Gene/Proteome Database (LMPD)

LMPD ID
LMP012555
Gene ID
Species
Rattus norvegicus (Rat)
Gene Name
hepatocyte nuclear factor 4, alpha
Gene Symbol
Synonyms
Hnf4; Hnf4alpha;
Alternate Names
hepatocyte nuclear factor 4-alpha; TCF-14; HNF-4-alpha; transcription factor 14; transcription factor HNF-4; alpha transcription factor 4; hepatic nuclear factor 4, alpha; nuclear receptor subfamily 2 group A member 1;
Chromosome
3
Map Location
3q42-q43
Summary
steriod horomone receptor; involved in promoting transcription [RGD, Feb 2006]
Orthologs

Proteins

hepatocyte nuclear factor 4-alpha isoform 1
Refseq ID NP_071516
Protein GI 400153986
UniProt ID P22449
mRNA ID NM_022180
Length 474
MRLSKTLADMDMADYSAALDPAYTTLEFENVQVLTMGNDTSPSEGANLNSSNSLGVSALCAICGDRATGKHYGASSCDGCKGFFRRSVRKNHMYSCRFSRQCVVDKDKRNQCRYCRLKKCFRAGMKKEAVQNERDRISTRRSSYEDSSLPSINALLQAEVLSQQITSPISGINGDIRAKRIANITDVCESMKEQLLVLVEWAKYIPAFCELLLDDQVALLRAHAGEHLLLGATKRSMVFKDVLLLGNDYIVPRHCPELAEMSRVSIRILDELVLPFQELQIDDNEYACLKAIIFFDPDAKGLSDPGKIKRLRSQVQVSLEDYINDRQYDSRGRFGELLLLLPTLQSITWQMIEQIQFIKLFGMAKIDNLLQEMLLGGSASDAPHAHHPLHPHLMQEHMGTNVIVANTMPSHLSNGQMCEWPRPRGQAATPETPQPSPPSGSGSESYKLLPGAITTIVKPPSAIPQPTITKQEAI
hepatocyte nuclear factor 4-alpha isoform 2
Refseq ID NP_001257860
Protein GI 400153996
UniProt ID B9VVT3
mRNA ID NM_001270931
Length 449
MSDRGQGFLQDPPDTSPSEGANLNSSNSLGVSALCAICGDRATGKHYGASSCDGCKGFFRRSVRKNHMYSCRFSRQCVVDKDKRNQCRYCRLKKCFRAGMKKEAVQNERDRISTRRSSYEDSSLPSINALLQAEVLSQQITSPISGINGDIRAKRIANITDVCESMKEQLLVLVEWAKYIPAFCELLLDDQVALLRAHAGEHLLLGATKRSMVFKDVLLLGNDYIVPRHCPELAEMSRVSIRILDELVLPFQELQIDDNEYACLKAIIFFDPDAKGLSDPGKIKRLRSQVQVSLEDYINDRQYDSRGRFGELLLLLPTLQSITWQMIEQIQFIKLFGMAKIDNLLQEMLLGGSASDAPHAHHPLHPHLMQEHMGTNVIVANTMPSHLSNGQMCEWPRPRGQAATPETPQPSPPSGSGSESYKLLPGAITTIVKPPSAIPQPTITKQEAI
hepatocyte nuclear factor 4-alpha isoform 3
Refseq ID NP_001257862
Protein GI 400154015
UniProt ID B9VVT2
mRNA ID NM_001270933
Length 439
MSDRGQGFLQDPPDTSPSEGANLNSSNSLGVSALCAICGDRATGKHYGASSCDGCKGFFRRSVRKNHMYSCRFSRQCVVDKDKRNQCRYCRLKKCFRAGMKKEAVQNERDRISTRRSSYEDSSLPSINALLQAEVLSQQITSPISGINGDIRAKRIANITDVCESMKEQLLVLVEWAKYIPAFCELLLDDQVALLRAHAGEHLLLGATKRSMVFKDVLLLGNDYIVPRHCPELAEMSRVSIRILDELVLPFQELQIDDNEYACLKAIIFFDPDAKGLSDPGKIKRLRSQVQVSLEDYINDRQYDSRGRFGELLLLLPTLQSITWQMIEQIQFIKLFGMAKIDNLLQEMLLGGSASDAPHAHHPLHPHLMQEHMGTNVIVANTMPSHLSNGQMSTPETPQPSPPSGSGSESYKLLPGAITTIVKPPSAIPQPTITKQEAI

