Gene/Proteome Database (LMPD)
LMPD ID
LMP010758
Gene ID
Species
Arabidopsis thaliana (Arabidopsis)
Gene Name
fatty acid beta-oxidation multifunctional protein MFP2
Gene Symbol
Synonyms
ATMFP2; F3E22.20; MFP2; multifunctional protein 2; MULTIFUNCTIONAL PROTEIN 2
Alternate Names
fatty acid beta-oxidation multifunctional protein MFP2
Chromosome
3
EC Number
4.2.1.17
Summary
Encodes a multifunctional protein. Involved in peroxisomal fatty acid beta oxidation. Loss-of-function mutant lacks hydroxyacyl-CoA dehydrogenase activity and have reduced levels of long-chain enoyl-CoA hydratase activity. The mutant has fewer but larger peroxisomes.
Orthologs
Proteins
fatty acid beta-oxidation multifunctional protein MFP2 | |
---|---|
Refseq ID | NP_187342 |
Protein GI | 15231317 |
UniProt ID | Q9ZPI5 |
mRNA ID | NM_111566 |
Length | 725 |
RefSeq Status | REVIEWED |
MDSRTKGKTVMEVGGDGVAVITLINPPVNSLSFDVLYNLKSNYEEALSRNDVKAIVITGAKGRFSGGFDISGFGEMQKGNVKEPKAGYISIDIITDLLEAARKPSVAAIDGLALGGGLELAMACHARISAPAAQLGLPELQLGVIPGFGGTQRLPRLVGLTKALEMILTSKPVKAEEGHSLGLIDAVVPPAELVTTARRWALDIVGRRKPWVSSVSKTDKLPPLGEAREILTFAKAQTLKRAPNMKHPLMCLDAIEVGIVSGPRAGLEKEAEVASQVVKLDTTKGLIHVFFSQRGTAKVPGVTDRGLVPRKIKKVAIIGGGLMGSGIATALILSNYPVILKEVNEKFLEAGIGRVKANLQSRVRKGSMSQEKFEKTMSLLKGSLDYESFRDVDMVIEAVIENISLKQQIFADLEKYCPQHCILASNTSTIDLNKIGERTKSQDRIVGAHFFSPAHIMPLLEIVRTNHTSAQVIVDLLDVGKKIKKTPVVVGNCTGFAVNRMFFPYTQAAMFLVECGADPYLIDRAISKFGMPMGPFRLCDLVGFGVAIATATQFIENFSERTYKSMIIPLMQEDKRAGEATRKGFYLYDDKRKAKPDPELKKYIEKARSISGVKLDPKLANLSEKDIIEMTFFPVVNEACRVFAEGIAVKAADLDIAGIMGMGFPPYRGGIMFWADSIGSKYIYSRLDEWSKAYGEFFKPCAFLAERGSKGVLLSAPVKQASSRL |
Gene Information
Entrez Gene ID
Gene Name
fatty acid beta-oxidation multifunctional protein MFP2
Gene Symbol
Species
Arabidopsis thaliana
Gene Ontology (GO Annotations)
GO ID | Source | Type | Description |
---|---|---|---|
GO:0005618 | IDA:TAIR | C | cell wall |
GO:0009514 | IEA:UniProtKB-KW | C | glyoxysome |
GO:0005730 | IDA:TAIR | C | nucleolus |
GO:0005777 | IDA:TAIR | C | peroxisome |
GO:0009506 | IDA:TAIR | C | plasmodesma |
GO:0003857 | IDA:UniProtKB | F | 3-hydroxyacyl-CoA dehydrogenase activity |
GO:0008692 | IEA:UniProtKB-EC | F | 3-hydroxybutyryl-CoA epimerase activity |
GO:0050662 | IEA:InterPro | F | coenzyme binding |
GO:0004165 | IEA:UniProtKB-EC | F | dodecenoyl-CoA delta-isomerase activity |
GO:0004300 | IEA:UniProtKB-EC | F | enoyl-CoA hydratase activity |
GO:0016508 | IDA:UniProtKB | F | long-chain-enoyl-CoA hydratase activity |
GO:0006635 | IEP:TAIR | P | fatty acid beta-oxidation |
KEGG Pathway Links
KEGG Pathway ID | Description |
---|---|
ath00592 | alpha-Linolenic acid metabolism |
ath_M00087 | beta-Oxidation |
ath00071 | Fatty acid degradation |
ath_M00113 | Jasmonic acid biosynthesis |
Domain Information
InterPro Annotations
Accession | Description |
---|---|
IPR006180 | 3-hydroxyacyl-CoA dehydrogenase, conserved site |
IPR006108 | 3-hydroxyacyl-CoA dehydrogenase, C-terminal |
IPR006176 | 3-hydroxyacyl-CoA dehydrogenase, NAD binding |
IPR008927 | 6-phosphogluconate dehydrogenase, C-terminal-like |
IPR029045 | ClpP/crotonase-like domain |
IPR001753 | Crotonase_core_superfam |
IPR013328 | Dehydrogenase, multihelical |
IPR018376 | Enoyl-CoA hydratase/isomerase, conserved site |
IPR016040 | NAD(P)-binding domain |
UniProt Annotations
Entry Information
Gene Name
fatty acid beta-oxidation multifunctional protein MFP2
Protein Entry
MFP2_ARATH
UniProt ID
Species
Arabidopsis
Comments
Comment Type | Description |
---|---|
Biophysicochemical Properties | Kinetic parameters: KM=240 uM for crotonyl-CoA {ECO:0000269|PubMed:10521521}; |
