Gene/Proteome Database (LMPD)

LMPD ID
LMP012425
Gene ID
24680
Species
Rattus norvegicus (Rat)
Gene Name
protein kinase C, alpha
Gene Symbol
Prkca
Synonyms
Pkca;
Alternate Names
protein kinase C alpha type; PKC-A; PKC-alpha;
Chromosome
10
Map Location
10q32.1
Summary
displays Ca+2 dependent protein kinase activity [RGD, Feb 2006]
Orthologs
View orthologs and multiple alignments for Prkca

Proteins

protein kinase C alpha type
Refseq ID NP_001099183
Protein GI 157786690
mRNA ID NM_001105713
Length 636
MADVYPANDSTASQDVANRFARKGALRQKNVHEVKDHKFIARFFKQPTFCSHCTDFIWGFGKQGFQCQVCCFVVHKRCHEFVTFSCPGADKGPDTDDPRSKHKFKIHTYGSPTFCDHCGSLLYGLIHQGMKCDTCDMNVHKQCVINVPSLCGMDHTEKRGRIYLKAEVTDEKLHVTVRDAKNLIPMDPNGLSDPYVKLKLIPDPKNESKQKTKTIRSTLNPQWNESFTFKLKPSDKDRRLSVEIWDWDRTTRNDFMGSLSFGVSELMKMPASGWYKLLNQEEGEYYNVPIPEGDEEGNVELRQKFEKAKLGPAGNKVISPSEDRKQPSNNLDRVKLTDFNFLMVLGKGSFGKVMLADRKGTEELYAIKILKKDVVIQDDDVECTMVEKRVLALLDKPPFLTQLHSCFQTVDRLYFVMEYVNGGDLMYHIQQVGKFKEPQAVFYAAEISIGLFFLHKRGIIYRDLKLDNVMLDSEGHIKIADFGMCKEHMMDGVTTRTFCGTPDYIAPEIIAYQPYGKSVDWWAYGVLLYEMLAGQLMTKHPAKRLGCGPEGERDVREHAFFRRIDWEKLENREIQPPFKPKVCGKGAENFDKFFTRGQPVLTPPDQLVIANIDQSDFEGFSYVNPQFVHPILQSAV

Gene Information

Entrez Gene ID
24680
Gene Name
protein kinase C, alpha
Gene Symbol
Prkca
Species
Rattus norvegicus

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005737 ISS:UniProtKB C cytoplasm
GO:0005829 IDA:UniProtKB C cytosol
GO:0045121 IDA:RGD C membrane raft
GO:0005739 IDA:RGD C mitochondrion
GO:0043005 IDA:RGD C neuron projection
GO:0005634 IEA:UniProtKB-KW C nucleus
GO:0048471 IDA:UniProtKB C perinuclear region of cytoplasm
GO:0005886 IDA:RGD C plasma membrane
GO:0043234 IDA:RGD C protein complex
GO:0005524 IEA:UniProtKB-KW F ATP binding
GO:0004698 IDA:RGD F calcium-dependent protein kinase C activity
GO:0004697 IDA:RGD F protein kinase C activity
GO:0008270 IEA:InterPro F zinc ion binding
GO:0007568 IEP:RGD P aging
GO:0001525 IEA:UniProtKB-KW P angiogenesis
GO:0006915 IEA:UniProtKB-KW P apoptotic process
GO:0007155 IEA:UniProtKB-KW P cell adhesion
GO:0021955 IMP:RGD P central nervous system neuron axonogenesis
GO:0045184 TAS:UniProtKB P establishment of protein localization
GO:0035556 IDA:RGD P intracellular signal transduction
GO:0007611 IMP:RGD P learning or memory
GO:0034351 ISS:UniProtKB P negative regulation of glial cell apoptotic process
GO:0045822 IMP:RGD P negative regulation of heart contraction
GO:0017148 IMP:RGD P negative regulation of translation
GO:0018105 IDA:RGD P peptidyl-serine phosphorylation
GO:0018107 IDA:RGD P peptidyl-threonine phosphorylation
GO:0070374 IMP:UniProtKB P positive regulation of ERK1 and ERK2 cascade
GO:0045766 ISS:UniProtKB P positive regulation of angiogenesis
GO:0010613 IDA:UniProtKB P positive regulation of cardiac muscle hypertrophy
GO:0045785 ISS:UniProtKB P positive regulation of cell adhesion
GO:0030335 ISS:UniProtKB P positive regulation of cell migration
GO:2000707 ISS:UniProtKB P positive regulation of dense core granule biogenesis
GO:0010595 ISS:UniProtKB P positive regulation of endothelial cell migration
GO:0001938 ISS:UniProtKB P positive regulation of endothelial cell proliferation
GO:0045921 IMP:RGD P positive regulation of exocytosis
GO:0031666 ISS:UniProtKB P positive regulation of lipopolysaccharide-mediated signaling pathway
GO:0045651 ISS:UniProtKB P positive regulation of macrophage differentiation
GO:0045931 ISS:UniProtKB P positive regulation of mitotic cell cycle
GO:0048661 IMP:RGD P positive regulation of smooth muscle cell proliferation
GO:0051965 IMP:RGD P positive regulation of synapse assembly
GO:0046777 IDA:RGD P protein autophosphorylation
GO:0006468 IDA:RGD P protein phosphorylation
GO:0090330 ISS:UniProtKB P regulation of platelet aggregation
GO:0048259 IMP:RGD P regulation of receptor-mediated endocytosis
GO:0046677 IEP:RGD P response to antibiotic
GO:0051412 IEP:RGD P response to corticosterone
GO:0032355 IMP:RGD P response to estradiol
GO:0045471 IMP:RGD P response to ethanol
GO:0009612 IEP:RGD P response to mechanical stimulus
GO:0014070 IEP:RGD P response to organic cyclic compound
GO:0043434 IEP:RGD P response to peptide hormone
GO:0000302 IMP:RGD P response to reactive oxygen species
GO:0009636 IEP:RGD P response to toxic substance

