Gene/Proteome Database (LMPD)

LMPD ID
LMP011908
Gene ID
493602
Species
Danio rerio (Zebrafish)
Gene Name
N-acylsphingosine amidohydrolase 2
Gene Symbol
asah2
Alternate Names
neutral ceramidase; znCD; NCDase; N-CDase; non-lysosomal ceramidase; acylsphingosine deacylase 2
Chromosome
12
EC Number
3.5.1.23

Proteins

neutral ceramidase
Refseq ID NP_001007764
Protein GI 56090224
UniProt ID Q5W7F1
mRNA ID NM_001007763
Length 743
RefSeq Status PROVISIONAL
MASKSRRLSGLEISLIVLFLLMTAVSVALITVLALKQESDKKEEVTPEEPSPSVTPPEKPYLIGVGRADCTGPVADLPMMGYANTDQTARGLHTRLFSRAFIVDDGNKRVVFVTSDIGMVSQRLRLEVFQALKEKYGDLYRQDNVVLSGTHTHSGVGGYFQYTLFMITSKGYIKPSIQAIVSGIVKSIDIAHRNLRPGRIFINKGQVADSNFNRSPHSYMNNPEEERNRYEFNTDKQIVVLKFTDLDGDGIGLLSWFAVHPVSMNYTNRMVSSDNLGYASYIFEQEKNIGFLPGEKGPFVAGFSSSNLGDSSPNIRGPVCVNTGLKCDYINSSCPVGGKKACIAFGPGEDMFESTRIIGENMFKIAKELYGSAKQELHGPVYGAHQWVNMTDETVQFNSTHTGRTCKPALGHSFAAGTTDGGGEFNFLQGDTEGDPFWDGIRDAVLGPPSNETKACHQPKPILFSTGEMDSPLPWHPAIVDVQIITIGSLAVVAVPGEFTTMSGRRIREAVKRELEVKEPFTNAEVVVAGLCNIYTHYITTYEEYQIQRYEGASTIFGPHTLSAYIQRYRGLAKAIAHGTIGELPKGPEPPFFDEDKLFNQVRDPVADVAPVGTTFGDVLQEVNPVYKVGEIASVTFVSGNPRHSGDIRDTTLVTVERFHNDTGSWEIIHNDASWETRFHWIKGLAGRSQAKVEWHIPQTAQAGTYQIQYFGHYKQTTENTTVITPYVGTSAAFKVARSFYYF

Gene Information

Entrez Gene ID
493602
Gene Name
N-acylsphingosine amidohydrolase 2
Gene Symbol
asah2
Species
Danio rerio

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0000139 IDA:ZFIN C Golgi membrane
GO:0016324 IDA:ZFIN C apical plasma membrane
GO:0005789 IDA:ZFIN C endoplasmic reticulum membrane
GO:0005887 ISS:ZFIN C integral component of plasma membrane
GO:0005886 IDA:ZFIN C plasma membrane
GO:0017040 IDA:ZFIN F ceramidase activity
GO:0006672 IDA:ZFIN P ceramide metabolic process
GO:0007275 IEA:UniProtKB-KW P multicellular organismal development

KEGG Pathway Links

KEGG Pathway ID Description
dre01100 Metabolic pathways
dre00600 Sphingolipid metabolism
dre_M00099 Sphingosine biosynthesis

REACTOME Pathway Links

REACTOME Pathway ID Description
6176806 Glycosphingolipid metabolism

Domain Information

InterPro Annotations

Accession Description
IPR006823 Neutral/alkaline nonlysosomal ceramidase

UniProt Annotations

Entry Information

Gene Name
N-acylsphingosine amidohydrolase 2
Protein Entry
ASAH2_DANRE
UniProt ID
Q5W7F1
Species
Zebrafish

Comments

Comment Type Description
Catalytic Activity N-acylsphingosine + H(2)O = a carboxylate + sphingosine.
Disruption Phenotype Fishes display severe embryonic morphological and cellular abnormalities such as abnormal morphogenesis in the head and tail, pericardiac edema, defect of blood cell circulation, and an increase of apoptotic cells, especially in the head and neural tube regions, at 36 hours post- fertilization. {ECO:0000269|PubMed:15271994}.
Function Hydrolyzes the sphingolipid ceramide into sphingosine and free fatty acid at an optimal pH of 6.5-8.5. Acts as a key regulator of sphingolipid signaling metabolites by generating sphingosine at the cell surface. Essential for the metabolism of ceramide and the early development. {ECO:0000269|PubMed:15271994}.
Ptm N-glycosylated. {ECO:0000269|PubMed:15271994}.
Ptm O-glycosylated. {ECO:0000269|PubMed:15271994}.
Similarity Belongs to the neutral ceramidase family. {ECO:0000305}.
Subcellular Location Cell membrane {ECO:0000269|PubMed:15271994}; Single-pass type II membrane protein {ECO:0000269|PubMed:15271994}.

Identical and Related Proteins

Unique RefSeq proteins for LMP011908 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
56090224 RefSeq NP_001007764 743 neutral ceramidase

Identical Sequences to LMP011908 proteins

Reference Database Accession Length Protein Name
GI:56090224 DBBJ BAD69590.1 743 neutral ceramidase [Danio rerio]
GI:56090224 SwissProt Q5W7F1.1 743 RecName: Full=Neutral ceramidase; Short=N-CDase; Short=NCDase; AltName: Full=Acylsphingosine deacylase 2; AltName: Full=N-acylsphingosine amidohydrolase 2; Short=znCD [Danio rerio]

Related Sequences to LMP011908 proteins

Reference Database Accession Length Protein Name
GI:56090224 RefSeq XP_004086865.1 729 PREDICTED: neutral ceramidase-like [Oryzias latipes]
GI:56090224 RefSeq XP_004565298.1 734 PREDICTED: neutral ceramidase-like [Maylandia zebra]
GI:56090224 RefSeq XP_003449505.2 854 PREDICTED: neutral ceramidase-like [Oreochromis niloticus]
GI:56090224 RefSeq XP_005806781.1 732 PREDICTED: neutral ceramidase-like [Xiphophorus maculatus]
GI:56090224 RefSeq XP_007573621.1 737 PREDICTED: neutral ceramidase-like isoform X1 [Poecilia formosa]
GI:56090224 RefSeq XP_007544879.1 737 PREDICTED: neutral ceramidase-like isoform X1 [Poecilia formosa]