Gene/Proteome Database (LMPD)

LMPD ID
LMP010899
Gene ID
842275
Species
Arabidopsis thaliana (Arabidopsis)
Gene Name
phospholipid-transporting ATPase 3
Gene Symbol
ALA3
Synonyms
aminophospholipid ATPase 3; F23H11.14; F23H11_14
Alternate Names
phospholipid-transporting ATPase 3
Chromosome
1
EC Number
3.6.3.1
Summary
Encodes a phospholipid translocase. Involved in secretory vesicle formation from trans-Golgi in peripheral columella cells at the root tip. Mutants have short primary roots and grow slower.
Orthologs
View orthologs and multiple alignments for ALA3

Proteins

phospholipid-transporting ATPase 3
Refseq ID NP_176191
Protein GI 15218927
UniProt ID Q9XIE6
mRNA ID NM_104675
Length 1213
RefSeq Status REVIEWED
MVRSGSFSVDSSATHQRTPSRTVTLGHIQPQAPTYRTVYCNDRESNQPVRFKGNSISTTKYNVFTFLPKGLFEQFRRIANIYFLGISCLSMTPISPVSPITNVAPLSMVLLVSLIKEAFEDWKRFQNDMSINNSTVEILQDQQWVSIPWRKLQVGDIVKIKKDGFFPADILFMSSTNSDGICYVETANLDGETNLKIRKALERTWDYLVPEKAYEFKGEIQCEQPNNSLYTFTGNLVVQKQTLPLSPDQLLLRGCSLRNTEYIVGAVVFTGHETKVMMNAMNAPSKRSTLEKKLDKLIITIFCVLVTMCLIGAIGCSIVTDREDKYLGLHNSDWEYRNGLMIGFFTFFTLVTLFSSIIPISLYVSIEMIKFIQSTQFINRDLNMYHAETNTPASARTSNLNEELGQVEYIFSDKTGTLTRNLMEFFKCSIGGVSYGCGVTEIEKGIAQRHGLKVQEEQRSTGAIREKGFNFDDPRLMRGAWRNEPNPDLCKELFRCLAICHTVLPEGDESPEKIVYQAASPDEAALVTAAKNFGFFFYRRTPTMVYVRESHVEKMGKIQDVAYEILNVLEFNSTRKRQSVVCRFPDGRLVLYCKGADNVIFERLANGMDDVRKVTREHLEHFGSSGLRTLCLAYKDLNPETYDSWNEKFIQAKSALRDREKKLDEVAELIEKDLILIGSTAIEDKLQEGVPTCIETLSRAGIKIWVLTGDKMETAINIAYACNLINNEMKQFVISSETDAIREAEERGDQVEIARVIKEEVKRELKKSLEEAQHSLHTVAGPKLSLVIDGKCLMYALDPSLRVMLLSLSLNCTSVVCCRVSPLQKAQVTSLVRKGAQKITLSIGDGANDVSMIQAAHVGIGISGMEGMQAVMASDFAIAQFRFLTDLLLVHGRWSYLRICKVVMYFFYKNLTFTLTQFWFTFRTGFSGQRFYDDWFQSLFNVVFTALPVIVLGLFEKDVSASLSKRYPELYREGIRNSFFKWRVVAVWATSAVYQSLVCYLFVTTSSFGAVNSSGKVFGLWDVSTMVFTCLVIAVNVRILLMSNSITRWHYITVGGSILAWLVFAFVYCGIMTPHDRNENVYFVIYVLMSTFYFYFTLLLVPIVSLLGDFIFQGVERWFFPYDYQIVQEIHRHESDASKADQLEVENELTPQEARSYAISQLPRELSKHTGFAFDSPGYESFFASQLGIYAPQKAWDVARRASMRSRPKVPKK

Gene Information

Entrez Gene ID
842275
Gene Name
phospholipid-transporting ATPase 3
Gene Symbol
ALA3
Species
Arabidopsis thaliana

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005794 IDA:TAIR C Golgi apparatus
GO:0000139 IMP:GOC C Golgi membrane
GO:0005783 IEA:UniProtKB-KW C endoplasmic reticulum
GO:0005768 IDA:TAIR C endosome
GO:0016021 IEA:UniProtKB-KW C integral component of membrane
GO:0005886 IDA:TAIR C plasma membrane
GO:0005802 IDA:TAIR C trans-Golgi network
GO:0005524 IEA:UniProtKB-KW F ATP binding
GO:0019829 IEA:InterPro F cation-transporting ATPase activity
GO:0000287 IEA:InterPro F magnesium ion binding
GO:0005548 IDA:TAIR F phospholipid transporter activity
GO:0004012 IEA:UniProtKB-EC F phospholipid-translocating ATPase activity
GO:0048194 IMP:TAIR P Golgi vesicle budding
GO:0048364 IMP:TAIR P root development
GO:0048367 IMP:TAIR P shoot system development

