Gene/Proteome Database (LMPD)
LMPD ID
LMP011289
Gene ID
Species
Arabidopsis thaliana (Arabidopsis)
Gene Name
solanesyl diphosphate synthase 1
Gene Symbol
Synonyms
solanesyl diphosphate synthase 1; SPS1; T30F21.15; T30F21_15
Alternate Names
solanesyl diphosphate synthase 1
Chromosome
1
EC Number
2.5.1.85
Summary
Encodes a protein with solanesyl diphosphate synthase activity.
Orthologs
Proteins
| solanesyl diphosphate synthase 1 | |
|---|---|
| Refseq ID | NP_001077840 |
| Protein GI | 145327729 |
| UniProt ID | Q8S948 |
| mRNA ID | NM_001084371 |
| Length | 367 |
| RefSeq Status | REVIEWED |
| MAVWWGVAKRCQRNQRRSLCSMVCIGQSQTVSFDLKQESKQPISLVTLFELVAVDLQTLNDNLLSIVGAENPVLISAAEQIFGAGGKRMRPGLVFLVSHATAELAGLKELTTEHRRLAEIIEMIHTASLIHDDVLDESDMRRGKETVHELFGTRVAVLAGDFMFAQASWYLANLENLEVIKLISQVIKDFASGEIKQASSLFDCDTKLDEYLLKSFYKTASLVAASTKGAAIFSRVEPDVTEQMYEFGKNLGLSFQIVDDILDFTQSTEQLGKPAGSDLAKGNLTAPVIFALEREPRLREIIESEFCEAGSLEEAIEAVTKGGGIKRAQELAREKADDAIKNLQCLPRSGFRSALEDMVLYNLERID | |
| solanesyl diphosphate synthase 1 | |
|---|---|
| Refseq ID | NP_177972 |
| Protein GI | 30699332 |
| UniProt ID | Q8S948 |
| mRNA ID | NM_106498 |
| Length | 406 |
| RefSeq Status | REVIEWED |
| MMTSCRNIDLGTMMMACGCGRRQFPSLAKTVCKFTSSNRSYGGLVGSCKAVPTKSKEISLLNGIGQSQTVSFDLKQESKQPISLVTLFELVAVDLQTLNDNLLSIVGAENPVLISAAEQIFGAGGKRMRPGLVFLVSHATAELAGLKELTTEHRRLAEIIEMIHTASLIHDDVLDESDMRRGKETVHELFGTRVAVLAGDFMFAQASWYLANLENLEVIKLISQVIKDFASGEIKQASSLFDCDTKLDEYLLKSFYKTASLVAASTKGAAIFSRVEPDVTEQMYEFGKNLGLSFQIVDDILDFTQSTEQLGKPAGSDLAKGNLTAPVIFALEREPRLREIIESEFCEAGSLEEAIEAVTKGGGIKRAQELAREKADDAIKNLQCLPRSGFRSALEDMVLYNLERID | |
Gene Information
Entrez Gene ID
Gene Name
solanesyl diphosphate synthase 1
Gene Symbol
Species
Arabidopsis thaliana
Gene Ontology (GO Annotations)
| GO ID | Source | Type | Description |
|---|---|---|---|
| GO:0005783 | IEA:UniProtKB-KW | C | endoplasmic reticulum |
| GO:0009536 | IDA:TAIR | C | plastid |
| GO:0052924 | IEA:UniProtKB-EC | F | all-trans-nonaprenyl-diphosphate synthase (geranylgeranyl-diphosphate specific) activity |
| GO:0046872 | IEA:UniProtKB-KW | F | metal ion binding |
| GO:0050347 | IDA:TAIR | F | trans-octaprenyltranstransferase activity |
| GO:0008299 | IEA:UniProtKB-KW | P | isoprenoid biosynthetic process |
| GO:0015979 | IEA:InterPro | P | photosynthesis |
| GO:0010236 | IMP:TAIR | P | plastoquinone biosynthetic process |
KEGG Pathway Links
| KEGG Pathway ID | Description |
|---|---|
| ath01110 | Biosynthesis of secondary metabolites |
| ath00900 | Terpenoid backbone biosynthesis |
| ko00900 | Terpenoid backbone biosynthesis |
BIOCYC Pathway Links
| BIOCYC Pathway ID | Description |
|---|---|
| PWY-5807 | heptaprenyl diphosphate biosynthesis |
Domain Information
UniProt Annotations
Entry Information
Gene Name
solanesyl diphosphate synthase 1
Protein Entry
SPS1_ARATH
UniProt ID
Species
Arabidopsis
Comments
| Comment Type | Description |
|---|---|
| Alternative Products | Event=Alternative splicing; Named isoforms=2; Name=1; IsoId=Q8S948-1; Sequence=Displayed; Name=2; IsoId=Q8S948-2; Sequence=VSP_042134; Note=Derived from EST data. No experimental confirmation available.; |
| Biophysicochemical Properties | Kinetic parameters: KM=5.73 uM for farnesyl diphosphate (in the presence of 500 uM of isopentenyl diphosphate) {ECO:0000269|PubMed:12437513}; KM=1.61 uM for geranylgeranyl diphosphate (in the presence of 100 uM of isopentenyl diphosphate) {ECO:0000269|PubMed:12437513}; KM=151 uM for isopentenyl diphosphate (in the presence of 20 uM of farnesyl diphosphate) {ECO:0000269|PubMed:12437513}; KM=20 uM for isopentenyl diphosphate (in the presence of 4 uM of geranylgeranyl diphosphate) {ECO:0000269|PubMed:12437513}; Note=kcat is 29.7 sec(-1) with farnesyl diphosphate as substrate (in the presence of 500 uM of isopentenyl diphosphate). kcat is 21.0 sec(-1) with geranylgeranyl diphosphate as substrate (in the presence of 100 uM of isopentenyl diphosphate). kcat is 14.7 sec(-1) with isopentenyl diphosphate as substrate (in the presence of 20 uM of farnesyl diphosphate). kcat is 15.0 sec(-1) with isopentenyl diphosphate as substrate (in the presence of 4 uM of geranylgeranyl diphosphate).; pH dependence: Optimum pH is 8.0. {ECO:0000269|PubMed:12437513}; |
