Gene/Proteome Database (LMPD)

LMPD ID
LMP012456
Gene ID
25023
Species
Rattus norvegicus (Rat)
Gene Name
protein kinase C, beta
Gene Symbol
Prkcb
Synonyms
Pkcb; Prkcb1;
Alternate Names
protein kinase C beta type; PKC-beta; protein kinase C beta I; protein kinase C beta II; protein kinase C, beta 1;
Chromosome
1
Map Location
1q36
EC Number
2.7.11.13;
Summary
plays a role in hexosamine biosynthesis pathway induced transcriptional regulation [RGD, Feb 2006]
Orthologs
View orthologs and multiple alignments for Prkcb

Proteins

protein kinase C beta type isoform 1
Refseq ID NP_036845
Protein GI 288806592
UniProt ID P68403
mRNA ID NM_012713
Length 673
MADPAAGPPPSEGEESTVRFARKGALRQKNVHEVKNHKFTARFFKQPTFCSHCTDFIWGLGLQGFQSQVCCFVVHKRCHEFVTFSCPGADKGPASDDPRSKHKFKIHTYSSPTFCDHCGSLLYGLIHQGMKCDTCMMNVHKRCVMNVPSLCGTDHTERRGRIYIQAHIDREVLIVVVRDAKNLVPMDPNGLSDPYVKLKLIPDPKSESKQKTKTIKCSLNPEWNETFRFQLKESDKDRRLSVEIWDWDLTSRNDFMGSLSFGISELQKAGVDGWFKLLSQEEGEYFNVPVPPEGSEGNEELRQKFERAKIGQGTKAPEEKTANTISKFDNNGNRDRMKLTDFNFLMVLGKGSFGKVMLSERKGTDELYAVKILKKDVVIQDDDVECTMVEKRVLALPGKPPFLTQLHSCFQTMDRLYFVMEYVNGGDLMYHIQQVGRFKEPHAVFYAAEIAIGLFFLQSKGIIYRDLKLDNVMLDSEGHIKIADFGMCKENIWDGVTTKTFCGTPDYIAPEIIAYQPYGKSVDWWAFGVLLYEMLAGQAPFEGEDEDELFQSIMEHNVAYPKSMSKEAVAICKGLMTKHPGKRLGCGPEGERDIKEHAFFRYIDWEKLERKEIQPPYKPKACGRNAENFDRFFTRHPPVLTPPDQEVIRNIDQSEFEGFSFVNSEFLKPEVKS
protein kinase C beta type isoform 2
Refseq ID NP_001165776
Protein GI 288806594
UniProt ID P68403
mRNA ID NM_001172305
Length 671
MADPAAGPPPSEGEESTVRFARKGALRQKNVHEVKNHKFTARFFKQPTFCSHCTDFIWGLGLQGFQSQVCCFVVHKRCHEFVTFSCPGADKGPASDDPRSKHKFKIHTYSSPTFCDHCGSLLYGLIHQGMKCDTCMMNVHKRCVMNVPSLCGTDHTERRGRIYIQAHIDREVLIVVVRDAKNLVPMDPNGLSDPYVKLKLIPDPKSESKQKTKTIKCSLNPEWNETFRFQLKESDKDRRLSVEIWDWDLTSRNDFMGSLSFGISELQKAGVDGWFKLLSQEEGEYFNVPVPPEGSEGNEELRQKFERAKIGQGTKAPEEKTANTISKFDNNGNRDRMKLTDFNFLMVLGKGSFGKVMLSERKGTDELYAVKILKKDVVIQDDDVECTMVEKRVLALPGKPPFLTQLHSCFQTMDRLYFVMEYVNGGDLMYHIQQVGRFKEPHAVFYAAEIAIGLFFLQSKGIIYRDLKLDNVMLDSEGHIKIADFGMCKENIWDGVTTKTFCGTPDYIAPEIIAYQPYGKSVDWWAFGVLLYEMLAGQAPFEGEDEDELFQSIMEHNVAYPKSMSKEAVAICKGLMTKHPGKRLGCGPEGERDIKEHAFFRYIDWEKLERKEIQPPYKPKARDKRDTSNFDKEFTRQPVELTPTDKLFIMNLDQNEFAGFSYTNPEFVINV

