Gene/Proteome Database (LMPD)

LMPD ID
LMP010562
Gene ID
841367
Species
Arabidopsis thaliana (Arabidopsis)
Gene Name
long-chain acyl-CoA synthetase 2
Gene Symbol
LACS2
Synonyms
F13F21.14; F13F21_14; LACS2; LATERAL ROOT DEVELOPMENT 2; long-chain acyl-CoA synthetase 2; LRD2
Alternate Names
long-chain acyl-CoA synthetase 2
Chromosome
1
EC Number
6.2.1.3
Summary
Encodes a long chain acyl-CoA synthetase that catalyzes the synthesis of omega-hydroxy fatty acyl-CoA intermediates in the pathway to cutin synthesis. Required for repression of lateral root formation.
Orthologs
View orthologs and multiple alignments for LACS2

Proteins

long-chain acyl-CoA synthetase 2
Refseq ID NP_175368
Protein GI 22330132
UniProt ID Q9XIA9
mRNA ID NM_103833
Length 665
RefSeq Status REVIEWED
MSLAADNVLLVEEGRPATAEHPSAGPVYRCKYAKDGLLDLPTDIDSPWQFFSEAVKKYPNEQMLGQRVTTDSKVGPYTWITYKEAHDAAIRIGSAIRSRGVDPGHCCGIYGANCPEWIIAMEACMSQGITYVPLYDSLGVNAVEFIINHAEVSLVFVQEKTVSSILSCQKGCSSNLKTIVSFGEVSSTQKEEAKNQCVSLFSWNEFSLMGNLDEANLPRKRKTDICTIMYTSGTTGEPKGVILNNAAISVQVLSIDKMLEVTDRSCDTSDVFFSYLPLAHCYDQVMEIYFLSRGSSVGYWRGDIRYLMDDVQALKPTVFCGVPRVYDKLYAGIMQKISASGLIRKKLFDFAYNYKLGNMRKGFSQEEASPRLDRLMFDKIKEALGGRAHMLLSGAAPLPRHVEEFLRIIPASNLSQGYGLTESCGGSFTTLAGVFSMVGTVGVPMPTVEARLVSVPEMGYDAFSADVPRGEICLRGNSMFSGYHKRQDLTDQVLIDGWFHTGDIGEWQEDGSMKIIDRKKNIFKLSQGEYVAVENLENTYSRCPLIAQIWVYGNSFESFLVGVVVPDRKAIEDWAKLNYQSPNDFESLCQNLKAQKYFLDELNSTAKQYQLKGFEMLKAIHLEPNPFDIERDLITPTFKLKRPQLLQHYKGIVDQLYSEAKRSMA

Gene Information

Entrez Gene ID
841367
Gene Name
long-chain acyl-CoA synthetase 2
Gene Symbol
LACS2
Species
Arabidopsis thaliana

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005783 IDA:UniProtKB C endoplasmic reticulum
GO:0009506 IDA:TAIR C plasmodesma
GO:0005524 IEA:UniProtKB-KW F ATP binding
GO:0004467 IDA:UniProtKB F long-chain fatty acid-CoA ligase activity
GO:0031957 IDA:TAIR F very long-chain fatty acid-CoA ligase activity
GO:0010143 IMP:TAIR P cutin biosynthetic process
GO:0050832 IMP:TAIR P defense response to fungus
GO:0006631 TAS:UniProtKB P fatty acid metabolic process
GO:0010311 IMP:TAIR P lateral root formation
GO:0010025 IGI:TAIR P wax biosynthetic process

KEGG Pathway Links

KEGG Pathway ID Description
ath00061 Fatty acid biosynthesis
ath00071 Fatty acid degradation
ath04146 Peroxisome
ath_M00086 beta-Oxidation, acyl-CoA synthesis

Domain Information

InterPro Annotations

Accession Description
IPR020845 AMP-binding, conserved site
IPR000873 AMP-dependent synthetase/ligase

UniProt Annotations

Entry Information

Gene Name
long-chain acyl-CoA synthetase 2
Protein Entry
LACS2_ARATH
UniProt ID
Q9XIA9
Species
Arabidopsis

