Gene/Proteome Database (LMPD)

LMPD ID
LMP012598
Gene ID
29499
Species
Rattus norvegicus (Rat)
Gene Name
sonic hedgehog
Gene Symbol
Shh
Alternate Names
sonic hedgehog protein; sonic hedgehog homolog;
Chromosome
4
Map Location
4q11
Summary
plays a role in neurogenesis; regulates proliferation of adult hippocampal neural stem cells [RGD, Feb 2006]
Orthologs
View orthologs and multiple alignments for Shh

Proteins

sonic hedgehog protein precursor
Refseq ID NP_058917
Protein GI 8394267
UniProt ID Q63673
mRNA ID NM_017221
Length 437
MLLLLARCFLVALASSLLVCPGLACGPGRGFGKRQHPKKLTPLAYKQFIPNVAEKTLGASGRYEGKITRNSERFKELTPNYNPDIIFKDEENTGADRLMTQRCKDKLNALAISVMNQWPGVKLRVTEGWDEDGHHSEESLHYEGRAVDITTSDRDRSKYGMLARLAVEAGFDWVYYESKARIHCSVKAENSVAAKSDGCFPGSATVHLEQGGTKLVKDLSPGDRVLAADDQGRLLYSDFLTFLDRDEGAKKVFYVIETREPRERLLLTAAHLLFVAPHNDSGPTPGPSPLFASRVRPGQRVYVVAERGGDRRLLPAAVHSVTLREEAAGAYAPLTADGTILINRVLASCYAVIEEHSWAHRAFAPFRLAHALLAALAPARTDGGGGGSIPAPQSVAEARGAGPPAGIHWYSQLLYHIGTWLLDSETLHPLGMAVKSS
sig_peptide: 1..21 calculated_mol_wt: 2247 peptide sequence: MLLLLARCFLVALASSLLVCP mat_peptide: 25..437 product: Sonic hedgehog protein experiment: experimental evidence, no additional details recorded note: propagated from UniProtKB/Swiss-Prot (Q63673.1) calculated_mol_wt: 45160 peptide sequence: CGPGRGFGKRQHPKKLTPLAYKQFIPNVAEKTLGASGRYEGKITRNSERFKELTPNYNPDIIFKDEENTGADRLMTQRCKDKLNALAISVMNQWPGVKLRVTEGWDEDGHHSEESLHYEGRAVDITTSDRDRSKYGMLARLAVEAGFDWVYYESKARIHCSVKAENSVAAKSDGCFPGSATVHLEQGGTKLVKDLSPGDRVLAADDQGRLLYSDFLTFLDRDEGAKKVFYVIETREPRERLLLTAAHLLFVAPHNDSGPTPGPSPLFASRVRPGQRVYVVAERGGDRRLLPAAVHSVTLREEAAGAYAPLTADGTILINRVLASCYAVIEEHSWAHRAFAPFRLAHALLAALAPARTDGGGGGSIPAPQSVAEARGAGPPAGIHWYSQLLYHIGTWLLDSETLHPLGMAVKSS

Gene Information

Entrez Gene ID
29499
Gene Name
sonic hedgehog
Gene Symbol
Shh
Species
Rattus norvegicus

