Gene/Proteome Database (LMPD)

LMPD ID
LMP011304
Gene ID
10723114
Species
Arabidopsis thaliana (Arabidopsis)
Gene Name
Diacylglycerol kinase family protein
Gene Symbol
AT4G21534
Alternate Names
Diacylglycerol kinase family protein
Chromosome
4
EC Number
2.7.1.91

Proteins

Diacylglycerol kinase family protein
Refseq ID NP_001190787
Protein GI 334186771
UniProt ID F2Y4A3
mRNA ID NM_001203858
Length 481
RefSeq Status REVIEWED
MENDQFMCPTDIITGIVFIDGELAMLTLTADGELRWTEYGLRQYLSMKKDVLGFIVEGKQIRVKAVVEKEAGGICCGQFGGDFVRKDFVFEPLIDQNGWCYKLRQYLDSLGRPKRLLVFVNPFGGKKSAREIFVKEVKPLFEDADVQLEIQETKYQLHAKEFVKSMDVSKYDGIVCVSGDGILVEVVNGLLERADWRNALKLPIGMVPAGTGNGMIKSLLDTVGLRCCANSATISIIRGHKRSVDVATIAQGNTKFFSVLMLAWGLIADIDIESEKFRWMGSARIDFYALQRIICLRRYNGRILFLPAPGFEGYGQPASCSLYQEPHVSDKEVGYQGPETKFEDLEWREMKGPFVTIWLHNVPWGSENTLTAPAAKFSDGYLDLIVLKNCPKLVLLSLMRQTSSGTHVESPYIVYIKVKAFVLEPGALVDEPDKEGIIDSDGEVLARGKRTYKCDQKALMSYDKLQVTVDQGLATLFSPEY

Gene Information

Entrez Gene ID
10723114
Gene Name
Diacylglycerol kinase family protein
Gene Symbol
AT4G21534
Species
Arabidopsis thaliana

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0009705 IDA:UniProtKB C plant-type vacuole membrane
GO:0005524 IEA:UniProtKB-KW F ATP binding
GO:0003951 IEA:InterPro F NAD+ kinase activity
GO:0004143 IEA:InterPro F diacylglycerol kinase activity
GO:0008481 IDA:UniProtKB F sphinganine kinase activity
GO:0071215 IMP:UniProtKB P cellular response to abscisic acid stimulus
GO:0046834 IDA:GOC P lipid phosphorylation
GO:0007205 IEA:InterPro P protein kinase C-activating G-protein coupled receptor signaling pathway
GO:0006950 IEA:UniProtKB-KW P response to stress
GO:0006665 IDA:UniProtKB P sphingolipid metabolic process

KEGG Pathway Links

KEGG Pathway ID Description
ath00600 Sphingolipid metabolism
ath_M00100 Sphingosine degradation

REACTOME Pathway Links

REACTOME Pathway ID Description
6254167 Association of TriC/CCT with target proteins during biosynthesis
6254191 Sphingolipid de novo biosynthesis

Domain Information

InterPro Annotations

Accession Description
IPR016064 ATP-NAD kinase-like domain
IPR001206 Diacylglycerol kinase, catalytic domain

UniProt Annotations

Entry Information

Gene Name
Diacylglycerol kinase family protein
Protein Entry
SPHK2_ARATH
UniProt ID
F2Y4A3
Species
Arabidopsis

Comments

Comment Type Description
Catalytic Activity ATP + sphinganine = ADP + sphinganine 1- phosphate. {ECO:0000269|PubMed:21330371}.
Catalytic Activity ATP + sphingosine = ADP + sphingosine 1- phosphate. {ECO:0000269|PubMed:21330371}.
Cofactor Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
Disruption Phenotype No visible phenotype under normal growth conditions. {ECO:0000269|PubMed:22275366}.
Enzyme Regulation Activated by phosphatidic acid (PA). Binding with PA stimulates the activity by promoting the binding of substrate to the catalytic site.
Function Involved in the production of sphingolipid metabolites. Phosphorylates sphingosine and various l sphingoid long-chain base (LCB) products, such as phytosphingosine (PHS, 4- hydroxysphinganine), 4-hydroxy-8-sphingenine, 4,8-sphingadienine and D-erythro-dihydrosphingosine, but has a very few activity toward D,L-threo- dihydrosphingosine. Is required for abscisic acid (ABA) signaling that mediates stomatal closure, inhibition of seed germination and root elongation. May function upstream of PLDALPHA1 and phosphatidic acid (PA) in an amplification response to ABA that mediates stomatal closure. {ECO:0000269|PubMed:21330371, ECO:0000269|PubMed:22275366}.
Sequence Caution Sequence=CAA18718.1; Type=Erroneous gene model prediction; Note=The predicted gene At4g21540 has been split into 2 genes: At4g21534 and At4g21540.; Evidence={ECO:0000305}; Sequence=CAB81261.1; Type=Erroneous gene model prediction; Note=The predicted gene At4g21540 has been split into 2 genes: At4g21534 and At4g21540.; Evidence={ECO:0000305};
Similarity Contains 1 DAGKc domain. {ECO:0000255|PROSITE- ProRule:PRU00783}.
Subcellular Location Vacuole membrane {ECO:0000305|PubMed:21330371}; Peripheral membrane protein {ECO:0000305|PubMed:21330371}. Note=Associated with the tonoplast.
Tissue Specificity Hihgly expressed in flowers and siliques and at lower levels in roots, leaves and stems. {ECO:0000269|PubMed:21330371}.

Identical and Related Proteins

Unique RefSeq proteins for LMP011304 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
334186771 RefSeq NP_001190787 481 Diacylglycerol kinase family protein

Identical Sequences to LMP011304 proteins

Reference Database Accession Length Protein Name
GI:334186771 GenBank ADZ38930.1 481 sphingosine kinase 2 [Arabidopsis thaliana]
GI:334186771 GenBank AEE84464.1 481 Diacylglycerol kinase family protein [Arabidopsis thaliana]
GI:334186771 SwissProt F2Y4A3.1 481 RecName: Full=Sphingosine kinase 2 [Arabidopsis thaliana]

Related Sequences to LMP011304 proteins

Reference Database Accession Length Protein Name
GI:334186771 EMBL CAA18718.1 1240 putative protein [Arabidopsis thaliana]
GI:334186771 EMBL CAB81261.1 1240 putative protein [Arabidopsis thaliana]
GI:334186771 EMBL CAW63220.1 1240 unnamed protein product [Arabidopsis thaliana]
GI:334186771 EMBL CBC44977.1 1274 unnamed protein product [Arabidopsis thaliana]
GI:334186771 EMBL CBC44981.1 1240 unnamed protein product [Arabidopsis thaliana]
GI:334186771 EMBL CBD34348.1 1274 unnamed protein product [Arabidopsis thaliana]