Domain Information

InterPro Annotations

Accession Description
IPR008250 ATPase_P-typ_transduc_dom_A
IPR001757 Cation_transp_P_typ_ATPase
IPR023214 HAD-like domain
IPR023299 P-type ATPase, cytoplasmic domain N
IPR018303 P-type ATPase, phosphorylation site
IPR006539 P-type ATPase, subfamily IV

UniProt Annotations

Entry Information

Gene Name
phospholipid-transporting ATPase 3
Protein Entry
ALA3_ARATH
UniProt ID
Q9XIE6
Species
Arabidopsis

Comments

Comment Type Description
Catalytic Activity ATP + H(2)O + phospholipid(Side 1) = ADP + phosphate + phospholipid(Side 2).
Disruption Phenotype Impaired growth of roots and shoots. Roots devoided of the characteristic trans-Golgi proliferation of slime vesicles containing polysaccharides and enzymes for secretion. Aberrant trichome expansion, reduced primary root growth and longer root hairs. Impaired pollen growth. {ECO:0000269|PubMed:18344284, ECO:0000269|PubMed:19566596}.
Function Involved in transport of phospholipids. Contributes to transmembrane flipping of lipids. Required for secretory processes during plant development. Requires an interaction with an ALIS protein for activity. Has activity with phosphatidylserine, phosphatidylcholine and phosphatidylethanolamine, but not with lysolipid. {ECO:0000269|PubMed:18344284, ECO:0000269|PubMed:19566596, ECO:0000269|PubMed:20053675}.
Miscellaneous The intracellular targeting signals and lipid specificity determinants reside in the catalytic ALA subunit.
Sequence Caution Sequence=AAD39325.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
Similarity Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IV subfamily. {ECO:0000305}.
Subcellular Location Golgi apparatus membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein. Note=Requires the presence of an ALIS protein to exit the endoplasmic reticulum to the Golgi.
Subunit Associates with ALIS1 to form a stable and active complex. Interacts with ALIS3 and ALIS5 in a heterologous system. {ECO:0000269|PubMed:18344284, ECO:0000269|PubMed:20053675}.
Tissue Specificity Expressed in petals and sepals, but not in reproductive tissues. In siliques, detected in the upper part of the seed pod and in the area between the seed pod and the stem, but not in developing seeds. Strong expression in vascular shoot tissues and in stomatal guard cells of young rosettes leaves. In roots, expressed in cells surrounding the xylem and in central and peripheral columella cells. Detected in developing and mature trichomes, roots, pollen and growing pollen tubes. {ECO:0000269|PubMed:18344284, ECO:0000269|PubMed:19566596}.

Identical and Related Proteins

Unique RefSeq proteins for LMP010899 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
15218927 RefSeq NP_176191 1213 phospholipid-transporting ATPase 3

Identical Sequences to LMP010899 proteins

Reference Database Accession Length Protein Name
GI:15218927 GenBank AAM10325.1 1213 At1g59820/F23H11_14 [Arabidopsis thaliana]
GI:15218927 GenBank AEE33621.1 1213 phospholipid-transporting ATPase 3 [Arabidopsis thaliana]
GI:15218927 SwissProt Q9XIE6.2 1213 RecName: Full=Phospholipid-transporting ATPase 3; Short=AtALA3; AltName: Full=Aminophospholipid ATPase 3; AltName: Full=Aminophospholipid flippase 3; AltName: Full=Protein IRREGULAR TRICHOME BRANCH 2 [Arabidopsis thaliana]

Related Sequences to LMP010899 proteins

Reference Database Accession Length Protein Name
GI:15218927 GenBank EFH64429.1 1215 hypothetical protein ARALYDRAFT_475328 [Arabidopsis lyrata subsp. lyrata]
GI:15218927 GenBank EOA33555.1 1214 hypothetical protein CARUB_v10019677mg [Capsella rubella]
GI:15218927 RefSeq XP_002888170.1 1215 hypothetical protein ARALYDRAFT_475328 [Arabidopsis lyrata subsp. lyrata]
GI:15218927 RefSeq XP_006300657.1 1214 hypothetical protein CARUB_v10019677mg [Capsella rubella]
GI:15218927 RefSeq XP_010511001.1 1214 PREDICTED: phospholipid-transporting ATPase 3 isoform X1 [Camelina sativa]
GI:15218927 RefSeq XP_010470182.1 1214 PREDICTED: phospholipid-transporting ATPase 3-like isoform X1 [Camelina sativa]