Gene/Proteome Database (LMPD)

LMPD ID
LMP011131
Gene ID
819306
Species
Arabidopsis thaliana (Arabidopsis)
Gene Name
protein phosphatase 2C
Gene Symbol
POL
Synonyms
F14M4.25; POL; poltergeist
Alternate Names
protein phosphatase 2C
Chromosome
2
EC Number
3.1.3.16
Summary
Pol mutations are recessive, partial suppressors of meristem defects in strong clv1 and clv3 mutants, and nearly complete suppressors of weak clv1 mutants. Single mutants appear normal. Acts downstream of the CLV signaling pathway in meristem development and is required together with PLL1 for stem-cell maintenance through the regulation of WUS.
Orthologs
View orthologs and multiple alignments for POL

Proteins

protein phosphatase 2C
Refseq ID NP_850463
Protein GI 30690550
UniProt ID Q8RWN7
mRNA ID NM_180132
Length 856
RefSeq Status REVIEWED
MGNGTSRVVGCFVPSNDKNGVDLEFLEPLDEGLGHSFCYVRPSIFESPDITPSNSERFTIDSSTIDSETLTGSFRNDIVDDPSFLNRHNSKGLAETTFKAISGASVSANVSTARTGNQMALCSSDVLEPAASFESTSSFASIPLQPLPRGGSGPLNGFMSGPLERGFASGPLDRNNGFMSGPIEKGVMSGPLDVSDRSNFSAPLSFRRKKPRFQRFMRSVSGPMKSTLARTFSRRSGGLSWMHRFFLHPETRVSWAVGKDGKLHGEDPESCLESNRNLQWAHGKAGEDRVHVVLSEEQGWLFIGIYDGFSGPDAPDFVMSHLYKAIDKELEGLLWDYEEPSEDNQLQPDQEPPTEENMCDPESISEQHSKSVVAESEEVMIDDISSLGNTDTQIADGPPGDSAGPGKKSMRLYELLQLEQWEGEEIGLKRYGGNVALNNMTNQVENPSTSGGGAGNDPCTTDRSALDGIPNSGQRHGTKKSQISSKIRRMYQKQKSLRKKLFPWSYDWHREEGICVEEKIVESSGPIRRRWSGTVDHDAVLRAMARALESTEEAYMDMVEKSLDINPELALMGSCVLVMLMKDQDVYVMNVGDSRAILAQERLHDRHSNPGFGNDEGIGHKSRSRESLVRIELDRISEESPIHNQATPISVSNKNRDVTSYRLKMRAVQLSSDHSTSVEEEIWRIRSEHPEDDQSILKDRVKGQLKVTRAFGAGFLKKPNFNEALLEMFQVEYIGTDPYITCEPCTVHHRLTSSDRFMVLSSDGLYEYFSNEEVVAHVTWFIENVPEGDPAQYLIAELLSRAATKNGMEFHDLLDIPQGDRRKYHDDVSVMVVSLEGRIWRSSGQYYPERKQKFNR
protein phosphatase 2C
Refseq ID NP_850464
Protein GI 30690552
UniProt ID Q8RWN7
mRNA ID NM_180133
Length 856
RefSeq Status REVIEWED
Protein sequence is identical to GI:30690550 (mRNA isoform)

Gene Information

Entrez Gene ID
819306
Gene Name
protein phosphatase 2C
Gene Symbol
POL
Species
Arabidopsis thaliana

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005634 IEA:UniProtKB-KW C nucleus
GO:0005886 IDA:TAIR C plasma membrane
GO:0046872 IEA:UniProtKB-KW F metal ion binding
GO:0005543 IDA:TAIR F phospholipid binding
GO:0004722 IDA:TAIR F protein serine/threonine phosphatase activity
GO:0016311 IDA:GOC P dephosphorylation
GO:0010074 IGI:TAIR P maintenance of meristem identity
GO:0009934 IGI:TAIR P regulation of meristem structural organization
GO:0006355 IGI:TAIR P regulation of transcription, DNA-templated