Catalytic Activity | (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl- CoA + H(2)O. |
Catalytic Activity | (3Z)-dodec-3-enoyl-CoA = (2E)-dodec-2-enoyl- CoA. |
Catalytic Activity | (S)-3-hydroxyacyl-CoA + NAD(+) = 3-oxoacyl-CoA + NADH. |
Catalytic Activity | (S)-3-hydroxybutanoyl-CoA = (R)-3- hydroxybutanoyl-CoA. |
Developmental Stage | Expression increases rapidly during seed germination to reach a peak at 2-3 days after imbibition (DAI) and then declines to basal levels at 5 DAI. {ECO:0000269|PubMed:10816107}. |
Disruption Phenotype | Accumulation of long-chain acyl-CoA substrates and increased size of peroxisomes. {ECO:0000269|PubMed:16507084}. |
Domain | The epimerase and isomerase activities are contained in the N-terminal region while the dehydrogenase activity is in the C-terminal region. {ECO:0000250}. |
Function | Involved in peroxisomal fatty acid beta-oxidation during seed germination. Possesses enoyl-CoA hydratase activity against long chain substrates (C14-C18) and 3-hydroxyacyl-CoA dehydrogenase activity against chains of variable sizes (C6-C18). {ECO:0000269|PubMed:10521521, ECO:0000269|PubMed:16507084, ECO:0000269|PubMed:20463021}. |
Pathway | Lipid metabolism; fatty acid beta-oxidation. |
Similarity | In the central section; belongs to the 3-hydroxyacyl- CoA dehydrogenase family. {ECO:0000305}. |
Similarity | In the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family. {ECO:0000305}. |
Subcellular Location | Glyoxysome {ECO:0000305}. Peroxisome {ECO:0000305}. |
Tissue Specificity | Highly expressed in senescing leaves and at lower levels in flowers and siliques. {ECO:0000269|PubMed:10816107}. |
Identical and Related Proteins
Unique RefSeq proteins for LMP010758 (as displayed in Record Overview)
Protein GI | Database | Accession | Length | Protein Name |
---|---|---|---|---|
15231317 | RefSeq | NP_187342 | 725 | fatty acid beta-oxidation multifunctional protein MFP2 |
Identical Sequences to LMP010758 proteins
Reference | Database | Accession | Length | Protein Name |
---|---|---|---|---|
GI:15231317 | EMBL | CBG04538.1 | 725 | unnamed protein product [Arabidopsis thaliana] |
GI:15231317 | EMBL | CBV18060.1 | 725 | unnamed protein product [Arabidopsis thaliana] |
GI:15231317 | GenBank | ACW88301.1 | 725 | Sequence 5318 from patent US 7569389 |
GI:15231317 | GenBank | AEE74468.1 | 725 | fatty acid beta-oxidation multifunctional protein MFP2 [Arabidopsis thaliana] |
GI:15231317 | GenBank | AGX95945.1 | 725 | Sequence 72150 from patent US 8541208 |
GI:15231317 | PDB | 2WTB | 725 | Chain A, Arabidopsis Thaliana Multifuctional Protein, Mfp2 |
Related Sequences to LMP010758 proteins
Reference | Database | Accession | Length | Protein Name |
---|---|---|---|---|
GI:15231317 | GenBank | EOA29963.1 | 725 | hypothetical protein CARUB_v10013066mg [Capsella rubella] |
GI:15231317 | RefSeq | XP_006297065.1 | 725 | hypothetical protein CARUB_v10013066mg [Capsella rubella] |
GI:15231317 | RefSeq | XP_006407895.1 | 725 | hypothetical protein EUTSA_v10020152mg [Eutrema salsugineum] |
GI:15231317 | RefSeq | XP_010445530.1 | 725 | PREDICTED: peroxisomal fatty acid beta-oxidation multifunctional protein MFP2-like isoform X3 [Camelina sativa] |
GI:15231317 | RefSeq | XP_010464276.1 | 725 | PREDICTED: peroxisomal fatty acid beta-oxidation multifunctional protein MFP2 [Camelina sativa] |
GI:15231317 | RefSeq | XP_010486216.1 | 725 | PREDICTED: peroxisomal fatty acid beta-oxidation multifunctional protein MFP2-like [Camelina sativa] |