Gene/Proteome Database (LMPD)
LMPD ID
LMP012456
Gene ID
Species
Rattus norvegicus (Rat)
Gene Name
protein kinase C, beta
Gene Symbol
Synonyms
Pkcb; Prkcb1;
Alternate Names
protein kinase C beta type; PKC-beta; protein kinase C beta I; protein kinase C beta II; protein kinase C, beta 1;
Chromosome
1
Map Location
1q36
EC Number
2.7.11.13;
Summary
plays a role in hexosamine biosynthesis pathway induced transcriptional regulation [RGD, Feb 2006]
Orthologs
Proteins
| protein kinase C beta type isoform 1 | |
|---|---|
| Refseq ID | NP_036845 |
| Protein GI | 288806592 |
| UniProt ID | P68403 |
| mRNA ID | NM_012713 |
| Length | 673 |
| MADPAAGPPPSEGEESTVRFARKGALRQKNVHEVKNHKFTARFFKQPTFCSHCTDFIWGLGLQGFQSQVCCFVVHKRCHEFVTFSCPGADKGPASDDPRSKHKFKIHTYSSPTFCDHCGSLLYGLIHQGMKCDTCMMNVHKRCVMNVPSLCGTDHTERRGRIYIQAHIDREVLIVVVRDAKNLVPMDPNGLSDPYVKLKLIPDPKSESKQKTKTIKCSLNPEWNETFRFQLKESDKDRRLSVEIWDWDLTSRNDFMGSLSFGISELQKAGVDGWFKLLSQEEGEYFNVPVPPEGSEGNEELRQKFERAKIGQGTKAPEEKTANTISKFDNNGNRDRMKLTDFNFLMVLGKGSFGKVMLSERKGTDELYAVKILKKDVVIQDDDVECTMVEKRVLALPGKPPFLTQLHSCFQTMDRLYFVMEYVNGGDLMYHIQQVGRFKEPHAVFYAAEIAIGLFFLQSKGIIYRDLKLDNVMLDSEGHIKIADFGMCKENIWDGVTTKTFCGTPDYIAPEIIAYQPYGKSVDWWAFGVLLYEMLAGQAPFEGEDEDELFQSIMEHNVAYPKSMSKEAVAICKGLMTKHPGKRLGCGPEGERDIKEHAFFRYIDWEKLERKEIQPPYKPKACGRNAENFDRFFTRHPPVLTPPDQEVIRNIDQSEFEGFSFVNSEFLKPEVKS | |
| protein kinase C beta type isoform 2 | |
|---|---|
| Refseq ID | NP_001165776 |
| Protein GI | 288806594 |
| UniProt ID | P68403 |
| mRNA ID | NM_001172305 |
| Length | 671 |
| MADPAAGPPPSEGEESTVRFARKGALRQKNVHEVKNHKFTARFFKQPTFCSHCTDFIWGLGLQGFQSQVCCFVVHKRCHEFVTFSCPGADKGPASDDPRSKHKFKIHTYSSPTFCDHCGSLLYGLIHQGMKCDTCMMNVHKRCVMNVPSLCGTDHTERRGRIYIQAHIDREVLIVVVRDAKNLVPMDPNGLSDPYVKLKLIPDPKSESKQKTKTIKCSLNPEWNETFRFQLKESDKDRRLSVEIWDWDLTSRNDFMGSLSFGISELQKAGVDGWFKLLSQEEGEYFNVPVPPEGSEGNEELRQKFERAKIGQGTKAPEEKTANTISKFDNNGNRDRMKLTDFNFLMVLGKGSFGKVMLSERKGTDELYAVKILKKDVVIQDDDVECTMVEKRVLALPGKPPFLTQLHSCFQTMDRLYFVMEYVNGGDLMYHIQQVGRFKEPHAVFYAAEIAIGLFFLQSKGIIYRDLKLDNVMLDSEGHIKIADFGMCKENIWDGVTTKTFCGTPDYIAPEIIAYQPYGKSVDWWAFGVLLYEMLAGQAPFEGEDEDELFQSIMEHNVAYPKSMSKEAVAICKGLMTKHPGKRLGCGPEGERDIKEHAFFRYIDWEKLERKEIQPPYKPKARDKRDTSNFDKEFTRQPVELTPTDKLFIMNLDQNEFAGFSYTNPEFVINV | |
Gene Information
Gene Ontology (GO Annotations)
| GO ID | Source | Type | Description |
|---|---|---|---|
| GO:0031526 | IDA:RGD | C | brush border membrane |
| GO:0005813 | IDA:RGD | C | centrosome |
| GO:0005829 | IDA:RGD | C | cytosol |
| GO:0005634 | ISS:UniProtKB | C | nucleus |
| GO:0005886 | IDA:RGD | C | plasma membrane |
| GO:0005524 | IEA:UniProtKB-KW | F | ATP binding |
| GO:0050681 | ISS:UniProtKB | F | androgen receptor binding |
| GO:0003682 | ISS:UniProtKB | F | chromatin binding |
| GO:0042393 | ISS:UniProtKB | F | histone binding |
| GO:0035403 | ISS:UniProtKB | F | histone kinase activity (H3-T6 specific) |
| GO:0030374 | ISS:UniProtKB | F | ligand-dependent nuclear receptor transcription coactivator activity |
| GO:0004697 | IDA:RGD | F | protein kinase C activity |
| GO:0008270 | IEA:InterPro | F | zinc ion binding |
| GO:0042113 | ISS:UniProtKB | P | B cell activation |
| GO:0050853 | ISS:UniProtKB | P | B cell receptor signaling pathway |
| GO:0006915 | IEA:UniProtKB-KW | P | apoptotic process |
| GO:0018894 | IEP:RGD | P | dibenzo-p-dioxin metabolic process |
| GO:0035408 | ISS:UniProtKB | P | histone H3-T6 phosphorylation |
| GO:0035556 | NAS:RGD | P | intracellular signal transduction |
