Gene/Proteome Database (LMPD)

LMPD ID
LMP010571
Gene ID
832432
Species
Arabidopsis thaliana (Arabidopsis)
Gene Name
serine palmitoyltransferase subunit LCB2
Gene Symbol
LCB2
Synonyms
long chain base2; MQM1.6; MQM1_6; SERINE PALMITOYLTRANSFERASE
Alternate Names
serine palmitoyltransferase subunit LCB2
Chromosome
5
EC Number
2.3.1.50

Proteins

serine palmitoyltransferase subunit LCB2
Refseq ID NP_001031932
Protein GI 79328575
UniProt ID Q9LSZ9
mRNA ID NM_001036855
Length 489
RefSeq Status REVIEWED
MITIPYLTAVSTYFSYGLLFAFGQLRDFFRRFIDWWFTSNLQGYAPICLGHEDFYIRRLYHRIQDCFERPISSAPDAWFDVVERYSNDNNKTLKRTTKTSRCLNLGSYNYLGFGSFDEYCTPRVIESLKKFSASTCSSRVDAGTTSVHAELEECVTRFVGKPAAVVFGMGYATNSAIIPVLIGKGGLIISDSLNHSSIVNGARGSGATIRVFQHNTPSHLERVLREQIAEGQPRTHRPWKKIIVVVEGIYSMEGEICHLPEVVAICKKYKAYVYLDEAHSIGAIGKTGKGICELLGVDTADVDVMMGTFTKSFGSCGGYIAGSKELIQYLKHQCPAHLYATSIPTPSAQQIISAIKVILGEDGSNRGAQKLARIRENSNFFRAELQKMGFEVLGDNDSPVMPIMLYNPAKIPAFSRECLRQKVAVVVVGFPATPLLLARARICISASHSREDLIRALKVISKVGDLSGIKYFPAEPKKIEQSKNDIKLD
serine palmitoyltransferase subunit LCB2
Refseq ID NP_197756
Protein GI 15237805
UniProt ID Q9LSZ9
mRNA ID NM_122272
Length 489
RefSeq Status REVIEWED
Protein sequence is identical to GI:79328575 (mRNA isoform)

Gene Information

Entrez Gene ID
832432
Gene Name
serine palmitoyltransferase subunit LCB2
Gene Symbol
LCB2
Species
Arabidopsis thaliana

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005783 IDA:TAIR C endoplasmic reticulum
GO:0016021 IEA:UniProtKB-KW C integral component of membrane
GO:0016020 IDA:TAIR C membrane
GO:0005773 IDA:TAIR C vacuole
GO:0030170 IEA:InterPro F pyridoxal phosphate binding
GO:0004758 IDA:TAIR F serine C-palmitoyltransferase activity
GO:0006915 IEA:UniProtKB-KW P apoptotic process
GO:0009640 IMP:TAIR P photomorphogenesis
GO:0009555 IMP:TAIR P pollen development
GO:0043067 IMP:UniProtKB P regulation of programmed cell death
GO:0046512 IDA:TAIR P sphingosine biosynthetic process

KEGG Pathway Links

KEGG Pathway ID Description
ath01100 Metabolic pathways
ath00600 Sphingolipid metabolism

REACTOME Pathway Links

REACTOME Pathway ID Description
6254191 Sphingolipid de novo biosynthesis
6254192 Sphingolipid metabolism

Domain Information

InterPro Annotations

Accession Description
IPR004839 Aminotransferase, class I/classII
IPR001917 Aminotransferase, class-II, pyridoxal-phosphate binding site
IPR015424 Pyridoxal phosphate-dependent transferase
IPR015421 Pyridoxal phosphate-dependent transferase, major region, subdomain 1
IPR015422 Pyridoxal phosphate-dependent transferase, major region, subdomain 2

UniProt Annotations

Entry Information

Gene Name
serine palmitoyltransferase subunit LCB2
Protein Entry
LCB2A_ARATH
UniProt ID
Q9LSZ9
Species
Arabidopsis