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0030424 IDA:RGD C axon
GO:0009986 ISS:UniProtKB C cell surface
GO:0030425 IDA:RGD C dendrite
GO:0005783 IDA:RGD C endoplasmic reticulum
GO:0005615 ISS:UniProtKB C extracellular space
GO:0005794 IDA:RGD C Golgi apparatus
GO:0045121 ISS:UniProtKB C membrane raft
GO:0043025 IDA:RGD C neuronal cell body
GO:0005886 IEA:UniProtKB-KW C plasma membrane
GO:0030133 IDA:RGD C transport vesicle
GO:0005509 ISS:UniProtKB F calcium ion binding
GO:0043237 ISS:UniProtKB F laminin-1 binding
GO:0008233 IEA:UniProtKB-KW F peptidase activity
GO:0005102 TAS:RGD F receptor binding
GO:0008270 ISS:UniProtKB F zinc ion binding
GO:0008209 ISS:UniProtKB P androgen metabolic process
GO:0097190 ISS:UniProt P apoptotic signaling pathway
GO:0007411 ISS:UniProtKB P axon guidance
GO:0001658 ISS:UniProtKB P branching involved in ureteric bud morphogenesis
GO:0048754 ISS:UniProtKB P branching morphogenesis of an epithelial tube
GO:0043369 ISS:UniProtKB P CD4-positive or CD8-positive, alpha-beta T cell lineage commitment
GO:0007267 ISS:UniProtKB P cell-cell signaling
GO:0048468 ISS:UniProtKB P cell development
GO:0001708 ISS:UniProtKB P cell fate specification
GO:0007417 ISS:UniProtKB P central nervous system development
GO:0021930 ISS:UniProtKB P cerebellar granule cell precursor proliferation
GO:0007502 IDA:RGD P digestive tract mesoderm development
GO:0009953 ISS:UniProtKB P dorsal/ventral pattern formation
GO:0009790 ISS:UniProtKB P embryo development
GO:0042733 ISS:UniProtKB P embryonic digit morphogenesis
GO:0030326 ISS:UniProtKB P embryonic limb morphogenesis
GO:0060441 IEP:RGD P epithelial tube branching involved in lung morphogenesis
GO:0030900 ISS:UniProtKB P forebrain development
GO:0061196 IMP:RGD P fungiform papilla development
GO:0061198 IMP:RGD P fungiform papilla formation
GO:0061197 IMP:RGD P fungiform papilla morphogenesis
GO:0007507 ISS:UniProtKB P heart development
GO:0030902 ISS:UniProtKB P hindbrain development
GO:0016539 IEA:InterPro P intein-mediated protein splicing
GO:0030324 ISS:UniProtKB P lung development
GO:0002320 ISS:UniProtKB P lymphoid progenitor cell differentiation
GO:0030539 ISS:UniProtKB P male genitalia development
GO:0048808 IMP:RGD P male genitalia morphogenesis
GO:0072136 ISS:UniProtKB P metanephric mesenchymal cell proliferation involved in metanephros development
GO:0001656 ISS:UniProtKB P metanephros development
GO:0030901 ISS:UniProtKB P midbrain development
GO:0043066 IMP:RGD P negative regulation of apoptotic process
GO:0045596 ISS:UniProtKB P negative regulation of cell differentiation
GO:0030336 ISS:UniProtKB P negative regulation of cell migration
GO:2000357 ISS:UniProtKB P negative regulation of kidney smooth muscle cell differentiation
GO:2000062 ISS:UniProtKB P negative regulation of ureter smooth muscle cell differentiation
GO:0045060 ISS:UniProtKB P negative thymic T cell selection
GO:0001755 ISS:UniProtKB P neural crest cell migration
GO:0001841 IMP:RGD P neural tube formation
GO:0007405 ISS:UniProtKB P neuroblast proliferation
GO:0048663 ISS:UniProtKB P neuron fate commitment
GO:0001569 ISS:UniProtKB P patterning of blood vessels
GO:0007389 ISS:UniProtKB P pattern specification process
GO:0009949 ISS:UniProt P polarity specification of anterior/posterior axis
GO:0046638 ISS:UniProtKB P positive regulation of alpha-beta T cell differentiation
GO:0008284 ISS:UniProtKB P positive regulation of cell proliferation
GO:0033092 ISS:UniProtKB P positive regulation of immature T cell proliferation in thymus
GO:2000358 ISS:UniProtKB P positive regulation of kidney smooth muscle cell differentiation
GO:2000729 ISS:UniProtKB P positive regulation of mesenchymal cell proliferation involved in ureter development
GO:0002052 IDA:RGD P positive regulation of neuroblast proliferation
GO:0045666 IMP:RGD P positive regulation of neuron differentiation
GO:0070447 IDA:RGD P positive regulation of oligodendrocyte progenitor proliferation
GO:0060406 IMP:RGD P positive regulation of penile erection
GO:0046534 IDA:RGD P positive regulation of photoreceptor cell differentiation
GO:0045880 ISS:UniProtKB P positive regulation of smoothened signaling pathway
GO:0033089 ISS:UniProtKB P positive regulation of T cell differentiation in thymus
GO:0045893 ISS:UniProtKB P positive regulation of transcription, DNA-templated
GO:2000063 ISS:UniProtKB P positive regulation of ureter smooth muscle cell differentiation
GO:0045059 ISS:UniProtKB P positive thymic T cell selection
GO:0030850 ISS:UniProtKB P prostate gland development
GO:0042127 ISS:UniProtKB P regulation of cell proliferation
GO:0030856 IEP:RGD P regulation of epithelial cell differentiation
GO:0030162 ISS:UniProtKB P regulation of proteolysis
GO:0048678 IMP:RGD P response to axon injury
GO:0032355 IEP:RGD P response to estradiol
GO:0045471 IDA:RGD P response to ethanol
GO:0001666 IEP:RGD P response to hypoxia
GO:0010243 IEP:RGD P response to organonitrogen compound
GO:0032526 IEP:RGD P response to retinoic acid
GO:0007224 ISS:UniProtKB P smoothened signaling pathway
GO:0021513 IDA:MGI P spinal cord dorsal/ventral patterning
GO:0048864 ISS:UniProtKB P stem cell development
GO:0033077 ISS:UniProtKB P T cell differentiation in thymus
GO:0021978 IMP:RGD P telencephalon regionalization
GO:0048538 ISS:UniProtKB P thymus development
GO:0043586 IMP:RGD P tongue development
GO:0043587 IMP:RGD P tongue morphogenesis
GO:0001570 ISS:UniProtKB P vasculogenesis
GO:0021521 IDA:MGI P ventral spinal cord interneuron specification