Gene Information

Entrez Gene ID
25023
Gene Name
protein kinase C, beta
Gene Symbol
Prkcb
Species
Rattus norvegicus

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0031526 IDA:RGD C brush border membrane
GO:0005813 IDA:RGD C centrosome
GO:0005829 IDA:RGD C cytosol
GO:0005634 ISS:UniProtKB C nucleus
GO:0005886 IDA:RGD C plasma membrane
GO:0005524 IEA:UniProtKB-KW F ATP binding
GO:0050681 ISS:UniProtKB F androgen receptor binding
GO:0003682 ISS:UniProtKB F chromatin binding
GO:0042393 ISS:UniProtKB F histone binding
GO:0035403 ISS:UniProtKB F histone kinase activity (H3-T6 specific)
GO:0030374 ISS:UniProtKB F ligand-dependent nuclear receptor transcription coactivator activity
GO:0004697 IDA:RGD F protein kinase C activity
GO:0008270 IEA:InterPro F zinc ion binding
GO:0042113 ISS:UniProtKB P B cell activation
GO:0050853 ISS:UniProtKB P B cell receptor signaling pathway
GO:0006915 IEA:UniProtKB-KW P apoptotic process
GO:0018894 IEP:RGD P dibenzo-p-dioxin metabolic process
GO:0035408 ISS:UniProtKB P histone H3-T6 phosphorylation
GO:0035556 NAS:RGD P intracellular signal transduction
GO:0010829 ISS:UniProtKB P negative regulation of glucose transport
GO:0046627 IDA:UniProtKB P negative regulation of insulin receptor signaling pathway
GO:0050861 ISS:UniProtKB P positive regulation of B cell receptor signaling pathway
GO:0043123 ISS:UniProtKB P positive regulation of I-kappaB kinase/NF-kappaB signaling
GO:0051092 ISS:UniProtKB P positive regulation of NF-kappaB transcription factor activity
GO:0045766 ISS:UniProtKB P positive regulation of angiogenesis
GO:0042488 IEP:RGD P positive regulation of odontogenesis of dentin-containing tooth
GO:0030949 ISS:UniProtKB P positive regulation of vascular endothelial growth factor receptor signaling pathway
GO:0014059 IMP:RGD P regulation of dopamine secretion
GO:0040008 IMP:RGD P regulation of growth
GO:0006357 ISS:UniProtKB P regulation of transcription from RNA polymerase II promoter
GO:0042493 IMP:RGD P response to drug
GO:0045471 IEP:RGD P response to ethanol
GO:0009749 IEP:RGD P response to glucose
GO:0033280 IEP:RGD P response to vitamin D
GO:0006351 IEA:UniProtKB-KW P transcription, DNA-templated

