LIPID MAPS

LMPD ID

LMP012349

Gene ID

9374

Species

Homo sapiens (Human)

Gene Name

palmitoyl-protein thioesterase 2

Gene Symbol

PPT2

Synonyms

G14; PPT-2; C6orf8;

Chromosome

6

Map Location

6p21.3

EC Number

3.1.2.-

Summary

This gene encodes a member of the palmitoyl-protein thioesterase family. The encoded glycosylated lysosomal protein has palmitoyl-CoA hydrolase activity in vitro, but does not hydrolyze palmitate from cysteine residues in proteins. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the downstream EGFL8 (EGF-like-domain, multiple 8) gene. [provided by RefSeq, Feb 2011]

Orthologs

View orthologs and multiple alignments for PPT2

lysosomal thioesterase PPT2 isoform a precursor
Refseq ID	NP_005146
Protein GI	323362946
UniProt ID	Q9UMR5
mRNA ID	NM_005155
Length	302
MLGLCGQRLPAAWVLLLLPFLPLLLLAAPAPHRASYKPVIVVHGLFDSSYSFRHLLEYINETHPGTVVTVLDLFDGRESLRPLWEQVQGFREAVVPIMAKAPQGVHLICYSQGGLVCRALLSVMDDHNVDSFISLSSPQMGQYGDTDYLKWLFPTSMRSNLYRICYSPWGQEFSICNYWHDPHHDDLYLNASSFLALINGERDHPNATVWRKNFLRVGHLVLIGGPDDGVITPWQSSFFGFYDANETVLEMEEQLVYLRDSFGLKTLLARGAIVRCPMAGISHTAWHSNRTLYETCIEPWLS

lysosomal thioesterase PPT2 isoform a precursor
Refseq ID	NP_001191032
Protein GI	323362948
UniProt ID	Q9UMR5
mRNA ID	NM_001204103
Length	302
Protein sequence is identical to GI:323362946 (mRNA isoform)

lysosomal thioesterase PPT2 isoform b precursor
Refseq ID	NP_619731
Protein GI	323362944
UniProt ID	Q9UMR5
mRNA ID	NM_138717
Length	308
MKSCGSMLGLCGQRLPAAWVLLLLPFLPLLLLAAPAPHRASYKPVIVVHGLFDSSYSFRHLLEYINETHPGTVVTVLDLFDGRESLRPLWEQVQGFREAVVPIMAKAPQGVHLICYSQGGLVCRALLSVMDDHNVDSFISLSSPQMGQYGDTDYLKWLFPTSMRSNLYRICYSPWGQEFSICNYWHDPHHDDLYLNASSFLALINGERDHPNATVWRKNFLRVGHLVLIGGPDDGVITPWQSSFFGFYDANETVLEMEEQLVYLRDSFGLKTLLARGAIVRCPMAGISHTAWHSNRTLYETCIEPWLS
sig_peptide: 1..27 inference: COORDINATES: ab initio prediction:SignalP:4.0 calculated_mol_wt: 2946 peptide sequence: MLGLCGQRLPAAWVLLLLPFLPLLLLA mat_peptide: 28..302 product: Lysosomal thioesterase PPT2 experiment: experimental evidence, no additional details recorded note: propagated from UniProtKB/Swiss-Prot (Q9UMR5.4) calculated_mol_wt: 31298 peptide sequence: APAPHRASYKPVIVVHGLFDSSYSFRHLLEYINETHPGTVVTVLDLFDGRESLRPLWEQVQGFREAVVPIMAKAPQGVHLICYSQGGLVCRALLSVMDDHNVDSFISLSSPQMGQYGDTDYLKWLFPTSMRSNLYRICYSPWGQEFSICNYWHDPHHDDLYLNASSFLALINGERDHPNATVWRKNFLRVGHLVLIGGPDDGVITPWQSSFFGFYDANETVLEMEEQLVYLRDSFGLKTLLARGAIVRCPMAGISHTAWHSNRTLYETCIEPWLS sig_peptide: 1..27 inference: COORDINATES: ab initio prediction:SignalP:4.0 calculated_mol_wt: 2946 peptide sequence: MLGLCGQRLPAAWVLLLLPFLPLLLLA mat_peptide: 28..302 product: Lysosomal thioesterase PPT2 experiment: experimental evidence, no additional details recorded note: propagated from UniProtKB/Swiss-Prot (Q9UMR5.4) calculated_mol_wt: 31298 peptide sequence: APAPHRASYKPVIVVHGLFDSSYSFRHLLEYINETHPGTVVTVLDLFDGRESLRPLWEQVQGFREAVVPIMAKAPQGVHLICYSQGGLVCRALLSVMDDHNVDSFISLSSPQMGQYGDTDYLKWLFPTSMRSNLYRICYSPWGQEFSICNYWHDPHHDDLYLNASSFLALINGERDHPNATVWRKNFLRVGHLVLIGGPDDGVITPWQSSFFGFYDANETVLEMEEQLVYLRDSFGLKTLLARGAIVRCPMAGISHTAWHSNRTLYETCIEPWLS sig_peptide: 1..33 inference: COORDINATES: ab initio prediction:SignalP:4.0 calculated_mol_wt: 3540 peptide sequence: MKSCGSMLGLCGQRLPAAWVLLLLPFLPLLLLA mat_peptide: 34..308 product: lysosomal thioesterase PPT2 isoform b calculated_mol_wt: 31298 peptide sequence: APAPHRASYKPVIVVHGLFDSSYSFRHLLEYINETHPGTVVTVLDLFDGRESLRPLWEQVQGFREAVVPIMAKAPQGVHLICYSQGGLVCRALLSVMDDHNVDSFISLSSPQMGQYGDTDYLKWLFPTSMRSNLYRICYSPWGQEFSICNYWHDPHHDDLYLNASSFLALINGERDHPNATVWRKNFLRVGHLVLIGGPDDGVITPWQSSFFGFYDANETVLEMEEQLVYLRDSFGLKTLLARGAIVRCPMAGISHTAWHSNRTLYETCIEPWLS