Gene Information

Entrez Gene ID
Gene Name
hepatocyte nuclear factor 4, alpha
Gene Symbol
Species
Rattus norvegicus

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005737 IDA:RGD C cytoplasm
GO:0005634 IDA:BHF-UCL C nucleus
GO:0005667 IDA:RGD C transcription factor complex
GO:0003677 IDA:RGD F DNA binding
GO:0001077 IDA:BHF-UCL F RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription
GO:0005504 IPI:RGD F fatty acid binding
GO:0000062 IPI:RGD F fatty-acyl-CoA binding
GO:0004879 IEA:InterPro F ligand-activated sequence-specific DNA binding RNA polymerase II transcription factor activity
GO:0016290 IDA:RGD F palmitoyl-CoA hydrolase activity
GO:0042803 IMP:RGD F protein homodimerization activity
GO:0070491 IPI:BHF-UCL F repressing transcription factor binding
GO:0043565 IMP:RGD F sequence-specific DNA binding
GO:0003700 IMP:RGD F sequence-specific DNA binding transcription factor activity
GO:0003707 TAS:RGD F steroid hormone receptor activity
GO:0008134 IPI:RGD F transcription factor binding
GO:0008270 IEA:InterPro F zinc ion binding
GO:0006637 IDA:RGD P acyl-CoA metabolic process
GO:0030154 IMP:RGD P cell differentiation
GO:0045216 IDA:RGD P cell-cell junction organization
GO:0007164 IDA:RGD P establishment of tissue polarity
GO:0008285 IDA:RGD P negative regulation of cell proliferation
GO:0045930 IDA:RGD P negative regulation of mitotic cell cycle
GO:0050732 IDA:RGD P negative regulation of peptidyl-tyrosine phosphorylation
GO:0033159 IDA:RGD P negative regulation of protein import into nucleus, translocation
GO:0043433 IDA:RGD P negative regulation of sequence-specific DNA binding transcription factor activity
GO:0042524 IDA:RGD P negative regulation of tyrosine phosphorylation of Stat5 protein
GO:0045723 IMP:RGD P positive regulation of fatty acid biosynthetic process
GO:0045722 IMP:RGD P positive regulation of gluconeogenesis
GO:0045944 IDA:BHF-UCL P positive regulation of transcription from RNA polymerase II promoter
GO:0045893 IDA:BHF-UCL P positive regulation of transcription, DNA-templated
GO:0032534 IMP:RGD P regulation of microvillus assembly
GO:0006355 IDA:RGD P regulation of transcription, DNA-templated
GO:0051591 IEP:RGD P response to cAMP
GO:0042493 IEP:RGD P response to drug
GO:0009749 IDA:RGD P response to glucose

KEGG Pathway Links

KEGG Pathway ID Description
ko04152 AMPK signaling pathway
rno04152 AMPK signaling pathway
ko04950 Maturity onset diabetes of the young
rno04950 Maturity onset diabetes of the young

REACTOME Pathway Links

REACTOME Pathway ID Description
5953533 Developmental Biology
5953330 Gene Expression
5953833 Generic Transcription Pathway
5954147 Nuclear Receptor transcription pathway
5954071 Regulation of beta-cell development
5954075 Regulation of gene expression in beta cells

Domain Information

InterPro Annotations

Accession Description
IPR008946 Nuclear hormone receptor, ligand-binding
IPR000536 Nuclear hormone receptor, ligand-binding, core
IPR001723 Steroid hormone receptor
IPR003068 Transcription factor COUP
IPR013088 Zinc finger, NHR/GATA-type
IPR001628 Zinc finger, nuclear hormone receptor-type

UniProt Annotations

Entry Information

Gene Name
hepatocyte nuclear factor 4, alpha
Protein Entry
HNF4A_RAT
UniProt ID
Species
Rat

