
Alternate Names
homogentisate phytyltransferase 1
Chromosome
2
EC Number
2.5.1.115
Summary
Encodes homogentisate phytyltransferase involved in tocopherol biosynthesis. Has impact on seed longevity and plays a role in the adaptation to low temperature stress, notably phloem loading.
Orthologs
Proteins
| homogentisate phytyltransferase 1 | |
|---|---|
| Refseq ID | NP_849984 |
| Protein GI | 30680535 |
| UniProt ID | Q8VWJ1 |
| mRNA ID | NM_179653 |
| Length | 393 |
| RefSeq Status | REVIEWED |
| MESLLSSSSLVSAAGGFCWKKQNLKLHSLSEIRVLRCDSSKVVAKPKFRNNLVRPDGQGSSLLLYPKHKSRFRVNATAGQPEAFDSNSKQKSFRDSLDAFYRFSRPHTVIGTVLSILSVSFLAVEKVSDISPLLFTGILEAVVAALMMNIYIVGLNQLSDVEIDKVNKPYLPLASGEYSVNTGIAIVASFSIMSFWLGWIVGSWPLFWALFVSFMLGTAYSINLPLLRWKRFALVAAMCILAVRAIIVQIAFYLHIQTHVFGRPILFTRPLIFATAFMSFFSVVIALFKDIPDIEGDKIFGIRSFSVTLGQKRVFWTCVTLLQMAYAVAILVGATSPFIWSKVISVVGHVILATTLWARAKSVDLSSKTEITSCYMFIWKLFYAEYLLLPFLK | |
Gene Information
Entrez Gene ID
Gene Name
homogentisate phytyltransferase 1
Gene Symbol
Species
Arabidopsis thaliana
Gene Ontology (GO Annotations)
| GO ID | Source | Type | Description |
|---|---|---|---|
| GO:0009507 | ISS:TAIR | C | chloroplast |
| GO:0016021 | IEA:UniProtKB-KW | C | integral component of membrane |
| GO:0009579 | IEA:UniProtKB-KW | C | thylakoid |
| GO:0010176 | IDA:TAIR | F | homogentisate phytyltransferase activity |
| GO:0071555 | IMP:TAIR | P | cell wall organization |
| GO:0009915 | IMP:TAIR | P | phloem sucrose loading |
| GO:0031347 | IMP:TAIR | P | regulation of defense response |
| GO:0009266 | IMP:TAIR | P | response to temperature stimulus |
| GO:0006636 | IMP:TAIR | P | unsaturated fatty acid biosynthetic process |
| GO:0010189 | IMP:TAIR | P | vitamin E biosynthetic process |
KEGG Pathway Links
| KEGG Pathway ID | Description |
|---|---|
| ath01110 | Biosynthesis of secondary metabolites |
| ath01100 | Metabolic pathways |
| ath_M00112 | Tocopherol biosynthesis |
| ath00130 | Ubiquinone and other terpenoid-quinone biosynthesis |
Domain Information
InterPro Annotations
| Accession | Description |
|---|---|
| IPR000537 | UbiA prenyltransferase family |
UniProt Annotations
Entry Information
Gene Name
homogentisate phytyltransferase 1
Protein Entry
HPT1_ARATH
UniProt ID
Species
Arabidopsis
Comments
| Comment Type | Description |
|---|---|
| Catalytic Activity | Phytyl diphosphate + homogentisate = diphosphate + 2-methyl-6-phytylbenzene-1,4-diol + CO(2). {ECO:0000269|PubMed:16989822}. |
| Disruption Phenotype | Reduced seed longevity, severe seedling growth defects during germination and high levels of lipid hydroperoxides and hydroxy fatty acids. {ECO:0000269|PubMed:12586887}. |
| Function | Involved in the synthesis of tocopherol (vitamin E). Catalyzes the condensation of homogentisate and phytyl diphosphate to form dimethylphytylhydrquinone. Low activity with geranylgeranyl diphosphate as substrate, but no activity with farnesyl diphosphate or solanesyl diphosphate. Tocopherol functions to limit lipid oxidation during seed desiccation, quiescence and germination and early seedling development. Protects thylakoid membrane lipids from photooxidation and is required for low-temperature adaptation. {ECO:0000269|PubMed:11706191, ECO:0000269|PubMed:12011362, ECO:0000269|PubMed:12586887, ECO:0000269|PubMed:16258032, ECO:0000269|PubMed:16989822, ECO:0000269|PubMed:17012603, ECO:0000269|PubMed:18314499, ECO:0000269|PubMed:20691023, ECO:0000269|PubMed:20837525, ECO:0000269|PubMed:21223386}. |
| Miscellaneous | Seeds and plants overexpressing HPT1 accumulate increased levels of tocopherol. |
| Pathway | Cofactor biosynthesis; tocopherol biosynthesis. |
| Sequence Caution | Sequence=AAC09029.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305}; |
| Similarity | Belongs to the UbiA prenyltransferase family. {ECO:0000305}. |
| Subcellular Location | Plastid, chloroplast membrane {ECO:0000269|PubMed:21223386}; Multi-pass membrane protein {ECO:0000255}. |
Identical and Related Proteins
Unique RefSeq proteins for LMP010487 (as displayed in Record Overview)
| Protein GI | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| 30680535 | RefSeq | NP_849984 | 393 | homogentisate phytyltransferase 1 |
Identical Sequences to LMP010487 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:30680535 | GenBank | AFU23535.1 | 393 | Sequence 13 from patent US 8269076 |
| GI:30680535 | GenBank | AFX28264.1 | 393 | Sequence 9324 from patent US 8299318 |
| GI:30680535 | GenBank | AFX48896.1 | 393 | Sequence 50729 from patent US 8299318 |
| GI:30680535 | GenBank | AGF06978.1 | 393 | Sequence 2 from patent US 8362324 |
| GI:30680535 | GenBank | AGF15337.1 | 393 | Sequence 14756 from patent US 8362325 |
| GI:30680535 | GenBank | AGP18095.1 | 393 | Sequence 13 from patent US 8461427 |
Related Sequences to LMP010487 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:30680535 | EMBL | CAC18909.1 | 393 | unnamed protein product [Arabidopsis thaliana] |
| GI:30680535 | GenBank | AAW03945.1 | 393 | Sequence 2 from patent US 6787683 |
| GI:30680535 | GenBank | ACJ54263.1 | 393 | homogentisate phytyltransferase 1 [Binary vector pCAMBIA1300-VE2-7S] |
| GI:30680535 | GenBank | ACJ54276.1 | 393 | homogentisate phytyltransferase 1 [Binary vector pCAMBIA1300-VE2-VE3] |
| GI:30680535 | GenBank | EOA30750.1 | 394 | hypothetical protein CARUB_v10013893mg [Capsella rubella] |
| GI:30680535 | RefSeq | XP_006297852.1 | 394 | hypothetical protein CARUB_v10013893mg [Capsella rubella] |