Comments

Comment Type Description
Catalytic Activity Palmitoyl-CoA + L-serine = CoA + 3-dehydro-D- sphinganine + CO(2).
Cofactor Name=pyridoxal 5'-phosphate; Xref=ChEBI:CHEBI:597326; Evidence={ECO:0000250};
Developmental Stage Detected at high levels in the petiole, sepals and petals in young flowers, but was not detected in the anthers during the early stages of pollen development. {ECO:0000269|PubMed:18208516}.
Disruption Phenotype No visible phenotype. Lcb2a and lcb2b double mutant is not viable due to pollen lethality. {ECO:0000269|PubMed:18208516}.
Function Serine palmitoyltransferase (SPT). The heterodimer formed with LCB1 constitutes the catalytic core. Involved in the regulation of the programmed cell death (PCD) signaling pathway. Plays an important role during male gametogenesis and embryogenesis. {ECO:0000269|PubMed:11726713, ECO:0000269|PubMed:18208516, ECO:0000269|PubMed:21534970}.
Miscellaneous The lcb2a-1 mutant is incapable of initiating programmed cell death (PCD) after induction by fumonisin B1 (FB1), a specific inhibitor of ceramide synthase.
Pathway Lipid metabolism; sphingolipid metabolism.
Similarity Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. {ECO:0000305}.
Subcellular Location Endoplasmic reticulum membrane {ECO:0000269|PubMed:11726713}; Single-pass membrane protein {ECO:0000269|PubMed:11726713}.
Subunit Heterodimer with LCB1. Component of the serine palmitoyltransferase (SPT) complex, composed of LCB1 and LCB2 (LCB2a or LCB2b). {ECO:0000269|PubMed:17194770, ECO:0000269|PubMed:18208516}.
Tissue Specificity Ubiquitous. Detected in leaves, roots, stems, flowers and at a lower level in mature seeds. {ECO:0000269|PubMed:11171191, ECO:0000269|PubMed:11726713, ECO:0000269|PubMed:18208516}.

Identical and Related Proteins

Unique RefSeq proteins for LMP010571 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
79328575 RefSeq NP_001031932 489 serine palmitoyltransferase subunit LCB2

Identical Sequences to LMP010571 proteins

Reference Database Accession Length Protein Name
GI:79328575 GenBank AAK92764.1 489 putative serine palmitoyltransferase [Arabidopsis thaliana]
GI:79328575 GenBank AAM91761.1 489 putative serine palmitoyltransferase [Arabidopsis thaliana]
GI:79328575 GenBank ACW88239.1 489 Sequence 5234 from patent US 7569389
GI:79328575 gnl TAIR 489 serine palmitoyltransferase subunit LCB2 [Arabidopsis thaliana]
GI:79328575 gnl TAIR 489 serine palmitoyltransferase subunit LCB2 [Arabidopsis thaliana]
GI:79328575 SwissProt Q9LSZ9.1 489 RecName: Full=Long chain base biosynthesis protein 2a; Short=AtLCB2a; AltName: Full=Long chain base biosynthesis protein 2; Short=AtLCB2 [Arabidopsis thaliana]

Related Sequences to LMP010571 proteins

Reference Database Accession Length Protein Name
GI:79328575 DBBJ BAB03231.1 489 serine palmitoyltransferase [Arabidopsis thaliana]
GI:79328575 GenBank EFH48325.1 489 LCB2 subunit protein [Arabidopsis lyrata subsp. lyrata]
GI:79328575 GenBank EOA20511.1 489 hypothetical protein CARUB_v10000825mg [Capsella rubella]
GI:79328575 RefSeq XP_002872066.1 489 LCB2 subunit protein [Arabidopsis lyrata subsp. lyrata]
GI:79328575 RefSeq XP_006287613.1 489 hypothetical protein CARUB_v10000825mg [Capsella rubella]
GI:79328575 RefSeq XP_010421176.1 489 PREDICTED: long chain base biosynthesis protein 2a [Camelina sativa]