Gene/Proteome Database (LMPD)
LMPD ID
LMP012386
Gene ID
Species
Rattus norvegicus (Rat)
Gene Name
acyl-CoA dehydrogenase, C-4 to C-12 straight chain
Gene Symbol
Synonyms
MCAD;
Alternate Names
medium-chain specific acyl-CoA dehydrogenase, mitochondrial; medium-chain acyl-CoA dehydrogenase; acyl-Coenzyme A dehydrogenase, medium chain; Acyl-Coenzyme A dehydrogenase C-4 to C-12 straight-chain; Acyl-Coenzyme A dehydrogenase, C-4 to C-12 straight-chain; acyl-Coenzyme A dehydrogenase, C-4 to C-12 straight chain;
Chromosome
2
Map Location
2q45
EC Number
1.3.8.7
Summary
catalyzes the alpha, beta-dehydrogenation of acyl-CoA esters in fatty acid metabolism [RGD, Feb 2006]
Orthologs
Proteins
| medium-chain specific acyl-CoA dehydrogenase, mitochondrial precursor | |
|---|---|
| Refseq ID | NP_058682 |
| Protein GI | 292494885 |
| UniProt ID | P08503 |
| mRNA ID | NM_016986 |
| Length | 421 |
| MAAALRRGYKVLRSVSHFECRAQHTKPSLKQEPGLGFSFELTEQQKEFQTIARKFAREEIIPVAPDYDKSGEYPFPLIKRAWELGLINTHIPESCGGLGLGTFDACLITEELAYGCTGVQTAIEANSLGQMPVIIAGNDQQKKKYLGRMTEQLMMCAYCVTEPSAGSDVAGIKTKAEKKGDEYVINGQKMWITNGGKANWYFVLTRSNPDPKVPASKAFTGFIVEADTPGIHIGKKELNMGQRCSDTRGITFEDVRVPKENVLIGEGAGFKIAMGAFDRTRPTVAAGAVGLAQRALDEATKYALDRKTFGKLLVEHQGVSFLLAEMAMKVELARLSYQRAAWEVDSGRRNTYFASIAKAFAGDIANQLATDAVQIFGGYGFNTEYPVEKLMRDAKIYQIYEGTAQIQRLIIAREHIEKYKN | |
| transit_peptide: 1..25 calculated_mol_wt: 2888 peptide sequence: MAAALRRGYKVLRSVSHFECRAQHT mat_peptide: 26..421 product: medium-chain specific acyl-CoA dehydrogenase, mitochondrial calculated_mol_wt: 43701 peptide sequence: KPSLKQEPGLGFSFELTEQQKEFQTIARKFAREEIIPVAPDYDKSGEYPFPLIKRAWELGLINTHIPESCGGLGLGTFDACLITEELAYGCTGVQTAIEANSLGQMPVIIAGNDQQKKKYLGRMTEQLMMCAYCVTEPSAGSDVAGIKTKAEKKGDEYVINGQKMWITNGGKANWYFVLTRSNPDPKVPASKAFTGFIVEADTPGIHIGKKELNMGQRCSDTRGITFEDVRVPKENVLIGEGAGFKIAMGAFDRTRPTVAAGAVGLAQRALDEATKYALDRKTFGKLLVEHQGVSFLLAEMAMKVELARLSYQRAAWEVDSGRRNTYFASIAKAFAGDIANQLATDAVQIFGGYGFNTEYPVEKLMRDAKIYQIYEGTAQIQRLIIAREHIEKYKN | |
Gene Information
Entrez Gene ID
Gene Name
acyl-CoA dehydrogenase, C-4 to C-12 straight chain
Gene Symbol
Species
Rattus norvegicus
Gene Ontology (GO Annotations)
| GO ID | Source | Type | Description |
|---|---|---|---|
| GO:0005759 | IDA:BHF-UCL | C | mitochondrial matrix |
| GO:0031966 | IDA:BHF-UCL | C | mitochondrial membrane |
| GO:0005739 | IDA:RGD | C | mitochondrion |
| GO:0003995 | ISS:UniProtKB | F | acyl-CoA dehydrogenase activity |
| GO:0000062 | IDA:RGD | F | fatty-acyl-CoA binding |
| GO:0050660 | IDA:RGD | F | flavin adenine dinucleotide binding |
| GO:0042802 | IDA:BHF-UCL | F | identical protein binding |
| GO:0016853 | IDA:RGD | F | isomerase activity |
| GO:0006635 | ISS:UniProtKB | P | fatty acid beta-oxidation |
| GO:0033539 | IDA:RGD | P | fatty acid beta-oxidation using acyl-CoA dehydrogenase |
| GO:0051793 | IDA:RGD | P | medium-chain fatty acid catabolic process |
| GO:0051289 | IDA:RGD | P | protein homotetramerization |
| GO:0046688 | IEP:RGD | P | response to copper ion |
| GO:0042493 | IEP:RGD | P | response to drug |
| GO:0051384 | IEP:RGD | P | response to glucocorticoid |
| GO:0009725 | IEP:RGD | P | response to hormone |
| GO:0007584 | IEP:RGD | P | response to nutrient |
KEGG Pathway Links
| KEGG Pathway ID | Description |
|---|---|
| ko01200 | Carbon metabolism |
| rno01200 | Carbon metabolism |
| ko00071 | Fatty acid degradation |
| rno00071 | Fatty acid degradation |
| ko01212 | Fatty acid metabolism |
| rno01212 | Fatty acid metabolism |
| M00036 | Leucine degradation, leucine => acetoacetate + acetyl-CoA |