| GO:0010829 | ISS:UniProtKB | P | negative regulation of glucose transport |
| GO:0046627 | IDA:UniProtKB | P | negative regulation of insulin receptor signaling pathway |
| GO:0050861 | ISS:UniProtKB | P | positive regulation of B cell receptor signaling pathway |
| GO:0043123 | ISS:UniProtKB | P | positive regulation of I-kappaB kinase/NF-kappaB signaling |
| GO:0051092 | ISS:UniProtKB | P | positive regulation of NF-kappaB transcription factor activity |
| GO:0045766 | ISS:UniProtKB | P | positive regulation of angiogenesis |
| GO:0042488 | IEP:RGD | P | positive regulation of odontogenesis of dentin-containing tooth |
| GO:0030949 | ISS:UniProtKB | P | positive regulation of vascular endothelial growth factor receptor signaling pathway |
| GO:0014059 | IMP:RGD | P | regulation of dopamine secretion |
| GO:0040008 | IMP:RGD | P | regulation of growth |
| GO:0006357 | ISS:UniProtKB | P | regulation of transcription from RNA polymerase II promoter |
| GO:0042493 | IMP:RGD | P | response to drug |
| GO:0045471 | IEP:RGD | P | response to ethanol |
| GO:0009749 | IEP:RGD | P | response to glucose |
| GO:0033280 | IEP:RGD | P | response to vitamin D |
| GO:0006351 | IEA:UniProtKB-KW | P | transcription, DNA-templated |
KEGG Pathway Links
| KEGG Pathway ID | Description |
|---|---|
| ko05143 | African trypanosomiasis |
| rno05143 | African trypanosomiasis |
| ko04960 | Aldosterone-regulated sodium reabsorption |
| rno04960 | Aldosterone-regulated sodium reabsorption |
| ko05146 | Amoebiasis |
| rno05146 | Amoebiasis |
| ko05031 | Amphetamine addiction |
| rno05031 | Amphetamine addiction |
| ko04662 | B cell receptor signaling pathway |
| rno04662 | B cell receptor signaling pathway |
| ko04020 | Calcium signaling pathway |
| rno04020 | Calcium signaling pathway |
| ko04973 | Carbohydrate digestion and absorption |
| rno04973 | Carbohydrate digestion and absorption |
| ko04062 | Chemokine signaling pathway |
| rno04062 | Chemokine signaling pathway |
| rno04725 | Cholinergic synapse |
| ko04713 | Circadian entrainment |
| rno04713 | Circadian entrainment |
| ko04728 | Dopaminergic synapse |
| rno04728 | Dopaminergic synapse |
| ko04961 | Endocrine and other factor-regulated calcium reabsorption |
| rno04961 | Endocrine and other factor-regulated calcium reabsorption |
| ko04012 | ErbB signaling pathway |
| rno04012 | ErbB signaling pathway |
| ko04666 | Fc gamma R-mediated phagocytosis |
| rno04666 | Fc gamma R-mediated phagocytosis |
| ko04510 | Focal adhesion |
| rno04510 | Focal adhesion |
| ko04727 | GABAergic synapse |
| rno04727 | GABAergic synapse |
| ko04540 | Gap junction |
| rno04540 | Gap junction |
| ko04971 | Gastric acid secretion |
| rno04971 | Gastric acid secretion |
| ko05214 | Glioma |
| rno05214 | Glioma |
| ko04724 | Glutamatergic synapse |
| rno04724 | Glutamatergic synapse |
| ko04912 | GnRH signaling pathway |
| rno04912 | GnRH signaling pathway |
| rno04066 | HIF-1 signaling pathway |
| rno05161 | Hepatitis B |
| ko04750 | Inflammatory mediator regulation of TRP channels |
| rno04750 | Inflammatory mediator regulation of TRP channels |
| ko05164 | Influenza A |
| rno05164 | Influenza A |
| rno04911 | Insulin secretion |
| ko05140 | Leishmaniasis |
| rno05140 | Leishmaniasis |
| ko04670 | Leukocyte transendothelial migration |
| rno04670 | Leukocyte transendothelial migration |
