Gene/Proteome Database (LMPD)

LMPD ID
LMP010572
Gene ID
824039
Species
Arabidopsis thaliana (Arabidopsis)
Gene Name
serine palmitoyltransferase 1
Gene Symbol
SPT1
Synonyms
ATSPT1; serine palmitoyltransferase 1; SERINE PALMITOYLTRANSFERASE 1; SPT1
Alternate Names
serine palmitoyltransferase 1
Chromosome
3
EC Number
2.3.1.50
Summary
Encodes one of the two LCB2 subunits (LCB2a and LCB2b) of serine palmitoyltransferase, an enzyme involved in sphingolipid biosynthesis. LCB2a and LCB2b are functional redundant. Double mutants are gametophytic lethal.
Orthologs
View orthologs and multiple alignments for SPT1

Proteins

serine palmitoyltransferase 1
Refseq ID NP_190447
Protein GI 15229023
UniProt ID Q9M304
mRNA ID NM_114737
Length 489
RefSeq Status REVIEWED
MITIPYLTAVSTYFSYGLLFAFGQLRDYSRLIFDWWRTNNLQGYAPICLAHEDFYIRRLYHRIQDCFGRPISSAPDAWIDVVERVSDDNNKTLKRTTKTSRCLNLGSYNYLGFGSFDEYCTPRVIESLKKFSASTCSSRVDAGTTSVHAELEDCVAKYVGQPAAVIFGMGYATNSAIIPVLIGKGGLIISDSLNHTSIVNGARGSGATIRVFQHNTPGHLEKVLKEQIAEGQPRTHRPWKKIIVVVEGIYSMEGEICHLPEIVSICKKYKAYVYLDEAHSIGAIGKTGRGVCELLGVDTSDVDIMMGTFTKSFGSCGGYIAGSKDLIQYLKHQCPAHLYATSISTPSATQIISAIKVILGEDGSNRGAQKLARIRENSNFFRAELQKMGFEVLGDNDSPVMPIMLYNPAKIPAFSRECLRENLAVVVVGFPATPLLLARARICISASHSREDLIKALQVISKAGDLTGIKYFPAAPKKQEVEKNGIKLD

Gene Information

Entrez Gene ID
824039
Gene Name
serine palmitoyltransferase 1
Gene Symbol
SPT1
Species
Arabidopsis thaliana

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005783 IEA:UniProtKB-KW C endoplasmic reticulum
GO:0016021 IEA:UniProtKB-KW C integral component of membrane
GO:0030170 IEA:InterPro F pyridoxal phosphate binding
GO:0004758 IGI:TAIR F serine C-palmitoyltransferase activity
GO:0009640 IMP:TAIR P photomorphogenesis
GO:0009555 IMP:TAIR P pollen development
GO:0030148 IMP:TAIR P sphingolipid biosynthetic process

KEGG Pathway Links

KEGG Pathway ID Description
ath01100 Metabolic pathways
ath00600 Sphingolipid metabolism

REACTOME Pathway Links

REACTOME Pathway ID Description
6254191 Sphingolipid de novo biosynthesis
6254192 Sphingolipid metabolism

Domain Information

InterPro Annotations

Accession Description
IPR004839 Aminotransferase, class I/classII
IPR001917 Aminotransferase, class-II, pyridoxal-phosphate binding site
IPR015424 Pyridoxal phosphate-dependent transferase
IPR015421 Pyridoxal phosphate-dependent transferase, major region, subdomain 1
IPR015422 Pyridoxal phosphate-dependent transferase, major region, subdomain 2

UniProt Annotations

Entry Information

Gene Name
serine palmitoyltransferase 1
Protein Entry
LCB2B_ARATH
UniProt ID
Q9M304
Species
Arabidopsis

Comments

Comment Type Description
Catalytic Activity Palmitoyl-CoA + L-serine = CoA + 3-dehydro-D- sphinganine + CO(2).
Cofactor Name=pyridoxal 5'-phosphate; Xref=ChEBI:CHEBI:597326; Evidence={ECO:0000250};
Developmental Stage In young flower buds was initially restricted to developing pollen spores within the stamen and is not detected in the petals, glumes or petiole until the flowers are mature. {ECO:0000269|PubMed:18208516}.
Disruption Phenotype No visible phenotype. Lcb2a and lcb2b double mutant is not viable due to pollen lethality. {ECO:0000269|PubMed:18208516}.
Function Serine palmitoyltransferase (SPT). The heterodimer formed with LCB1 constitutes the catalytic core. Plays an important role during male gametogenesis and embryogenesis. {ECO:0000269|PubMed:18208516}.
Pathway Lipid metabolism; sphingolipid metabolism.
Similarity Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. {ECO:0000305}.
Subcellular Location Endoplasmic reticulum membrane {ECO:0000250}; Single-pass membrane protein {ECO:0000250}.
Subunit Heterodimer with LCB1. Component of the serine palmitoyltransferase (SPT) complex, composed of LCB1 and LCB2 (LCB2a or LCB2b). {ECO:0000269|PubMed:18208516}.
Tissue Specificity Ubiquitous with the highest expression in flowers. {ECO:0000269|PubMed:18208516}.

Identical and Related Proteins

Unique RefSeq proteins for LMP010572 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
15229023 RefSeq NP_190447 489 serine palmitoyltransferase 1

Identical Sequences to LMP010572 proteins

Reference Database Accession Length Protein Name
GI:15229023 GenBank AAM47981.1 489 serine palmitoyltransferase-like protein [Arabidopsis thaliana]
GI:15229023 GenBank AAP31959.1 489 At3g48780 [Arabidopsis thaliana]
GI:15229023 GenBank ACW88201.1 489 Sequence 5182 from patent US 7569389
GI:15229023 GenBank ACW88537.1 489 Sequence 5637 from patent US 7569389
GI:15229023 GenBank AEE78455.1 489 serine palmitoyltransferase 1 [Arabidopsis thaliana]
GI:15229023 SwissProt Q9M304.1 489 RecName: Full=Long chain base biosynthesis protein 2b; Short=AtLCB2b; AltName: Full=Serine palmitoyltransferase 1; Short=AtSPT1 [Arabidopsis thaliana]

Related Sequences to LMP010572 proteins

Reference Database Accession Length Protein Name
GI:15229023 GenBank EFH52184.1 489 hypothetical protein ARALYDRAFT_485232 [Arabidopsis lyrata subsp. lyrata]
GI:15229023 GenBank EOA23885.1 489 hypothetical protein CARUB_v10017101mg [Capsella rubella]
GI:15229023 RefSeq XP_002875925.1 489 hypothetical protein ARALYDRAFT_485232 [Arabidopsis lyrata subsp. lyrata]
GI:15229023 RefSeq XP_006290987.1 489 hypothetical protein CARUB_v10017101mg [Capsella rubella]
GI:15229023 RefSeq XP_010515202.1 489 PREDICTED: long chain base biosynthesis protein 2b [Camelina sativa]
GI:15229023 RefSeq XP_010426376.1 489 PREDICTED: long chain base biosynthesis protein 2b [Camelina sativa]