Gene/Proteome Database (LMPD)
LMPD ID
LMP010824
Gene ID
Species
Arabidopsis thaliana (Arabidopsis)
Gene Name
Myotubularin 1
Gene Symbol
Alternate Names
Myotubularin 1
Chromosome
3
EC Number
3.1.3.95
Summary
Has 3'-phosphatase activity against both phosphatidylinositol-3,5-bisphosphate (PtdIns3,5P2) and Phosphatidylinositol-3-phosphate (PtdIns3P). The in vitro activity was higher with PtdIns3,5P2 than with PtdIns3P.
Orthologs
Proteins
| Myotubularin 1 | |
|---|---|
| Refseq ID | NP_187666 |
| Protein GI | 240255320 |
| UniProt ID | F4J3T8 |
| mRNA ID | NM_111891 |
| Length | 840 |
| RefSeq Status | REVIEWED |
| MTPPRPPSGRVRSLRDYSSESEKMDGTGSWDTLEWTKLDSTSGSGSFSNLSCLLESERVIVEGYGVVLINTDEAGTLLVTNFRILFLSEGTRKVIPLGTIPLATIEKFNKMVLKVQSSPRQSDKIPPRRLLQVTGKDMRIIVYGFRPRTKQRRNVFDALLKCTKPERVWDLYTFACGPSKFGNANPKERLLNEYFRLLGKSSIRASMDMIEDGAFTLSNELWRISDLNSNYNLCQTYPFAFMIPKSISDAELLQACSFRARCRLPVITWCQPGSGAVIARSSQPLVGLMMNMRSNLDEKLVAAFCSQLPGAKGERRKLYIADARPRKNALANGAMGGGSESSSNYFQSEIVFFGIDNIHAMRESFSRVRDYLDMHGTTSSDGRSSFLRHGGWTWGGGNLSSMSASVSLLGDSGWLIHIQSVLAGAAWIAARVAMESASVLVHCSDGWDRTTQLVSLACLLLDPYYRTFAGFQALVEKDWLAFGHPFSDRVGMPNISGSGNFDFPRQSSSAGSFPSSPVRQSSGSAASQSSSSSHGHNNYSPIFMQWIDSVSQLMRMYPCAFEFSPTFLVDFMDCLLSCRFGNFLCNSEKEREQCGIADACGCLWAYLTDLRSFSATSHVHCNPFYDPLKYDGPLLPPAASLAPTLWPQFHLRWACPEEAKAADIGVQCRAMTVKYSEMQKEKEAAERRVDEISFAMESLSAELLRERHLSWVARESANRATKEYAALTRAVQSLGCKINFTTSDVEDDPRSSLENNPRRRNRHGNNSDVSVSISLMPEENTSGNPKGRVCEALCPLRTREGVCRWPEVGCAHVGSQFVGLKANFDAFDRLAIYDSYFQPK | |
Gene Information
Gene Ontology (GO Annotations)
| GO ID | Source | Type | Description |
|---|---|---|---|
| GO:0031410 | IDA:UniProtKB | C | cytoplasmic vesicle |
| GO:0005768 | IEA:UniProtKB-KW | C | endosome |
| GO:0016020 | IEA:UniProtKB-KW | C | membrane |
| GO:0052629 | IDA:UniProtKB | F | phosphatidylinositol-3,5-bisphosphate 3-phosphatase activity |
| GO:0004438 | IDA:UniProtKB | F | phosphatidylinositol-3-phosphatase activity |
| GO:0004725 | IEA:InterPro | F | protein tyrosine phosphatase activity |
| GO:0042631 | IEP:UniProtKB | P | cellular response to water deprivation |
| GO:0035556 | IMP:UniProtKB | P | intracellular signal transduction |
| GO:2000070 | IMP:UniProtKB | P | regulation of response to water deprivation |
REACTOME Pathway Links
Domain Information
UniProt Annotations
Entry Information
Comments
| Comment Type | Description |
|---|---|
| Alternative Products | Event=Alternative splicing; Named isoforms=2; Name=1; IsoId=F4J3T8-1; Sequence=Displayed; Note=Derived from EST data. No experimental confirmation available.; Name=2; IsoId=F4J3T8-2; Sequence=VSP_053569, VSP_053570; |
| Biophysicochemical Properties | Kinetic parameters: KM=201.7 uM for PtdIns3P {ECO:0000269|PubMed:19901554, ECO:0000269|PubMed:22324391}; KM=146 uM for PtdIns3,5P(2) {ECO:0000269|PubMed:19901554, ECO:0000269|PubMed:22324391}; Vmax=94.3 pmol/min/mg enzyme with PtdIns3P as substrate {ECO:0000269|PubMed:19901554, ECO:0000269|PubMed:22324391}; Vmax=142.6 pmol/min/mg enzyme with PtdIns3,5P(2) as substrate {ECO:0000269|PubMed:19901554, ECO:0000269|PubMed:22324391}; |
| Catalytic Activity | 1-phosphatidyl-1D-myo-inositol 3,5- bisphosphate + H(2)O = 1-phosphatidyl-1D-myo-inositol 5-phosphate + phosphate. {ECO:0000269|PubMed:19901554, ECO:0000269|PubMed:22324391, ECO:0000305|PubMed:20967218}. |
| Catalytic Activity | 1-phosphatidyl-1D-myo-inositol 3-phosphate + H(2)O = 1-phosphatidyl-1D-myo-inositol + phosphate. {ECO:0000269|PubMed:19901554, ECO:0000269|PubMed:20967218, ECO:0000269|PubMed:22324391}. |