| Catalytic Activity | Geranylgeranyl diphosphate + 5 isopentenyl diphosphate = 5 diphosphate + all-trans-nonaprenyl diphosphate. {ECO:0000269|PubMed:12437513}. |
| Cofactor | Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250}; Note=Binds 3 Mg(2+) ions per subunit. {ECO:0000250}; |
| Function | Involved in the solanesyl diphosphate biosynthesis in the endoplasmic reticulum. This isoprenoid side chain might be then transferred to the mitochondria for the synthesis of ubiquinone-9. Prefers geranylgeranyl diphosphate to farnesyl diphosphate as substrate. No activity with geranyl diphosphate or dimethylallyl diphosphate as substrate. {ECO:0000269|PubMed:12437513, ECO:0000269|PubMed:15653808}. |
| Sequence Caution | Sequence=AAD30584.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305}; |
| Similarity | Belongs to the FPP/GGPP synthase family. {ECO:0000305}. |
| Subcellular Location | Endoplasmic reticulum {ECO:0000269|PubMed:15653808, ECO:0000269|PubMed:15784989}. |
| Subunit | Homodimer. {ECO:0000269|PubMed:12437513}. |
| Tissue Specificity | Higher expression in leaves than in roots. {ECO:0000269|PubMed:15784989}. |
Identical and Related Proteins
Unique RefSeq proteins for LMP011289 (as displayed in Record Overview)
| Protein GI | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| 30699332 | RefSeq | NP_177972 | 406 | solanesyl diphosphate synthase 1 |
| 145327729 | RefSeq | NP_001077840 | 367 | solanesyl diphosphate synthase 1 |
Identical Sequences to LMP011289 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:30699332 | DBBJ | BAB86941.1 | 406 | solanesyl diphosphate synthase [Arabidopsis thaliana] |
| GI:30699332 | DBBJ | BAD88533.1 | 406 | solanesyl diphosphate synthase 1 [Arabidopsis thaliana] |
| GI:30699332 | GenBank | ABF58968.1 | 406 | At1g78510 [Arabidopsis thaliana] |
| GI:30699332 | GenBank | AEE36114.1 | 406 | solanesyl diphosphate synthase 1 [Arabidopsis thaliana] |
| GI:145327729 | GenBank | AEE36115.1 | 367 | solanesyl diphosphate synthase 1 [Arabidopsis thaliana] |
| GI:30699332 | SwissProt | Q8S948.1 | 406 | RecName: Full=Solanesyl diphosphate synthase 1; Short=AtSPS1; AltName: Full=All-trans-nonaprenyl-diphosphate synthase 1 (geranylgeranyl-diphosphate specific) [Arabidopsis thaliana] |
Related Sequences to LMP011289 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:145327729 | DBBJ | BAB86941.1 | 406 | solanesyl diphosphate synthase [Arabidopsis thaliana] |
| GI:145327729 | DBBJ | BAD88533.1 | 406 | solanesyl diphosphate synthase 1 [Arabidopsis thaliana] |
| GI:30699332 | DBBJ | BAF01575.1 | 406 | polyprenyl diphosphate synthase [Arabidopsis thaliana] |
| GI:145327729 | GenBank | AAD30584.1 | 390 | Very similar to prenyltransferases [Arabidopsis thaliana] |
| GI:145327729 | GenBank | ABF58968.1 | 406 | At1g78510 [Arabidopsis thaliana] |
| GI:30699332 | GenBank | EFH65453.1 | 406 | solanesyl diphosphate synthase [Arabidopsis lyrata subsp. lyrata] |
| GI:145327729 | RefSeq | NP_177972.2 | 406 | solanesyl diphosphate synthase 1 [Arabidopsis thaliana] |
| GI:30699332 | RefSeq | XP_002889194.1 | 406 | solanesyl diphosphate synthase [Arabidopsis lyrata subsp. lyrata] |
| GI:30699332 | RefSeq | XP_010416827.1 | 411 | PREDICTED: solanesyl diphosphate synthase 1 isoform X1 [Camelina sativa] |
| GI:30699332 | RefSeq | XP_010428978.1 | 411 | PREDICTED: solanesyl diphosphate synthase 1-like isoform X1 [Camelina sativa] |
| GI:30699332 | RefSeq | XP_010428979.1 | 411 | PREDICTED: solanesyl diphosphate synthase 1-like isoform X2 [Camelina sativa] |
| GI:145327729 | SwissProt | Q8S948.1 | 406 | RecName: Full=Solanesyl diphosphate synthase 1; Short=AtSPS1; AltName: Full=All-trans-nonaprenyl-diphosphate synthase 1 (geranylgeranyl-diphosphate specific) [Arabidopsis thaliana] |