| ko04730 | Long-term depression |
| rno04730 | Long-term depression |
| ko04720 | Long-term potentiation |
| rno04720 | Long-term potentiation |
| ko04010 | MAPK signaling pathway |
| rno04010 | MAPK signaling pathway |
| ko04916 | Melanogenesis |
| rno04916 | Melanogenesis |
| ko05206 | MicroRNAs in cancer |
| rno05206 | MicroRNAs in cancer |
| ko05032 | Morphine addiction |
| rno05032 | Morphine addiction |
| rno04064 | NF-kappa B signaling pathway |
| ko04650 | Natural killer cell mediated cytotoxicity |
| rno04650 | Natural killer cell mediated cytotoxicity |
| ko05223 | Non-small cell lung cancer |
| rno05223 | Non-small cell lung cancer |
| ko04921 | Oxytocin signaling pathway |
| rno04921 | Oxytocin signaling pathway |
| ko04972 | Pancreatic secretion |
| rno04972 | Pancreatic secretion |
| rno05200 | Pathways in cancer |
| ko04070 | Phosphatidylinositol signaling system |
| rno04070 | Phosphatidylinositol signaling system |
| ko05205 | Proteoglycans in cancer |
| rno05205 | Proteoglycans in cancer |
| ko04015 | Rap1 signaling pathway |
| rno04015 | Rap1 signaling pathway |
| rno04014 | Ras signaling pathway |
| ko04723 | Retrograde endocannabinoid signaling |
| rno04723 | Retrograde endocannabinoid signaling |
| ko04970 | Salivary secretion |
| rno04970 | Salivary secretion |
| rno04726 | Serotonergic synapse |
| rno04919 | Thyroid hormone signaling pathway |
| ko04918 | Thyroid hormone synthesis |
| rno04918 | Thyroid hormone synthesis |
| ko04530 | Tight junction |
| rno04530 | Tight junction |
| ko04370 | VEGF signaling pathway |
| rno04370 | VEGF signaling pathway |
| ko04270 | Vascular smooth muscle contraction |
| rno04270 | Vascular smooth muscle contraction |
| ko04310 | Wnt signaling pathway |
| rno04310 | Wnt signaling pathway |
| ko04150 | mTOR signaling pathway |
| rno04150 | mTOR signaling pathway |
REACTOME Pathway Links
| REACTOME Pathway ID | Description |
|---|---|
| 5954408 | Activation of NF-kappaB in B cells |
| 5953415 | Adaptive Immune System |
| 5953205 | Cell Cycle |
| 5953204 | Cell Cycle, Mitotic |
| 5954635 | Depolymerisation of the Nuclear Lamina |
| 5953652 | Disinhibition of SNARE formation |
| 5953994 | Downstream signaling events of B Cell Receptor (BCR) |
| 5954206 | G alpha (z) signalling events |
| 5953642 | GPCR downstream signaling |
| 5954212 | Glutamate Binding, Activation of AMPA Receptors and Synaptic Plasticity |
| 5953645 | Hemostasis |
| 5953410 | Immune System |
| 5953688 | M Phase |
| 5954560 | Mitotic Prophase |
| 5953277 | Neuronal System |
| 5953606 | Neurotransmitter Receptor Binding And Downstream Transmission In The Postsynaptic Cell |
| 5954597 | Nuclear Envelope Breakdown |
| 5954628 | PCP/CE pathway |
| 5953644 | Platelet activation, signaling and aggregation |
| 5953646 | Response to elevated platelet cytosolic Ca2+ |
| 5953381 | Signal Transduction |
| 5953391 | Signaling by GPCR |
| 5953420 | Signaling by VEGF |
| 5953803 | Signaling by Wnt |
| 5953414 | Signaling by the B Cell Receptor (BCR) |
| 5954228 | Trafficking of AMPA receptors |
| 5954229 | Trafficking of GluR2-containing AMPA receptors |
| 5953276 | Transmission across Chemical Synapses |
| 5953419 | VEGFA-VEGFR2 Pathway |
| 5953418 | VEGFR2 mediated cell proliferation |
| 5954639 | WNT5A-dependent internalization of FZD4 |
| 5953802 | beta-catenin independent WNT signaling |