KEGG Pathway Links

KEGG Pathway ID Description
ko05143 African trypanosomiasis
rno05143 African trypanosomiasis
ko04960 Aldosterone-regulated sodium reabsorption
rno04960 Aldosterone-regulated sodium reabsorption
ko05146 Amoebiasis
rno05146 Amoebiasis
ko05031 Amphetamine addiction
rno05031 Amphetamine addiction
ko04662 B cell receptor signaling pathway
rno04662 B cell receptor signaling pathway
ko04020 Calcium signaling pathway
rno04020 Calcium signaling pathway
ko04973 Carbohydrate digestion and absorption
rno04973 Carbohydrate digestion and absorption
ko04062 Chemokine signaling pathway
rno04062 Chemokine signaling pathway
rno04725 Cholinergic synapse
ko04713 Circadian entrainment
rno04713 Circadian entrainment
ko04728 Dopaminergic synapse
rno04728 Dopaminergic synapse
ko04961 Endocrine and other factor-regulated calcium reabsorption
rno04961 Endocrine and other factor-regulated calcium reabsorption
ko04012 ErbB signaling pathway
rno04012 ErbB signaling pathway
ko04666 Fc gamma R-mediated phagocytosis
rno04666 Fc gamma R-mediated phagocytosis
ko04510 Focal adhesion
rno04510 Focal adhesion
ko04727 GABAergic synapse
rno04727 GABAergic synapse
ko04540 Gap junction
rno04540 Gap junction
ko04971 Gastric acid secretion
rno04971 Gastric acid secretion
ko05214 Glioma
rno05214 Glioma
ko04724 Glutamatergic synapse
rno04724 Glutamatergic synapse
ko04912 GnRH signaling pathway
rno04912 GnRH signaling pathway
rno04066 HIF-1 signaling pathway
rno05161 Hepatitis B
ko04750 Inflammatory mediator regulation of TRP channels
rno04750 Inflammatory mediator regulation of TRP channels
ko05164 Influenza A
rno05164 Influenza A
rno04911 Insulin secretion
ko05140 Leishmaniasis
rno05140 Leishmaniasis
ko04670 Leukocyte transendothelial migration
rno04670 Leukocyte transendothelial migration
ko04730 Long-term depression
rno04730 Long-term depression
ko04720 Long-term potentiation
rno04720 Long-term potentiation
ko04010 MAPK signaling pathway
rno04010 MAPK signaling pathway
ko04916 Melanogenesis
rno04916 Melanogenesis
ko05206 MicroRNAs in cancer
rno05206 MicroRNAs in cancer
ko05032 Morphine addiction
rno05032 Morphine addiction
rno04064 NF-kappa B signaling pathway
ko04650 Natural killer cell mediated cytotoxicity
rno04650 Natural killer cell mediated cytotoxicity
ko05223 Non-small cell lung cancer
rno05223 Non-small cell lung cancer
ko04921 Oxytocin signaling pathway
rno04921 Oxytocin signaling pathway
ko04972 Pancreatic secretion
rno04972 Pancreatic secretion
rno05200 Pathways in cancer
ko04070 Phosphatidylinositol signaling system
rno04070 Phosphatidylinositol signaling system
ko05205 Proteoglycans in cancer
rno05205 Proteoglycans in cancer
ko04015 Rap1 signaling pathway
rno04015 Rap1 signaling pathway
rno04014 Ras signaling pathway
ko04723 Retrograde endocannabinoid signaling
rno04723 Retrograde endocannabinoid signaling
ko04970 Salivary secretion
rno04970 Salivary secretion
rno04726 Serotonergic synapse
rno04919 Thyroid hormone signaling pathway
ko04918 Thyroid hormone synthesis
rno04918 Thyroid hormone synthesis
ko04530 Tight junction
rno04530 Tight junction
ko04370 VEGF signaling pathway
rno04370 VEGF signaling pathway
ko04270 Vascular smooth muscle contraction
rno04270 Vascular smooth muscle contraction
ko04310 Wnt signaling pathway
rno04310 Wnt signaling pathway
ko04150 mTOR signaling pathway
rno04150 mTOR signaling pathway