KEGG Pathway Links

KEGG Pathway ID Description
ko05217 Basal cell carcinoma
rno05217 Basal cell carcinoma
ko04340 Hedgehog signaling pathway
rno04340 Hedgehog signaling pathway
rno05200 Pathways in cancer

REACTOME Pathway Links

REACTOME Pathway ID Description
5953757 Class B/2 (Secretin family receptors)
5953253 Disease
5953758 GPCR ligand binding
5954643 Hedgehog ligand biogenesis
5954646 Hh ligand biogenesis disease
5954645 Processing-defective Hh variants abrogate ligand secretion
5954650 Release of Hh-Np from the secreting cell
5953391 Signaling by GPCR
5954644 Signaling by Hedgehog
5953381 Signal Transduction

Domain Information

InterPro Annotations

Accession Description
IPR028992 Hedgehog/Intein (Hint) domain
IPR000320 Hedgehog, N-terminal signalling domain
IPR001657 Hedgehog protein
IPR001767 Hedgehog protein, Hint domain
IPR009045 Hedgehog signalling/DD-peptidase zinc-binding domain
IPR003586 Hint domain C-terminal
IPR003587 Hint domain N-terminal
IPR006141 Intein splice site

UniProt Annotations

Entry Information

Gene Name
sonic hedgehog
Protein Entry
SHH_RAT
UniProt ID
Q63673
Species
Rat

Comments

Comment Type Description
Domain The sonic hedgehog protein N-product binds calcium and zinc ions; this stabilizes the protein fold and is essential for protein-protein interactions mediated by this domain
Function Intercellular signal essential for a variety of patterning events during development: signal produced by the notochord that induces ventral cell fate in the neural tube and somites, and the polarizing signal for patterning of the anterior- posterior axis of the developing limb bud. Displays both floor plate- and motor neuron-inducing activity. The threshold concentration of N-product required for motor neuron induction is 5-fold lower than that required for floor plate induction. Activates the transcription of target genes by interacting with its receptor PTCH1 to prevent normal inhibition by PTCH1 on the constitutive signaling activity of SMO (By similarity)
Ptm Cholesterylation is required for N-product targeting to lipid rafts and multimerization
Ptm N-palmitoylation of Cys-25 by HHAT is required for N-product multimerization and full activity
Ptm The C-terminal domain displays an autoproteolysis activity and a cholesterol transferase activity. Both activities result in the cleavage of the full-length protein and covalent attachment of a cholesterol moiety to the C-terminal of the newly generated N- terminal fragment (N-product). The N-product is the active species in both local and long-range signaling, whereas the C-product has no signaling activity.
Similarity Belongs to the hedgehog family
Subcellular Location Sonic hedgehog protein C-product: Secreted, extracellular space. Note=The C-terminal peptide diffuses from the cell.
Subcellular Location Sonic hedgehog protein N-product: Cell membrane; Lipid-anchor. Note=The N-product either remains associated with lipid rafts at the cell surface, or forms freely diffusible active multimers with its hydrophobic lipid-modified N- and C-termini buried inside.
Subunit Interacts with HHATL/GUP1 which negatively regulates HHAT-mediated palmitoylation of the SHH N-terminus. Interacts with BOC and CDON. Interacts with HHIP (By similarity). N-product is active as a multimer
Tissue Specificity Expressed in the node, notochord, floor plate, and posterior limb bud mesenchyme.

Identical and Related Proteins

Unique RefSeq proteins for LMP012598 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
8394267 RefSeq NP_058917 437 sonic hedgehog protein precursor

Identical Sequences to LMP012598 proteins

Reference Database Accession Length Protein Name
GI:8394267 GenBank AAE72529.1 437 Sequence 9 from patent US 6235885
GI:8394267 GenBank AAQ31790.1 437 Sequence 2 from patent US 6566092
GI:8394267 GenBank ADL91051.1 437 Sequence 64 from patent US 7709454
GI:8394267 GenBank ADR89539.1 437 Sequence 6 from patent US 7741298

Related Sequences to LMP012598 proteins

Reference Database Accession Length Protein Name
GI:8394267 GenBank AAE72526.1 437 Sequence 2 from patent US 6235885
GI:8394267 GenBank AAE72529.1 437 Sequence 9 from patent US 6235885
GI:8394267 GenBank AAQ31790.1 437 Sequence 2 from patent US 6566092