Gene/Proteome Database (LMPD)

LMPD ID
LMP011298
Gene ID
843300
Species
Arabidopsis thaliana (Arabidopsis)
Gene Name
sphingoid base hydroxylase 1
Gene Symbol
SBH1
Synonyms
F24J1.22; SBH1; sphingoid base hydroxylase 1
Alternate Names
sphingoid base hydroxylase 1
Chromosome
1
EC Number
1.14.13.169
Summary
Encodes one of the two redundant sphingoid base hydroxylases (SBH). Involved in sphingolipid trihydroxy long-chain base (4-hydroxysphinganine) biosynthesis. Double mutants of SBHs were dwarfed and not able to progress from vegetative to reproductive growth.
Orthologs
View orthologs and multiple alignments for SBH1

Proteins

sphingoid base hydroxylase 1
Refseq ID NP_177122
Protein GI 22330531
UniProt ID Q8VYI1
mRNA ID NM_105632
Length 260
RefSeq Status REVIEWED
MMMGFAVSDELLGTVAPIVVYWLYSGIYVALSSLESYRLHSKVEEEEKNLVSKSSVVKGVLVQQVVQAVVAILLFTVTGSDAEADKAQQFSFLVLARQFVTAMIVLDTWQYFMHRYMHQNKFLYKHIHSQHHRLIVPYAYGALYNHPVEGLLLDTIGGALSFLVSGMSPRTSIFFFSFATIKTVDDHCGLWLPGNLFHMVFKNNSAYHDIHHQLYGTKYNFSQPFFVMWDRILGTYMPYSLEKREDGGFEARPTKEFKDD

Gene Information

Entrez Gene ID
843300
Gene Name
sphingoid base hydroxylase 1
Gene Symbol
SBH1
Species
Arabidopsis thaliana

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005794 IDA:TAIR C Golgi apparatus
GO:0005783 IDA:TAIR C endoplasmic reticulum
GO:0016021 IEA:UniProtKB-KW C integral component of membrane
GO:0005506 IEA:InterPro F iron ion binding
GO:0000170 IGI:TAIR F sphingosine hydroxylase activity
GO:0006633 IEA:InterPro P fatty acid biosynthetic process
GO:0009640 IMP:TAIR P photomorphogenesis
GO:0046520 IMP:TAIR P sphingoid biosynthetic process

KEGG Pathway Links

KEGG Pathway ID Description
ath01100 Metabolic pathways
ath00600 Sphingolipid metabolism

BIOCYC Pathway Links

BIOCYC Pathway ID Description
PWY-5129 sphingolipid biosynthesis (plants)
PWY-5129 sphingolipid biosynthesis (plants)

Domain Information

InterPro Annotations

Accession Description
IPR006694 Fatty_acid_hydroxylase

UniProt Annotations

Entry Information

Gene Name
sphingoid base hydroxylase 1
Protein Entry
SBH1_ARATH
UniProt ID
Q8VYI1
Species
Arabidopsis

Comments

Comment Type Description
Catalytic Activity Sphinganine + NADPH + O(2) = phytosphingosine + NADP(+) + H(2)O. {ECO:0000269|PubMed:11297741}.
Cofactor Name=Fe cation; Xref=ChEBI:CHEBI:24875; Evidence={ECO:0000250};
Disruption Phenotype No visible phenotype; due to the redundancy with SBH2. Sbh1 and sbh2 double mutants are severely dwarfed, do not progress from vegetative to reproductive growth and have enhanced expression of programmed cell death associated-genes. {ECO:0000269|PubMed:18612100}.
Domain The histidine box domains may contain the active site and/or be involved in metal ion binding.
Function Involved in sphingolipid trihydroxy long-chain base (4- hydroxysphinganine) biosynthesis. Can use C18- and C20-sphinganine as substrates to produce C18- and C20-phytosphinganines (D-ribo-2- amino-1,3,4-trihydroxyoctadecane and -eicosane). {ECO:0000269|PubMed:11297741, ECO:0000269|PubMed:18612100}.
Pathway Membrane lipid metabolism; sphingolipid biosynthesis.
Sequence Caution Sequence=AAG12703.1; Type=Erroneous initiation; Note=Translation N-terminally extended.; Evidence={ECO:0000305}; Sequence=AAG52550.1; Type=Erroneous initiation; Note=Translation N-terminally extended.; Evidence={ECO:0000305};
Similarity Belongs to the sterol desaturase family. {ECO:0000305}.
Subcellular Location Endoplasmic reticulum membrane {ECO:0000269|PubMed:18612100}; Multi-pass membrane protein {ECO:0000269|PubMed:18612100}.
Tissue Specificity Ubiquitous, with higher levels in flowers and roots. {ECO:0000269|PubMed:18612100}.

Identical and Related Proteins

Unique RefSeq proteins for LMP011298 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
22330531 RefSeq NP_177122 260 sphingoid base hydroxylase 1

Identical Sequences to LMP011298 proteins

Reference Database Accession Length Protein Name
GI:22330531 GenBank AAL50102.1 260 At1g69640/F24J1.22 [Arabidopsis thaliana]
GI:22330531 GenBank AAN28857.1 260 At1g69640/F24J1.22 [Arabidopsis thaliana]
GI:22330531 GenBank AEE34958.1 260 sphingoid base hydroxylase 1 [Arabidopsis thaliana]
GI:22330531 SwissProt Q8VYI1.1 260 RecName: Full=Sphinganine C(4)-monooxygenase 1; AltName: Full=Sphingoid C4-hydroxylase 1; AltName: Full=Sphingoid base hydroxylase 1 [Arabidopsis thaliana]

Related Sequences to LMP011298 proteins

Reference Database Accession Length Protein Name
GI:22330531 GenBank AAG12703.1 258 acid phosphatase, putative; 5376-6903 [Arabidopsis thaliana]
GI:22330531 GenBank AAG52550.1 258 putative sterol desaturase; 75442-76969 [Arabidopsis thaliana]
GI:22330531 GenBank EFH64986.1 258 hypothetical protein ARALYDRAFT_476099 [Arabidopsis lyrata subsp. lyrata]
GI:22330531 RefSeq XP_002888727.1 258 hypothetical protein ARALYDRAFT_476099 [Arabidopsis lyrata subsp. lyrata]
GI:22330531 RefSeq XP_010512012.1 279 PREDICTED: sphinganine C(4)-monooxygenase 1-like [Camelina sativa]
GI:22330531 RefSeq XP_010470962.1 259 PREDICTED: sphinganine C(4)-monooxygenase 1 [Camelina sativa]