REACTOME Pathway Links

REACTOME Pathway ID Description
5954408 Activation of NF-kappaB in B cells
5953415 Adaptive Immune System
5953205 Cell Cycle
5953204 Cell Cycle, Mitotic
5954635 Depolymerisation of the Nuclear Lamina
5953652 Disinhibition of SNARE formation
5953994 Downstream signaling events of B Cell Receptor (BCR)
5954206 G alpha (z) signalling events
5953642 GPCR downstream signaling
5954212 Glutamate Binding, Activation of AMPA Receptors and Synaptic Plasticity
5953645 Hemostasis
5953410 Immune System
5953688 M Phase
5954560 Mitotic Prophase
5953277 Neuronal System
5953606 Neurotransmitter Receptor Binding And Downstream Transmission In The Postsynaptic Cell
5954597 Nuclear Envelope Breakdown
5954628 PCP/CE pathway
5953644 Platelet activation, signaling and aggregation
5953646 Response to elevated platelet cytosolic Ca2+
5953381 Signal Transduction
5953391 Signaling by GPCR
5953420 Signaling by VEGF
5953803 Signaling by Wnt
5953414 Signaling by the B Cell Receptor (BCR)
5954228 Trafficking of AMPA receptors
5954229 Trafficking of GluR2-containing AMPA receptors
5953276 Transmission across Chemical Synapses
5953419 VEGFA-VEGFR2 Pathway
5953418 VEGFR2 mediated cell proliferation
5954639 WNT5A-dependent internalization of FZD4
5953802 beta-catenin independent WNT signaling

Domain Information

InterPro Annotations

Accession Description
IPR000961 AGC-kinase, C-terminal
IPR000008 C2 domain
IPR020454 Diacylglycerol/phorbol-ester binding
IPR014375 Protein kinase C, alpha/beta/gamma types
IPR002219 Protein kinase C-like, phorbol ester/diacylglycerol-binding domain
IPR000719 Protein kinase domain
IPR017441 Protein kinase, ATP binding site
IPR017892 Protein kinase, C-terminal
IPR011009 Protein kinase-like domain
IPR008271 Serine/threonine-protein kinase, active site
IPR002290 Serine/threonine/dual specificity protein kinase, catalytic domain

UniProt Annotations

Entry Information

Gene Name
protein kinase C, beta
Protein Entry
KPCB_RAT
UniProt ID
P68403
Species
Rat

