Gene/Proteome Database (LMPD)

LMPD ID
LMP012495
Gene ID
25290
Species
Rattus norvegicus (Rat)
Gene Name
arachidonate 5-lipoxygenase
Gene Symbol
Alox5
Synonyms
LOX5A;
Alternate Names
arachidonate 5-lipoxygenase; 5-LO; 5-lipoxygenase; 5 - Lipoxygenase;
Chromosome
4
Map Location
4q42
EC Number
1.13.11.34;
Summary
catalyzes the conversion of arachidonate to leukotriene A4 in leukotriene metabolism [RGD, Feb 2006]
Orthologs
View orthologs and multiple alignments for Alox5

Proteins

arachidonate 5-lipoxygenase
Refseq ID NP_036954
Protein GI 6978493
UniProt ID P12527
mRNA ID NM_012822
Length 670
MPSYTVTVATGSQWFAGTDDYIYLSLIGSAGCSEKHLLDKAFYNDFERGGRDSYDVTVDEELGEIYLVKIEKRKYRLHDDWYLKYITLKTPHDYIEFPCYRWITGEGEIVLRDGCAKLARDDQIHILKQHRRKELETRQKQYRWMEWNPGFPLSIDAKCHKDLPRDIQFDSEKGVDFVLNYSKAMENLFINRFMHMFQSSWHDFADFEKIFVKISNTISERVKNHWQEDLMFGYQFLNGCNPVLIKRCTELPKKLPVTTEMVECSLERQLSLEQEVQEGNIFIVDYELLDGIDANKTDPCTHQFLAAPICLLYKNLANKIVPIAIQLNQTPGEKNPIFLPTDSKYDWLLAKIWVRSSDFHIHQTITHLLRTHLVSEVFGIAMYRQLPAVHPLFKLLVAHVRFTIAINTKAREQLNCEYGLFDKANATGGGGHVQMVQRAVQDLTYSSLCFPEAIKARGMDNTEDIPYYFYRDDGLLVWEAIQSFTTEVVSIYYEDDQVVEEDQELQDFVKDVYVYGMRGRKASGFPKSIKSREKLSEYLTVVIFTASAQHAAVNFGQYDWCSWIPNAPPTMRAPPPTAKGVVTIEQIVDTLPDRGRSCWHLGAVWALSQFQENELFLGMYPEEHFIEKPVKEAMIRFRKNLEAIVSVIAERNKNKKLPYYYLSPDRFQTV

Gene Information

Entrez Gene ID
25290
Gene Name
arachidonate 5-lipoxygenase
Gene Symbol
Alox5
Species
Rattus norvegicus

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005829 ISS:UniProtKB C cytosol
GO:0030425 IDA:RGD C dendrite
GO:0005635 IDA:RGD C nuclear envelope
GO:0005641 ISS:UniProtKB C nuclear envelope lumen
GO:0031965 ISS:UniProtKB C nuclear membrane
GO:0005634 IDA:RGD C nucleus
GO:0042383 IDA:RGD C sarcolemma
GO:0004051 IMP:RGD F arachidonate 5-lipoxygenase activity
GO:0005506 ISS:UniProtKB F iron ion binding
GO:0002526 IMP:RGD P acute inflammatory response
GO:0019369 IMP:RGD P arachidonic acid metabolic process
GO:0019370 ISS:UniProtKB P leukotriene biosynthetic process
GO:0019372 IMP:RGD P lipoxygenase pathway
GO:0045907 IMP:RGD P positive regulation of vasoconstriction
GO:0055093 IEP:RGD P response to hyperoxia
GO:0007584 IEP:RGD P response to nutrient
GO:0019233 IMP:RGD P sensory perception of pain

KEGG Pathway Links

KEGG Pathway ID Description
ko00590 Arachidonic acid metabolism
rno00590 Arachidonic acid metabolism
rno01100 Metabolic pathways
ko04913 Ovarian steroidogenesis
rno04913 Ovarian steroidogenesis
rno04726 Serotonergic synapse
ko05145 Toxoplasmosis
rno05145 Toxoplasmosis

