Gene/Proteome Database (LMPD)

LMPD ID
LMP010923
Gene ID
840138
Species
Arabidopsis thaliana (Arabidopsis)
Gene Name
plastidial pyruvate kinase 3
Gene Symbol
PKp3
Synonyms
F5D14.22; F5D14_22; F5F19.10; F5F19_10; PKp3; plastidial pyruvate kinase 3
Alternate Names
plastidial pyruvate kinase 3
Chromosome
1
EC Number
2.7.1.40
Summary
encodes a chloroplast pyruvate kinase beta subunit. The enzyme is less active than the other chloroplast pyruvate kinase beta subunit encoded by AT5G52920. Involved in seed oil biosynthesis. Can partially complement the AT5G52920 mutant.
Orthologs
View orthologs and multiple alignments for PKp3

Proteins

plastidial pyruvate kinase 3
Refseq ID NP_564402
Protein GI 18398434
UniProt ID Q93Z53
mRNA ID NM_102979
Length 571
RefSeq Status REVIEWED
MAAYGQISSGMTVDPQVLSSSRNIGVSLSPLRRTLIGAGVRSTSISLRQCSLSVRSIKISEDSRKPKAYAENGAFDVGVLDSSSYRLADSRTSSNDSRRKTKIVCTIGPSSSSREMIWKLAEAGMNVARLNMSHGDHASHQITIDLVKEYNSLFVDKAIAIMLDTKGPEVRSGDVPQPIFLEEGQEFNFTIKRGVSLKDTVSVNYDDFVNDVEVGDILLVDGGMMSLAVKSKTSDLVKCVVIDGGELQSRRHLNVRGKSATLPSITDKDWEDIKFGVDNQVDFYAVSFVKDAKVVHELKNYLKTCSADISVIVKIESADSIKNLPSIISACDGAMVARGDLGAELPIEEVPLLQEEIIRRCRSIHKPVIVATNMLESMINHPTPTRAEVSDIAIAVREGADAIMLSGETAHGKFPLKAVNVMHTVALRTEASLPVRTSASRTTAYKGHMGQMFAFHASIMANTLSSPLIVFTRTGSMAVLLSHYRPSATIFAFTNQRRIMQRLALYQGVMPIYMEFSDDAEDTYARSLKLLQDENMLKEGQHVTLVQSGSQPIWREESTHLIQVRKIKIGG

Gene Information

Entrez Gene ID
840138
Gene Name
plastidial pyruvate kinase 3
Gene Symbol
PKp3
Species
Arabidopsis thaliana

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0009507 IDA:TAIR C chloroplast
GO:0009570 IDA:TAIR C chloroplast stroma
GO:0005524 IEA:UniProtKB-KW F ATP binding
GO:0000287 IDA:UniProtKB F magnesium ion binding
GO:0030955 IDA:UniProtKB F potassium ion binding
GO:0004743 IDA:TAIR F pyruvate kinase activity
GO:0006633 IGI:TAIR P fatty acid biosynthetic process
GO:0006096 IEA:UniProtKB-UniPathway P glycolytic process
GO:0010431 ISS:UniProtKB P seed maturation

KEGG Pathway Links

KEGG Pathway ID Description
ath01230 Biosynthesis of amino acids
ath01200 Carbon metabolism
ath00010 Glycolysis / Gluconeogenesis
ath_M00050 Guanine ribonucleotide biosynthesis IMP => GDP,GTP

Domain Information

InterPro Annotations

Accession Description
IPR001697 Pyruvate kinase
IPR018209 Pyruvate kinase, active site
IPR015794 Pyruvate kinase, alpha/beta
IPR015793 Pyruvate kinase, barrel
IPR015806 Pyruvate kinase, beta-barrel insert domain
IPR015795 Pyruvate kinase, C-terminal
IPR011037 Pyruvate kinase-like, insert domain
IPR015813 Pyruvate/Phosphoenolpyruvate kinase-like domain

UniProt Annotations

Entry Information

Gene Name
plastidial pyruvate kinase 3
Protein Entry
PKP3_ARATH
UniProt ID
Q93Z53
Species
Arabidopsis

Comments

Comment Type Description
Biophysicochemical Properties pH dependence: Optimum pH is 7.8-8.0. {ECO:0000269|PubMed:17557808};
Catalytic Activity ATP + pyruvate = ADP + phosphoenolpyruvate.
Cofactor Name=K(+); Xref=ChEBI:CHEBI:29103; Evidence={ECO:0000269|PubMed:17557808};
Cofactor Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000269|PubMed:17557808};
Function Required for plastidial pyruvate kinase activity. {ECO:0000250}.
Pathway Carbohydrate degradation; glycolysis; pyruvate from D- glyceraldehyde 3-phosphate: step 5/5.
Sequence Caution Sequence=AAF81342.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
Similarity Belongs to the pyruvate kinase family. {ECO:0000305}.
Subcellular Location Plastid, chloroplast stroma {ECO:0000269|PubMed:17557808, ECO:0000269|PubMed:18431481}.
Subunit Oligomer of alpha and beta subunits. {ECO:0000269|PubMed:17557808}.
Tissue Specificity Expressed at low levels in roots, leaves, inflorescences, siliques, pollen, seeds and flowers. {ECO:0000269|PubMed:17557808, ECO:0000269|PubMed:17892448}.

Identical and Related Proteins

Unique RefSeq proteins for LMP010923 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
18398434 RefSeq NP_564402 571 plastidial pyruvate kinase 3

Identical Sequences to LMP010923 proteins

Reference Database Accession Length Protein Name
GI:18398434 EMBL CCA78921.1 571 unnamed protein product [Arabidopsis thaliana]
GI:18398434 EMBL CCA78559.1 571 unnamed protein product [Arabidopsis thaliana]
GI:18398434 EMBL CDH48182.1 571 unnamed protein product [Arabidopsis thaliana]
GI:18398434 EMBL CDI44518.1 571 unnamed protein product [Arabidopsis thaliana]
GI:18398434 GenBank AEE31487.1 571 plastidial pyruvate kinase 3 [Arabidopsis thaliana]
GI:18398434 GenBank AGX58051.1 571 Sequence 16415 from patent US 8541208

Related Sequences to LMP010923 proteins

Reference Database Accession Length Protein Name
GI:18398434 EMBL CAV28483.1 571 unnamed protein product [Arabidopsis thaliana]
GI:18398434 EMBL CBN64987.1 567 unnamed protein product [Arabidopsis thaliana]
GI:18398434 EMBL CDM63698.1 571 unnamed protein product [Arabidopsis thaliana]
GI:18398434 EMBL CDM63712.1 571 unnamed protein product [Arabidopsis thaliana]
GI:18398434 GenBank AAF81342.1 567 Strong similarity to a pyruvate kinase isozyme G, chloroplast precursor from Nicotiana tabacum gb|Z28374. It contains a pyruvate kinase domain PF|00224. EST gb|AI996399 comes from this gene [Arabidopsis thaliana]
GI:18398434 GenBank AGM79979.1 571 Sequence 24 from patent US 8426676