Gene/Proteome Database (LMPD)

LMPD ID
LMP011420
Gene ID
842869
Species
Arabidopsis thaliana (Arabidopsis)
Gene Name
Type II inositol-1,4,5-trisphosphate 5-phosphatase FRA3
Gene Symbol
FRA3
Synonyms
F5I14.11; F5I14_11; FRA3; FRAGILE FIBER3
Alternate Names
Type II inositol-1,4,5-trisphosphate 5-phosphatase FRA3
Chromosome
1
EC Number
3.1.3.36

Proteins

Type II inositol-1,4,5-trisphosphate 5-phosphatase FRA3
Refseq ID NP_176736
Protein GI 42562979
UniProt ID Q84W55
mRNA ID NM_105232
Length 1101
RefSeq Status REVIEWED
MEDRQNDQNDDVFSFFSPSFSAATPSTLFNRSAYSSSSSSGDDESQPSVDDSNKRIDYMIQFLDRRLSEDGNHDGIGDGNGSDSLPEFVGKCGESGIFKVPIRSAVHPNRPPSLDVRPHPLRETQIGRFLRTMTSTERQLWTGGEDGALRVWEFSELYGSGRGLEVEDTAPYKESLGNEFGSAAVVCMIGDEGSRVVWSGHRDGRIRCWRLRGDHGIEEALSWQAHRGPVLSIAISAYGDIWSGSEGGALKVWPWDGALGKSLSLKMEERHMAALAVERSYIDPRNMVSANGFANTLTSDVTFLVSDHTRARVWSASPLTFAIWDARTRDLIKVFNIDGQLENRPENSVYPDFGSEEEGKMKVTASKKEKAQSSLGFFQRSRNAIMGAADAVRRAATKGGFCDDSRKTEAIVISVDGMIWTGSSNGILMRWDGNGNCLQEFAYESSGILCMFTFCSRLWVGYSNGTVQVWDLEGKLLGGWVAHSGPVIKMAIGAGYLFTLANHGGIRGWNVTSPGPLDNVLRAELAGKEFLYSRIENLKILAGTWNVGEGRASTDSLVSWLGCAATGVEIVVVGLQEVEMGAGVLAMSAAKETVGLEGSPLGQWWLDMIGKTLDEGSSFVRVGSRQLAGLLICVWVRHDLKPHVGDVDAAAVPCGFGRAIGNKGAVGVRLRMYDRVLCFVNCHFAAHLEAVNRRNADFDHVYRTMTFSRQSSSLNAGVAGASFGVTMPRGGNALGVNTIEARPELSEADMVIFLGDFNYRLDDITYDETRDFISQRCFDWLREKDQLHTEMEAGNVFQGMREAIIRFPPTYKFERHQAGLAGYDSGEKKRIPAWCDRILYRDNKKHLGAECSLDCPVVSSISQYDACMEVTDSDHKPVRCVFSVKIARVDESVRRQEYGNIINSNKKIKVLLGELSKVPETIVSTNNIILQNQDSTILRITNKSEKNIAFFKIICEGQSKIEEDGQAHDHRARGSFGFPQWLEVSPGTGTIKPNQIAEVSVHLEDFPTVEEFVDGVAQNSWCEDTRDKEVILVLVVHGRFSTETRKHRIRVRHCPRGGPAKNHFNDGTKTSGQINALHRSDYHQLSNTLDVVEQLKNLHSP

Gene Information

Entrez Gene ID
842869
Gene Name
Type II inositol-1,4,5-trisphosphate 5-phosphatase FRA3
Gene Symbol
FRA3
Species
Arabidopsis thaliana

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0046872 IEA:UniProtKB-KW F metal ion binding
GO:0004439 IEA:UniProtKB-EC F phosphatidylinositol-4,5-bisphosphate 5-phosphatase activity
GO:0046856 IEA:InterPro P phosphatidylinositol dephosphorylation

REACTOME Pathway Links

REACTOME Pathway ID Description
6254053 Rho GTPase cycle
6254054 Signaling by Rho GTPases
6254349 Synthesis of IP2, IP, and Ins in the cytosol
6254351 Synthesis of IP3 and IP4 in the cytosol

Domain Information

InterPro Annotations

Accession Description
IPR005135 Endonuclease/exonuclease/phosphatase
IPR000300 Inositol polyphosphate-related phosphatase
IPR001680 WD40_repeat
IPR015943 WD40/YVTN repeat-like-containing domain

UniProt Annotations

Entry Information

Gene Name
Type II inositol-1,4,5-trisphosphate 5-phosphatase FRA3
Protein Entry
IP5P4_ARATH
UniProt ID
Q84W55
Species
Arabidopsis

