Gene/Proteome Database (LMPD)

LMPD ID
LMP010596
Gene ID
820862
Species
Arabidopsis thaliana (Arabidopsis)
Gene Name
malonate--CoA ligase
Gene Symbol
AT3G16170
Alternate Names
malonate--CoA ligase
Chromosome
3
EC Number
6.2.1.n3

Proteins

malonate--CoA ligase
Refseq ID NP_566537
Protein GI 18401035
UniProt ID Q8H151
mRNA ID NM_112487
Length 544
RefSeq Status REVIEWED
MEVFKAAFSEASNSCDRIAIKADGKSYSYGQLTSSALRISKLFLKDDTTNGGQETKKYEGFGSLKGARIGIVAKPSAEFVAGVLGTWFSGGVAVPLALSYPEAELLHVMNDSDISLLLSTEDHSETMKTIAAKSGARFHLIPPVVNSTSETVACNQFQDDSFEAEGKFLDDPALIVYTSGTTGKPKGVVHTHNSINSQVRMLTEAWEYTSADHFLHCLPLHHVHGLFNALFAPLYARSLVEFLPKFSVSGIWRRWRESYPVNDEKTNDSITVFTGVPTMYTRLIQGYEAMDKEMQDSSAFAARKLRLMMSGSSALPRPVMHQWESITGHRLLERYGMTEFVMAMSNPLRGARNAGTVGKPLPGVEAKIKEDENDANGVGEICVKSPSLFKEYWNLPEVTKESFTEDGYFKTGDAGRVDEDGYYVILGRNSADIMKVGGYKLSALEIESTLLEHPTVAECCVLGLTDNDYGEAVTAIIIAESAAKKRREDESKPVITLEELCGWAKDKLAPYKLPTRLLIWESLPRNAMGKVNKKELKKSLENQE

Gene Information

Entrez Gene ID
820862
Gene Name
malonate--CoA ligase
Gene Symbol
AT3G16170
Species
Arabidopsis thaliana

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005829 IDA:TAIR C cytosol
GO:0016020 IDA:TAIR C membrane
GO:0005634 IDA:TAIR C nucleus
GO:0090409 IDA:TAIR F malonyl-CoA synthetase activity
GO:0006631 IEA:UniProtKB-KW P fatty acid metabolic process
GO:0090410 IMP:TAIR P malonate catabolic process

Domain Information

InterPro Annotations

Accession Description
IPR025110 AMP-binding enzyme C-terminal domain
IPR020845 AMP-binding, conserved site
IPR000873 AMP-dependent synthetase/ligase

UniProt Annotations

Entry Information

Gene Name
malonate--CoA ligase
Protein Entry
AAE13_ARATH
UniProt ID
Q8H151
Species
Arabidopsis

Comments

Comment Type Description
Biophysicochemical Properties Kinetic parameters: KM=530 uM for malonate (at pH 7.5) {ECO:0000269|PubMed:21642549}; Vmax=24 umol/min/mg enzyme toward malonate (at pH 7.5) {ECO:0000269|PubMed:21642549}; pH dependence: Optimum pH is 6.5-8.0. {ECO:0000269|PubMed:21642549};
Catalytic Activity ATP + malonate + CoA = AMP + diphosphate + malonyl-CoA. {ECO:0000269|PubMed:21642549}.
Disruption Phenotype Strong defects in growth and development and most of the plants die before flowering. Viable plants have a greatly reduced seed set with a low proportion of viable seed and accumulate malonate and succinate. {ECO:0000269|PubMed:21642549}.
Function Malonate--CoA ligase that catalyzes the formation of malonyl-CoA directly from malonate and CoA. May be required for the detoxification of malonate. {ECO:0000269|PubMed:21642549}.
Induction By abscisic acid (ABA) and osmotic stress. {ECO:0000269|PubMed:21642549}.
Miscellaneous Plants overexpressing AAE13 show increased sensitivity to exogenous malonate.
Sequence Caution Sequence=BAB02683.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
Similarity Belongs to the ATP-dependent AMP-binding enzyme family. {ECO:0000305}.
Subcellular Location Cytoplasm {ECO:0000269|PubMed:21642549}. Nucleus {ECO:0000269|PubMed:21642549}.
Tissue Specificity Expressed in flowers. {ECO:0000269|PubMed:12805634, ECO:0000269|PubMed:21642549}.

Identical and Related Proteins

Unique RefSeq proteins for LMP010596 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
18401035 RefSeq NP_566537 544 malonate--CoA ligase

Identical Sequences to LMP010596 proteins

Reference Database Accession Length Protein Name
GI:18401035 GenBank AAN31910.1 544 putative long-chain acyl-CoA synthetase [Arabidopsis thaliana]
GI:18401035 GenBank AAP03025.1 544 acyl-activating enzyme 13 [Arabidopsis thaliana]
GI:18401035 GenBank AEE75779.1 544 malonate--CoA ligase [Arabidopsis thaliana]
GI:18401035 SwissProt Q8H151.1 544 RecName: Full=Malonate--CoA ligase; AltName: Full=Acyl-activating enzyme 13; AltName: Full=Malonyl-CoA synthetase [Arabidopsis thaliana]

Related Sequences to LMP010596 proteins

Reference Database Accession Length Protein Name
GI:18401035 DBBJ BAB02683.1 608 long-chain-fatty-acid-CoA ligase-like protein [Arabidopsis thaliana]
GI:18401035 GenBank AAM61199.1 544 putative long-chain acyl-CoA synthetase [Arabidopsis thaliana]
GI:18401035 GenBank EFH59242.1 608 hypothetical protein ARALYDRAFT_318392 [Arabidopsis lyrata subsp. lyrata]
GI:18401035 GenBank EOA30153.1 606 hypothetical protein CARUB_v10013261mg [Capsella rubella]
GI:18401035 RefSeq XP_002882983.1 608 hypothetical protein ARALYDRAFT_318392 [Arabidopsis lyrata subsp. lyrata]
GI:18401035 RefSeq XP_006297255.1 606 hypothetical protein CARUB_v10013261mg [Capsella rubella]