Gene/Proteome Database (LMPD)

LMPD ID
LMP012510
Gene ID
25428
Species
Rattus norvegicus (Rat)
Gene Name
cytochrome P450, family 7, subfamily a, polypeptide 1
Gene Symbol
Cyp7a1
Synonyms
CHAP; CYP7; CYP7S1;
Alternate Names
cholesterol 7-alpha-monooxygenase; CYPVII; cytochrome P450, 7a1; cholesterol 7-alpha hydroxylase; cholesterol 7-alpha-hydroxylase; cytochrome P450 (cholesterol hydroxylase 7 alpha);
Chromosome
5
Map Location
5q12
EC Number
1.14.13.17
Summary
catalyzes the conversion of cholesterol, NADPH, and oxygen to 7-alpha-hydroxycholesterol, NADP(+) and water; plays a role in bile acid biosynthesis and cholesterol metabolism [RGD, Feb 2006]
Orthologs
View orthologs and multiple alignments for Cyp7a1

Proteins

cholesterol 7-alpha-monooxygenase
Refseq ID NP_037074
Protein GI 7549754
UniProt ID P18125
mRNA ID NM_012942
Length 503
MMTISLIWGIAVLVSCCIWFIVGIRRRKAGEPPLENGLIPYLGCALKFGSNPLEFLRANQRKHGHVFTCKLMGKYVHFITNSLSYHKVLCHGKYFDWKKFHYTTSAKAFGHRSIDPNDGNTTENINNTFTKTLQGDALCSLSEAMMQNLQSVMRPPGLPKSKSNAWVTEGMYAFCYRVMFEAGYLTLFGRDISKTDTQKALILNNLDNFKQFDQVFPALVAGLPIHLFKTAHKAREKLAEGLKHKNLCVRDQVSELIRLRMFLNDTLSTFDDMEKAKTHLAILWASQANTIPATFWSLFQMIRSPEAMKAASEEVSGALQSAGQELSSGGSAIYLDQVQLNDLPVLDSIIKEALRLSSASLNIRTAKEDFTLHLEDGSYNIRKDDMIALYPQLMHLDPEIYPDPLTFKYDRYLDESGKAKTTFYSNGNKLKCFYMPFGSGATICPGRLFAVQEIKQFLILMLSCFELEFVESQVKCPPLDQSRAGLGILPPLHDIEFKYKLKH

Gene Information

Entrez Gene ID
25428
Gene Name
cytochrome P450, family 7, subfamily a, polypeptide 1
Gene Symbol
Cyp7a1
Species
Rattus norvegicus

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005783 IEA:UniProtKB-KW C endoplasmic reticulum
GO:0043231 IDA:UniProtKB C intracellular membrane-bounded organelle
GO:0016020 IEA:UniProtKB-KW C membrane
GO:0008123 IDA:UniProtKB F cholesterol 7-alpha-monooxygenase activity
GO:0020037 IEA:InterPro F heme binding
GO:0005506 IEA:InterPro F iron ion binding
GO:0006699 IDA:UniProtKB P bile acid biosynthetic process
GO:0071397 IDA:UniProtKB P cellular response to cholesterol
GO:0071333 ISS:UniProtKB P cellular response to glucose stimulus
GO:0006707 IDA:UniProtKB P cholesterol catabolic process
GO:0042632 IDA:UniProtKB P cholesterol homeostasis
GO:0070857 IDA:UniProtKB P regulation of bile acid biosynthetic process

KEGG Pathway Links

KEGG Pathway ID Description
ko04976 Bile secretion
rno04976 Bile secretion
rno01100 Metabolic pathways
ko03320 PPAR signaling pathway
rno03320 PPAR signaling pathway
ko00120 Primary bile acid biosynthesis
rno00120 Primary bile acid biosynthesis
ko00140 Steroid hormone biosynthesis
rno00140 Steroid hormone biosynthesis

BIOCYC Pathway Links

BIOCYC Pathway ID Description
PWY-6061 bile acid biosynthesis, neutral pathway

REACTOME Pathway Links

REACTOME Pathway ID Description
5953720 Bile acid and bile salt metabolism
5953258 Biological oxidations
5953485 Cytochrome P450 - arranged by substrate type
5953932 Endogenous sterols
5953288 Fatty acid, triacylglycerol, and ketone body metabolism
5953250 Metabolism
5953289 Metabolism of lipids and lipoproteins
5954333 PPARA activates gene expression
5953326 Phase 1 - Functionalization of compounds
5954222 Regulation of lipid metabolism by Peroxisome proliferator-activated receptor alpha (PPARalpha)
5953930 Synthesis of bile acids and bile salts
5953931 Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol

Domain Information

InterPro Annotations

Accession Description
IPR001128 Cytochrome P450
IPR002403 Cytochrome P450, E-class, group IV
IPR024204 Cytochrome P450, cholesterol 7-alpha-monooxygenase-type
IPR017972 Cytochrome P450, conserved site

UniProt Annotations

Entry Information

Gene Name
cytochrome P450, family 7, subfamily a, polypeptide 1
Protein Entry
CP7A1_RAT
UniProt ID
P18125
Species
Rat

Comments

Comment Type Description
Catalytic Activity Cholesterol + NADPH + O(2) = 7-alpha- hydroxycholesterol + NADP(+) + H(2)O. {ECO:0000269|PubMed:1694852, ECO:0000269|PubMed:2335522}.
Cofactor Name=heme; Xref=ChEBI:CHEBI:30413; Evidence= ;
Enzyme Regulation Inhibition of activity by bile acid feedback.
Function Catalyzes a rate-limiting step in cholesterol catabolism and bile acid biosynthesis by introducing a hydrophilic moiety at position 7 of cholesterol. Important for cholesterol homeostasis
Induction Up-regulated by dietary cholesterol and cholestyramine. Down-regulated by dietary bile acids, such as chenodeoxycholic acid and cholic acid. {ECO:0000269|PubMed:11278771, ECO:0000269|PubMed:1694852, ECO:0000269|PubMed:2335522}.
Pathway Lipid metabolism; bile acid biosynthesis.
Similarity Belongs to the cytochrome P450 family
Subcellular Location Endoplasmic reticulum membrane ; Peripheral membrane protein . Microsome membrane ; Peripheral membrane protein .
Tissue Specificity Detected in liver (at protein level). Liver

Identical and Related Proteins

Unique RefSeq proteins for LMP012510 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
7549754 RefSeq NP_037074 503 cholesterol 7-alpha-monooxygenase

Identical Sequences to LMP012510 proteins

Reference Database Accession Length Protein Name
GI:7549754 GenBank AAB16290.1 503 Sequence 29 from patent US 5558999
GI:7549754 GenBank AAR81286.1 503 Sequence 2 from patent US 6660317
GI:7549754 GenBank EDM11635.1 503 cytochrome P450, family 7, subfamily a, polypeptide 1 [Rattus norvegicus]
GI:7549754 pat US 503 Sequence 29 from patent US 5753431

Related Sequences to LMP012510 proteins

Reference Database Accession Length Protein Name
GI:7549754 GenBank AAA40839.1 503 cholesterol 7-alpha-hydroxylase (EC 1.14.13.17) [Rattus norvegicus]
GI:7549754 GenBank AAA03649.1 503 cholesterol 7-alpha-hydroxylase [Rattus norvegicus]
GI:7549754 SwissProt P18125.1 503 RecName: Full=Cholesterol 7-alpha-monooxygenase; AltName: Full=CYPVII; AltName: Full=Cholesterol 7-alpha-hydroxylase; AltName: Full=Cytochrome P450 7A1 [Rattus norvegicus]