Gene/Proteome Database (LMPD)

LMPD ID
LMP011008
Gene ID
824657
Species
Arabidopsis thaliana (Arabidopsis)
Gene Name
putative pectate lyase
Gene Symbol
PMR6
Synonyms
powdery mildew resistant 6
Alternate Names
putative pectate lyase
Chromosome
3
EC Number
4.2.2.2
Summary
Powdery mildew resistant mutant encodes a pectate lyase-like protein
Orthologs
View orthologs and multiple alignments for PMR6

Proteins

putative pectate lyase
Refseq ID NP_191052
Protein GI 22331796
UniProt ID Q93Z04
mRNA ID NM_115349
Length 501
RefSeq Status REVIEWED
MLLQNFSNTIFLLCLFFTLLSATKPLNLTLPHQHPSPDSVALHVIRSVNESLARRQLSSPSSSSSSSSSSSSSSCRTGNPIDDCWRCSDADWSTNRQRLADCSIGFGHGTLGGKNGKIYVVTDSSDNNPTNPTPGTLRYGVIQEEPLWIVFSSNMLIRLKQELIINSYKTLDGRGSAVHITGNGCLTLQYVQHIIIHNLHIYDCKPSAGFEKRGRSDGDGISIFGSQKIWVDHCSMSHCTDGLIDAVMGSTAITISNNYFTHHDEVMLLGHDDNYAPDTGMQVTIAFNHFGQGLVQRMPRCRRGYIHVVNNDFTEWKMYAIGGSGNPTINSQGNRYSAPSDPSAKEVTKRVDSKDDGEWSNWNWRTEGDLMENGAFFVASGEGMSSMYSKASSVDPKAASLVDQLTRNAGVFGGPRDDQGQSGNSYSPYGGDGGGGGSSGGSSGGGMDVMGGTTRGSSSSSGDDSNVFQMIFGSDAPSRPRLTLLFSLLMISVLSLSTLLL

Gene Information

Entrez Gene ID
824657
Gene Name
putative pectate lyase
Gene Symbol
PMR6
Species
Arabidopsis thaliana

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0031225 TAS:TAIR C anchored component of membrane
GO:0046658 ISS:TAIR C anchored component of plasma membrane
GO:0046872 IEA:UniProtKB-KW F metal ion binding
GO:0030570 ISS:TAIR F pectate lyase activity
GO:0042547 IMP:TAIR P cell wall modification involved in multidimensional cell growth
GO:0009814 IMP:TAIR P defense response, incompatible interaction
GO:0045490 IEA:UniProtKB-UniPathway P pectin catabolic process

KEGG Pathway Links

KEGG Pathway ID Description
ath00040 Pentose and glucuronate interconversions
ko00040 Pentose and glucuronate interconversions

Domain Information

InterPro Annotations

Accession Description
IPR018082 AmbAllergen
IPR002022 Pectate lyase/Amb allergen
IPR012334 Pectin lyase fold
IPR011050 Pectin lyase fold/virulence factor

UniProt Annotations

Entry Information

Gene Name
putative pectate lyase
Protein Entry
PLY13_ARATH
UniProt ID
Q93Z04
Species
Arabidopsis

Comments

Comment Type Description
Catalytic Activity Eliminative cleavage of (1->4)-alpha-D- galacturonan to give oligosaccharides with 4-deoxy-alpha-D-galact- 4-enuronosyl groups at their non-reducing ends.
Cofactor Name=Ca(2+); Xref=ChEBI:CHEBI:29108; Evidence={ECO:0000250}; Note=Binds 1 Ca(2+) ion. Required for its activity. {ECO:0000250};
Domain The C-terminal domain not found in other pectate lyase- like protein is required for PMR6 function since the pmr6-2 mutation confers resistance by introducing a frameshift in the mature mRNA which eliminates the C-terminal domain.
Function Susceptibility factor required for infection by most powdery mildews, but not by unrelated pathogens. Exact function not known, but clearly affects cell wall composition. {ECO:0000269|PubMed:12215508}.
Miscellaneous Pmr6 mutations are pleiotropic, indicating that PMR6 plays a unique role in normal plant growth and development. The increased resistance in mutants is not mediated by the constitutive activation of the SA-dependent or the JA/ethylene- dependent defense pathway.
Pathway Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy- D-gluconate from pectin: step 2/5.
Sequence Caution Sequence=CAB41092.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
Similarity Belongs to the polysaccharide lyase 1 family. {ECO:0000305}.
Subcellular Location Cell membrane {ECO:0000305}; Lipid-anchor, GPI-anchor {ECO:0000305}.
Tissue Specificity Expressed equally in mature leaves, buds, flowers, rosettes and roots. {ECO:0000269|PubMed:12215508}.

Identical and Related Proteins

Unique RefSeq proteins for LMP011008 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
22331796 RefSeq NP_191052 501 putative pectate lyase

Identical Sequences to LMP011008 proteins

Reference Database Accession Length Protein Name
GI:22331796 EMBL CBC93286.1 501 unnamed protein product [Arabidopsis thaliana]
GI:22331796 EMBL CBC97868.1 501 unnamed protein product [Arabidopsis thaliana]
GI:22331796 EMBL CBC77838.1 501 unnamed protein product [Arabidopsis thaliana]
GI:22331796 GenBank ACW94509.1 501 Sequence 13745 from patent US 7569389
GI:22331796 GenBank ADT60924.1 501 Sequence 2738 from patent US 7847156
GI:22331796 GenBank AEE79313.1 501 putative pectate lyase [Arabidopsis thaliana]

Related Sequences to LMP011008 proteins

Reference Database Accession Length Protein Name
GI:22331796 EMBL CAB41092.1 542 pectate lyase-like protein [Arabidopsis thaliana]
GI:22331796 GenBank ACW92844.1 545 Sequence 11490 from patent US 7569389
GI:22331796 GenBank EFH52536.1 490 hypothetical protein ARALYDRAFT_485907 [Arabidopsis lyrata subsp. lyrata]
GI:22331796 RefSeq XP_002876277.1 490 hypothetical protein ARALYDRAFT_485907 [Arabidopsis lyrata subsp. lyrata]
GI:22331796 RefSeq XP_010504322.1 501 PREDICTED: probable pectate lyase 13 [Camelina sativa]
GI:22331796 RefSeq XP_010427226.1 504 PREDICTED: probable pectate lyase 13 [Camelina sativa]