Gene/Proteome Database (LMPD)
LMPD ID
LMP011306
Gene ID
Species
Arabidopsis thaliana (Arabidopsis)
Gene Name
probable squalene epoxidase 1
Gene Symbol
Synonyms
F9K23.3; F9K23_3; SQE1; SQUALENE EPOXIDASE 1; XF1
Alternate Names
probable squalene epoxidase 1
Chromosome
1
EC Number
1.14.13.132
Summary
Encodes a putative protein that has been speculated, based on sequence similarities, to have squalene monooxygenase activity.
Orthologs
Proteins
| probable squalene epoxidase 1 | |
|---|---|
| Refseq ID | NP_564734 |
| Protein GI | 18406296 |
| UniProt ID | Q9SM02 |
| mRNA ID | NM_104624 |
| Length | 531 |
| RefSeq Status | REVIEWED |
| MESQLWNWILPLLISSLLISFVAFYGFFVKPKRNGLRHDRKTVSTVTSDVGSVNITGDTVADVIVVGAGVAGSALAYTLGKDKRRVHVIERDLSEPDRIVGELLQPGGYLKLLELGIEDCVEEIDAQRVYGYALFKNGKRIRLAYPLEKFHEDVSGRSFHNGRFIQRMREKAASLPNVQLEQGTVLSLLEENGTIKGVRYKNKAGEEQTAFAALTIVCDGCFSNLRRSLCNPQVEVPSCFVGLVLENCNLPYANHGHVVLADPSPILMYPISSTEVRCLVDVPGQKVPSIANGEMKNYLKTVVAPQMPHEVYDSFIAAVDKGNIKSMPNRSMPASPYPTPGALLMGDAFNMRHPLTGGGMTVALADIVVLRNLLRPLRDLSDGASLCKYLESFYTLRKPVAATINTLANALYQVFCSSENEARNEMREACFDYLGLGGMCTSGPVSLLSGLNPRPLTLVCHFFAVAVYGVIRLLIPFPSPKRIWLGAKLISGASGIIFPIIKAEGVRQMFFPATVPAYYYKAPTVGETKCS | |
Gene Information
Entrez Gene ID
Gene Name
probable squalene epoxidase 1
Gene Symbol
Species
Arabidopsis thaliana
Gene Ontology (GO Annotations)
| GO ID | Source | Type | Description |
|---|---|---|---|
| GO:0016021 | IEA:UniProtKB-KW | C | integral component of membrane |
| GO:0050660 | IEA:InterPro | F | flavin adenine dinucleotide binding |
| GO:0004506 | IGI:TAIR | F | squalene monooxygenase activity |
| GO:0009414 | IMP:TAIR | P | response to water deprivation |
| GO:0016126 | IMP:TAIR | P | sterol biosynthetic process |
KEGG Pathway Links
| KEGG Pathway ID | Description |
|---|---|
| ath00909 | Sesquiterpenoid and triterpenoid biosynthesis |
| ath00100 | Steroid biosynthesis |
REACTOME Pathway Links
Domain Information
UniProt Annotations
Entry Information
Gene Name
probable squalene epoxidase 1
Protein Entry
ERG14_ARATH
UniProt ID
Species
Arabidopsis
Comments
| Comment Type | Description |
|---|---|
| Catalytic Activity | Squalene + NADPH + O(2) = (3S)-2,3-epoxy-2,3- dihydrosqualene + NADP(+) + H(2)O. {ECO:0000269|PubMed:17426032}. |
| Cofactor | Name=FAD; Xref=ChEBI:CHEBI:57692; Evidence={ECO:0000250}; |
| Disruption Phenotype | Non viable, sterile dwarf plants with short, highly branched roots. {ECO:0000269|PubMed:17426032}. |
| Function | Catalyzes the first oxygenation step in sterol biosynthesis and is suggested to be one of the rate-limiting enzymes in this pathway. Can produce not only oxidosqualene, but also 2,3:22,23-dioxidosqualene. Main squalene epoxidase in the root. Sqe1 mutants may show defects in membrane lipid rafts, impairing the correct localization of RHD2 NADPH oxidase and the proper polarized production of ROS. {ECO:0000269|PubMed:17426032, ECO:0000269|PubMed:19309460, ECO:0000269|PubMed:19847116}. |
| Miscellaneous | SEQ4 or SEQ5 are unable to complement seq1 mutants. {ECO:0000305|PubMed:17426032}. |
| Pathway | Terpene metabolism; lanosterol biosynthesis; lanosterol from farnesyl diphosphate: step 2/3. |
| Sequence Caution | Sequence=AAG50645.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305}; Sequence=AEM36345.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305}; |
| Similarity | Belongs to the squalene monooxygenase family. {ECO:0000305}. |
| Subcellular Location | Membrane {ECO:0000305}; Multi-pass membrane protein {ECO:0000305}. |
| Tissue Specificity | Expressed in seedlings, leaves, stems, inflorescences, sepals, style and siliques. Expressed in expanded cotyledons, root tips and cortical cells of the root elongation zone, but not in root hair cells. In leaves, expressed in most cells, with a very strong expression in stomata. {ECO:0000269|PubMed:17426032, ECO:0000269|PubMed:19309460}. |
Identical and Related Proteins
Unique RefSeq proteins for LMP011306 (as displayed in Record Overview)
| Protein GI | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| 18406296 | RefSeq | NP_564734 | 531 | probable squalene epoxidase 1 |
Identical Sequences to LMP011306 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:18406296 | GenBank | AAM20494.1 | 531 | squalene monooxygenase, putative [Arabidopsis thaliana] |
| GI:18406296 | GenBank | AAN15558.1 | 531 | squalene monooxygenase, putative [Arabidopsis thaliana] |
| GI:18406296 | GenBank | AEE33549.1 | 531 | probable squalene epoxidase 1 [Arabidopsis thaliana] |
| GI:18406296 | GenBank | AFX49045.1 | 531 | Sequence 50878 from patent US 8299318 |
| GI:18406296 | GenBank | AGF14292.1 | 531 | Sequence 13711 from patent US 8362325 |
| GI:18406296 | SwissProt | Q9SM02.1 | 531 | RecName: Full=Squalene epoxidase 1; Short=AtSQE1; AltName: Full=Protein DROUGHT HYPERSENSITIVE 2; AltName: Full=Squalene monooxygenase; AltName: Full=XF1 protein [Arabidopsis thaliana] |
Related Sequences to LMP011306 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:18406296 | GenBank | AAG50645.1 | 535 | squalene monooxygenase, putative [Arabidopsis thaliana] |
| GI:18406296 | GenBank | EFH62949.1 | 530 | hypothetical protein ARALYDRAFT_475389 [Arabidopsis lyrata subsp. lyrata] |
| GI:18406296 | GenBank | AEM36345.1 | 522 | At1g58440 [Arabidopsis thaliana] |
| GI:18406296 | RefSeq | XP_002886690.1 | 530 | hypothetical protein ARALYDRAFT_475389 [Arabidopsis lyrata subsp. lyrata] |
| GI:18406296 | RefSeq | XP_010511069.1 | 534 | PREDICTED: squalene epoxidase 1-like [Camelina sativa] |
| GI:18406296 | RefSeq | XP_010470253.1 | 534 | PREDICTED: squalene epoxidase 1 [Camelina sativa] |