Gene/Proteome Database (LMPD)

LMPD ID
LMP010492
Gene ID
826788
Species
Arabidopsis thaliana (Arabidopsis)
Gene Name
Hydroxymethylglutaryl-CoA synthase
Gene Symbol
MVA1
Synonyms
EMB2778; EMBRYO DEFECTIVE 2778; FKP1; FLAKY POLLEN 1; HMG-COA SYNTHASE; HMGS; HYDROXYMETHYLGLUTARYL-COA SYNTHASE; MVA1; T26M18.30; T26M18_30
Alternate Names
Hydroxymethylglutaryl-CoA synthase
Chromosome
4
EC Number
2.3.3.10
Summary
Encodes a protein with hydroxymethylglutaryl-CoA synthase activity which was characterized by phenotypical complementation of the S. cerevisiae mutant.
Orthologs
View orthologs and multiple alignments for MVA1

Proteins

Hydroxymethylglutaryl-CoA synthase
Refseq ID NP_192919
Protein GI 15234313
UniProt ID P54873
mRNA ID NM_117251
Length 461
RefSeq Status REVIEWED
MAKNVGILAMDIYFPPTCVQQEALEAHDGASKGKYTIGLGQDCLAFCTELEDVISMSFNAVTSLFEKYKIDPNQIGRLEVGSETVIDKSKSIKTFLMQLFEKCGNTDVEGVDSTNACYGGTAALLNCVNWVESNSWDGRYGLVICTDSAVYAEGPARPTGGAAAIAMLIGPDAPIVFESKLRASHMAHVYDFYKPNLASEYPVVDGKLSQTCYLMALDSCYKHLCNKFEKIEGKEFSINDADYIVFHSPYNKLVQKSFARLLYNDFLRNASSIDEAAKEKFTPYSSLTLDESYQSRDLEKVSQQIAKPFYDAKVQPTTLIPKEVGNMYTASLYAAFASLIHKKHNDLAGKRVVMFSYGSGSTATMFSLRLNDNKPPFSISNIASVMDVGGKLKARHEYAPEKFVETMKLMEHRYGAKDFVTTKEGIIDLLAPGTYYLKEVDSLYRRFYGKKGEDGSVANGH
Hydroxymethylglutaryl-CoA synthase
Refseq ID NP_849361
Protein GI 30681902
UniProt ID P54873
mRNA ID NM_179030
Length 406
RefSeq Status REVIEWED
MSFNAVTSLFEKYKIDPNQIGRLEVGSETVIDKSKSIKTFLMQLFEKCGNTDVEGVDSTNACYGGTAALLNCVNWVESNSWDGRYGLVICTDSAVYAEGPARPTGGAAAIAMLIGPDAPIVFESKLRASHMAHVYDFYKPNLASEYPVVDGKLSQTCYLMALDSCYKHLCNKFEKIEGKEFSINDADYIVFHSPYNKLVQKSFARLLYNDFLRNASSIDEAAKEKFTPYSSLTLDESYQSRDLEKVSQQIAKPFYDAKVQPTTLIPKEVGNMYTASLYAAFASLIHKKHNDLAGKRVVMFSYGSGSTATMFSLRLNDNKPPFSISNIASVMDVGGKLKARHEYAPEKFVETMKLMEHRYGAKDFVTTKEGIIDLLAPGTYYLKEVDSLYRRFYGKKGEDGSVANGH

Gene Information

Entrez Gene ID
826788
Gene Name
Hydroxymethylglutaryl-CoA synthase
Gene Symbol
MVA1
Species
Arabidopsis thaliana

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005829 IDA:TAIR C cytosol
GO:0009506 IDA:TAIR C plasmodesma
GO:0004421 IGI:TAIR F hydroxymethylglutaryl-CoA synthase activity
GO:0019287 TAS:TAIR P isopentenyl diphosphate biosynthetic process, mevalonate pathway
GO:0016126 IEA:UniProtKB-KW P sterol biosynthetic process

KEGG Pathway Links

KEGG Pathway ID Description
ath_M00095 C5 isoprenoid biosynthesis, mevalonate pathway
ath00900 Terpenoid backbone biosynthesis

REACTOME Pathway Links

REACTOME Pathway ID Description
6254329 Activation of gene expression by SREBF (SREBP)
6254036 Cholesterol biosynthesis

Domain Information

InterPro Annotations

Accession Description
IPR010122 Hydroxymethylglutaryl-CoA synthase, eukaryotic
IPR013746 Hydroxymethylglutaryl-coenzyme A synthase C-terminal domain
IPR013528 Hydroxymethylglutaryl-coenzyme A synthase, N-terminal
IPR000590 Hydroxymethylglutaryl-coenzyme A synthase, active site
IPR016039 Thiolase-like
IPR016038 Thiolase-like, subgroup

UniProt Annotations

Entry Information

Gene Name
Hydroxymethylglutaryl-CoA synthase
Protein Entry
HMCS_ARATH
UniProt ID
P54873
Species
Arabidopsis

