Gene/Proteome Database (LMPD)
LMPD ID
LMP010895
Gene ID
Species
Arabidopsis thaliana (Arabidopsis)
Gene Name
phospholipid:diacylglycerol acyltransferase
Gene Symbol
Synonyms
ARABIDOPSIS THALIANA PHOSPHOLIPID:DIACYLGLYCEROL ACYLTRANSFERASE; ATPDAT; PDAT; PDAT1; PHOSPHOLIPID DIACYLGLYCEROL ACYLTRANSFERASE; phospholipid:diacylglycerol acyltransferase; PHOSPHOLIPID:DIACYLGLYCEROL ACYLTRANSFERASE 1; T6I14.2
Alternate Names
phospholipid:diacylglycerol acyltransferase
Chromosome
5
EC Number
2.3.1.158
Proteins
| phospholipid:diacylglycerol acyltransferase | |
|---|---|
| Refseq ID | NP_196868 |
| Protein GI | 15240676 |
| UniProt ID | Q9FNA9 |
| mRNA ID | NM_121367 |
| Length | 671 |
| RefSeq Status | REVIEWED |
| MPLIHRKKPTEKPSTPPSEEVVHDEDSQKKPHESSKSHHKKSNGGGKWSCIDSCCWFIGCVCVTWWFLLFLYNAMPASFPQYVTERITGPLPDPPGVKLKKEGLKAKHPVVFIPGIVTGGLELWEGKQCADGLFRKRLWGGTFGEVYKRPLCWVEHMSLDNETGLDPAGIRVRAVSGLVAADYFAPGYFVWAVLIANLAHIGYEEKNMYMAAYDWRLSFQNTEVRDQTLSRMKSNIELMVSTNGGKKAVIVPHSMGVLYFLHFMKWVEAPAPLGGGGGPDWCAKYIKAVMNIGGPFLGVPKAVAGLFSAEAKDVAVARAIAPGFLDTDIFRLQTLQHVMRMTRTWDSTMSMLPKGGDTIWGGLDWSPEKGHTCCGKKQKNNETCGEAGENGVSKKSPVNYGRMISFGKEVAEAAPSEINNIDFRGAVKGQSIPNHTCRDVWTEYHDMGIAGIKAIAEYKVYTAGEAIDLLHYVAPKMMARGAAHFSYGIADDLDDTKYQDPKYWSNPLETKLPNAPEMEIYSLYGVGIPTERAYVYKLNQSPDSCIPFQIFTSAHEEDEDSCLKAGVYNVDGDETVPVLSAGYMCAKAWRGKTRFNPSGIKTYIREYNHSPPANLLEGRGTQSGAHVDIMGNFALIEDIMRVAAGGNGSDIGHDQVHSGIFEWSERIDLKL | |
Gene Information
Entrez Gene ID
Gene Name
phospholipid:diacylglycerol acyltransferase
Gene Symbol
Species
Arabidopsis thaliana
Gene Ontology (GO Annotations)
| GO ID | Source | Type | Description |
|---|---|---|---|
| GO:0005783 | IDA:TAIR | C | endoplasmic reticulum |
| GO:0016021 | IEA:UniProtKB-KW | C | integral component of membrane |
| GO:0005773 | IDA:TAIR | C | vacuole |
| GO:0008374 | IEA:InterPro | F | O-acyltransferase activity |
| GO:0046027 | IDA:TAIR | F | phospholipid:diacylglycerol acyltransferase activity |
| GO:0006071 | IEA:UniProtKB-KW | P | glycerol metabolic process |
| GO:0019432 | IGI:TAIR | P | triglyceride biosynthetic process |
KEGG Pathway Links
| KEGG Pathway ID | Description |
|---|---|
| ath00561 | Glycerolipid metabolism |
BIOCYC Pathway Links
| BIOCYC Pathway ID | Description |
|---|---|
| TRIGLSYN-PWY | triacylglycerol biosynthesis |
| TRIGLSYN-PWY | triacylglycerol biosynthesis |
Domain Information
UniProt Annotations
Entry Information
Gene Name
phospholipid:diacylglycerol acyltransferase
Protein Entry
PDAT1_ARATH
UniProt ID
Species
Arabidopsis
Comments
| Comment Type | Description |
|---|---|
| Catalytic Activity | Phospholipid + 1,2-diacylglycerol = lysophospholipid + triacylglycerol. |
| Developmental Stage | Peak of expression in seeds 9 days after flowering. {ECO:0000269|PubMed:20101470}. |
| Disruption Phenotype | No visible phenotype. No changes in the fatty acid content and composition of seeds. Dgat1 and pdat1 double mutants produce sterile deformed pollen. {ECO:0000269|PubMed:15907694, ECO:0000269|PubMed:20040537}. |