KEGG Pathway Links

KEGG Pathway ID Description
ko04261 Adrenergic signaling in cardiomyocytes
rno04261 Adrenergic signaling in cardiomyocytes
ko05143 African trypanosomiasis
rno05143 African trypanosomiasis
ko04960 Aldosterone-regulated sodium reabsorption
rno04960 Aldosterone-regulated sodium reabsorption
ko05146 Amoebiasis
rno05146 Amoebiasis
ko05031 Amphetamine addiction
rno05031 Amphetamine addiction
ko04020 Calcium signaling pathway
rno04020 Calcium signaling pathway
rno04725 Cholinergic synapse
ko04713 Circadian entrainment
rno04713 Circadian entrainment
ko04728 Dopaminergic synapse
rno04728 Dopaminergic synapse
ko04961 Endocrine and other factor-regulated calcium reabsorption
rno04961 Endocrine and other factor-regulated calcium reabsorption
ko04012 ErbB signaling pathway
rno04012 ErbB signaling pathway
ko04664 Fc epsilon RI signaling pathway
rno04664 Fc epsilon RI signaling pathway
ko04666 Fc gamma R-mediated phagocytosis
rno04666 Fc gamma R-mediated phagocytosis
ko04510 Focal adhesion
rno04510 Focal adhesion
ko04727 GABAergic synapse
rno04727 GABAergic synapse
ko04540 Gap junction
rno04540 Gap junction
ko04971 Gastric acid secretion
rno04971 Gastric acid secretion
ko05214 Glioma
rno05214 Glioma
ko04724 Glutamatergic synapse
rno04724 Glutamatergic synapse
ko04912 GnRH signaling pathway
rno04912 GnRH signaling pathway
rno04066 HIF-1 signaling pathway
rno05161 Hepatitis B
ko04750 Inflammatory mediator regulation of TRP channels
rno04750 Inflammatory mediator regulation of TRP channels
ko05164 Influenza A
rno05164 Influenza A
rno04911 Insulin secretion
ko04670 Leukocyte transendothelial migration
rno04670 Leukocyte transendothelial migration
ko04730 Long-term depression
rno04730 Long-term depression
ko04720 Long-term potentiation
rno04720 Long-term potentiation
ko04010 MAPK signaling pathway
rno04010 MAPK signaling pathway
ko04916 Melanogenesis
rno04916 Melanogenesis
ko05206 MicroRNAs in cancer
rno05206 MicroRNAs in cancer
ko05032 Morphine addiction
rno05032 Morphine addiction
ko04650 Natural killer cell mediated cytotoxicity
rno04650 Natural killer cell mediated cytotoxicity
ko05223 Non-small cell lung cancer
rno05223 Non-small cell lung cancer
ko04921 Oxytocin signaling pathway
rno04921 Oxytocin signaling pathway
ko04151 PI3K-Akt signaling pathway
rno04151 PI3K-Akt signaling pathway
ko04972 Pancreatic secretion
rno04972 Pancreatic secretion
rno05200 Pathways in cancer
ko04070 Phosphatidylinositol signaling system
rno04070 Phosphatidylinositol signaling system
ko05205 Proteoglycans in cancer
rno05205 Proteoglycans in cancer
ko04015 Rap1 signaling pathway
rno04015 Rap1 signaling pathway
rno04014 Ras signaling pathway
ko04723 Retrograde endocannabinoid signaling
rno04723 Retrograde endocannabinoid signaling
ko04970 Salivary secretion
rno04970 Salivary secretion
rno04726 Serotonergic synapse
rno04919 Thyroid hormone signaling pathway
ko04918 Thyroid hormone synthesis
rno04918 Thyroid hormone synthesis
ko04530 Tight junction
rno04530 Tight junction
ko04370 VEGF signaling pathway
rno04370 VEGF signaling pathway
ko04270 Vascular smooth muscle contraction
rno04270 Vascular smooth muscle contraction
ko04310 Wnt signaling pathway
rno04310 Wnt signaling pathway
ko04150 mTOR signaling pathway
rno04150 mTOR signaling pathway