Domain Information
InterPro Annotations
| Accession | Description |
|---|---|
| IPR000961 | AGC-kinase, C-terminal |
| IPR000008 | C2 domain |
| IPR020454 | Diacylglycerol/phorbol-ester binding |
| IPR014375 | Protein kinase C, alpha/beta/gamma types |
| IPR002219 | Protein kinase C-like, phorbol ester/diacylglycerol-binding domain |
| IPR000719 | Protein kinase domain |
| IPR017441 | Protein kinase, ATP binding site |
| IPR017892 | Protein kinase, C-terminal |
| IPR011009 | Protein kinase-like domain |
| IPR008271 | Serine/threonine-protein kinase, active site |
| IPR002290 | Serine/threonine/dual specificity protein kinase, catalytic domain |
UniProt Annotations
Entry Information
Comments
| Comment Type | Description |
|---|---|
| Alternative Products | Event=Alternative splicing; Named isoforms=2; Name=Beta-I; IsoId=P68403-1, P04410-1; Sequence=Displayed; Name=Beta-II; IsoId=P68403-2, P04410-2; Sequence=VSP_004739; |
| Catalytic Activity | ATP + a protein = ADP + a phosphoprotein. |
| Cofactor | Name=Ca(2+); Xref=ChEBI:CHEBI:29108; Note=Binds 3 Ca(2+) ions per subunit. The ions are bound to the C2 domain.; |
| Enzyme Regulation | Classical (or conventional) PKCs (PRKCA, PRKCB and PRKCG) are activated by calcium and diacylglycerol (DAG) in the presence of phosphatidylserine. Three specific sites; Thr-500 (activation loop of the kinase domain), Thr-642 (turn motif) and Ser-661 (hydrophobic region), need to be phosphorylated for its full activation. Specifically inhibited by enzastaurin (LY317615) (By similarity) |
| Function | Calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase involved in various cellular processes such as regulation of the B-cell receptor (BCR) signalosome, oxidative stress-induced apoptosis, androgen receptor-dependent transcription regulation, insulin signaling and endothelial cells proliferation. Plays a key role in B-cell activation by regulating BCR-induced NF-kappa-B activation. Mediates the activation of the canonical NF-kappa-B pathway (NFKB1) by direct phosphorylation of CARD11/CARMA1 at 'Ser-559', 'Ser-644' and 'Ser-652'. Phosphorylation induces CARD11/CARMA1 association with lipid rafts and recruitment of the BCL10-MALT1 complex as well as MAP3K7/TAK1, which then activates IKK complex, resulting in nuclear translocation and activation of NFKB1. Plays a direct role in the negative feedback regulation of the BCR signaling, by down-modulating BTK function via direct phosphorylation of BTK at 'Ser-180', which results in the alteration of BTK plasma membrane localization and in turn inhibition of BTK activity. Involved in apoptosis following oxidative damage: in case of oxidative conditions, specifically phosphorylates 'Ser-36' of isoform p66Shc of SHC1, leading to mitochondrial accumulation of p66Shc, where p66Shc acts as a reactive oxygen species producer. Acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and specifically mediating phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag for epigenetic transcriptional activation that prevents demethylation of histone H3 'Lys-4' (H3K4me) by LSD1/KDM1A. In insulin signaling, may function downstream of IRS1 in muscle cells and mediate insulin-dependent DNA synthesis through the RAF1- MAPK/ERK signaling cascade. May participate in the regulation of glucose transport in adipocytes by negatively modulating the insulin-stimulated translocation