Entrez Gene ID

9374

Gene Name

palmitoyl-protein thioesterase 2

Gene Symbol

PPT2

Species

Homo sapiens

Gene Ontology (GO Annotations)

GO ID	Source	Type	Description
GO:0070062	IDA:UniProtKB	C	extracellular vesicular exosome
GO:0005764	NAS:UniProtKB	C	lysosome
GO:0098599	IDA:UniProtKB	F	palmitoyl hydrolase activity
GO:0008474	IEA:Ensembl	F	palmitoyl-(protein) hydrolase activity
GO:0016790	NAS:UniProtKB	F	thiolester hydrolase activity
GO:0006464	IEA:InterPro	P	cellular protein modification process
GO:0098734	IDA:GOC	P	macromolecule depalmitoylation

KEGG Pathway Links

KEGG Pathway ID	Description
hsa00062	Fatty acid elongation
ko00062	Fatty acid elongation
hsa01212	Fatty acid metabolism
ko01212	Fatty acid metabolism
hsa04142	Lysosome
ko04142	Lysosome
hsa01100	Metabolic pathways

InterPro Annotations

Accession	Description
IPR029058	Alpha/Beta hydrolase fold
IPR002472	Palmitoyl protein thioesterase

Entry Information

Gene Name

palmitoyl-protein thioesterase 2

UniProt ID

Q9UMR5

Species

Human

Comments

Comment Type	Description
Alternative Products	Event=Alternative splicing; Named isoforms=3; Name=1; IsoId=Q9UMR5-1; Sequence=Displayed; Name=2; Synonyms=I; IsoId=Q9UMR5-2; Sequence=VSP_005188; Note=Catalytically inactive due to lack of His-283. May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.; Name=3; IsoId=Q9UMR5-3; Sequence=VSP_054027; Note=No experimental confirmation available.;
Biophysicochemical Properties	Kinetic parameters: KM=67 uM for S-palmitoyl-CoA ; KM=37 uM for S-palmitoyl-N-acetylcysteamine ; KM=117 uM for 4-methylumbelliferyl-6-S-palmitoyl-beta-D- glucopyranoside ; Vmax=1.7 umol/min/mg enzyme toward S-palmitoyl-CoA ; Vmax=3.3 umol/min/mg enzyme toward S-palmitoyl-N- acetylcysteamine ; Vmax=0.43 umol/min/mg enzyme toward 4-methylumbelliferyl-6-S- palmitoyl-beta-D-glucopyranoside ; pH dependence: Optimum pH is 7.0. ;
Caution	Was originally referred as a palmitoyl-protein thioesterase (palmitoyl-protein hydrolase)
Function	Removes thioester-linked fatty acyl groups from various substrates including S-palmitoyl-CoA. Has the highest S- thioesterase activity for the acyl groups palmitic and myristic acid followed by other short- and long-chain acyl substrates. However, because of structural constraints, is unable to remove palmitate from peptides or proteins. {ECO:0000269\|PubMed:10417332, ECO:0000269\|PubMed:12855696, ECO:0000269\|PubMed:9341199}.
Sequence Caution	Sequence=CAG38577.1; Type=Erroneous initiation; Note=Translation N-terminally shortened.; Evidence= ;
Similarity	Belongs to the palmitoyl-protein thioesterase family
Subcellular Location	Lysosome
Tissue Specificity	Broadly expressed, with highest levels in skeletal muscle