Gene/Proteome Database (LMPD)

LMPD ID
LMP012628
Gene ID
29725
Species
Rattus norvegicus (Rat)
Gene Name
bile acid CoA:amino acid N-acyltransferase
Gene Symbol
Baat
Synonyms
kan-1;
Alternate Names
bile acid-CoA:amino acid N-acyltransferase; BAT; BACAT; glycine N-choloyltransferase; long-chain fatty-acyl-CoA hydrolase; bile acid-Coenzyme A: amino acid N-acyltransferase; bile acid-Coenzyme A dehydrogenase: amino acid n-acyltransferase; bile acid CoA: amino acid N-acyltransferase (glycine N-choloyltransferase); bile acid Coenzyme A: amino acid N-acyltransferase (glycine N-choloyltransferase);
Chromosome
5
Map Location
5q22
EC Number
2.3.1.65
Summary
may play a role in acute phase response [RGD, Feb 2006]
Orthologs
View orthologs and multiple alignments for Baat

Proteins

bile acid-CoA:amino acid N-acyltransferase
Refseq ID NP_058996
Protein GI 148747510
UniProt ID Q63276
mRNA ID NM_017300
Length 420
MAKLTAVPLSALVDEPVHIRVTGLTPFQVVCLQASLKDDKGNLFNSQAFYRASEVGEVDLERDSSLGGDYMGVHPMGLFWSMKPEKLLTRLVKRDVMNRPHKVHIKLCHPYFPVEGKVISSSLDSLILERWYVAPGVTRIHVKEGRIRGALFLPPGEGPFPGVIDLFGGAGGLFEFRASLLASHGFATLALAYWGYDDLPSRLEKVDLEYFEEGVEFLLRHPKVLGPGVGILSVCIGAEIGLSMAINLKQITATVLINGPNFVSSNPHVYRGKVFQPTPCSEEFVTTNALGLVEFYRTFEETADKDSKYCFPIEKAHGHFLFVVGEDDKNLNSKVHAKQAIAQLMKSGKKNWTLLSYPGAGHLIEPPYSPLCSASRMPFVIPSINWGGEVIPHAAAQEHSWKEIQKFLKQHLNPGFNSQL

Gene Information

Entrez Gene ID
29725
Gene Name
bile acid CoA:amino acid N-acyltransferase
Gene Symbol
Baat
Species
Rattus norvegicus

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005829 IEA:Ensembl C cytosol
GO:0005777 IDA:HGNC C peroxisome
GO:0052689 IEA:UniProtKB-KW F carboxylic ester hydrolase activity
GO:0047963 IEA:UniProtKB-EC F glycine N-choloyltransferase activity
GO:0052815 IEA:Ensembl F medium-chain acyl-CoA hydrolase activity
GO:0016410 IEA:Ensembl F N-acyltransferase activity
GO:0016290 IEA:UniProtKB-EC F palmitoyl-CoA hydrolase activity
GO:0052817 IEA:Ensembl F very long chain acyl-CoA hydrolase activity
GO:0006637 IEA:Ensembl P acyl-CoA metabolic process
GO:0006699 IEA:Ensembl P bile acid biosynthetic process
GO:0002152 IEA:Ensembl P bile acid conjugation
GO:0006631 IEA:UniProtKB-KW P fatty acid metabolic process
GO:0006544 IEA:Ensembl P glycine metabolic process
GO:0001889 IEP:RGD P liver development
GO:0031100 IEP:RGD P organ regeneration
GO:0019530 IEA:Ensembl P taurine metabolic process

KEGG Pathway Links

KEGG Pathway ID Description
ko04976 Bile secretion
rno04976 Bile secretion
ko01040 Biosynthesis of unsaturated fatty acids
rno01040 Biosynthesis of unsaturated fatty acids
M00106 Conjugated bile acid biosynthesis, cholate => taurocholate/glycocholate
rno01100 Metabolic pathways
ko04146 Peroxisome
rno04146 Peroxisome
ko00120 Primary bile acid biosynthesis
rno00120 Primary bile acid biosynthesis
ko00430 Taurine and hypotaurine metabolism
rno00430 Taurine and hypotaurine metabolism

BIOCYC Pathway Links

BIOCYC Pathway ID Description
PWY-6061 bile acid biosynthesis, neutral pathway

REACTOME Pathway Links

REACTOME Pathway ID Description
5953720 Bile acid and bile salt metabolism
5953250 Metabolism
5953289 Metabolism of lipids and lipoproteins
5953719 Recycling of bile acids and salts
5953930 Synthesis of bile acids and bile salts
5953931 Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol

