Gene/Proteome Database (LMPD)
LMPD ID
LMP010561
Gene ID
Species
Arabidopsis thaliana (Arabidopsis)
Gene Name
long chain acyl-CoA synthetase 1
Gene Symbol
Synonyms
CER8; ECERIFERUM 8; LACS1; LONG-CHAIN ACYL-COA SYNTHASE 1; T8I13.8
Alternate Names
long chain acyl-CoA synthetase 1
Chromosome
2
EC Number
6.2.1.3
Summary
Encodes an acyl-CoA synthetase that acts on long-chain and very-long-chain fatty acids, involved in cuticular wax and cutin biosynthesis
Orthologs
Proteins
| long chain acyl-CoA synthetase 1 | |
|---|---|
| Refseq ID | NP_001031554 |
| Protein GI | 79324939 |
| UniProt ID | O22898 |
| mRNA ID | NM_001036477 |
| Length | 660 |
| RefSeq Status | REVIEWED |
| MKSFAAKVEEGVKGIDGKPSVGPVYRNLLSEKGFPPIDSEITTAWDIFSKSVEKFPDNNMLGWRRIVDEKVGPYMWKTYKEVYEEVLQIGSALRAAGAEPGSRVGIYGVNCPQWIIAMEACAAHTLICVPLYDTLGSGAVDYIVEHAEIDFVFVQDTKIKGLLEPDCKCAKRLKAIVSFTNVSDELSHKASEIGVKTYSWIDFLHMGREKPEDTNPPKAFNICTIMYTSGTSGDPKGVVLTHQAVATFVVGMDLYMDQFEDKMTHDDVYLSFLPLAHILDRMNEEYFFRKGASVGYYHGNLNVLRDDIQELKPTYLAGVPRVFERIHEGIQKALQELNPRRRFIFNALYKHKLAWLNRGYSHSKASPMADFIAFRKIRDKLGGRIRLLVSGGAPLSPEIEEFLRVTCCCFVVQGYGLTETLGGTALGFPDEMCMLGTVGIPAVYNEIRLEEVSEMGYDPLGENPAGEICIRGQCMFSGYYKNPELTEEVMKDGWFHTGDIGEILPNGVLKIIDRKKNLIKLSQGEYVALEHLENIFGQNSVVQDIWVYGDSFKSMLVAVVVPNPETVNRWAKDLGFTKPFEELCSFPELKEHIISELKSTAEKNKLRKFEYIKAVTVETKPFDVERDLVTATLKNRRNNLLKYYQVQIDEMYRKLASKKI | |
Gene Information
Entrez Gene ID
Gene Name
long chain acyl-CoA synthetase 1
Gene Symbol
Species
Arabidopsis thaliana
Gene Ontology (GO Annotations)
| GO ID | Source | Type | Description |
|---|---|---|---|
| GO:0005783 | IDA:UniProtKB | C | endoplasmic reticulum |
| GO:0005524 | IEA:UniProtKB-KW | F | ATP binding |
| GO:0004467 | IDA:UniProtKB | F | long-chain fatty acid-CoA ligase activity |
| GO:0031957 | IDA:TAIR | F | very long-chain fatty acid-CoA ligase activity |
| GO:0010143 | IMP:TAIR | P | cutin biosynthetic process |
| GO:0006631 | TAS:UniProtKB | P | fatty acid metabolic process |
| GO:0001676 | IDA:GOC | P | long-chain fatty acid metabolic process |
| GO:0010025 | IMP:TAIR | P | wax biosynthetic process |
KEGG Pathway Links
| KEGG Pathway ID | Description |
|---|---|
| ath_M00086 | beta-Oxidation, acyl-CoA synthesis |
| ath00061 | Fatty acid biosynthesis |
| ath00071 | Fatty acid degradation |
| ath04146 | Peroxisome |
Domain Information
UniProt Annotations
Entry Information
Gene Name
long chain acyl-CoA synthetase 1
Protein Entry
LACS1_ARATH
UniProt ID
Species
Arabidopsis
Comments
| Comment Type | Description |
|---|---|
| Catalytic Activity | ATP + a long-chain fatty acid + CoA = AMP + diphosphate + an acyl-CoA. {ECO:0000269|PubMed:12177484}. |
| Cofactor | Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250}; |
| Disruption Phenotype | In stem, fewer and flatter wax crystals and disorganized cuticle proper and thinner cuticular layer. Reduced amount of wax in all chemical classes on the stem and leaf, except in the very long-chain fatty acid (VLCFA) class with acids longer than 24 carbons (C(24)). {ECO:0000269|PubMed:19392700, ECO:0000269|PubMed:20237894}. |
