Gene/Proteome Database (LMPD)

LMPD ID
LMP012494
Gene ID
25288
Species
Rattus norvegicus (Rat)
Gene Name
acyl-CoA synthetase long-chain family member 1
Gene Symbol
Acsl1
Synonyms
ACS; Acas; COAA; Facl2;
Alternate Names
long-chain-fatty-acid--CoA ligase 1; LACS 1; Acyl CoA synthetase, long chain; long-chain acyl-CoA synthetase 1; fatty acid Coenzyme A ligase, long chain 2; long-chain-fatty-acid--CoA ligase, liver isozyme;
Chromosome
16
Map Location
16q11
EC Number
6.2.1.3
Summary
catalyzes the ligation of long chain fatty acids with coenzyme A to produce long chain acyl-CoAs; plays a role in lipid biosynthesis and fatty acid metabolism [RGD, Feb 2006]
Orthologs
View orthologs and multiple alignments for Acsl1

Proteins

long-chain-fatty-acid--CoA ligase 1
Refseq ID NP_036952
Protein GI 25742739
UniProt ID P18163
mRNA ID NM_012820
Length 699
MEVHELFRYFRMPELIDIRQYVRTLPTNTLMGFGAFAALTTFWYATRPKALKPPCDLSMQSVEVTGTTEGVRRSAVLEDDKLLLYYYDDVRTMYDGFQRGIQVSNDGPCLGSRKPNQPYEWISYKQVAEMAECIGSALIQKGFKPCSEQFIGIFSQNRPEWVTIEQGCFTYSMVVVPLYDTLGTDAITYIVNKAELSVIFADKPEKAKLLLEGVENKLTPCLKIIVIMDSYDNDLVERGQKCGVEIIGLKALEDLGRVNRTKPKPPEPEDLAIICFTSGTTGNPKGAMVTHQNIMNDCSGFIKATESAFIASPEDVLISFLPLAHMFETVVECVMLCHGAKIGFFQGDIRLLMDDLKVLQPTIFPVVPRLLNRMFDRIFGQANTSVKRWLLDFASKRKEAELRSGIVRNNSLWDKLIFHKIQSSLGGKVRLMITGAAPVSATVLTFLRAALGCQFYEGYGQTECTAGCCLSLPGDWTAGHVGAPMPCNYIKLVDVEDMNYQAAKGEGEVCVKGANVFKGYLKDPARTAEALDKDGWLHTGDIGKWLPNGTLKIIDRKKHIFKLAQGEYIAPEKIENIYLRSEAVAQVFVHGESLQAFLIAIVVPDVEILPSWAQKRGFQGSFEELCRNKDINKAILEDMVKLGKNAGLKPFEQVKGIAVHPELFSIDNGLLTPTLKAKRPELRNYFRSQIDELYSTIKI

Gene Information

Entrez Gene ID
25288
Gene Name
acyl-CoA synthetase long-chain family member 1
Gene Symbol
Acsl1
Species
Rattus norvegicus

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005783 IDA:RGD C endoplasmic reticulum
GO:0016021 IEA:UniProtKB-KW C integral component of membrane
GO:0005741 IDA:RGD C mitochondrial outer membrane
GO:0005778 IDA:HGNC C peroxisomal membrane
GO:0005886 IDA:RGD C plasma membrane
GO:0003987 TAS:RGD F acetate-CoA ligase activity
GO:0005524 IEA:UniProtKB-KW F ATP binding
GO:0004467 IDA:UniProtKB F long-chain fatty acid-CoA ligase activity
GO:0033211 IEA:Ensembl P adiponectin-activated signaling pathway
GO:0006631 IMP:RGD P fatty acid metabolic process
GO:0015908 IGI:RGD P fatty acid transport
GO:0008610 IEA:Ensembl P lipid biosynthetic process
GO:0006629 TAS:RGD P lipid metabolic process
GO:0044539 IEA:Ensembl P long-chain fatty acid import
GO:0001676 IDA:GOC P long-chain fatty acid metabolic process
GO:0071902 IEA:Ensembl P positive regulation of protein serine/threonine kinase activity
GO:0042493 IEP:RGD P response to drug
GO:0007584 IEP:RGD P response to nutrient
GO:0034201 IEP:RGD P response to oleic acid
GO:0014070 IEP:RGD P response to organic cyclic compound
GO:0010033 IDA:RGD P response to organic substance
GO:0006641 IGI:RGD P triglyceride metabolic process
GO:0042178 IDA:RGD P xenobiotic catabolic process