| M00013 | Malonate semialdehyde pathway, propanoyl-CoA => Acetyl-CoA |
| rno01100 | Metabolic pathways |
| ko03320 | PPAR signaling pathway |
| rno03320 | PPAR signaling pathway |
| ko00640 | Propanoate metabolism |
| rno00640 | Propanoate metabolism |
| ko00280 | Valine, leucine and isoleucine degradation |
| rno00280 | Valine, leucine and isoleucine degradation |
| ko00410 | beta-Alanine metabolism |
| rno00410 | beta-Alanine metabolism |
| M00087 | beta-Oxidation |
REACTOME Pathway Links
| REACTOME Pathway ID | Description |
|---|---|
| 5953503 | Beta oxidation of decanoyl-CoA to octanoyl-CoA-CoA |
| 5953502 | Beta oxidation of octanoyl-CoA to hexanoyl-CoA |
| 5953288 | Fatty acid, triacylglycerol, and ketone body metabolism |
| 5953250 | Metabolism |
| 5953289 | Metabolism of lipids and lipoproteins |
| 5953287 | Mitochondrial Fatty Acid Beta-Oxidation |
| 5954333 | PPARA activates gene expression |
| 5954222 | Regulation of lipid metabolism by Peroxisome proliferator-activated receptor alpha (PPARalpha) |
| 5953497 | mitochondrial fatty acid beta-oxidation of saturated fatty acids |
| 5953518 | mitochondrial fatty acid beta-oxidation of unsaturated fatty acids |
Domain Information
InterPro Annotations
| Accession | Description |
|---|---|
| IPR006089 | Acyl-CoA dehydrogenase, conserved site |
| IPR009075 | Acyl-CoA dehydrogenase/oxidase C-terminal |
| IPR013786 | Acyl-CoA dehydrogenase/oxidase, N-terminal |
| IPR009100 | Acyl-CoA dehydrogenase/oxidase, N-terminal and middle domain |
| IPR006091 | Acyl-CoA oxidase/dehydrogenase, central domain |
UniProt Annotations
Entry Information
Gene Name
acyl-CoA dehydrogenase, C-4 to C-12 straight chain
Protein Entry
ACADM_RAT
UniProt ID
Species
Rat
Comments
| Comment Type | Description |
|---|---|
| Catalytic Activity | A medium-chain acyl-CoA + electron-transfer flavoprotein = a medium-chain trans-2,3-dehydroacyl-CoA + reduced electron-transfer flavoprotein. |
| Cofactor | Name=FAD; Xref=ChEBI:CHEBI:57692; |
| Function | This enzyme is specific for acyl chain lengths of 4 to 16. |
| Miscellaneous | A number of straight-chain acyl-CoA dehydrogenases of different substrate specificities are present in mammalian tissues. |
| Pathway | Lipid metabolism; mitochondrial fatty acid beta- oxidation. |
| Ptm | Acetylation at Lys-307 and Lys-311 in proximity of the cofactor-binding sites reduces catalytic activity. These sites are deacetylated by SIRT3 (By similarity) |
| Similarity | Belongs to the acyl-CoA dehydrogenase family |
| Subcellular Location | Mitochondrion matrix. |
| Subunit | Homotetramer. |
Identical and Related Proteins
Unique RefSeq proteins for LMP012386 (as displayed in Record Overview)
| Protein GI | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| 292494885 | RefSeq | NP_058682 | 421 | medium-chain specific acyl-CoA dehydrogenase, mitochondrial precursor |
Identical Sequences to LMP012386 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:292494885 | GenBank | EDL82532.1 | 421 | acetyl-Coenzyme A dehydrogenase, medium chain [Rattus norvegicus] |
Related Sequences to LMP012386 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:292494885 | GenBank | AAA40670.1 | 421 | acyl-CoA dehydrogenase medium subunit (EC 1.3.99.3) [Rattus norvegicus] |
| GI:292494885 | GenBank | EDL82532.1 | 421 | acetyl-Coenzyme A dehydrogenase, medium chain [Rattus norvegicus] |
| GI:292494885 | SwissProt | P08503.1 | 421 | RecName: Full=Medium-chain specific acyl-CoA dehydrogenase, mitochondrial; Short=MCAD; Flags: Precursor [Rattus norvegicus] |