Domain Information

InterPro Annotations

Accession Description
IPR000961 AGC-kinase, C-terminal
IPR000008 C2 domain
IPR020454 Diacylglycerol/phorbol-ester binding
IPR014375 Protein kinase C, alpha/beta/gamma types
IPR002219 Protein kinase C-like, phorbol ester/diacylglycerol-binding domain
IPR000719 Protein kinase domain
IPR017441 Protein kinase, ATP binding site
IPR017892 Protein kinase, C-terminal
IPR011009 Protein kinase-like domain
IPR008271 Serine/threonine-protein kinase, active site
IPR002290 Serine/threonine/dual specificity protein kinase, catalytic domain

UniProt Annotations

Entry Information

Gene Name
protein kinase C, alpha
Species
Rat

Comments

Comment Type Description
Catalytic Activity ATP + a protein = ADP + a phosphoprotein.
Cofactor Name=Ca(2+); Xref=ChEBI:CHEBI:29108; Evidence={ECO:0000250}; Note=Binds 3 Ca(2+) ions per subunit. The ions are bound to the C2 domain. {ECO:0000250};
Enzyme Regulation Classical (or conventional) PKCs (PRKCA, PRKCB and PRKCG) are activated by calcium and diacylglycerol (DAG) in the presence of phosphatidylserine. Three specific sites; Thr-497 (activation loop of the kinase domain), Thr-638 (turn motif) and Ser-657 (hydrophobic region), need to be phosphorylated for its full activation.
Function Calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that is involved in positive and negative regulation of cell proliferation, apoptosis, differentiation, migration and adhesion, cardiac hypertrophy, angiogenesis, platelet function and inflammation, by directly phosphorylating targets such as RAF1, BCL2, CSPG4, TNNT2/CTNT, or activating signaling cascades involving MAPK1/3 (ERK1/2) and RAP1GAP. Depending on the cell type, is involved in cell proliferation and cell growth arrest by positive and negative regulation of the cell cycle. Can promote cell growth by phosphorylating and activating RAF1, which mediates the activation of the MAPK/ERK signaling cascade, and/or by up-regulating CDKN1A, which facilitates active cyclin-dependent kinase (CDK) complex formation. In cells stimulated by the phorbol ester PMA, can trigger a cell cycle arrest program which is associated with the accumulation of the hyper-phosphorylated growth-suppressive form of RB1 and induction of the CDK inhibitors CDKN1A and CDKN1B. Depending on the cell type, exhibits anti-apoptotic function and protects cells from apoptosis by suppressing the p53/TP53-mediated activation of IGFBP3, or mediates anti-apoptotic action by phosphorylating BCL2. During macrophage differentiation induced by macrophage colony-stimulating factor (CSF1), is translocated to the nucleus and is associated with macrophage development. After wounding, translocates from focal contacts to lamellipodia and participates in the modulation of desmosomal adhesion. Plays a role in cell motility by phosphorylating CSPG4, which induces association of CSPG4 with extensive lamellipodia at the cell periphery and polarization of the cell accompanied by increases in cell motility. Negatively regulates myocardial contractility and positively regulates angiogenesis, platelet aggregation and thrombus formation in arteries. Mediates hypertrophic growth of neonatal cardiomyocytes, in part through a MAPK1/3 (ERK1/2)- dependent signaling pathway, and upon PMA treatment, is required to induce cardiomyocyte hypertrophy up to heart failure and death, by increasing