Domain Information

InterPro Annotations

Accession Description
IPR016662 Acyl-CoA thioesterase, long chain
IPR006862 Acyl-CoA thioester hydrolase/bile acid-CoA amino acid N-acetyltransferase
IPR029058 Alpha/Beta hydrolase fold
IPR014940 BAAT/Acyl-CoA thioester hydrolase C-terminal

UniProt Annotations

Entry Information

Gene Name
bile acid CoA:amino acid N-acyltransferase
Protein Entry
BAAT_RAT
UniProt ID
Q63276
Species
Rat

Comments

Comment Type Description
Biophysicochemical Properties Kinetic parameters: KM=2.0 mM for taurine toward choloyl-CoA; KM=4.4 mM for glycine toward choloyl-CoA;
Catalytic Activity Choloyl-CoA + glycine = CoA + glycocholate.
Catalytic Activity Palmitoyl-CoA + H(2)O = CoA + palmitate.
Function Involved in bile acid metabolism. In liver hepatocytes catalyzes the second step in the conjugation of C24 bile acids (choloneates) to glycine and taurine before excretion into bile canaliculi. The major components of bile are cholic acid and chenodeoxycholic acid. In a first step the bile acids are converted to an acyl-CoA thioester, either in peroxisomes (primary bile acids deriving from the cholesterol pathway), or cytoplasmic at the endoplasmic reticulum (secondary bile acids). May catalyze the conjugation of primary or secondary bile acids, or both. The conjugation increases the detergent properties of bile acids in the intestine, which facilitates lipid and fat-soluble vitamin absorption. In turn, bile acids are deconjugated by bacteria in the intestine and are recycled back to the liver for reconjugation (secondary bile acids). May also act as an acyl-CoA thioesterase that regulates intracellular levels of free fatty acids. In vitro, catalyzes the hydrolysis of long- and very long-chain saturated acyl-CoAs to the free fatty acid and coenzyme A (CoASH), and conjugates glycine to these acyl-CoAs (By similarity)
Miscellaneous Rat BAAT seems to be more efficient at taurine conjugation of cholyl CoA than glycine conjugation. In rat the relative amounts of glycine- and taurine-conjugated bile acids formed in bile are approximately in a ratio of 1:6.
Similarity Belongs to the C/M/P thioester hydrolase family
Subcellular Location Peroxisome .
Subunit Monomer
Tissue Specificity Expressed in liver (at protein level); found in hepatocytes, sinusoidal endothelial cells and Kupffer cells

Identical and Related Proteins

Unique RefSeq proteins for LMP012628 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
148747510 RefSeq NP_058996 420 bile acid-CoA:amino acid N-acyltransferase

Identical Sequences to LMP012628 proteins

Reference Database Accession Length Protein Name
GI:148747510 GenBank AAH88153.1 420 Bile acid Coenzyme A: amino acid N-acyltransferase (glycine N-choloyltransferase) [Rattus norvegicus]
GI:148747510 GenBank EDL78183.1 420 bile acid-Coenzyme A: amino acid N-acyltransferase [Rattus norvegicus]
GI:148747510 SwissProt Q63276.2 420 RecName: Full=Bile acid-CoA:amino acid N-acyltransferase; Short=BACAT; Short=BAT; AltName: Full=Glycine N-choloyltransferase; AltName: Full=Kan-1; AltName: Full=Long-chain fatty-acyl-CoA hydrolase [Rattus norvegicus]

Related Sequences to LMP012628 proteins

Reference Database Accession Length Protein Name
GI:148747510 GenBank AAH88153.1 420 Bile acid Coenzyme A: amino acid N-acyltransferase (glycine N-choloyltransferase) [Rattus norvegicus]
GI:148747510 GenBank EDL78183.1 420 bile acid-Coenzyme A: amino acid N-acyltransferase [Rattus norvegicus]
GI:148747510 SwissProt Q63276.2 420 RecName: Full=Bile acid-CoA:amino acid N-acyltransferase; Short=BACAT; Short=BAT; AltName: Full=Glycine N-choloyltransferase; AltName: Full=Kan-1; AltName: Full=Long-chain fatty-acyl-CoA hydrolase [Rattus norvegicus]