Gene/Proteome Database (LMPD)
LMPD ID
LMP010866
Gene ID
Species
Arabidopsis thaliana (Arabidopsis)
Gene Name
phospholipase A2-alpha
Gene Symbol
Synonyms
ATSPLA2-ALPHA; PHOSPHOLIPASE A2-ALPHA; PLA2-ALPHA
Alternate Names
phospholipase A2-alpha
Chromosome
2
EC Number
3.1.1.4
Summary
Encodes a secretory phospholipase A2 enzyme, which specifically hydrolyzes the sn-2 position of phospholipids. The enzyme has a preference towards linoleoyl acyl chain over palmitoyl acyl chain. It also has a slight preference for phosphatidylcholine over phosphatidylethanolamine.
Orthologs
Proteins
Gene Information
Entrez Gene ID
Gene Name
phospholipase A2-alpha
Gene Symbol
Species
Arabidopsis thaliana
Gene Ontology (GO Annotations)
| GO ID | Source | Type | Description |
|---|---|---|---|
| GO:0005794 | IDA:UniProtKB | C | Golgi apparatus |
| GO:0005576 | IEA:UniProtKB-KW | C | extracellular region |
| GO:0005773 | TAS:TAIR | C | vacuole |
| GO:0005509 | IEA:InterPro | F | calcium ion binding |
| GO:0004623 | IDA:UniProtKB | F | phospholipase A2 activity |
| GO:0016042 | IEA:UniProtKB-KW | P | lipid catabolic process |
| GO:0006644 | IEA:InterPro | P | phospholipid metabolic process |
KEGG Pathway Links
Domain Information
UniProt Annotations
Entry Information
Comments
| Comment Type | Description |
|---|---|
| Biophysicochemical Properties | Kinetic parameters: Vmax=15.3 umol/min/mg enzyme toward Dioleolyl- phosphoethanolamine (in the presence of 10 mM Calcium) {ECO:0000269|PubMed:17692835}; Vmax=9.9 umol/min/mg enzyme toward DOPG (in the presence of 10 mM Calcium) {ECO:0000269|PubMed:17692835}; Vmax=16.7 umol/min/mg enzyme toward Dioleolyl-phosphocholine (in the presence of 10 mM Calcium) {ECO:0000269|PubMed:17692835}; Vmax=19.5 umol/min/mg enzyme toward Dilinoleoyl-phosphocholine (in the presence of 10 mM Calcium) {ECO:0000269|PubMed:17692835}; Vmax=20.6 umol/min/mg enzyme toward Dipalmitoyl-phosphocholine (in the presence of 10 mM Calcium) {ECO:0000269|PubMed:17692835}; Vmax=27.3 umol/min/mg enzyme toward Dimyristoyl-phosphocholine (in the presence of 10 mM Calcium) {ECO:0000269|PubMed:17692835}; Vmax=29.3 umol/min/mg enzyme toward Dilauroyl-phosphocholine (in the presence of 10 mM Calcium) {ECO:0000269|PubMed:17692835}; Vmax=35.5 umol/min/mg enzyme toward Didecanoyl-phosphocholine (in the presence of 10 mM Calcium) {ECO:0000269|PubMed:17692835}; Vmax=14.4 umol/min/mg enzyme toward Dioctanoyl-phosphocholine (in the presence of 10 mM Calcium) {ECO:0000269|PubMed:17692835}; Vmax=5.9 umol/min/mg enzyme toward Diheptanoyl-phosphocholine (in the presence of 10 mM Calcium) {ECO:0000269|PubMed:17692835}; Vmax=1.1 umol/min/mg enzyme toward Dihexanoyl-phosphocholine (in the presence of 10 mM Calcium) {ECO:0000269|PubMed:17692835}; pH dependence: Optimum pH is 8.5-9. {ECO:0000269|PubMed:17692835}; Temperature dependence: Optimum temperature is 30-40 degrees Celsius. {ECO:0000269|PubMed:17692835}; |
| Catalytic Activity | Phosphatidylcholine + H(2)O = 1- acylglycerophosphocholine + a carboxylate. {ECO:0000255|PROSITE- ProRule:PRU10035, ECO:0000269|PubMed:16140037}. |
| Cofactor | Name=Ca(2+); Xref=ChEBI:CHEBI:29108; Note=Binds 1 Ca(2+) ion per subunit.; |
| Function | PA2 catalyzes the calcium-dependent hydrolysis of the 2- acyl groups in 3-sn-phosphoglycerides. Releases lysophospholipids (LPLs) and free fatty acids (FFAs) from membrane phospholipids in response to hormones and other external stimuli. Modulates the trafficking of PIN proteins to the plasma membrane. {ECO:0000269|PubMed:16140037, ECO:0000269|PubMed:20525850}. |
| Miscellaneous | The enzyme has a preference towards linoleoyl acyl chain over palmitoyl acyl chain. It also has a slight preference for phosphatidylethanolamine over phosphatidylcholine. |
| Similarity | Belongs to the phospholipase A2 family. {ECO:0000305}. |
| Subcellular Location | Secreted {ECO:0000250}. Golgi apparatus {ECO:0000269|PubMed:20525850}. |
| Tissue Specificity | Ubiquitous but expressed at a low level. {ECO:0000269|PubMed:15748654, ECO:0000269|PubMed:16140037}. |
Identical and Related Proteins
Unique RefSeq proteins for LMP010866 (as displayed in Record Overview)
| Protein GI | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| 18396422 | RefSeq | NP_565337 | 148 | phospholipase A2-alpha |
Identical Sequences to LMP010866 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:18396422 | DBBJ | BAD95375.1 | 148 | hypothetical protein [Arabidopsis thaliana] |
| GI:18396422 | GenBank | AAN72211.1 | 148 | expressed protein [Arabidopsis thaliana] |
| GI:18396422 | GenBank | AAR04682.1 | 148 | phospholipase A2 alpha [Arabidopsis thaliana] |
| GI:18396422 | GenBank | AEC06025.1 | 148 | phospholipase A2-alpha [Arabidopsis thaliana] |
| GI:18396422 | GenBank | AFN93898.1 | 148 | Sequence 2 from patent US 8203031 |
| GI:18396422 | SwissProt | Q8S8N6.1 | 148 | RecName: Full=Phospholipase A2-alpha; AltName: Full=Secretory phospholipase A2-alpha; Short=AtsPLA2-alpha; Flags: Precursor [Arabidopsis thaliana] |
Related Sequences to LMP010866 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:18396422 | GenBank | EFH60001.1 | 148 | phospholipase A2-alpha [Arabidopsis lyrata subsp. lyrata] |
| GI:18396422 | RefSeq | XP_002883742.1 | 148 | phospholipase A2-alpha [Arabidopsis lyrata subsp. lyrata] |
| GI:18396422 | RefSeq | XP_006298748.1 | 148 | hypothetical protein CARUB_v10014847mg [Capsella rubella] |
| GI:18396422 | RefSeq | XP_010412986.1 | 192 | PREDICTED: phospholipase A2-alpha [Camelina sativa] |
| GI:18396422 | RefSeq | XP_010467129.1 | 192 | PREDICTED: phospholipase A2-alpha [Camelina sativa] |
| GI:18396422 | RefSeq | XP_010488801.1 | 192 | PREDICTED: phospholipase A2-alpha-like [Camelina sativa] |