Gene/Proteome Database (LMPD)

LMPD ID
LMP011285
Gene ID
821259
Species
Arabidopsis thaliana (Arabidopsis)
Gene Name
SNF1-related protein kinase catalytic subunit alpha KIN10
Gene Symbol
KIN10
Synonyms
AKIN10; KIN10; SNF1 kinase homolog 10; SNF1-RELATED PROTEIN KINASE; SNF1-RELATED PROTEIN KINASE 1.1; SNRK1.1; T4P13.22; T4P13_22
Alternate Names
SNF1-related protein kinase catalytic subunit alpha KIN10
Chromosome
3
EC Number
2.7.11.1
Summary
encodes a SNF1-related protein kinase that physically interacts with SCF subunit SKP1/ASK1 and 20S proteosome subunit PAD1. It can also interact with PRL1 DWD-containing protein. Based on in vitro degradation assays and cul4cs and prl1 mutants, there is evidence that AKIN10 is degraded in a proteasome-dependent manner, and that this depends on a CUL4-PRL1 E3 ligase
Orthologs
View orthologs and multiple alignments for KIN10

Proteins

SNF1-related protein kinase catalytic subunit alpha KIN10
Refseq ID NP_001118546
Protein GI 186509642
UniProt ID Q38997
mRNA ID NM_001125074
Length 512
RefSeq Status REVIEWED
MDGSGTGSRSGVESILPNYKLGRTLGIGSFGRVKIAEHALTGHKVAIKILNRRKIKNMEMEEKVRREIKILRLFMHPHIIRLYEVIETPTDIYLVMEYVNSGELFDYIVEKGRLQEDEARNFFQQIISGVEYCHRNMVVHRDLKPENLLLDSKCNVKIADFGLSNIMRDGHFLKTSCGSPNYAAPEVISGKLYAGPEVDVWSCGVILYALLCGTLPFDDENIPNLFKKIKGGIYTLPSHLSPGARDLIPRMLVVDPMKRVTIPEIRQHPWFQAHLPRYLAVPPPDTVQQAKKIDEEILQEVINMGFDRNHLIESLRNRTQNDGTVTYYLILDNRFRASSGYLGAEFQETMEGTPRMHPAESVASPVSHRLPGLMEYQGVGLRSQYPVERKWALGLQSRAHPREIMTEVLKALQDLNVCWKKIGHYNMKCRWVPNSSADGMLSNSMHDNNYFGDESSIIENEAAVKSPNVVKFEIQLYKTRDDKYLLDLQRVQGPQFLFLDLCAAFLAQLRVL
SNF1-related protein kinase catalytic subunit alpha KIN10
Refseq ID NP_566130
Protein GI 18395701
UniProt ID Q38997
mRNA ID NM_110974
Length 512
RefSeq Status REVIEWED
Protein sequence is identical to GI:186509642 (mRNA isoform)
SNF1-related protein kinase catalytic subunit alpha KIN10
Refseq ID NP_850488
Protein GI 30678280
UniProt ID Q38997
mRNA ID NM_180157
Length 535
RefSeq Status REVIEWED
MFKRVDEFNLVSSTIDHRIFKSRMDGSGTGSRSGVESILPNYKLGRTLGIGSFGRVKIAEHALTGHKVAIKILNRRKIKNMEMEEKVRREIKILRLFMHPHIIRLYEVIETPTDIYLVMEYVNSGELFDYIVEKGRLQEDEARNFFQQIISGVEYCHRNMVVHRDLKPENLLLDSKCNVKIADFGLSNIMRDGHFLKTSCGSPNYAAPEVISGKLYAGPEVDVWSCGVILYALLCGTLPFDDENIPNLFKKIKGGIYTLPSHLSPGARDLIPRMLVVDPMKRVTIPEIRQHPWFQAHLPRYLAVPPPDTVQQAKKIDEEILQEVINMGFDRNHLIESLRNRTQNDGTVTYYLILDNRFRASSGYLGAEFQETMEGTPRMHPAESVASPVSHRLPGLMEYQGVGLRSQYPVERKWALGLQSRAHPREIMTEVLKALQDLNVCWKKIGHYNMKCRWVPNSSADGMLSNSMHDNNYFGDESSIIENEAAVKSPNVVKFEIQLYKTRDDKYLLDLQRVQGPQFLFLDLCAAFLAQLRVL

Gene Information

Entrez Gene ID
821259
Gene Name
SNF1-related protein kinase catalytic subunit alpha KIN10
Gene Symbol
KIN10
Species
Arabidopsis thaliana