REACTOME Pathway Links

REACTOME Pathway ID Description
5953493 Arachidonic acid metabolism
5953250 Metabolism
5953289 Metabolism of lipids and lipoproteins
5954095 Synthesis of 5-eicosatetraenoic acids
5954082 Synthesis of Leukotrienes (LT) and Eoxins (EX)
5954552 Synthesis of Lipoxins (LX)

Domain Information

InterPro Annotations

Accession Description
IPR008976 Lipase/lipooxygenase, PLAT/LH2
IPR000907 Lipoxygenase
IPR013819 Lipoxygenase, C-terminal
IPR020834 Lipoxygenase, conserved site
IPR020833 Lipoxygenase, iron binding site
IPR001885 Lipoxygenase, mammalian
IPR001024 PLAT/LH2 domain

UniProt Annotations

Entry Information

Gene Name
arachidonate 5-lipoxygenase
Protein Entry
LOX5_RAT
UniProt ID
P12527
Species
Rat

Comments

Comment Type Description
Catalytic Activity Arachidonate + O(2) = leukotriene A(4) + H(2)O.
Cofactor Name=Ca(2+); Xref=ChEBI:CHEBI:29108; Evidence= ; Note=Binds 2 calcium ions per subunit. ;
Cofactor Name=Fe cation; Xref=ChEBI:CHEBI:24875; Evidence= ; Note=Binds 1 Fe cation per subunit. {ECO:0000255|PROSITE- ProRule:PRU00726};
Function Catalyzes the first step in leukotriene biosynthesis, and thereby plays a role in inflammatory processes.
Pathway Lipid metabolism; leukotriene A4 biosynthesis.
Ptm Serine phosphorylation by MAPKAPK2 is stimulated by arachidonic acid. Phosphorylation on Ser-523 by PKA has an inhibitory effect. Phosphorylation on Ser-271 prevents export from the nucleus (By similarity)
Sequence Caution Sequence=AAA41538.1; Type=Frameshift; Positions=667; Evidence= ;
Similarity Belongs to the lipoxygenase family
Similarity Contains 1 PLAT domain. {ECO:0000255|PROSITE- ProRule:PRU00152}.
Similarity Contains 1 lipoxygenase domain. {ECO:0000255|PROSITE- ProRule:PRU00726}.
Subcellular Location Cytoplasm. Nucleus matrix. Nucleus membrane; Peripheral membrane protein. Note=Shuttles between cytoplasm and nucleus. Found exclusively in the nucleus, when phosphorylated on Ser-271. Calcium binding promotes translocation from the cytosol and the nuclear matrix to the nuclear envelope and membrane association.
Subunit Interacts with ALOX5AP and LTC4S. Interacts with COTL1, the interaction is required for stability and efficient catalytic activity (By similarity)

Identical and Related Proteins

Unique RefSeq proteins for LMP012495 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
6978493 RefSeq NP_036954 670 arachidonate 5-lipoxygenase

Identical Sequences to LMP012495 proteins

Reference Database Accession Length Protein Name
GI:6978493 GenBank AAA41538.1 670 5-lipoxygenase [Rattus norvegicus]
GI:6978493 GenBank AEU43499.1 670 Sequence 248 from patent US 8052970

Related Sequences to LMP012495 proteins

Reference Database Accession Length Protein Name
GI:6978493 GenBank AAA41538.1 670 5-lipoxygenase [Rattus norvegicus]
GI:6978493 GenBank AEU43499.1 670 Sequence 248 from patent US 8052970
GI:6978493 SwissProt P12527.3 673 RecName: Full=Arachidonate 5-lipoxygenase; Short=5-LO; Short=5-lipoxygenase [Rattus norvegicus]