| Developmental Stage | Expressed in young seedlings, especially at the tip of the growing shoot meristems. Later observed in roots and in aerial parts. Weakly expressed in leaves with local higher levels in the trichomes, hydathodes and in cotyledon veins. Present at low levels in flowers with higher accumulation in cells at organ-stem junctions, as well as in the septum and the funiculi of the developing siliques. Also observed in a diffuse pattern, appearing as patches along the stem but also concentrated at the peduncle. {ECO:0000269|PubMed:22324391}. |
| Disruption Phenotype | No visible phenotype in normal conditions. Impaired accumulation of PtdIns5P in response to stress such as dehydration, leading to an increased resistance. {ECO:0000269|PubMed:20967218, ECO:0000269|PubMed:22324391}. |
| Function | Phosphatase with phosphoinositide 3'-phosphatase activity that can use phosphatidylinositol-3-phosphate (PtdIns3P) and phosphatidylinositol-3,5-diphosphate (PtdIns3,5P(2)) as substrates and produces phosphatidylinositol-5-phosphate (PtdIns5P); participates in pathway(s) that transfer gene regulatory signals to the nucleus. Required for recovery after water deprivation, via the accumulation of PtdIns5P upon dehydration; high PtdIns5P levels mediate ATX1 cytoplasmic localization, thus down-regulating the expression of ATX1- dependent genes. Confers sensitivity to soil-water-deficit stress. {ECO:0000269|PubMed:19901554, ECO:0000269|PubMed:20967218, ECO:0000269|PubMed:22324391}. |
| Induction | Accumulates upon dehydration. {ECO:0000269|PubMed:19901554}. |
| Sequence Caution | Sequence=AAF76357.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305}; Sequence=AAG51396.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305}; |
| Similarity | Belongs to the protein-tyrosine phosphatase family. Non-receptor class myotubularin subfamily. {ECO:0000305}. |
| Similarity | Contains 1 GRAM domain. {ECO:0000305}. |
| Similarity | Contains 1 myotubularin phosphatase domain. {ECO:0000255|PROSITE-ProRule:PRU00669}. |
| Subcellular Location | Cytoplasm {ECO:0000269|PubMed:22324391}. Endosome membrane {ECO:0000269|PubMed:22324391}; Peripheral membrane protein {ECO:0000269|PubMed:22324391}. Note=Present in granular particles of varying abundance and size at the cell periphery and throughout the cytoplasm. |
| Tissue Specificity | Mostly expressed in siliques and leaves (including hydathodes), and, to a lower extent, in flowers and roots. {ECO:0000269|PubMed:22324391}. |
Identical and Related Proteins
Unique RefSeq proteins for LMP010824 (as displayed in Record Overview)
Identical Sequences to LMP010824 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:240255320 | GenBank | AEE74924.1 | 840 | Myotubularin 1 [Arabidopsis thaliana] |
| GI:240255320 | SwissProt | F4J3T8.1 | 840 | RecName: Full=Phosphatidylinositol-3-phosphatase myotubularin-1; Short=AtMTM1; AltName: Full=Phosphatidylinositol-3,5-bisphosphate 3-phosphatase; AltName: Full=Phosphatidylinositol-3-phosphate phosphatase [Arabidopsis thaliana] |
Related Sequences to LMP010824 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:240255320 | GenBank | AAF76357.1 | 840 | myotubularin related protein, putative [Arabidopsis thaliana] |
| GI:240255320 | GenBank | ESQ48998.1 | 841 | hypothetical protein EUTSA_v10020062mg [Eutrema salsugineum] |
| GI:240255320 | RefSeq | XP_002884798.1 | 828 | hypothetical protein ARALYDRAFT_317855 [Arabidopsis lyrata subsp. lyrata] |
| GI:240255320 | RefSeq | XP_006407545.1 | 841 | hypothetical protein EUTSA_v10020062mg [Eutrema salsugineum] |
| GI:240255320 | RefSeq | XP_010485735.1 | 843 | PREDICTED: LOW QUALITY PROTEIN: phosphatidylinositol-3-phosphatase myotubularin-1-like [Camelina sativa] |
| GI:240255320 | RefSeq | XP_010464732.1 | 843 | PREDICTED: phosphatidylinositol-3-phosphatase myotubularin-1-like [Camelina sativa] |