KEGG Pathway Links

KEGG Pathway ID Description
ko04920 Adipocytokine signaling pathway
rno04920 Adipocytokine signaling pathway
M00086 beta-Oxidation, acyl-CoA synthesis
ko00061 Fatty acid biosynthesis
rno00061 Fatty acid biosynthesis
ko00071 Fatty acid degradation
rno00071 Fatty acid degradation
ko01212 Fatty acid metabolism
rno01212 Fatty acid metabolism
rno01100 Metabolic pathways
ko04146 Peroxisome
rno04146 Peroxisome
ko03320 PPAR signaling pathway
rno03320 PPAR signaling pathway

BIOCYC Pathway Links

BIOCYC Pathway ID Description
PWY66-387 fatty acid alpha-oxidation II

REACTOME Pathway Links

REACTOME Pathway ID Description
5954543 alpha-linolenic acid (ALA) metabolism
5954542 alpha-linolenic (omega3) and linoleic (omega6) acid metabolism
5953288 Fatty acid, triacylglycerol, and ketone body metabolism
5953463 Fatty Acyl-CoA Biosynthesis
5954541 Linoleic acid (LA) metabolism
5953250 Metabolism
5953289 Metabolism of lipids and lipoproteins
5954333 PPARA activates gene expression
5954222 Regulation of lipid metabolism by Peroxisome proliferator-activated receptor alpha (PPARalpha)
5953471 Synthesis of very long-chain fatty acyl-CoAs
5953464 Triglyceride Biosynthesis

Domain Information

InterPro Annotations

Accession Description
IPR020845 AMP-binding, conserved site
IPR000873 AMP-dependent synthetase/ligase

UniProt Annotations

Entry Information

Gene Name
acyl-CoA synthetase long-chain family member 1
Protein Entry
ACSL1_RAT
UniProt ID
P18163
Species
Rat

Comments

Comment Type Description
Biophysicochemical Properties Kinetic parameters: KM=649 uM for ATP ; KM=6.4 uM for CoA ; KM=5.0 uM for palmitate ; Vmax=1695 nmol/min/mg enzyme with palmitate as substrate ;
Catalytic Activity ATP + a long-chain fatty acid + CoA = AMP + diphosphate + an acyl-CoA.
Cofactor Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
Developmental Stage Levels remain constant during development.
Function Activation of long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. Preferentially uses oleate, arachidonate, eicosapentaenoate and docosahexaenoate as substrates.
Induction By high fat and high carbohydrate diets.
Miscellaneous 5 rat isozymes encoded by different genes have been described. ACSL6 corresponds to isozyme 2 (ACS2)
Similarity Belongs to the ATP-dependent AMP-binding enzyme family
Subcellular Location Mitochondrion outer membrane ; Single-pass type III membrane protein . Peroxisome membrane ; Single-pass type III membrane protein {ECO:0000250}. Microsome membrane ; Single-pass type III membrane protein . Endoplasmic reticulum membrane ; Single-pass type III membrane protein .
Tissue Specificity Liver, heart, epididymal adipose and to a lesser extent brain, small intestine and lung.

Identical and Related Proteins

Unique RefSeq proteins for LMP012494 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
25742739 RefSeq NP_036952 699 long-chain-fatty-acid--CoA ligase 1

Identical Sequences to LMP012494 proteins

Reference Database Accession Length Protein Name
GI:25742739 RefSeq XP_006253187.1 699 PREDICTED: long-chain-fatty-acid--CoA ligase 1 isoform X1 [Rattus norvegicus]
GI:25742739 RefSeq XP_006253188.1 699 PREDICTED: long-chain-fatty-acid--CoA ligase 1 isoform X1 [Rattus norvegicus]
GI:25742739 RefSeq XP_006253189.1 699 PREDICTED: long-chain-fatty-acid--CoA ligase 1 isoform X1 [Rattus norvegicus]
GI:25742739 RefSeq XP_008769476.1 699 PREDICTED: long-chain-fatty-acid--CoA ligase 1 isoform X1 [Rattus norvegicus]

Related Sequences to LMP012494 proteins

Reference Database Accession Length Protein Name
GI:25742739 DBBJ BAA14136.1 699 long-chain acyl-CoA synthetase [Rattus norvegicus]
GI:25742739 RefSeq XP_006253184.1 699 PREDICTED: long-chain-fatty-acid--CoA ligase 1 isoform X1 [Rattus norvegicus]
GI:25742739 SwissProt P18163.1 699 RecName: Full=Long-chain-fatty-acid--CoA ligase 1; AltName: Full=Long-chain acyl-CoA synthetase 1; Short=LACS 1; AltName: Full=Long-chain-fatty-acid--CoA ligase, liver isozyme [Rattus norvegicus]