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0000152 IPI:TAIR C nuclear ubiquitin ligase complex
GO:0005524 IEA:UniProtKB-KW F ATP binding
GO:0004674 IDA:TAIR F protein serine/threonine kinase activity
GO:0009738 IMP:TAIR P abscisic acid-activated signaling pathway
GO:0005975 IEA:UniProtKB-KW P carbohydrate metabolic process
GO:0009594 IDA:TAIR P detection of nutrient
GO:0003006 IMP:TAIR P developmental process involved in reproduction
GO:0006633 IEA:UniProtKB-KW P fatty acid biosynthetic process
GO:0042128 IEA:UniProtKB-KW P nitrate assimilation
GO:0010260 IMP:TAIR P organ senescence
GO:0080022 IMP:TAIR P primary root development
GO:0010182 IMP:TAIR P sugar mediated signaling pathway
GO:0010050 IMP:TAIR P vegetative phase change

KEGG Pathway Links

KEGG Pathway ID Description
ath04140 Regulation of autophagy

REACTOME Pathway Links

REACTOME Pathway ID Description
6253966 AMPK inhibits chREBP transcriptional activation activity
6254145 Regulation of AMPK activity via LKB1
6254302 Translocation of GLUT4 to the plasma membrane

Domain Information

InterPro Annotations

Accession Description
IPR028375 KA1 domain/Ssp2 C-terminal domain
IPR001772 Kinase associated domain 1 (KA1)
IPR000719 Protein kinase domain
IPR017441 Protein kinase, ATP binding site
IPR011009 Protein kinase-like domain
IPR008271 Serine/threonine-protein kinase, active site
IPR002290 Serine/threonine/dual specificity protein kinase, catalytic domain
IPR009060 UBA-like
IPR000449 Ubiquitin-associated domain/translation elongation factor EF-Ts, N-terminal
IPR015940 Ubiquitin-associated/translation elongation factor EF1B, N-terminal, eukaryote

UniProt Annotations

Entry Information

Gene Name
SNF1-related protein kinase catalytic subunit alpha KIN10
Protein Entry
KIN10_ARATH
UniProt ID
Q38997
Species
Arabidopsis

Comments

Comment Type Description
Alternative Products Event=Alternative splicing; Named isoforms=2; Name=1; IsoId=Q38997-1; Sequence=Displayed; Note=No experimental confirmation available.; Name=2; IsoId=Q38997-2; Sequence=VSP_009001;
Catalytic Activity ATP + a protein = ADP + a phosphoprotein.
Enzyme Regulation Activated by phosphorylation at Thr-198. {ECO:0000269|PubMed:19339507}.
Function Catalytic subunit of the probable trimeric SNF1-related protein kinase (SnRK) complex, which may play a role in a signal transduction cascade regulating gene expression and carbohydrate metabolism in higher plants. The SnRK complex may also be involved in the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase and in assimilation of nitrogen by phosphorylating nitrate reductase. In vitro, KIN10 exhibits kinase activity on sucrose phosphate synthase and the kinase activity is inhibited by PRL1. May be a subunit of a SCF ubiquitin ligase complex and thus be involved in proteasomal ubiquitination. Phosphorylates GRIK1/SNAK2 and GRIK2/SNAK1 in vitro. {ECO:0000269|PubMed:10220464, ECO:0000269|PubMed:11387208, ECO:0000269|PubMed:20164192}.
Induction Induced by sucrose. {ECO:0000269|PubMed:10220464}.
Interaction Q93V58:GRIK1; NbExp=2; IntAct=EBI-2107143, EBI-6399184; Q5HZ38:GRIK2; NbExp=2; IntAct=EBI-2107143, EBI-6399237; Q9SCY5:KINB2; NbExp=3; IntAct=EBI-2107143, EBI-2042436; Q42384:PRL1; NbExp=3; IntAct=EBI-2107143, EBI-1382964; Q944A6:SNF4; NbExp=2; IntAct=EBI-2107143, EBI-2360649;
Ptm Autophosphorylated. Phosphorylated at Thr-198 by GRIK1/SNAK2 and GRIK2/SNAK1. {ECO:0000269|PubMed:10220464, ECO:0000269|PubMed:19339507}.
Similarity Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily. {ECO:0000305}.
Similarity Contains 1 KA1 (kinase-associated) domain. {ECO:0000255|PROSITE-ProRule:PRU00565}.
Similarity Contains 1 UBA domain. {ECO:0000255|PROSITE- ProRule:PRU00212}.
Similarity Contains 1 protein kinase domain. {ECO:0000255|PROSITE-ProRule:PRU00159}.
Subunit Subunit of a probable heterotrimeric complex consisting of an alpha catalytic (KIN10 or KIN11) subunit, and a beta (KINB) and a gamma (KING or SNF4) non-catalytic regulatory subunits. Interacts with KINB2, KINB3, SNF4 and probably with KINB1 and KING1. Interacts with SKP1/ASK1, PAD1, the N-terminus of PRL1 and the WD40 domain of 5PTase13. Potential subunit of a SCF ubiquitin ligase complex consisting of a SNF1-related protein kinase, SKP1 and CUL1. The association of the SCF complex with the proteasome may be mediated by PAD1 and seems to be inhibited by the interaction with PRL1. Interacts with DSP4. {ECO:0000269|PubMed:10220464, ECO:0000269|PubMed:10929106, ECO:0000269|PubMed:11387208, ECO:0000269|PubMed:11522840, ECO:0000269|PubMed:15803412, ECO:0000269|PubMed:18931139}.
Web Resource Name=PlantP kinase Classification PPC; URL="http://plantsp.genomics.purdue.edu/family/class.html";

