Gene/Proteome Database (LMPD)

LMPD ID
LMP010622
Gene ID
817488
Species
Arabidopsis thaliana (Arabidopsis)
Gene Name
sterol 4-alpha-methyl-oxidase 2-2
Gene Symbol
SMO2-2
Synonyms
ATSMO2; F16P2.23; F16P2_23; SMO2-2; STEROL 4-ALPHA-METHYL-OXIDASE 2; sterol 4-alpha-methyl-oxidase 2-2
Alternate Names
sterol 4-alpha-methyl-oxidase 2-2
Chromosome
2
EC Number
1.14.13.72
Summary
Encodes a sterol 4-alpha-methyl-oxidase, specifically a 4-alpha-methyl-delta-7-sterol-4alpha-methyl-oxidase.
Orthologs
View orthologs and multiple alignments for SMO2-2

Proteins

sterol 4-alpha-methyl-oxidase 2-2
Refseq ID NP_850133
Protein GI 30684225
UniProt ID Q9ZW22
mRNA ID NM_179802
Length 260
RefSeq Status REVIEWED
MDSLVESGWKYLVTHFSDFQLACIGSFILHESVFFLSGLPYIFLERTGFLSNYKIQTKSNTPEAQGKCIARLLLYHCCVNLPLMMASYPVFRFMGMESSFPLPSWKVVSAQILFYFIIEDFVFYWGHRILHTKWLYKNVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHLITLWLWMMLRVIETVEAHCGYHFPWSPSNFLPLYGGADFHDYHHRLLYTKSGNYSSTFVYMDWIFGTDKGYRKLKALKET
sterol 4-alpha-methyl-oxidase 2-2
Refseq ID NP_565681
Protein GI 18402028
UniProt ID Q9ZW22
mRNA ID NM_128493
Length 272
RefSeq Status REVIEWED
MDSLVESGWKYLVTHFSDFQLACIGSFILHESVFFLSGLPYIFLERTGFLSNYKIQTKSNTPEAQGKCIARLLLYHCCVNLPLMMASYPVFRFMGMESSFPLPSWKVVSAQILFYFIIEDFVFYWGHRILHTKWLYKNVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHLITLWLWMMLRVIETVEAHCGYHFPWSPSNFLPLYGGSLILMWESFAYSADFHDYHHRLLYTKSGNYSSTFVYMDWIFGTDKGYRKLKALKET
sterol 4-alpha-methyl-oxidase 2-2
Refseq ID NP_001077975
Protein GI 145329979
UniProt ID Q9ZW22
mRNA ID NM_001084506
Length 219
RefSeq Status REVIEWED
MERYHQVVSVLISPMTKSNTPEAQGKCIARLLLYHCCVNLPLMMASYPVFRFMGMESSFPLPSWKVVSAQILFYFIIEDFVFYWGHRILHTKWLYKNVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHLITLWLWMMLRVIETVEAHCGYHFPWSPSNFLPLYGGADFHDYHHRLLYTKSGNYSSTFVYMDWIFGTDKGYRKLKALKET
sterol 4-alpha-methyl-oxidase 2-2
Refseq ID NP_001077974
Protein GI 145329977
UniProt ID Q9ZW22
mRNA ID NM_001084505
Length 177
RefSeq Status REVIEWED
MMASYPVFRFMGMESSFPLPSWKVVSAQILFYFIIEDFVFYWGHRILHTKWLYKNVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHLITLWLWMMLRVIETVEAHCGYHFPWSPSNFLPLYGGADFHDYHHRLLYTKSGNYSSTFVYMDWIFGTDKGYRKLKALKET
sterol 4-alpha-methyl-oxidase 2-2
Refseq ID NP_973559
Protein GI 42570971
UniProt ID Q9ZW22
mRNA ID NM_201830
Length 253
RefSeq Status REVIEWED
MMQYLVTHFSDFQLACIGSFILHESVFFLSGLPYIFLERTGFLSNYKIQTKSNTPEAQGKCIARLLLYHCCVNLPLMMASYPVFRFMGMESSFPLPSWKVVSAQILFYFIIEDFVFYWGHRILHTKWLYKNVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHLITLWLWMMLRVIETVEAHCGYHFPWSPSNFLPLYGGADFHDYHHRLLYTKSGNYSSTFVYMDWIFGTDKGYRKLKALKET

Gene Information

Entrez Gene ID
817488
Gene Name
sterol 4-alpha-methyl-oxidase 2-2
Gene Symbol
SMO2-2
Species
Arabidopsis thaliana

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005783 IEA:UniProtKB-KW C endoplasmic reticulum
GO:0016021 IEA:UniProtKB-KW C integral component of membrane
GO:0016020 TAS:TAIR C membrane
GO:0000254 IGI:TAIR F C-4 methylsterol oxidase activity
GO:0005506 IEA:InterPro F iron ion binding
GO:0006633 IEA:InterPro P fatty acid biosynthetic process
GO:0016126 IGI:TAIR P sterol biosynthetic process