Comments

Comment Type Description
Alternative Products Event=Alternative splicing; Named isoforms=2; Name=Long; IsoId=P22449-1; Sequence=Displayed; Name=Short; IsoId=P22449-2; Sequence=VSP_003677;
Function Transcriptionally controlled transcription factor. Binds to DNA sites required for the transcription of alpha 1- antitrypsin, apolipoprotein CIII, transthyretin genes and HNF1- alpha. May be essential for development of the liver, kidney and intestine.
Interaction P02692:Fabp1; NbExp=3; IntAct=EBI-5261592, EBI-1209448;
Miscellaneous Binds fatty acids.
Miscellaneous DNA-binding activity of phosphorylated protein is reduced by fasting and by inducers of intracellular cyclic AMP.
Ptm Acetylation at Lys-458 lowers transcriptional activation by about two-fold
Ptm Phosphorylation at Ser-313 by AMPK reduces the ability to form homodimers and bind DNA (By similarity). Phosphorylated in the recognition sequence R-R-S-S near the DNA-binding domain; phosphorylation results in decrease in DNA-binding activity. Phosphorylation of HNF4 depends on the diet and is decreased by a carbohydrate-rich diet and is increased by fasting. {ECO:0000250, ECO:0000269|PubMed:9234678}.
Ptm The N-terminus is blocked.
Sequence Caution Sequence=BAA01411.1; Type=Erroneous initiation; Note=Translation N-terminally extended.; Evidence= ; Sequence=CAA40412.1; Type=Erroneous initiation; Note=Translation N-terminally extended.; Evidence= ;
Similarity Belongs to the nuclear hormone receptor family. NR2 subfamily
Similarity Contains 1 nuclear receptor DNA-binding domain
Subcellular Location Nucleus.
Subunit Homodimerization is required for HNF4-alpha to bind to its recognition site. Interacts with PER2.
Tissue Specificity Liver, kidney and intestine.

Identical and Related Proteins

Unique RefSeq proteins for LMP012555 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
400153986 RefSeq NP_071516 474 hepatocyte nuclear factor 4-alpha isoform 1
400153996 RefSeq NP_001257860 449 hepatocyte nuclear factor 4-alpha isoform 2
400154015 RefSeq NP_001257862 439 hepatocyte nuclear factor 4-alpha isoform 3

Identical Sequences to LMP012555 proteins

Reference Database Accession Length Protein Name
GI:400153986 GenBank EDL96572.1 474 hepatocyte nuclear factor 4, alpha, isoform CRA_a [Rattus norvegicus]

Related Sequences to LMP012555 proteins

Reference Database Accession Length Protein Name
GI:400153986 GenBank AAX90602.1 474 hepatic nuclear factor 4 alpha [Mus musculus]
GI:400153986 GenBank EDL96572.1 474 hepatocyte nuclear factor 4, alpha, isoform CRA_a [Rattus norvegicus]
GI:400153996 GenBank EDL96573.1 452 hepatocyte nuclear factor 4, alpha, isoform CRA_b [Rattus norvegicus]
GI:400154015 RefSeq NP_001257860.1 449 hepatocyte nuclear factor 4-alpha isoform 2 [Rattus norvegicus]
GI:400153996 RefSeq XP_005363018.1 449 PREDICTED: hepatocyte nuclear factor 4-alpha isoform X3 [Microtus ochrogaster]
GI:400154015 RefSeq XP_005363019.1 439 PREDICTED: hepatocyte nuclear factor 4-alpha isoform X4 [Microtus ochrogaster]
GI:400153996 RefSeq XP_006971088.1 449 PREDICTED: hepatocyte nuclear factor 4-alpha isoform X3 [Peromyscus maniculatus bairdii]
GI:400154015 RefSeq XP_006971089.1 439 PREDICTED: hepatocyte nuclear factor 4-alpha isoform X4 [Peromyscus maniculatus bairdii]
GI:400153986 SwissProt P22449.3 474 RecName: Full=Hepatocyte nuclear factor 4-alpha; Short=HNF-4-alpha; AltName: Full=Nuclear receptor subfamily 2 group A member 1; AltName: Full=Transcription factor 14; Short=TCF-14; AltName: Full=Transcription factor HNF-4 [Rattus norvegicus]