Comments

Comment Type Description
Alternative Products Event=Alternative splicing; Named isoforms=2; Name=1; IsoId=P54873-1; Sequence=Displayed; Name=2; IsoId=P54873-2; Sequence=VSP_008902;
Catalytic Activity Acetyl-CoA + H(2)O + acetoacetyl-CoA = (S)-3- hydroxy-3-methylglutaryl-CoA + CoA. {ECO:0000255|PROSITE- ProRule:PRU10116, ECO:0000269|PubMed:8566777}.
Disruption Phenotype Defect in pollen adhesion to the stigma resulting in male sterility under normal growth conditions. {ECO:0000269|PubMed:20484369}.
Function This enzyme condenses acetyl-CoA with acetoacetyl-CoA to form HMG-CoA, which is the substrate for HMG-CoA reductase. Devoided of acetoacetyl-CoA thiolase (AACT) activity. Required for the development of both tapetosomes and elaioplasts in tapetal cells and for pollen viability during pollen tube elongation. {ECO:0000269|PubMed:20484369}.
Pathway Metabolic intermediate biosynthesis; (R)-mevalonate biosynthesis; (R)-mevalonate from acetyl-CoA: step 2/3.
Similarity Belongs to the HMG-CoA synthase family. {ECO:0000305}.
Tissue Specificity Highly expressed in flower buds, pollen grains and anthers in the tapetal cells, at an intermediate levels in roots and weakly in leaves. {ECO:0000269|PubMed:20484369}.

Identical and Related Proteins

Unique RefSeq proteins for LMP010492 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
15234313 RefSeq NP_192919 461 Hydroxymethylglutaryl-CoA synthase
30681902 RefSeq NP_849361 406 Hydroxymethylglutaryl-CoA synthase

Identical Sequences to LMP010492 proteins

Reference Database Accession Length Protein Name
GI:15234313 EMBL CAB44320.1 461 hydroxymethylglutaryl-CoA synthase [Arabidopsis thaliana]
GI:15234313 EMBL CAB78225.1 461 hydroxymethylglutaryl-CoA synthase [Arabidopsis thaliana]
GI:15234313 GenBank AAD00297.1 461 HMG-CoA synthase [Arabidopsis thaliana]
GI:15234313 GenBank AAD00298.1 461 HMG-CoA synthase [Arabidopsis thaliana]
GI:30681902 GenBank AAM98150.1 406 hydroxymethylglutaryl-CoA synthase [Arabidopsis thaliana]
GI:30681902 GenBank AAP37851.1 406 At4g11820 [Arabidopsis thaliana]
GI:30681902 GenBank AEE83052.1 406 Hydroxymethylglutaryl-CoA synthase [Arabidopsis thaliana]
GI:15234313 GenBank AEE83053.1 461 Hydroxymethylglutaryl-CoA synthase [Arabidopsis thaliana]
GI:15234313 SwissProt P54873.2 461 RecName: Full=Hydroxymethylglutaryl-CoA synthase; Short=HMG-CoA synthase; AltName: Full=3-hydroxy-3-methylglutaryl coenzyme A synthase; AltName: Full=Protein EMBRYO DEFECTIVE 2778; AltName: Full=Protein FLAKY POLLEN 1 [Arabidopsis thaliana]

Related Sequences to LMP010492 proteins

Reference Database Accession Length Protein Name
GI:15234313 EMBL CAA58763.1 461 hydroxymethylglutaryl-CoA synthase [Arabidopsis thaliana]
GI:30681902 EMBL CAB44320.1 461 hydroxymethylglutaryl-CoA synthase [Arabidopsis thaliana]
GI:30681902 EMBL CAB78225.1 461 hydroxymethylglutaryl-CoA synthase [Arabidopsis thaliana]
GI:30681902 GenBank AAD00297.1 461 HMG-CoA synthase [Arabidopsis thaliana]
GI:30681902 GenBank AAD00298.1 461 HMG-CoA synthase [Arabidopsis thaliana]
GI:15234313 GenBank ABA16969.1 461 Sequence 35 from patent US 6916972
GI:15234313 GenBank ABL51217.1 461 Sequence 35 from patent US 7135622
GI:15234313 GenBank ABT05616.1 461 Sequence 35 from patent US 7217863
GI:15234313 GenBank EFH50985.1 461 hydroxymethylglutaryl-CoA synthase [Arabidopsis lyrata subsp. lyrata]
GI:30681902 GenBank AEE83053.1 461 Hydroxymethylglutaryl-CoA synthase [Arabidopsis thaliana]
GI:15234313 PRF - 461 hydroxy methylglutaryl CoA synthase [Arabidopsis thaliana]
GI:30681902 SwissProt P54873.2 461 RecName: Full=Hydroxymethylglutaryl-CoA synthase; Short=HMG-CoA synthase; AltName: Full=3-hydroxy-3-methylglutaryl coenzyme A synthase; AltName: Full=Protein EMBRYO DEFECTIVE 2778; AltName: Full=Protein FLAKY POLLEN 1 [Arabidopsis thaliana]