Gene/Proteome Database (LMPD)

LMPD ID
LMP010922
Gene ID
835369
Species
Arabidopsis thaliana (Arabidopsis)
Gene Name
plastidial pyruvate kinase 2
Gene Symbol
PKP-BETA1
Synonyms
MXC20.15; MXC20_15; PKP-BETA1; PKP1; PKP2; PLASTIDIAL PYRUVATE KINASE 1; PLASTIDIAL PYRUVATE KINASE 2; plastidic pyruvate kinase beta subunit 1
Alternate Names
plastidial pyruvate kinase 2
Chromosome
5
EC Number
2.7.1.40

Proteins

plastidial pyruvate kinase 2
Refseq ID NP_200104
Protein GI 15237303
UniProt ID Q9FLW9
mRNA ID NM_124670
Length 579
RefSeq Status REVIEWED
MAQVVATRSIQGSMLSPNGGSVSTRSEKLLKPASFAVKVLGNEAKRSGRVSVRSRRVVDTTVRSARVETEVIPVSPEDVPNREEQLERLLEMQQFGDTSVGMWSKPTVRRKTKIVCTVGPSTNTREMIWKLAEAGMNVARMNMSHGDHASHKKVIDLVKEYNAQTKDNTIAIMLDTKGPEVRSGDLPQPIMLDPGQEFTFTIERGVSTPSCVSVNYDDFVNDVEAGDMLLVDGGMMSFMVKSKTKDSVKCEVVDGGELKSRRHLNVRGKSATLPSITEKDWEDIKFGVENKVDFYAVSFVKDAQVVHELKKYLQNSGADIHVIVKIESADSIPNLHSIITASDGAMVARGDLGAELPIEEVPILQEEIINLCRSMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADAVMLSGETAHGKFPLKAAGVMHTVALRTEATITSGEMPPNLGQAFKNHMSEMFAYHATMMSNTLGTSTVVFTRTGFMAILLSHYRPSGTIYAFTNEKKIQQRLALYQGVCPIYMEFTDDAEETFANALATLLKQGMVKKGEEIAIVQSGTQPIWRSQSTHNIQVRKV

Gene Information

Entrez Gene ID
835369
Gene Name
plastidial pyruvate kinase 2
Gene Symbol
PKP-BETA1
Species
Arabidopsis thaliana

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0009507 IDA:TAIR C chloroplast
GO:0009570 IDA:TAIR C chloroplast stroma
GO:0005739 IDA:TAIR C mitochondrion
GO:0005524 IEA:UniProtKB-KW F ATP binding
GO:0000287 IDA:UniProtKB F magnesium ion binding
GO:0030955 IDA:UniProtKB F potassium ion binding
GO:0004743 IDA:TAIR F pyruvate kinase activity
GO:0006633 IMP:TAIR P fatty acid biosynthetic process
GO:0006096 IEA:UniProtKB-UniPathway P glycolytic process
GO:0006629 IGI:TAIR P lipid metabolic process
GO:0046686 IEP:TAIR P response to cadmium ion
GO:0048316 IMP:TAIR P seed development
GO:0010431 IMP:UniProtKB P seed maturation

KEGG Pathway Links

KEGG Pathway ID Description
ath01230 Biosynthesis of amino acids
ath01200 Carbon metabolism
ath00010 Glycolysis / Gluconeogenesis
ath_M00050 Guanine ribonucleotide biosynthesis IMP => GDP,GTP

Domain Information

InterPro Annotations

Accession Description
IPR001697 Pyruvate kinase
IPR015795 Pyruvate kinase, C-terminal
IPR018209 Pyruvate kinase, active site
IPR015794 Pyruvate kinase, alpha/beta
IPR015793 Pyruvate kinase, barrel
IPR015806 Pyruvate kinase, beta-barrel insert domain
IPR011037 Pyruvate kinase-like, insert domain
IPR015813 Pyruvate/Phosphoenolpyruvate kinase-like domain

