Gene/Proteome Database (LMPD)
LMPD ID
LMP010684
Gene ID
Species
Arabidopsis thaliana (Arabidopsis)
Gene Name
nuclear transcription factor Y subunit B-9
Gene Symbol
Synonyms
EMB 212; EMB212; EMBRYO DEFECTIVE 212; LEAFY COTYLEDON 1; LEC1; NF-YB9; NUCLEAR FACTOR Y; SUBUNIT B9; T26F17.20; T26F17_20
Alternate Names
nuclear transcription factor Y subunit B-9
Chromosome
1
Summary
Transcriptional activator of genes required for both embryo maturation and cellular differentiation. Sequence is similar to HAP3 subunit of the CCAAT-box binding factor. HAP3 subunit is divided into three domains: an amino-terminal A domain, a central B domain, and a carboxyl-terminal C domain. LEC1 shared high similarity with other HAP3 homologs only in central, B domain. LEC1 is required for the specification of cotyledon identity and the completion of embryo maturation. It was sufficient to induce embryogenic programs in vegetative cells, suggesting that LEC1 is a major embryonic regulator that mediates the switch between embryo and vegetative development. Mutants are desiccation intolerant, have trichomes on cotyledons and exhibit precocious meristem activation. Levels of the ABI3 and FUS3 transcripts were significantly reduced in developing siliques of the lec1-1 mutants, indicating that LEC1 down-regulates FUS3 and ABI3.When LEC1 is overexpressed from an inducible promoter, the expression of numerous genes involved in fatty acid biosynthesis is increased suggesting a role in positive regulation of FA biosynthesis.
Orthologs
Proteins
| nuclear transcription factor Y subunit B-9 | |
|---|---|
| Refseq ID | NP_173616 |
| Protein GI | 42562232 |
| UniProt ID | Q9SFD8 |
| mRNA ID | NM_102046 |
| Length | 238 |
| RefSeq Status | REVIEWED |
| MERGAPFSHYQLPKSISELNLDQHSNNPTPMTSSVVVAGAGDKNNGIVVQQQPPCVAREQDQYMPIANVIRIMRKTLPSHAKISDDAKETIQECVSEYISFVTGEANERCQREQRKTITAEDILWAMSKLGFDNYVDPLTVFINRYREIETDRGSALRGEPPSLRQTYGGNGIGFHGPSHGLPPPGPYGYGMLDQSMVMGGGRYYQNGSSGQDESSVGGGSSSSINGMPAFDHYGQYK | |
Gene Information
Entrez Gene ID
Gene Name
nuclear transcription factor Y subunit B-9
Gene Symbol
Species
Arabidopsis thaliana
Gene Ontology (GO Annotations)
| GO ID | Source | Type | Description |
|---|---|---|---|
| GO:0005634 | IEA:UniProtKB-KW | C | nucleus |
| GO:0043565 | IEA:InterPro | F | sequence-specific DNA binding |
| GO:0003700 | ISS:TAIR | F | sequence-specific DNA binding transcription factor activity |
| GO:0009738 | IMP:UniProtKB | P | abscisic acid-activated signaling pathway |
| GO:0009785 | IMP:UniProtKB | P | blue light signaling pathway |
| GO:0009793 | IMP:TAIR | P | embryo development ending in seed dormancy |
| GO:0045723 | IMP:TAIR | P | positive regulation of fatty acid biosynthetic process |
| GO:0045893 | IDA:TAIR | P | positive regulation of transcription, DNA-templated |
| GO:0010262 | IMP:TAIR | P | somatic embryogenesis |
| GO:0006351 | IEA:UniProtKB-KW | P | transcription, DNA-templated |
REACTOME Pathway Links
Domain Information
UniProt Annotations
Entry Information
Gene Name
nuclear transcription factor Y subunit B-9
Protein Entry
NFYB9_ARATH
UniProt ID
Species
Arabidopsis
Comments
| Comment Type | Description |
|---|---|
| Developmental Stage | Accumulates during seed development in embryo cell types and in endosperm tissue. {ECO:0000269|PubMed:9657152}. |
| Disruption Phenotype | Altered response to blue light (BL) and abscisic acid (ABA). {ECO:0000269|PubMed:17322342}. |
| Function | Component of the NF-Y/HAP transcription factor complex. The NF-Y complex stimulates the transcription of various genes by recognizing and binding to a CCAAT motif in promoters. Acts as a central regulator of the embryogenesis. Required for the speciation of cotyledon identity and the completion of embryo maturation. Controls seed storage protein genes through the regulation of FUS3 and ABI3. Involved in the blue light (BL) and abscisic acid (ABA) signaling pathways. {ECO:0000269|PubMed:12578989, ECO:0000269|PubMed:15695450, ECO:0000269|PubMed:17322342, ECO:0000269|PubMed:9657152}. |
| Sequence Caution | Sequence=AAC39488.1; Type=Erroneous initiation; Note=Translation N-terminally extended.; Evidence={ECO:0000305}; Sequence=AAF16537.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305}; |
| Similarity | Belongs to the NFYB/HAP3 subunit family. {ECO:0000305}. |
| Subcellular Location | Nucleus {ECO:0000305}. |
| Subunit | Heterotrimeric transcription factor composed of three components, NF-YA, NF-YB and NF-YC. NF-YB and NF-YC must interact and dimerize for NF-YA association and DNA binding (By similarity). Interacts with PRN1. {ECO:0000250, ECO:0000269|PubMed:17322342}. |
| Tissue Specificity | Expressed in green siliques. Present in etiolated seedlings. {ECO:0000269|PubMed:11250072, ECO:0000269|PubMed:17322342}. |
Identical and Related Proteins
Unique RefSeq proteins for LMP010684 (as displayed in Record Overview)
| Protein GI | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| 42562232 | RefSeq | NP_173616 | 238 | nuclear transcription factor Y subunit B-9 |
Identical Sequences to LMP010684 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:42562232 | GenBank | AEE30179.1 | 238 | nuclear transcription factor Y subunit B-9 [Arabidopsis thaliana] |
| GI:42562232 | SwissProt | Q9SFD8.2 | 238 | RecName: Full=Nuclear transcription factor Y subunit B-9; Short=AtNF-YB-9; AltName: Full=Protein LEAFY COTYLEDON 1 [Arabidopsis thaliana] |
Related Sequences to LMP010684 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:42562232 | DBBJ | BAH30312.1 | 235 | hypothetical protein, partial [Arabidopsis thaliana] |
| GI:42562232 | GenBank | ABY02690.1 | 208 | Sequence 195 from patent US 7294759 |
| GI:42562232 | GenBank | EFH38915.1 | 236 | hypothetical protein ARALYDRAFT_497363 [Arabidopsis lyrata subsp. lyrata] |
| GI:42562232 | GenBank | EFH69463.1 | 237 | hypothetical protein ARALYDRAFT_472432 [Arabidopsis lyrata subsp. lyrata] |
| GI:42562232 | RefSeq | XP_002862657.1 | 236 | hypothetical protein ARALYDRAFT_497363 [Arabidopsis lyrata subsp. lyrata] |
| GI:42562232 | RefSeq | XP_002893204.1 | 237 | hypothetical protein ARALYDRAFT_472432 [Arabidopsis lyrata subsp. lyrata] |