Comments

Comment Type Description
Alternative Products Event=Alternative splicing; Named isoforms=2; Name=Beta-I; IsoId=P68403-1, P04410-1; Sequence=Displayed; Name=Beta-II; IsoId=P68403-2, P04410-2; Sequence=VSP_004739;
Catalytic Activity ATP + a protein = ADP + a phosphoprotein.
Cofactor Name=Ca(2+); Xref=ChEBI:CHEBI:29108; Note=Binds 3 Ca(2+) ions per subunit. The ions are bound to the C2 domain.;
Enzyme Regulation Classical (or conventional) PKCs (PRKCA, PRKCB and PRKCG) are activated by calcium and diacylglycerol (DAG) in the presence of phosphatidylserine. Three specific sites; Thr-500 (activation loop of the kinase domain), Thr-642 (turn motif) and Ser-661 (hydrophobic region), need to be phosphorylated for its full activation. Specifically inhibited by enzastaurin (LY317615) (By similarity)
Function Calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase involved in various cellular processes such as regulation of the B-cell receptor (BCR) signalosome, oxidative stress-induced apoptosis, androgen receptor-dependent transcription regulation, insulin signaling and endothelial cells proliferation. Plays a key role in B-cell activation by regulating BCR-induced NF-kappa-B activation. Mediates the activation of the canonical NF-kappa-B pathway (NFKB1) by direct phosphorylation of CARD11/CARMA1 at 'Ser-559', 'Ser-644' and 'Ser-652'. Phosphorylation induces CARD11/CARMA1 association with lipid rafts and recruitment of the BCL10-MALT1 complex as well as MAP3K7/TAK1, which then activates IKK complex, resulting in nuclear translocation and activation of NFKB1. Plays a direct role in the negative feedback regulation of the BCR signaling, by down-modulating BTK function via direct phosphorylation of BTK at 'Ser-180', which results in the alteration of BTK plasma membrane localization and in turn inhibition of BTK activity. Involved in apoptosis following oxidative damage: in case of oxidative conditions, specifically phosphorylates 'Ser-36' of isoform p66Shc of SHC1, leading to mitochondrial accumulation of p66Shc, where p66Shc acts as a reactive oxygen species producer. Acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and specifically mediating phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag for epigenetic transcriptional activation that prevents demethylation of histone H3 'Lys-4' (H3K4me) by LSD1/KDM1A. In insulin signaling, may function downstream of IRS1 in muscle cells and mediate insulin-dependent DNA synthesis through the RAF1- MAPK/ERK signaling cascade. May participate in the regulation of glucose transport in adipocytes by negatively modulating the insulin-stimulated translocation of the glucose transporter SLC2A4/GLUT4. Under high glucose in pancreatic beta-cells, is probably involved in the inhibition of the insulin gene transcription, via regulation of MYC expression. In endothelial cells, activation of PRKCB induces increased phosphorylation of RB1, increased VEGFA-induced cell proliferation, and inhibits PI3K/AKT-dependent nitric oxide synthase (NOS3/eNOS) regulation by insulin, which causes endothelial dysfunction. Also involved in triglyceride homeostasis (By similarity). Phosphorylates ATF2 which promotes cooperation between ATF2 and JUN, activating transcription. {ECO:0000250, ECO:0000269|PubMed:10938109, ECO:0000269|PubMed:11714718, ECO:0000269|PubMed:19176525}.
Interaction P18052:Ptpra (xeno); NbExp=3; IntAct=EBI-397072, EBI-6597520; Q8WV44-2:TRIM41 (xeno); NbExp=3; IntAct=EBI-397092, EBI-726015;
Ptm Phosphorylation on Thr-500 of isoform beta-I, within the activation loop, renders it competent to autophosphorylate. Subsequent autophosphorylation of Thr-642 maintains catalytic competence, and autophosphorylation on Ser-661 appears to release the kinase into the cytosol. Similarly, isoform beta-II is autophosphorylated on 'Thr-641' and 'Ser-660', subsequent to phosphorylation on Thr-500. Autophosphorylated on other sites i.e. in the N-terminal and hinge regions have no effect on enzyme activity. Phosphorylation at Tyr-662 by SYK induces binding with GRB2 and contributes to the activation of MAPK/ERK signaling cascade. {ECO:0000269|PubMed:21215369, ECO:0000269|PubMed:7961692, ECO:0000269|PubMed:8327493, ECO:0000269|PubMed:8749392}.
Similarity Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily
Similarity Contains 1 AGC-kinase C-terminal domain
Similarity Contains 1 C2 domain. {ECO:0000255|PROSITE- ProRule:PRU00041}.
Similarity Contains 1 protein kinase domain
Similarity Contains 2 phorbol-ester/DAG-type zinc fingers
Subcellular Location Cytoplasm. Nucleus . Membrane; Peripheral membrane protein.
Subunit Interacts with PDK1. Interacts in vitro with PRKCBP1. Interacts with PHLPP1 and PHLPP2; both proteins mediate its dephosphorylation. Interacts with KDM1A/LSD1, PKN1 and ANDR (By similarity)

Identical and Related Proteins

Unique RefSeq proteins for LMP012456 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
288806592 RefSeq NP_036845 673 protein kinase C beta type isoform 1
288806594 RefSeq NP_001165776 671 protein kinase C beta type isoform 2

Identical Sequences to LMP012456 proteins

Reference Database Accession Length Protein Name

Related Sequences to LMP012456 proteins

Reference Database Accession Length Protein Name
GI:288806592 GenBank AAI27084.1 673 Protein kinase C, beta [Mus musculus]
GI:288806592 GenBank ABK42345.1 673 protein kinase C beta2 [synthetic construct]
GI:288806592 RefSeq NP_032881.1 673 protein kinase C beta type [Mus musculus]
GI:288806594 RefSeq XP_006507505.1 671 PREDICTED: protein kinase C beta type isoform X1 [Mus musculus]
GI:288806594 SwissProt P68403.3 671 RecName: Full=Protein kinase C beta type; Short=PKC-B; Short=PKC-beta [Rattus norvegicus]
GI:288806594 SwissProt P68404.4 671 RecName: Full=Protein kinase C beta type; Short=PKC-B; Short=PKC-beta [Mus musculus]