Gene/Proteome Database (LMPD)

LMPD ID
LMP012490
Gene ID
25279
Species
Rattus norvegicus (Rat)
Gene Name
cytochrome P450, family 24, subfamily a, polypeptide 1
Gene Symbol
Cyp24a1
Synonyms
Cyp24; VITD12; 25-hydroxyvitaminD324-hydroxylase;
Alternate Names
1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial; 24-OHase; 24R-Ohase; P450-CC24; cytochrome P450 24A1; cytochrome P450-CC24; vitamin D(3) 24-hydroxylase; cytochrome P450, subfamily 24; 25-hydroxyvitamin D3 24-hydroxylase; Cytochrom P450 (25-hydroxyvitamin D24-hydroxylase);
Chromosome
3
Map Location
3q42
EC Number
1.14.13.126
Summary
enzyme involved in vitamin D metabolism; linked to albuminuria, hyperlipidemia and atherosclerotic aorta lesions [RGD, Feb 2006]
Orthologs
View orthologs and multiple alignments for Cyp24a1

Proteins

1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial precursor
Refseq ID NP_963966
Protein GI 42761472
UniProt ID Q09128
mRNA ID NM_201635
Length 514
MSCPIDKRRTLIAFLRRLRDLGQPPRSVTSKASASRAPKEVPLCPLMTDGETRNVTSLPGPTNWPLLGSLLEIFWKGGLKKQHDTLAEYHKKYGQIFRMKLGSFDSVHLGSPSLLEALYRTESAHPQRLEIKPWKAYRDHRNEAYGLMILEGQEWQRVRSAFQKKLMKPVEIMKLDKKINEVLADFLERMDELCDERGRIPDLYSELNKWSFESICLVLYEKRFGLLQKETEEEALTFITAIKTMMSTFGKMMVTPVELHKRLNTKVWQAHTLAWDTIFKSVKPCIDNRLQRYSQQPGADFLCDIYQQDHLSKKELYAAVTELQLAAVETTANSLMWILYNLSRNPQAQRRLLQEVQSVLPDNQTPRAEDLRNMPYLKACLKESMRLTPSVPFTTRTLDKPTVLGEYALPKGTVLTLNTQVLGSSEDNFEDSHKFRPERWLQKEKKINPFAHLPFGIGKRMCIGRRLAELQLHLALCWIIQKYDIVATDNEPVEMLHLGILVPSRELPIAFRPR
transit_peptide: 1..35 experiment: experimental evidence, no additional details recorded note: Mitochondrion; propagated from UniProtKB/Swiss-Prot (Q09128.1) calculated_mol_wt: 3929 peptide sequence: MSCPIDKRRTLIAFLRRLRDLGQPPRSVTSKASAS mat_peptide: 36..514 product: 1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial experiment: experimental evidence, no additional details recorded note: propagated from UniProtKB/Swiss-Prot (Q09128.1) calculated_mol_wt: 55538 peptide sequence: RAPKEVPLCPLMTDGETRNVTSLPGPTNWPLLGSLLEIFWKGGLKKQHDTLAEYHKKYGQIFRMKLGSFDSVHLGSPSLLEALYRTESAHPQRLEIKPWKAYRDHRNEAYGLMILEGQEWQRVRSAFQKKLMKPVEIMKLDKKINEVLADFLERMDELCDERGRIPDLYSELNKWSFESICLVLYEKRFGLLQKETEEEALTFITAIKTMMSTFGKMMVTPVELHKRLNTKVWQAHTLAWDTIFKSVKPCIDNRLQRYSQQPGADFLCDIYQQDHLSKKELYAAVTELQLAAVETTANSLMWILYNLSRNPQAQRRLLQEVQSVLPDNQTPRAEDLRNMPYLKACLKESMRLTPSVPFTTRTLDKPTVLGEYALPKGTVLTLNTQVLGSSEDNFEDSHKFRPERWLQKEKKINPFAHLPFGIGKRMCIGRRLAELQLHLALCWIIQKYDIVATDNEPVEMLHLGILVPSRELPIAFRPR

Gene Information

Entrez Gene ID
25279
Gene Name
cytochrome P450, family 24, subfamily a, polypeptide 1
Gene Symbol
Cyp24a1
Species
Rattus norvegicus

