Gene/Proteome Database (LMPD)
LMPD ID
LMP011315
Gene ID
Species
Arabidopsis thaliana (Arabidopsis)
Gene Name
probable steroid reductase DET2
Gene Symbol
Synonyms
ATDET2; DE-ETIOLATED 2; DET2; DWARF 6; DWF6; STEROID REDUCTASE DET2; T8P21.4; T8P21_4
Alternate Names
probable steroid reductase DET2
Chromosome
2
EC Number
1.3.1.22
Summary
Similar to mammalian steroid-5-alpha-reductase. Involved in the brassinolide biosynthetic pathway.
Orthologs
Proteins
| probable steroid reductase DET2 | |
|---|---|
| Refseq ID | NP_181340 |
| Protein GI | 15224430 |
| UniProt ID | Q38944 |
| mRNA ID | NM_129361 |
| Length | 262 |
| RefSeq Status | REVIEWED |
| MEEIADKTFFRYCLLTLIFAGPPTAVLLKFLQAPYGKHNRTGWGPTVSPPIAWFVMESPTLWLTLLLFPFGRHALNPKSLLLFSPYLIHYFHRTIIYPLRLFRSSFPAGKNGFPITIAALAFTFNLLNGYIQARWVSHYKDDYEDGNWFWWRFVIGMVVFITGMYINITSDRTLVRLKKENRGGYVIPRGGWFELVSCPNYFGEAIEWLGWAVMTWSWAGIGFFLYTCSNLFPRARASHKWYIAKFKEEYPKTRKAVIPFVY | |
Gene Information
Entrez Gene ID
Gene Name
probable steroid reductase DET2
Gene Symbol
Species
Arabidopsis thaliana
Gene Ontology (GO Annotations)
| GO ID | Source | Type | Description |
|---|---|---|---|
| GO:0005737 | IEA:InterPro | C | cytoplasm |
| GO:0016021 | IEA:UniProtKB-KW | C | integral component of membrane |
| GO:0003865 | IEA:InterPro | F | 3-oxo-5-alpha-steroid 4-dehydrogenase activity |
| GO:0047751 | IEA:UniProtKB-EC | F | cholestenone 5-alpha-reductase activity |
| GO:0050213 | IDA:UniProtKB | F | progesterone 5-alpha-reductase activity |
| GO:0009917 | IDA:TAIR | F | sterol 5-alpha reductase activity |
| GO:0016132 | IDA:TAIR | P | brassinosteroid biosynthetic process |
| GO:0010268 | IEP:TAIR | P | brassinosteroid homeostasis |
| GO:0055114 | IMP:UniProtKB | P | oxidation-reduction process |
KEGG Pathway Links
| KEGG Pathway ID | Description |
|---|---|
| ath01110 | Biosynthesis of secondary metabolites |
| ath00905 | Brassinosteroid biosynthesis |
| ath_M00371 | Castasterone biosynthesis, campesterol => castasterone |
REACTOME Pathway Links
| REACTOME Pathway ID | Description |
|---|---|
| 6254049 | Androgen biosynthesis |
Domain Information
UniProt Annotations
Entry Information
Gene Name
probable steroid reductase DET2
Protein Entry
DET2_ARATH
UniProt ID
Species
Arabidopsis
Comments
| Comment Type | Description |
|---|---|
| Biophysicochemical Properties | Kinetic parameters: KM=2.5 uM for testosterone {ECO:0000269|PubMed:9108014}; KM=0.4 uM for progesterone {ECO:0000269|PubMed:9108014}; Vmax=0.2 nmol/min/mg enzyme with testosterone as substrate {ECO:0000269|PubMed:9108014}; Vmax=0.5 nmol/min/mg enzyme with progesterone as substrate {ECO:0000269|PubMed:9108014}; pH dependence: Optimum pH is 6.8. {ECO:0000269|PubMed:9108014}; |
| Catalytic Activity | A 3-oxo-5-alpha-steroid + NADP(+) = a 3-oxo- Delta(4)-steroid + NADPH. {ECO:0000269|PubMed:10398719, ECO:0000269|PubMed:15993049, ECO:0000269|PubMed:9108014}. |
| Disruption Phenotype | Brassinolide-reversible dark- and light- grown defects; small dark-green dwarf plants with a limited leaves expension due to a reduced number of cells per leaf blade. {ECO:0000269|PubMed:10394910, ECO:0000269|PubMed:11867704, ECO:0000269|PubMed:8602526, ECO:0000269|PubMed:9401120}. |
| Enzyme Regulation | Inhibited by the 4-azasteroids 4-MA. {ECO:0000269|PubMed:9108014}. |
| Function | Involved in a reduction step in the biosynthesis of the plant steroid, brassinolide; acts at the second step in brassinolide biosynthesis in the 5alpha-reduction of (24R)- 24- methylcholest-4-en-3-one, which is further modified to form campestanol. Can use progesterone, testosterone, androstenedione and campestenone as substrate. {ECO:0000269|PubMed:10398719, ECO:0000269|PubMed:11867704, ECO:0000269|PubMed:15993049, ECO:0000269|PubMed:8602526, ECO:0000269|PubMed:9108014, ECO:0000269|PubMed:9401120}. |
| Pathway | Plant hormone biosynthesis; brassinosteroid biosynthesis. |
| Similarity | Belongs to the steroid 5-alpha reductase family. {ECO:0000305}. |
| Subcellular Location | Membrane {ECO:0000250}; Multi-pass membrane protein {ECO:0000250}. |
Identical and Related Proteins
Unique RefSeq proteins for LMP011315 (as displayed in Record Overview)
| Protein GI | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| 15224430 | RefSeq | NP_181340 | 262 | probable steroid reductase DET2 |
Identical Sequences to LMP011315 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:15224430 | GenBank | AAK76600.1 | 262 | putative 3-oxo-5-alpha-steroid 4-dehydrogenase [Arabidopsis thaliana] |
| GI:15224430 | GenBank | AAL85068.1 | 262 | putative 3-oxo-5-alpha-steroid 4-dehydrogenase [Arabidopsis thaliana] |
| GI:15224430 | GenBank | AEC09484.1 | 262 | probable steroid reductase DET2 [Arabidopsis thaliana] |
| GI:15224430 | SwissProt | Q38944.2 | 262 | RecName: Full=Steroid 5-alpha-reductase DET2; Short=AtDET2; AltName: Full=Protein COMPACT ROSETTE 1; AltName: Full=Protein DEETIOLATED 2 [Arabidopsis thaliana] |
Related Sequences to LMP011315 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:15224430 | GenBank | AAC49264.1 | 262 | steroid reductase DET2 [Arabidopsis thaliana] |
| GI:15224430 | GenBank | AAE95368.1 | 262 | Sequence 2 from patent US 6352846 |
| GI:15224430 | GenBank | AAE95369.1 | 246 | Sequence 3 from patent US 6352846 |
| GI:15224430 | GenBank | EFH57820.1 | 261 | predicted protein [Arabidopsis lyrata subsp. lyrata] |
| GI:15224430 | GenBank | KFK36703.1 | 262 | hypothetical protein AALP_AA4G158400 [Arabis alpina] |
| GI:15224430 | RefSeq | XP_002881561.1 | 261 | predicted protein [Arabidopsis lyrata subsp. lyrata] |