KEGG Pathway Links

KEGG Pathway ID Description
ath01100 Metabolic pathways
ath00100 Steroid biosynthesis

REACTOME Pathway Links

REACTOME Pathway ID Description
6254036 Cholesterol biosynthesis
6254041 Synthesis of bile acids and bile salts

Domain Information

InterPro Annotations

Accession Description
IPR006694 Fatty_acid_hydroxylase

UniProt Annotations

Entry Information

Gene Name
sterol 4-alpha-methyl-oxidase 2-2
Protein Entry
SMO21_ARATH
UniProt ID
Q9ZW22
Species
Arabidopsis

Comments

Comment Type Description
Alternative Products Event=Alternative splicing; Named isoforms=5; Name=1; IsoId=Q9ZW22-1; Sequence=Displayed; Name=2; IsoId=Q9ZW22-2; Sequence=VSP_041864; Note=No experimental confirmation available.; Name=3; IsoId=Q9ZW22-3; Sequence=VSP_041862, VSP_041864; Note=No experimental confirmation available.; Name=4; IsoId=Q9ZW22-4; Sequence=VSP_041860, VSP_041864; Note=Derived from EST data. No experimental confirmation available.; Name=5; IsoId=Q9ZW22-5; Sequence=VSP_041861, VSP_041863, VSP_041864; Note=Derived from EST data.;
Catalytic Activity 3-beta-hydroxy-4-beta-methyl-5-alpha-cholest- 7-ene-4-alpha-carbaldehyde + NAD(P)H + O(2) = 3-beta-hydroxy-4- beta-methyl-5-alpha-cholest-7-ene-4-alpha-carboxylate + NAD(P)(+) + H(2)O.
Catalytic Activity 4,4-dimethyl-5-alpha-cholest-7-en-3-beta-ol + NAD(P)H + O(2) = 4-beta-hydroxymethyl-4-alpha-methyl-5-alpha- cholest-7-en-3-beta-ol + NAD(P)(+) + H(2)O.
Catalytic Activity 4-beta-hydroxymethyl-4-alpha-methyl-5-alpha- cholest-7-en-3-beta-ol + NAD(P)H + O(2) = 3-beta-hydroxy-4-beta- methyl-5-alpha-cholest-7-ene-4-alpha-carbaldehyde + NAD(P)(+) + 2 H(2)O.
Cofactor Name=Fe cation; Xref=ChEBI:CHEBI:24875; Evidence={ECO:0000250};
Domain The histidine box domains may contain the active site and/or be involved in metal ion binding.
Function Non-heme iron oxygenase involved in sterols biosynthesis. 24-ethylidenelophenol and 24-ethyllophenol are the preferred substrates. {ECO:0000269|PubMed:11707264}.
Miscellaneous Requires a membrane-bound cytochrome b5 as an obligatory electron carrier from NAD(P)H to SMO.
Similarity Belongs to the sterol desaturase family. {ECO:0000305}.
Subcellular Location Endoplasmic reticulum membrane {ECO:0000250}; Multi-pass membrane protein {ECO:0000250}.

Identical and Related Proteins

Unique RefSeq proteins for LMP010622 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
18402028 RefSeq NP_565681 272 sterol 4-alpha-methyl-oxidase 2-2
30684225 RefSeq NP_850133 260 sterol 4-alpha-methyl-oxidase 2-2
42570971 RefSeq NP_973559 253 sterol 4-alpha-methyl-oxidase 2-2
145329979 RefSeq NP_001077975 219 sterol 4-alpha-methyl-oxidase 2-2
145329977 RefSeq NP_001077974 177 sterol 4-alpha-methyl-oxidase 2-2

Identical Sequences to LMP010622 proteins

Reference Database Accession Length Protein Name
GI:30684225 EMBL CAR81291.1 260 unnamed protein product [Arabidopsis thaliana]
GI:42570971 EMBL CAR81294.1 253 unnamed protein product [Arabidopsis thaliana]
GI:18402028 EMBL CBX84765.1 272 unnamed protein product [Arabidopsis thaliana]
GI:30684225 EMBL CBX84769.1 260 unnamed protein product [Arabidopsis thaliana]
GI:42570971 EMBL CBX84772.1 253 unnamed protein product [Arabidopsis thaliana]
GI:30684225 GenBank AAO22608.1 260 putative C-4 sterol methyl oxidase [Arabidopsis thaliana]
GI:30684225 GenBank AAO50658.1 260 putative C-4 sterol methyl oxidase [Arabidopsis thaliana]
GI:42570971 GenBank ACX16751.1 253 Sequence 43987 from patent US 7569389
GI:145329977 GenBank ACX16753.1 177 Sequence 43989 from patent US 7569389
GI:30684225 GenBank ACX18476.1 260 Sequence 46322 from patent US 7569389
GI:145329977 GenBank ACX18477.1 177 Sequence 46323 from patent US 7569389
GI:18402028 GenBank AEC08245.1 272 sterol 4-alpha-methyl-oxidase 2-2 [Arabidopsis thaliana]
GI:42570971 GenBank AEC08246.1 253 sterol 4-alpha-methyl-oxidase 2-2 [Arabidopsis thaliana]
GI:30684225 GenBank AEC08247.1 260 sterol 4-alpha-methyl-oxidase 2-2 [Arabidopsis thaliana]
GI:145329977 GenBank AEC08248.1 177 sterol 4-alpha-methyl-oxidase 2-2 [Arabidopsis thaliana]
GI:145329979 GenBank AEC08249.1 219 sterol 4-alpha-methyl-oxidase 2-2 [Arabidopsis thaliana]
GI:18402028 GenBank AEL85224.1 272 Sequence 902 from patent US 7989676
GI:18402028 GenBank AFX28348.1 272 Sequence 9408 from patent US 8299318
GI:18402028 GenBank AFX49608.1 272 Sequence 51441 from patent US 8299318
GI:18402028 GenBank AGF14085.1 272 Sequence 13504 from patent US 8362325