Comments

Comment Type Description
Biophysicochemical Properties Kinetic parameters: KM=63 uM for PtdIns(4,5)P2 (at pH 7.0 and 22 degrees Celsius); KM=299 uM for PtdIns(3,4,5)P3 (at pH 7.0 and 22 degrees Celsius); KM=1040 uM for PtdIns(1,4,5)P3 (at pH 6.5 and 22 degrees Celsius); Vmax=240 pmol/min/ug enzyme with PtdIns(4,5)P2 as substrate (at pH 7.0 and 22 degrees Celsius); Vmax=365 pmol/min/ug enzyme with PtdIns(3,4,5)P3 as substrate (at pH 7.0 and 22 degrees Celsius); Vmax=504 pmol/min/ug enzyme with PtdIns(1,4,5)P3 as substrate (at pH 6.5 and 22 degrees Celsius); pH dependence: Optimum pH is 6.5 with PtdIns(1,4,5)P3 as substrate, 7.5 with PtdIns(3,4,5)P3 as substrate, and 6.5-8.5 with PtdIns(4,5)P2 as substrate.; Temperature dependence: Optimum temperature is 22 degrees Celsius with PtdIns(1,4,5)P3 as substrate, and 22-37 degrees Celsius with PtdIns(4,5)P2 and PtdIns(3,4,5)P3 as substrates.;
Catalytic Activity 1-phosphatidyl-1D-myo-inositol 4,5- bisphosphate + H(2)O = 1-phosphatidyl-1D-myo-inositol 4-phosphate + phosphate. {ECO:0000269|PubMed:15539468}.
Cofactor Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000269|PubMed:15539468};
Function Required for secondary wall synthesis and actin organization in fiber cells. Has phosphatase activity toward PtdIns(4,5)P2, PtdIns(3,4,5)P3 and Ins(1,4,5)P3. Has a higher substrate affinity toward PtdIns(4,5)P2. {ECO:0000269|PubMed:15539468}.
Sequence Caution Sequence=AAB60921.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
Similarity Belongs to the inositol 1,4,5-trisphosphate 5- phosphatase type II family. {ECO:0000305}.
Similarity Contains 6 WD repeats. {ECO:0000305}.
Tissue Specificity Predominantly expressed in interfascicular fibers and vascular bundles. Expressed in seedlings, stems, roots and flowers. Expressed at lower level in mature leaves. {ECO:0000269|PubMed:15539468}.

Identical and Related Proteins

Unique RefSeq proteins for LMP011420 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
42562979 RefSeq NP_176736 1101 Type II inositol-1,4,5-trisphosphate 5-phosphatase FRA3

Identical Sequences to LMP011420 proteins

Reference Database Accession Length Protein Name
GI:42562979 GenBank AAV87313.1 1101 type II inositol polyphosphate 5-phosphatase [Arabidopsis thaliana]
GI:42562979 GenBank AAV87317.1 1101 type II inositol polyphosphate 5-phosphatase [Arabidopsis thaliana]
GI:42562979 GenBank AEE34398.1 1101 Type II inositol-1,4,5-trisphosphate 5-phosphatase FRA3 [Arabidopsis thaliana]
GI:42562979 SwissProt Q84W55.2 1101 RecName: Full=Type II inositol 1,4,5-trisphosphate 5-phosphatase FRA3; AltName: Full=Protein FRAGILE FIBER 3 [Arabidopsis thaliana]

Related Sequences to LMP011420 proteins

Reference Database Accession Length Protein Name
GI:42562979 EMBL CAB41466.1 1101 inositol 1,4,5-trisphosphate 5-phosphatase [Arabidopsis thaliana]
GI:42562979 GenBank AAO42229.1 1101 unknown protein [Arabidopsis thaliana]
GI:42562979 GenBank EFH64685.1 1103 hypothetical protein ARALYDRAFT_475657 [Arabidopsis lyrata subsp. lyrata]
GI:42562979 RefSeq XP_002888426.1 1103 hypothetical protein ARALYDRAFT_475657 [Arabidopsis lyrata subsp. lyrata]
GI:42562979 RefSeq XP_010415096.1 1105 PREDICTED: type II inositol 1,4,5-trisphosphate 5-phosphatase FRA3 isoform X1 [Camelina sativa]
GI:42562979 RefSeq XP_010415099.1 1105 PREDICTED: type II inositol 1,4,5-trisphosphate 5-phosphatase FRA3 isoform X4 [Camelina sativa]