of the glucose transporter SLC2A4/GLUT4. Under high glucose in pancreatic beta-cells, is probably involved in the inhibition of the insulin gene transcription, via regulation of MYC expression. In endothelial cells, activation of PRKCB induces increased phosphorylation of RB1, increased VEGFA-induced cell proliferation, and inhibits PI3K/AKT-dependent nitric oxide synthase (NOS3/eNOS) regulation by insulin, which causes endothelial dysfunction. Also involved in triglyceride homeostasis (By similarity). Phosphorylates ATF2 which promotes cooperation between ATF2 and JUN, activating transcription. {ECO:0000250, ECO:0000269|PubMed:10938109, ECO:0000269|PubMed:11714718, ECO:0000269|PubMed:19176525}. |
| Interaction | P18052:Ptpra (xeno); NbExp=3; IntAct=EBI-397072, EBI-6597520; Q8WV44-2:TRIM41 (xeno); NbExp=3; IntAct=EBI-397092, EBI-726015; |
| Ptm | Phosphorylation on Thr-500 of isoform beta-I, within the activation loop, renders it competent to autophosphorylate. Subsequent autophosphorylation of Thr-642 maintains catalytic competence, and autophosphorylation on Ser-661 appears to release the kinase into the cytosol. Similarly, isoform beta-II is autophosphorylated on 'Thr-641' and 'Ser-660', subsequent to phosphorylation on Thr-500. Autophosphorylated on other sites i.e. in the N-terminal and hinge regions have no effect on enzyme activity. Phosphorylation at Tyr-662 by SYK induces binding with GRB2 and contributes to the activation of MAPK/ERK signaling cascade. {ECO:0000269|PubMed:21215369, ECO:0000269|PubMed:7961692, ECO:0000269|PubMed:8327493, ECO:0000269|PubMed:8749392}. |
| Similarity | Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily |
| Similarity | Contains 1 AGC-kinase C-terminal domain |
| Similarity | Contains 1 C2 domain. {ECO:0000255|PROSITE- ProRule:PRU00041}. |
| Similarity | Contains 1 protein kinase domain |
| Similarity | Contains 2 phorbol-ester/DAG-type zinc fingers |
| Subcellular Location | Cytoplasm. Nucleus . Membrane; Peripheral membrane protein. |
| Subunit | Interacts with PDK1. Interacts in vitro with PRKCBP1. Interacts with PHLPP1 and PHLPP2; both proteins mediate its dephosphorylation. Interacts with KDM1A/LSD1, PKN1 and ANDR (By similarity) |
Identical and Related Proteins
Unique RefSeq proteins for LMP012456 (as displayed in Record Overview)
| Protein GI | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| 288806592 | RefSeq | NP_036845 | 673 | protein kinase C beta type isoform 1 |
| 288806594 | RefSeq | NP_001165776 | 671 | protein kinase C beta type isoform 2 |
Identical Sequences to LMP012456 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|
Related Sequences to LMP012456 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:288806592 | GenBank | AAI27084.1 | 673 | Protein kinase C, beta [Mus musculus] |
| GI:288806592 | GenBank | ABK42345.1 | 673 | protein kinase C beta2 [synthetic construct] |
| GI:288806592 | RefSeq | NP_032881.1 | 673 | protein kinase C beta type [Mus musculus] |
| GI:288806594 | RefSeq | XP_006507505.1 | 671 | PREDICTED: protein kinase C beta type isoform X1 [Mus musculus] |
| GI:288806594 | SwissProt | P68403.3 | 671 | RecName: Full=Protein kinase C beta type; Short=PKC-B; Short=PKC-beta [Rattus norvegicus] |
| GI:288806594 | SwissProt | P68404.4 | 671 | RecName: Full=Protein kinase C beta type; Short=PKC-B; Short=PKC-beta [Mus musculus] |