protein synthesis, protein-DNA ratio and cell surface area. Regulates cardiomyocyte function by phosphorylating cardiac troponin T (TNNT2/CTNT), which induces significant reduction in actomyosin ATPase activity, myofilament calcium sensitivity and myocardial contractility. In angiogenesis, is required for full endothelial cell migration, adhesion to vitronectin (VTN), and vascular endothelial growth factor A (VEGFA)-dependent regulation of kinase activation and vascular tube formation. Involved in the stabilization of VEGFA mRNA at post-transcriptional level and mediates VEGFA-induced cell proliferation. In the regulation of calcium-induced platelet aggregation, mediates signals from the CD36/GP4 receptor for granule release, and activates the integrin heterodimer ITGA2B- ITGB3 through the RAP1GAP pathway for adhesion. During response to lipopolysaccharides (LPS), may regulate selective LPS-induced macrophage functions involved in host defense and inflammation. But in some inflammatory responses, may negatively regulate NF- kappa-B-induced genes, through IL1A-dependent induction of NF- kappa-B inhibitor alpha (NFKBIA/IKBA). Upon stimulation with 12-O- tetradecanoylphorbol-13-acetate (TPA), phosphorylates EIF4G1, which modulates EIF4G1 binding to MKNK1 and may be involved in the regulation of EIF4E phosphorylation. Phosphorylates KIT, leading to inhibition of KIT activity. Phosphorylates ATF2 which promotes cooperation between ATF2 and JUN, activating transcription. {ECO:0000269|PubMed:11076962, ECO:0000269|PubMed:11864993, ECO:0000269|PubMed:15271671, ECO:0000269|PubMed:19176525}.
Interaction P34901:Sdc4; NbExp=3; IntAct=EBI-935801, EBI-1173182;
Similarity Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily. {ECO:0000305}.
Similarity Contains 1 AGC-kinase C-terminal domain. {ECO:0000305}.
Similarity Contains 1 C2 domain. {ECO:0000255|PROSITE- ProRule:PRU00041}.
Similarity Contains 1 protein kinase domain. {ECO:0000255|PROSITE-ProRule:PRU00159}.
Similarity Contains 2 phorbol-ester/DAG-type zinc fingers. {ECO:0000255|PROSITE-ProRule:PRU00226}.
Subcellular Location Cytoplasm. Cell membrane; Peripheral membrane protein. Mitochondrion membrane {ECO:0000250}; Peripheral membrane protein {ECO:0000250}. Nucleus. Note=Translocated to the nucleus upon treatment with PMA or IGF1.
Subunit Interacts with ADAP1/CENTA1, CSPG4 and PRKCABP. Binds to SDPR in the presence of phosphatidylserine (By similarity). Interacts with PICK1 (via PDZ domain) (By similarity). Interacts with TRIM41 (By similarity). Recruited in a circadian manner into a nuclear complex which also includes BMAL1 and GNB2L1/RACK1 (By similarity). {ECO:0000250}.

Identical and Related Proteins

Unique RefSeq proteins for LMP012425 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
157786690 RefSeq NP_001099183 636 protein kinase C alpha type

Identical Sequences to LMP012425 proteins

Reference Database Accession Length Protein Name
GI:157786690 GenBank EDM06446.1 636 protein kinase C, alpha [Rattus norvegicus]

Related Sequences to LMP012425 proteins

Reference Database Accession Length Protein Name
GI:157786690 GenBank EDM06446.1 636 protein kinase C, alpha [Rattus norvegicus]
GI:157786690 GenBank AAI69007.1 672 Prkca protein [Rattus norvegicus]
GI:157786690 SwissProt P05696.3 672 RecName: Full=Protein kinase C alpha type; Short=PKC-A; Short=PKC-alpha [Rattus norvegicus]