| Function | Triacylglycerol formation by an acyl-CoA independent pathway. The enzyme preferentially transfers acyl groups from the sn-2 position of a phospholipid to diacylglycerol, thus forming an sn-1-lysophospholipid. Involved in epoxy and hydroxy fatty acid accumulation in seeds. Has complementary functions with DAG1 that are essential for triacylglycerol synthesis and normal development of both seeds and pollen. {ECO:0000269|PubMed:15247387, ECO:0000269|PubMed:20040537, ECO:0000269|PubMed:20101470}. |
| Pathway | Glycerolipid metabolism; triacylglycerol biosynthesis. |
| Sequence Caution | Sequence=AAO17787.1; Type=Erroneous initiation; Note=Translation N-terminally extended.; Evidence={ECO:0000305}; Sequence=AAO17787.1; Type=Frameshift; Positions=100; Evidence={ECO:0000305}; |
| Similarity | Belongs to the AB hydrolase superfamily. Lipase family. {ECO:0000305}. |
| Subcellular Location | Membrane {ECO:0000305}; Single-pass membrane protein {ECO:0000305}. |
| Tissue Specificity | Ubiquitous. Highest expression in young developing seeds. {ECO:0000269|PubMed:15247387, ECO:0000269|PubMed:20040537, ECO:0000269|PubMed:20101470}. |
Identical and Related Proteins
Unique RefSeq proteins for LMP010895 (as displayed in Record Overview)
| Protein GI | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| 15240676 | RefSeq | NP_196868 | 671 | phospholipid:diacylglycerol acyltransferase |
Identical Sequences to LMP010895 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:15240676 | GenBank | ACP74342.1 | 671 | Sequence 5 from patent US 7498026 |
| GI:15240676 | GenBank | ACP75789.1 | 671 | Sequence 30 from patent US 7498483 |
| GI:15240676 | GenBank | ACS07585.1 | 671 | Sequence 30 from patent US 7534935 |
| GI:15240676 | GenBank | ADA08787.1 | 671 | Sequence 74 from patent US 7601890 |
| GI:15240676 | GenBank | ADC16983.1 | 671 | Sequence 6 from patent US 7635582 |
| GI:15240676 | gnl | TAIR | 671 | phospholipid:diacylglycerol acyltransferase [Arabidopsis thaliana] |
Related Sequences to LMP010895 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:15240676 | GenBank | EFH47836.1 | 672 | predicted protein [Arabidopsis lyrata subsp. lyrata] |
| GI:15240676 | RefSeq | XP_002871577.1 | 672 | predicted protein [Arabidopsis lyrata subsp. lyrata] |
| GI:15240676 | RefSeq | XP_006286627.1 | 666 | hypothetical protein CARUB_v10002446mg [Capsella rubella] |
| GI:15240676 | RefSeq | XP_010453452.1 | 671 | PREDICTED: phospholipid:diacylglycerol acyltransferase 1-like [Camelina sativa] |
| GI:15240676 | RefSeq | XP_010492131.1 | 671 | PREDICTED: phospholipid:diacylglycerol acyltransferase 1-like isoform X1 [Camelina sativa] |
| GI:15240676 | RefSeq | XP_010492132.1 | 671 | PREDICTED: phospholipid:diacylglycerol acyltransferase 1-like isoform X2 [Camelina sativa] |