Gene/Proteome Database (LMPD)

LMPD ID
LMP010921
Gene ID
821870
Species
Arabidopsis thaliana (Arabidopsis)
Gene Name
plastidial pyruvate kinase 1
Gene Symbol
PKP-ALPHA
Synonyms
PKP1; PLASTIDIAL PYRUVATE KINASE 1
Alternate Names
plastidial pyruvate kinase 1
Chromosome
3
EC Number
2.7.1.40
Summary
encodes a chloroplast pyruvate kinase alpha subunit. Important for seed oil biosynthesis. Ubiquitously expressed, with significantly increased expression in maturing seeds.
Orthologs
View orthologs and multiple alignments for PKP-ALPHA

Proteins

plastidial pyruvate kinase 1
Refseq ID NP_566720
Protein GI 18403597
UniProt ID Q9LIK0
mRNA ID NM_113196
Length 596
RefSeq Status REVIEWED
MSQSIQFSTPSHTPHLLHLPHSQFNRPLSSISFRRFPLTTIKYTSIRASSSSSPSPDLDSSSSSSSSQVLLSPNGTGAVKSDERSVVATAVTTDTSGIEVDTVTEAELKENGFRSTRRTKLICTIGPATCGFEQLEALAVGGMNVARLNMCHGTRDWHRGVIRSVRRLNEEKGFAVAIMMDTEGSEIHMGDLGGEASAKAEDGEVWTFTVRAFDSSRPERTISVSYDGFAEDVRVGDELLVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGSLVRERNAMLPTISSKDWLDIDFGIAEGVDFIAVSFVKSAEVINHLKSYLAARSRGGEIGVIAKIESIDSLTNLEEIILASDGAMVARGDLGAQIPLEQVPAAQQRIVQVCRALNKPVIVASQLLESMIEYPTPTRAEVADVSEAVRQRSDALMLSGESAMGQFPDKALTVLRTVSLRIERWWREEKRHESVPLQAIGSSFSDKISEEICNSAAKMANNLGVDAVFVYTTSGHMASLVSRCRPDCPIFAFTTTTSVRRRLNLQWGLIPFRLSFSDDMESNLNKTFSLLKSRGMIKSGDLVIAVSDMLQSIQVMNVP

Gene Information

Entrez Gene ID
821870
Gene Name
plastidial pyruvate kinase 1
Gene Symbol
PKP-ALPHA
Species
Arabidopsis thaliana

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0009507 IDA:TAIR C chloroplast
GO:0009570 IDA:TAIR C chloroplast stroma
GO:0005524 IEA:UniProtKB-KW F ATP binding
GO:0000287 IDA:UniProtKB F magnesium ion binding
GO:0030955 IDA:UniProtKB F potassium ion binding
GO:0004743 IDA:TAIR F pyruvate kinase activity
GO:0006096 IEA:UniProtKB-UniPathway P glycolytic process
GO:0006629 IGI:TAIR P lipid metabolic process
GO:0046686 IEP:TAIR P response to cadmium ion
GO:0010431 IMP:UniProtKB P seed maturation

KEGG Pathway Links

KEGG Pathway ID Description
ath01230 Biosynthesis of amino acids
ath01200 Carbon metabolism
ath00010 Glycolysis / Gluconeogenesis
ath_M00050 Guanine ribonucleotide biosynthesis IMP => GDP,GTP

Domain Information

InterPro Annotations

Accession Description
IPR001697 Pyruvate kinase
IPR015795 Pyruvate kinase, C-terminal
IPR018209 Pyruvate kinase, active site
IPR015794 Pyruvate kinase, alpha/beta
IPR015793 Pyruvate kinase, barrel
IPR015806 Pyruvate kinase, beta-barrel insert domain
IPR011037 Pyruvate kinase-like, insert domain
IPR015813 Pyruvate/Phosphoenolpyruvate kinase-like domain

UniProt Annotations

Entry Information

Gene Name
plastidial pyruvate kinase 1
Protein Entry
PKP1_ARATH
UniProt ID
Q9LIK0
Species
Arabidopsis

