Gene/Proteome Database (LMPD)

LMPD ID
LMP010620
Gene ID
828374
Species
Arabidopsis thaliana (Arabidopsis)
Gene Name
sterol C4-methyl oxidase 1-2
Gene Symbol
SMO1-2
Synonyms
ATSMO1; ATSMO1-2; SMO1; SMO1-2; STEROL 4-ALPHA-METHYL-OXIDASE 1; STEROL C4-METHYL OXIDASE 1; sterol C4-methyl oxidase 1-2
Alternate Names
sterol C4-methyl oxidase 1-2
Chromosome
4
EC Number
1.14.13.72
Summary
Encodes a member of the SMO1 family of sterol 4alpha-methyl oxidases. More specifically functions as a 4,4-dimethyl-9beta,19-cyclopropylsterol-4alpha- methyl oxidase.
Orthologs
View orthologs and multiple alignments for SMO1-2

Proteins

sterol C4-methyl oxidase 1-2
Refseq ID NP_567670
Protein GI 18416004
UniProt ID Q1EC69
mRNA ID NM_118404
Length 299
RefSeq Status REVIEWED
MIPYATIEEASIALSRNLTWLETLWFDYSATKSDYYLYCHNILFLFLIFSLVPLPLVFIESSQSTSDLFNRYKIQPKVKNSFSSMFKCYKDVMKMFILVVGPLQLVSYPSIQMIEIRSGLPLPSCMEIVAQLVVYFLVEDYTNYWVHRFFHCKWGYEKFHHIHHEYTAPIGYAAPYAHWAEVLLLGIPTFLGPAIAPGHMITFWLWIALRQIEAIETHSGYDFPWSLTKYIPFYGGAEYHDYHHYVGGQSQSNFASVFTYCDYIYGTDKGYRFQKKLLQQMKEKSKKSNKLVNGGEKFD

Gene Information

Entrez Gene ID
828374
Gene Name
sterol C4-methyl oxidase 1-2
Gene Symbol
SMO1-2
Species
Arabidopsis thaliana

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005783 IEA:UniProtKB-KW C endoplasmic reticulum
GO:0016021 IEA:UniProtKB-KW C integral component of membrane
GO:0000254 IMP:TAIR F C-4 methylsterol oxidase activity
GO:0005506 IEA:InterPro F iron ion binding
GO:0006633 IEA:InterPro P fatty acid biosynthetic process
GO:0016126 IMP:TAIR P sterol biosynthetic process

KEGG Pathway Links

KEGG Pathway ID Description
ath01100 Metabolic pathways
ath00100 Steroid biosynthesis

REACTOME Pathway Links

REACTOME Pathway ID Description
6254042 Bile acid and bile salt metabolism
6254041 Synthesis of bile acids and bile salts

Domain Information

InterPro Annotations

Accession Description
IPR006694 Fatty_acid_hydroxylase

UniProt Annotations

Entry Information

Gene Name
sterol C4-methyl oxidase 1-2
Protein Entry
SMO12_ARATH
UniProt ID
Q1EC69
Species
Arabidopsis

Comments

Comment Type Description
Catalytic Activity 3-beta-hydroxy-4-beta-methyl-5-alpha-cholest- 7-ene-4-alpha-carbaldehyde + NAD(P)H + O(2) = 3-beta-hydroxy-4- beta-methyl-5-alpha-cholest-7-ene-4-alpha-carboxylate + NAD(P)(+) + H(2)O.
Catalytic Activity 4,4-dimethyl-5-alpha-cholest-7-en-3-beta-ol + NAD(P)H + O(2) = 4-beta-hydroxymethyl-4-alpha-methyl-5-alpha- cholest-7-en-3-beta-ol + NAD(P)(+) + H(2)O.
Catalytic Activity 4-beta-hydroxymethyl-4-alpha-methyl-5-alpha- cholest-7-en-3-beta-ol + NAD(P)H + O(2) = 3-beta-hydroxy-4-beta- methyl-5-alpha-cholest-7-ene-4-alpha-carbaldehyde + NAD(P)(+) + 2 H(2)O.
Cofactor Name=Fe cation; Xref=ChEBI:CHEBI:24875; Evidence={ECO:0000250};
Domain The histidine box domains may contain the active site and/or be involved in metal ion binding.
Function Non-heme iron oxygenase involved in sterols biosynthesis. 4,4-dimethyl-9-beta,19-cyclopropylsterols such as 24-methylenecycloartanol are the preferred substratess.
Miscellaneous Requires a membrane-bound cytochrome b5 as an obligatory electron carrier from NAD(P)H to SMO. {ECO:0000250}.
Similarity Belongs to the sterol desaturase family. {ECO:0000305}.
Subcellular Location Endoplasmic reticulum membrane {ECO:0000250}; Multi-pass membrane protein {ECO:0000250}.

Identical and Related Proteins

Unique RefSeq proteins for LMP010620 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
18416004 RefSeq NP_567670 299 sterol C4-methyl oxidase 1-2

Identical Sequences to LMP010620 proteins

Reference Database Accession Length Protein Name
GI:18416004 EMBL CAR81289.1 299 unnamed protein product [Arabidopsis thaliana]
GI:18416004 EMBL CBX84767.1 299 unnamed protein product [Arabidopsis thaliana]
GI:18416004 GenBank ACX17354.1 299 Sequence 44802 from patent US 7569389
GI:18416004 GenBank AEE84653.1 299 sterol C4-methyl oxidase 1-2 [Arabidopsis thaliana]
GI:18416004 GenBank AGD16826.1 299 Sequence 14282 from patent US 8343764
GI:18416004 SwissProt Q1EC69.1 299 RecName: Full=Methylsterol monooxygenase 1-2; AltName: Full=Sterol 4-alpha-methyl-oxidase 1-2; Short=AtSMO1-2 [Arabidopsis thaliana]

Related Sequences to LMP010620 proteins

Reference Database Accession Length Protein Name
GI:18416004 EMBL CAR81340.1 299 unnamed protein product [Arabidopsis thaliana]
GI:18416004 EMBL CBX84816.1 299 unnamed protein product [Arabidopsis thaliana]
GI:18416004 GenBank AAK61361.1 299 putative sterol 4-alpha-methyl-oxidase [Arabidopsis thaliana]
GI:18416004 GenBank ACX17353.1 338 Sequence 44801 from patent US 7569389
GI:18416004 GenBank ACX21852.1 299 Sequence 50887 from patent US 7569389
GI:18416004 GenBank AGD20793.1 299 Sequence 18249 from patent US 8343764