Related Sequences to LMP010622 proteins

Reference Database Accession Length Protein Name
GI:18402028 EMBL CAR81291.1 260 unnamed protein product [Arabidopsis thaliana]
GI:145329977 EMBL CAR81294.1 253 unnamed protein product [Arabidopsis thaliana]
GI:145329979 EMBL CAR81294.1 253 unnamed protein product [Arabidopsis thaliana]
GI:18402028 EMBL CBX84769.1 260 unnamed protein product [Arabidopsis thaliana]
GI:42570971 EMBL CBX84769.1 260 unnamed protein product [Arabidopsis thaliana]
GI:145329979 EMBL CBX84772.1 253 unnamed protein product [Arabidopsis thaliana]
GI:145329977 EMBL CBX84772.1 253 unnamed protein product [Arabidopsis thaliana]
GI:18402028 GenBank AAL32302.1 260 sterol 4-alpha-methyl-oxidase [Arabidopsis thaliana]
GI:42570971 GenBank AAL32302.1 260 sterol 4-alpha-methyl-oxidase [Arabidopsis thaliana]
GI:30684225 GenBank AAM64359.1 272 putative C-4 sterol methyl oxidase [Arabidopsis thaliana]
GI:145329979 GenBank AAO50658.1 260 putative C-4 sterol methyl oxidase [Arabidopsis thaliana]
GI:18402028 GenBank AAO50658.1 260 putative C-4 sterol methyl oxidase [Arabidopsis thaliana]
GI:42570971 GenBank AAO50658.1 260 putative C-4 sterol methyl oxidase [Arabidopsis thaliana]
GI:145329977 GenBank AAO50658.1 260 putative C-4 sterol methyl oxidase [Arabidopsis thaliana]
GI:145329977 GenBank ACX16751.1 253 Sequence 43987 from patent US 7569389
GI:145329979 GenBank ACX16751.1 253 Sequence 43987 from patent US 7569389
GI:42570971 GenBank ACX16752.1 252 Sequence 43988 from patent US 7569389
GI:30684225 GenBank ACX18475.1 281 Sequence 46321 from patent US 7569389
GI:30684225 GenBank EFH57299.1 260 hypothetical protein ARALYDRAFT_481856 [Arabidopsis lyrata subsp. lyrata]
GI:145329979 GenBank AEC08246.1 253 sterol 4-alpha-methyl-oxidase 2-2 [Arabidopsis thaliana]
GI:145329977 GenBank AEC08246.1 253 sterol 4-alpha-methyl-oxidase 2-2 [Arabidopsis thaliana]
GI:18402028 GenBank AEC08247.1 260 sterol 4-alpha-methyl-oxidase 2-2 [Arabidopsis thaliana]
GI:42570971 GenBank AEC08247.1 260 sterol 4-alpha-methyl-oxidase 2-2 [Arabidopsis thaliana]
GI:30684225 gnl TIGR 272 putative C-4 sterol methyl oxidase [Arabidopsis thaliana]
GI:30684225 RefSeq NP_565681.1 272 sterol 4-alpha-methyl-oxidase 2-2 [Arabidopsis thaliana]
GI:18402028 RefSeq NP_850133.1 260 sterol 4-alpha-methyl-oxidase 2-2 [Arabidopsis thaliana]
GI:42570971 RefSeq NP_850133.1 260 sterol 4-alpha-methyl-oxidase 2-2 [Arabidopsis thaliana]
GI:145329977 RefSeq NP_973559.1 253 sterol 4-alpha-methyl-oxidase 2-2 [Arabidopsis thaliana]
GI:145329979 RefSeq NP_973559.1 253 sterol 4-alpha-methyl-oxidase 2-2 [Arabidopsis thaliana]
GI:30684225 RefSeq XP_002881040.1 260 hypothetical protein ARALYDRAFT_481856 [Arabidopsis lyrata subsp. lyrata]