Comments

Comment Type Description
Biophysicochemical Properties pH dependence: Optimum pH is 7.8-8.2. {ECO:0000269|PubMed:17557808, ECO:0000269|PubMed:17892448};
Catalytic Activity ATP + pyruvate = ADP + phosphoenolpyruvate.
Cofactor Name=K(+); Xref=ChEBI:CHEBI:29103; Evidence={ECO:0000269|PubMed:17557808};
Cofactor Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000269|PubMed:17557808};
Developmental Stage In seeds, accumulates in endosperm and embryo. In torpedo-shaped embryos, restricted to the hypocotyl and in the outer parts of the young cotyledons. In later embryo stages, present in all tissues except root tips. {ECO:0000269|PubMed:17892448}.
Disruption Phenotype Reduced plastidial pyruvate kinase activity and altered seed oil content leading to wrinkled seeds, retarded embryo elongation and reduced seed germination. {ECO:0000269|PubMed:17892448, ECO:0000269|PubMed:17965177}.
Function Required for plastidial pyruvate kinase activity. Involved in seed oil accumulation, embryo development and seed storage compounds mobilization upon germination. {ECO:0000269|PubMed:17892448, ECO:0000269|PubMed:17965177}.
Induction Induced by cadmium. {ECO:0000269|PubMed:16502469}.
Pathway Carbohydrate degradation; glycolysis; pyruvate from D- glyceraldehyde 3-phosphate: step 5/5.
Similarity Belongs to the pyruvate kinase family. {ECO:0000305}.
Subcellular Location Plastid, chloroplast stroma {ECO:0000269|PubMed:15028209, ECO:0000269|PubMed:16207701, ECO:0000269|PubMed:17557808, ECO:0000269|PubMed:17892448, ECO:0000269|PubMed:18431481, ECO:0000269|PubMed:20061580, ECO:0000269|PubMed:20423899}.
Subunit Oligomer of alpha and beta subunits. {ECO:0000269|PubMed:17557808}.
Tissue Specificity Mostly expressed in seeds, and, to a lower extent, in roots, leaves (veins and trichomes), stems, inflorescences, siliques, pollen (grains and tubes) and flowers (sepals and petals). {ECO:0000269|PubMed:17557808, ECO:0000269|PubMed:17892448}.

Identical and Related Proteins

Unique RefSeq proteins for LMP010921 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
18403597 RefSeq NP_566720 596 plastidial pyruvate kinase 1

Identical Sequences to LMP010921 proteins

Reference Database Accession Length Protein Name
GI:18403597 EMBL CBU91018.1 596 unnamed protein product [Arabidopsis thaliana]
GI:18403597 EMBL CDM63699.1 596 unnamed protein product [Arabidopsis thaliana]
GI:18403597 EMBL CDM63713.1 596 unnamed protein product [Arabidopsis thaliana]
GI:18403597 GenBank AEE76697.1 596 Pyruvate kinase family protein [Arabidopsis thaliana]
GI:18403597 GenBank AGM79980.1 596 Sequence 26 from patent US 8426676
GI:18403597 GenBank AGX58110.1 596 Sequence 16533 from patent US 8541208

Related Sequences to LMP010921 proteins

Reference Database Accession Length Protein Name
GI:18403597 EMBL CBF66089.1 596 unnamed protein product [Arabidopsis thaliana]
GI:18403597 EMBL CBU90962.1 596 unnamed protein product [Arabidopsis thaliana]
GI:18403597 GenBank AAM61702.1 596 pyruvate kinase, putative [Arabidopsis thaliana]
GI:18403597 GenBank EFH59646.1 597 hypothetical protein ARALYDRAFT_479804 [Arabidopsis lyrata subsp. lyrata]
GI:18403597 GenBank AGX58054.1 596 Sequence 16421 from patent US 8541208
GI:18403597 RefSeq XP_002883387.1 597 hypothetical protein ARALYDRAFT_479804 [Arabidopsis lyrata subsp. lyrata]