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005739 TAS:RGD C mitochondrion
GO:0030342 TAS:BHF-UCL F 1-alpha,25-dihydroxyvitamin D3 24-hydroxylase activity
GO:0008403 IDA:RGD F 25-hydroxycholecalciferol-24-hydroxylase activity
GO:0020037 IEA:InterPro F heme binding
GO:0005506 IEA:InterPro F iron ion binding
GO:0001649 IEA:Ensembl P osteoblast differentiation
GO:0033280 IEA:Ensembl P response to vitamin D
GO:0042369 TAS:RGD P vitamin D catabolic process
GO:0042359 IDA:RGD P vitamin D metabolic process

KEGG Pathway Links

KEGG Pathway ID Description
rno01100 Metabolic pathways
ko05206 MicroRNAs in cancer
rno05206 MicroRNAs in cancer
ko00100 Steroid biosynthesis
rno00100 Steroid biosynthesis

REACTOME Pathway Links

REACTOME Pathway ID Description
5953258 Biological oxidations
5953485 Cytochrome P450 - arranged by substrate type
5953250 Metabolism
5953289 Metabolism of lipids and lipoproteins
5953937 Metabolism of steroid hormones and vitamin D
5953326 Phase 1 - Functionalization of compounds
5954056 Vitamin D (calciferol) metabolism
5954061 Vitamins

Domain Information

InterPro Annotations

Accession Description
IPR002949 Cyt_P450_E_CYP24A_mit
IPR001128 Cytochrome P450
IPR017972 Cytochrome P450, conserved site

UniProt Annotations

Entry Information

Gene Name
cytochrome P450, family 24, subfamily a, polypeptide 1
Protein Entry
CP24A_RAT
UniProt ID
Q09128
Species
Rat

Comments

Comment Type Description
Biophysicochemical Properties Kinetic parameters: KM=2.8 uM for calcidiol ; pH dependence: Optimum pH is 7.7. ;
Catalytic Activity Calcidiol + NADPH + O(2) = secalciferol + NADP(+) + H(2)O
Catalytic Activity Calcitriol + NADPH + O(2) = calcitetrol + NADP(+) + H(2)O
Cofactor Name=heme; Xref=ChEBI:CHEBI:30413; Evidence= ;
Function Has a role in maintaining calcium homeostasis. Catalyzes the NADPH-dependent 24-hydroxylation of calcidiol (25- hydroxyvitamin D(3)) and calcitriol (1-alpha,25-dihydroxyvitamin D(3)). The enzyme can perform up to 6 rounds of hydroxylation of calcitriol leading to calcitroic acid.
Similarity Belongs to the cytochrome P450 family
Subcellular Location Mitochondrion.

Identical and Related Proteins

Unique RefSeq proteins for LMP012490 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
42761472 RefSeq NP_963966 514 1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial precursor

Identical Sequences to LMP012490 proteins

Reference Database Accession Length Protein Name
GI:42761472 GenBank AAI00060.1 514 Cytochrome P450, subfamily 24 [Rattus norvegicus]
GI:42761472 GenBank EDL96333.1 514 cytochrome P450, subfamily 24 [Rattus norvegicus]
GI:42761472 RefSeq XP_006235734.1 514 PREDICTED: 1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial isoform X1 [Rattus norvegicus]
GI:42761472 SwissProt Q09128.1 514 RecName: Full=1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial; Short=24-OHase; Short=Vitamin D(3) 24-hydroxylase; AltName: Full=Cytochrome P450 24A1; AltName: Full=Cytochrome P450-CC24; Flags: Precursor [Rattus norvegicus]

Related Sequences to LMP012490 proteins

Reference Database Accession Length Protein Name
GI:42761472 EMBL CAA42093.1 514 25-hydroxyvitamin D3 24-hydroxylase [Rattus rattus]
GI:42761472 GenBank AAA42340.1 514 25-hydroxyvitamin D3 24-hydroxylase [Rattus norvegicus]
GI:42761472 SwissProt Q09128.1 514 RecName: Full=1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial; Short=24-OHase; Short=Vitamin D(3) 24-hydroxylase; AltName: Full=Cytochrome P450 24A1; AltName: Full=Cytochrome P450-CC24; Flags: Precursor [Rattus norvegicus]