Comments

Comment Type Description
Catalytic Activity ATP + a long-chain fatty acid + CoA = AMP + diphosphate + an acyl-CoA. {ECO:0000269|PubMed:12177484}.
Cofactor Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
Disruption Phenotype Dwarf phenotype with smaller, wrinkled leaves and overall reduced vigor. Higher water loss rate and susceptibility to drought stress. Defective in the cuticular membrane. Strong resistance to virulent Botrytis cinerea and enhanced susceptibility to avirulent Pseudomonas syringae. {ECO:0000269|PubMed:14973169, ECO:0000269|PubMed:17396154, ECO:0000269|PubMed:17434992, ECO:0000269|PubMed:20237894}.
Function Activation of long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. Acts in the cutin pathway. Preferentially uses palmitate, palmitoleate, oleate and linoleate. Required for repression of lateral root formation through its role in cutin biosynthesis and subsequent aerial tissues permeability. {ECO:0000269|PubMed:14973169, ECO:0000269|PubMed:15960613, ECO:0000269|PubMed:17396154, ECO:0000269|PubMed:17434992, ECO:0000269|PubMed:18952782}.
Induction Positively regulated by WIN1. {ECO:0000269|PubMed:17449808}.
Pathway Lipid metabolism; fatty acid metabolism.
Similarity Belongs to the ATP-dependent AMP-binding enzyme family. {ECO:0000305}.
Subcellular Location Endoplasmic reticulum {ECO:0000269|PubMed:20237894}.
Tissue Specificity Expressed along the entire length of the stem, but expression was not entirely epidermal specific, with some expression found in internal cell layers as well. Was expressed in leave epidermal cells, flowers (sepals, petals, stamens, filaments and carpel), siliques and developing seeds. In roots, expression was detected in an internal cell layer, probably the endodermal layer. {ECO:0000269|PubMed:14973169, ECO:0000269|PubMed:19392700}.

Identical and Related Proteins

Unique RefSeq proteins for LMP010562 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
22330132 RefSeq NP_175368 665 long-chain acyl-CoA synthetase 2

Identical Sequences to LMP010562 proteins

Reference Database Accession Length Protein Name
GI:22330132 EMBL CBF64941.1 665 unnamed protein product [Arabidopsis thaliana]
GI:22330132 EMBL CBU89812.1 665 unnamed protein product [Arabidopsis thaliana]
GI:22330132 GenBank AAN71969.1 665 putative acyl CoA synthetase [Arabidopsis thaliana]
GI:22330132 GenBank AEE32429.1 665 long-chain acyl-CoA synthetase 2 [Arabidopsis thaliana]
GI:22330132 GenBank AGX56841.1 665 Sequence 14001 from patent US 8541208
GI:22330132 SwissProt Q9XIA9.1 665 RecName: Full=Long chain acyl-CoA synthetase 2; AltName: Full=Protein Botrytis resistant 1; AltName: Full=Protein LATERAL ROOT DEVELOPMENT 2 [Arabidopsis thaliana]

Related Sequences to LMP010562 proteins

Reference Database Accession Length Protein Name
GI:22330132 GenBank ABJ40758.1 665 Sequence 15 from patent US 7105722
GI:22330132 GenBank EFH67763.1 665 long-chain acyl-CoA synthetase 2 [Arabidopsis lyrata subsp. lyrata]
GI:22330132 RefSeq XP_002891504.1 665 long-chain acyl-CoA synthetase 2 [Arabidopsis lyrata subsp. lyrata]
GI:22330132 RefSeq XP_010501911.1 663 PREDICTED: long chain acyl-CoA synthetase 2-like, partial [Camelina sativa]
GI:22330132 RefSeq XP_010461777.1 665 PREDICTED: long chain acyl-CoA synthetase 2-like [Camelina sativa]
GI:22330132 RefSeq XP_010479377.1 665 PREDICTED: long chain acyl-CoA synthetase 2 [Camelina sativa]