Gene/Proteome Database (LMPD)

LMPD ID
LMP011227
Gene ID
839031
Species
Arabidopsis thaliana (Arabidopsis)
Gene Name
pyruvate dehydrogenase E1 component subunit alpha-2
Gene Symbol
IAR4
Synonyms
F3I6.11; F3I6_11; IAA-CONJUGATE-RESISTANT 4; IAR4
Alternate Names
pyruvate dehydrogenase E1 component subunit alpha-2
Chromosome
1
EC Number
1.2.4.1
Summary
Arabidopsis thaliana pyruvate dehydrogenase E1a-like subunit. 81% identical to a previously characterized Arabidopsis mitochondrial PDH E1a-subunit, At1g59900
Orthologs
View orthologs and multiple alignments for IAR4

Proteins

pyruvate dehydrogenase E1 component subunit alpha-2
Refseq ID NP_173828
Protein GI 15221692
UniProt ID Q8H1Y0
mRNA ID NM_102264
Length 393
RefSeq Status REVIEWED
MALSRLSSRSNTFLKPAITALPSSIRRHVSTDSSPITIETAVPFTSHLCESPSRSVETSSEEILAFFRDMARMRRMEIAADSLYKAKLIRGFCHLYDGQEALAVGMEAAITKKDAIITSYRDHCTFIGRGGKLVDAFSELMGRKTGCSHGKGGSMHFYKKDASFYGGHGIVGAQIPLGCGLAFAQKYNKDEAVTFALYGDGAANQGQLFEALNISALWDLPAILVCENNHYGMGTATWRSAKSPAYFKRGDYVPGLKVDGMDALAVKQACKFAKEHALKNGPIILEMDTYRYHGHSMSDPGSTYRTRDEISGVRQVRDPIERVRKLLLTHDIATEKELKDMEKEIRKEVDDAVAQAKESPIPDASELFTNMYVKDCGVESFGADRKELKVTLP

Gene Information

Entrez Gene ID
839031
Gene Name
pyruvate dehydrogenase E1 component subunit alpha-2
Gene Symbol
IAR4
Species
Arabidopsis thaliana

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005829 IDA:TAIR C cytosol
GO:0005739 IDA:TAIR C mitochondrion
GO:0005634 IDA:TAIR C nucleus
GO:0050897 IDA:TAIR F cobalt ion binding
GO:0004739 IEA:UniProtKB-EC F pyruvate dehydrogenase (acetyl-transferring) activity
GO:0008270 IDA:TAIR F zinc ion binding
GO:0006096 IEA:UniProtKB-KW P glycolytic process
GO:0009651 IEP:TAIR P response to salt stress

KEGG Pathway Links

KEGG Pathway ID Description
ath01200 Carbon metabolism
ath00020 Citrate cycle (TCA cycle)
ath00010 Glycolysis / Gluconeogenesis
ath_M00307 Pyruvate oxidation, pyruvate => acetyl-CoA

Domain Information

InterPro Annotations

Accession Description
IPR001017 Dehydrogenase, E1 component
IPR017597 Pyruvate dehydrogenase (acetyl-transferring) E1 component, alpha subunit, subgroup y
IPR029061 Thiamin diphosphate-binding fold

UniProt Annotations

Entry Information

Gene Name
pyruvate dehydrogenase E1 component subunit alpha-2
Protein Entry
ODPA2_ARATH
UniProt ID
Q8H1Y0
Species
Arabidopsis

Comments

Comment Type Description
Catalytic Activity Pyruvate + [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine = [dihydrolipoyllysine-residue acetyltransferase] S-acetyldihydrolipoyllysine + CO(2).
Cofactor Name=thiamine diphosphate; Xref=ChEBI:CHEBI:58937;
Disruption Phenotype Reduced sensitivity to several IAA-amino acid conjugates. {ECO:0000269|PubMed:15173569}.
Enzyme Regulation E1 activity is regulated by phosphorylation (inactivation) and dephosphorylation (activation) of the alpha subunit. {ECO:0000250}.
Function The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3).
Subcellular Location Mitochondrion matrix {ECO:0000269|PubMed:14671022}.
Subunit Tetramer of 2 alpha and 2 beta subunits. {ECO:0000250}.

Identical and Related Proteins

Unique RefSeq proteins for LMP011227 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
15221692 RefSeq NP_173828 393 pyruvate dehydrogenase E1 component subunit alpha-2

Identical Sequences to LMP011227 proteins

Reference Database Accession Length Protein Name
GI:15221692 GenBank AAM65647.1 393 pyruvate dehydrogenase E1 alpha subunit [Arabidopsis thaliana]
GI:15221692 GenBank ACH11306.1 393 Sequence 38 from patent US 7396979
GI:15221692 GenBank ACX15589.1 393 Sequence 42412 from patent US 7569389
GI:15221692 GenBank ACX19602.1 393 Sequence 47840 from patent US 7569389
GI:15221692 GenBank AEE30492.1 393 pyruvate dehydrogenase E1 component subunit alpha-2 [Arabidopsis thaliana]
GI:15221692 SwissProt Q8H1Y0.2 393 RecName: Full=Pyruvate dehydrogenase E1 component subunit alpha-2, mitochondrial; Short=PDHE1-A; AltName: Full=Protein IAA-CONJUGATE-RESISTANT 4; Flags: Precursor [Arabidopsis thaliana]

Related Sequences to LMP011227 proteins

Reference Database Accession Length Protein Name
GI:15221692 GenBank AAN15218.1 393 pyruvate dehydrogenase E1a-like subunit IAR4 [Arabidopsis thaliana]
GI:15221692 GenBank EFH69589.1 393 IAA-conjugate-resistant 4 [Arabidopsis lyrata subsp. lyrata]
GI:15221692 GenBank AEL85852.1 393 Sequence 1988 from patent US 7989676
GI:15221692 GenBank EOA40424.1 442 hypothetical protein CARUB_v10009149mg [Capsella rubella]
GI:15221692 RefSeq XP_002893330.1 393 IAA-conjugate-resistant 4 [Arabidopsis lyrata subsp. lyrata]
GI:15221692 RefSeq XP_006307526.1 442 hypothetical protein CARUB_v10009149mg [Capsella rubella]