Domain Information

InterPro Annotations

Accession Description
IPR015655 Protein phosphatase 2C
IPR001932 Protein phosphatase 2C (PP2C)-like domain

UniProt Annotations

Entry Information

Gene Name
protein phosphatase 2C
Protein Entry
P2C32_ARATH
UniProt ID
Q8RWN7
Species
Arabidopsis

Comments

Comment Type Description
Catalytic Activity [a protein]-serine/threonine phosphate + H(2)O = [a protein]-serine/threonine + phosphate.
Cofactor Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250}; Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000250}; Note=Binds 2 magnesium or manganese ions per subunit. {ECO:0000250};
Developmental Stage Expressed throughout development of the floral meristem, in all four floral whorls and as floral organs matured, limited to the ovule. {ECO:0000269|PubMed:12573213}.
Disruption Phenotype Loss-of-function mutant pol-6 (T-DNA insertion) shows suppression of clavata mutant phenotypes. The catalytic activity of POL may be functionally replaced by the activity of PLL1. Pol and pll1 double mutant is seedling lethal. {ECO:0000269|PubMed:16112663}.
Domain The N-terminal domain (1-233) has a regulatory function and inhibits phosphatase activity.
Domain The conserved PP2C phosphatase domain (271-833) is interrupted by an insertion of approximately 200 amino acids.
Enzyme Regulation Insensitive to okadaic acid. {ECO:0000269|PubMed:12573213}.
Function Involved in the regulation of pedicel length and of CLAVATA pathways controlling stem cell identity at shoot and flower meristems. {ECO:0000269|PubMed:10725242, ECO:0000269|PubMed:12573213, ECO:0000269|PubMed:16112663, ECO:0000269|PubMed:17079273}.
Sequence Caution Sequence=AAC34239.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
Similarity Belongs to the PP2C family. {ECO:0000305}.
Similarity Contains 1 PP2C-like domain. {ECO:0000305}.
Subcellular Location Nucleus {ECO:0000305}.
Tissue Specificity Expressed in roots, leaves, stems, inflorescences, flowers and throughout the shoot meristem. {ECO:0000269|PubMed:12573213, ECO:0000269|PubMed:16112663}.

Identical and Related Proteins

Unique RefSeq proteins for LMP011131 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
30690550 RefSeq NP_850463 856 protein phosphatase 2C

Identical Sequences to LMP011131 proteins

Reference Database Accession Length Protein Name
GI:30690550 GenBank AEC10772.1 856 protein phosphatase 2C [Arabidopsis thaliana]
GI:30690550 GenBank AEC10773.1 856 protein phosphatase 2C [Arabidopsis thaliana]
GI:30690550 RefSeq NP_850464.1 856 protein phosphatase 2C [Arabidopsis thaliana]
GI:30690550 SwissProt Q8RWN7.2 856 RecName: Full=Protein phosphatase 2C 32; Short=AtPP2C32; AltName: Full=Protein POLTERGEIST; AltName: Full=Protein phosphatase 2C POL; Short=PP2C POL [Arabidopsis thaliana]

Related Sequences to LMP011131 proteins

Reference Database Accession Length Protein Name
GI:30690550 DBBJ BAH56795.1 814 AT2G46920 [Arabidopsis thaliana]
GI:30690550 GenBank AAM12971.1 856 unknown protein [Arabidopsis thaliana]
GI:30690550 GenBank EOA26563.1 858 hypothetical protein CARUB_v10022621mg [Capsella rubella]
GI:30690550 gnl TIGR 814 unknown protein [Arabidopsis thaliana]
GI:30690550 RefSeq XP_006293665.1 858 hypothetical protein CARUB_v10022621mg [Capsella rubella]
GI:30690550 RefSeq XP_010506710.1 853 PREDICTED: protein phosphatase 2C 32 isoform X2 [Camelina sativa]