Identical and Related Proteins

Unique RefSeq proteins for LMP011285 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
30678280 RefSeq NP_850488 535 SNF1-related protein kinase catalytic subunit alpha KIN10
186509642 RefSeq NP_001118546 512 SNF1-related protein kinase catalytic subunit alpha KIN10

Identical Sequences to LMP011285 proteins

Reference Database Accession Length Protein Name
GI:186509642 EMBL CAA64384.1 512 ser/thr protein kinase [Arabidopsis thaliana]
GI:186509642 GenBank AAF26165.1 512 putative SNF1-related protein kinase [Arabidopsis thaliana]
GI:30678280 GenBank AAM13169.1 535 putative SNF1-related protein kinase [Arabidopsis thaliana]
GI:30678280 GenBank AAQ56829.1 535 At3g01090 [Arabidopsis thaliana]
GI:186509642 GenBank ABH11527.1 512 SNR2 [Arabidopsis thaliana]
GI:186509642 GenBank AEE73607.1 512 SNF1-related protein kinase catalytic subunit alpha KIN10 [Arabidopsis thaliana]
GI:30678280 GenBank AEE73608.1 535 SNF1-related protein kinase catalytic subunit alpha KIN10 [Arabidopsis thaliana]
GI:186509642 GenBank AEE73609.1 512 SNF1-related protein kinase catalytic subunit alpha KIN10 [Arabidopsis thaliana]
GI:186509642 RefSeq NP_566130.1 512 SNF1-related protein kinase catalytic subunit alpha KIN10 [Arabidopsis thaliana]
GI:30678280 SwissProt Q38997.2 535 RecName: Full=SNF1-related protein kinase catalytic subunit alpha KIN10; Short=AKIN10; AltName: Full=AKIN alpha-2; Short=AKINalpha2 [Arabidopsis thaliana]

Related Sequences to LMP011285 proteins

Reference Database Accession Length Protein Name
GI:186509642 DBBJ BAH19438.1 512 AT3G01090 [Arabidopsis thaliana]
GI:30678280 EMBL CAA64384.1 512 ser/thr protein kinase [Arabidopsis thaliana]
GI:30678280 GenBank AAA32736.1 512 SNF1-related protein kinase [Arabidopsis thaliana]
GI:30678280 GenBank AAF26165.1 512 putative SNF1-related protein kinase [Arabidopsis thaliana]
GI:186509642 GenBank AAM13169.1 535 putative SNF1-related protein kinase [Arabidopsis thaliana]
GI:186509642 GenBank AAQ56829.1 535 At3g01090 [Arabidopsis thaliana]
GI:30678280 GenBank ABH11527.1 512 SNR2 [Arabidopsis thaliana]
GI:30678280 GenBank AEE73607.1 512 SNF1-related protein kinase catalytic subunit alpha KIN10 [Arabidopsis thaliana]
GI:186509642 GenBank AEE73608.1 535 SNF1-related protein kinase catalytic subunit alpha KIN10 [Arabidopsis thaliana]
GI:30678280 GenBank AEE73609.1 512 SNF1-related protein kinase catalytic subunit alpha KIN10 [Arabidopsis thaliana]
GI:186509642 RefSeq NP_850488.1 535 SNF1-related protein kinase catalytic subunit alpha KIN10 [Arabidopsis thaliana]
GI:186509642 SwissProt Q38997.2 535 RecName: Full=SNF1-related protein kinase catalytic subunit alpha KIN10; Short=AKIN10; AltName: Full=AKIN alpha-2; Short=AKINalpha2 [Arabidopsis thaliana]