UniProt Annotations

Entry Information

Gene Name
plastidial pyruvate kinase 2
Protein Entry
PKP2_ARATH
UniProt ID
Q9FLW9
Species
Arabidopsis

Comments

Comment Type Description
Biophysicochemical Properties pH dependence: Optimum pH is 7.8-8.0. {ECO:0000269|PubMed:17557808, ECO:0000269|PubMed:17892448};
Catalytic Activity ATP + pyruvate = ADP + phosphoenolpyruvate.
Cofactor Name=K(+); Xref=ChEBI:CHEBI:29103; Evidence={ECO:0000269|PubMed:17557808};
Cofactor Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000269|PubMed:17557808};
Developmental Stage In seeds, accumulates in endosperm and embryo. In torpedo-shaped embryos, restricted to the hypocotyl and in the outer parts of the young cotyledons. In later embryo stages, present in all tissues except root tips. {ECO:0000269|PubMed:17892448}.
Disruption Phenotype Reduced plastidial pyruvate kinase activity and altered seed oil content leading to wrinkled seeds, retarded embryo elongation and reduced seed germination. {ECO:0000269|PubMed:17557808, ECO:0000269|PubMed:17892448}.
Function Required for plastidial pyruvate kinase activity. Involved in seed oil accumulation, embryo development and seed storage compounds mobilization upon germination. {ECO:0000269|PubMed:17557808, ECO:0000269|PubMed:17892448, ECO:0000269|PubMed:17965177}.
Induction Repressed by cadmium. {ECO:0000269|PubMed:16502469}.
Pathway Carbohydrate degradation; glycolysis; pyruvate from D- glyceraldehyde 3-phosphate: step 5/5.
Similarity Belongs to the pyruvate kinase family. {ECO:0000305}.
Subcellular Location Plastid, chloroplast stroma. Mitochondrion.
Subunit Oligomer of alpha and beta subunits. {ECO:0000269|PubMed:17557808}.
Tissue Specificity Mostly expressed in seeds, and, to a lower extent, in roots, leaves (veins and trichomes), inflorescences, siliques, pollen (grains and tubes) and flowers (sepals and petals). {ECO:0000269|PubMed:17557808, ECO:0000269|PubMed:17892448}.

Identical and Related Proteins

Unique RefSeq proteins for LMP010922 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
15237303 RefSeq NP_200104 579 plastidial pyruvate kinase 2

Identical Sequences to LMP010922 proteins

Reference Database Accession Length Protein Name
GI:15237303 EMBL CCA78558.1 579 unnamed protein product [Arabidopsis thaliana]
GI:15237303 EMBL CDH48181.1 579 unnamed protein product [Arabidopsis thaliana]
GI:15237303 EMBL CDI44517.1 579 unnamed protein product [Arabidopsis thaliana]
GI:15237303 EMBL CDM63700.1 579 unnamed protein product [Arabidopsis thaliana]
GI:15237303 EMBL CDM63714.1 579 unnamed protein product [Arabidopsis thaliana]
GI:15237303 GenBank AGM79981.1 579 Sequence 28 from patent US 8426676

Related Sequences to LMP010922 proteins

Reference Database Accession Length Protein Name
GI:15237303 EMBL CBF66087.1 579 unnamed protein product [Arabidopsis thaliana]
GI:15237303 EMBL CCA78925.1 579 unnamed protein product [Arabidopsis thaliana]
GI:15237303 EMBL CCA78563.1 579 unnamed protein product [Arabidopsis thaliana]
GI:15237303 EMBL CDI44522.1 579 unnamed protein product [Arabidopsis thaliana]
GI:15237303 GenBank AAK82461.1 579 AT5g52920/MXC20_15 [Arabidopsis thaliana]
GI:15237303 GenBank AGX58052.1 579 Sequence 16417 from patent US 8541208