| Function | Activation of long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. Acts in both the wax and cutin pathways. Preferentially uses palmitate, palmitoleate, linoleate and eicosenoate. Seems to have a specific activity against very long-chain fatty acid (VLCFA) class with acids longer than 24 carbons (C(24)). {ECO:0000269|PubMed:12228482, ECO:0000269|PubMed:19392700, ECO:0000269|PubMed:20237894}. |
| Pathway | Lipid metabolism; fatty acid metabolism. |
| Similarity | Belongs to the ATP-dependent AMP-binding enzyme family. {ECO:0000305}. |
| Subcellular Location | Endoplasmic reticulum {ECO:0000269|PubMed:20237894}. |
| Tissue Specificity | Epidermal-specific expression along the entire stem. In cauline leaves, was expressed over the entire leaf surface, most strongly in trichomes and guard cells, but not in mesophyll cells. In flowers, the expression was detected in the stigma and filaments of the stamens, and in the carpel was expressed specifically in ovaries. In roots, was expressed in primary and lateral roots, but not in the root tips. {ECO:0000269|PubMed:19392700, ECO:0000269|PubMed:20237894}. |
Identical and Related Proteins
Unique RefSeq proteins for LMP010561 (as displayed in Record Overview)
| Protein GI | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| 79324939 | RefSeq | NP_001031554 | 660 | long chain acyl-CoA synthetase 1 |
Identical Sequences to LMP010561 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:79324939 | EMBL | CBF64940.1 | 660 | unnamed protein product [Arabidopsis thaliana] |
| GI:79324939 | EMBL | CBU89811.1 | 660 | unnamed protein product [Arabidopsis thaliana] |
| GI:79324939 | GenBank | ADT60772.1 | 660 | Sequence 2434 from patent US 7847156 |
| GI:79324939 | GenBank | AEC10818.1 | 660 | long chain acyl-CoA synthetase 1 [Arabidopsis thaliana] |
| GI:79324939 | GenBank | AEC10819.1 | 660 | long chain acyl-CoA synthetase 1 [Arabidopsis thaliana] |
| GI:79324939 | GenBank | AGX56840.1 | 660 | Sequence 13999 from patent US 8541208 |
Related Sequences to LMP010561 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:79324939 | GenBank | EFH56548.1 | 660 | long-chain-fatty-acid--CoA ligase family protein [Arabidopsis lyrata subsp. lyrata] |
| GI:79324939 | GenBank | EOA26693.1 | 676 | hypothetical protein CARUB_v10022777mg, partial [Capsella rubella] |
| GI:79324939 | RefSeq | XP_002880289.1 | 660 | long-chain-fatty-acid--CoA ligase family protein [Arabidopsis lyrata subsp. lyrata] |
| GI:79324939 | RefSeq | XP_010507916.1 | 660 | PREDICTED: long chain acyl-CoA synthetase 1-like [Camelina sativa] |
| GI:79324939 | RefSeq | XP_010518417.1 | 660 | PREDICTED: long chain acyl-CoA synthetase 1 isoform X1 [Camelina sativa] |
| GI:79324939 | RefSeq | XP_010518418.1 | 660 | PREDICTED: long chain acyl-CoA synthetase 1 isoform X1 [Camelina sativa] |