Gene/Proteome Database (LMPD)
LMPD ID
LMP012722
Gene ID
Species
Rattus norvegicus (Rat)
Gene Name
epoxide hydrolase 2, cytoplasmic
Gene Symbol
Alternate Names
bifunctional epoxide hydrolase 2; CEH; SEH; epoxide hydratase; soluble epoxide hydrolase; cytosolic epoxide hydrolase;
Chromosome
15
Map Location
15p12
EC Number
3.3.2.10
Summary
catalyzes the hydrolysis of an epoxide to a glycol; plays a role in regulation of blood pressure [RGD, Feb 2006]
Orthologs
Proteins
| bifunctional epoxide hydrolase 2 | |
|---|---|
| Refseq ID | NP_075225 |
| Protein GI | 12621098 |
| UniProt ID | P80299 |
| mRNA ID | NM_022936 |
| Length | 554 |
| MALRVAAFDLDGVLALPSIAGVLRHTEEALALPRDFLLGAFQMKFPEGPTEQLMKGKITFSQWVPLMDESCRKSSKACGASLPENFSISEIFSQAMAARSINRPMLQAAAALKKKGFTTCIVTNNWLDDSDKRDILAQMMCELSQHFDFLIESCQVGMIKPEPQIYKFVLDTLKAKPNEVVFLDDFGSNLKPARDMGMVTILVRDTASALRELEKVTGTQFPEAPLPVPCSPNDVSHGYVTVKPGIRLHFVEMGSGPAICLCHGFPESWFSWRYQIPALAQAGFRVLAIDMKGYGDSSSPPEIEEYAMELLCEEMVTFLNKLGIPQAVFIGHDWAGVLVWNMALFHPERVRAVASLNTPLMPPNPEVSPMEVIRSIPVFNYQLYFQEPGVAEAELEKNMSRTFKSFFRTSDDMGLLTVNKATEMGGILVGTPEDPKVSKITTEEEIEYYIQQFKKSGFRGPLNWYRNTERNWKWSCKALGRKILVPALMVTAEKDIVLRPEMSKNMENWIPFLKRGHIEDCGHWTQIEKPAEVNQILIKWLKTEIQNPSVTSKI | |
Gene Information
Entrez Gene ID
Gene Name
epoxide hydrolase 2, cytoplasmic
Gene Symbol
Species
Rattus norvegicus
Gene Ontology (GO Annotations)
| GO ID | Source | Type | Description |
|---|---|---|---|
| GO:0005829 | IDA:RGD | C | cytosol |
| GO:0005777 | IDA:RGD | C | peroxisome |
| GO:0033885 | IEA:UniProtKB-EC | F | 10-hydroxy-9-(phosphonooxy)octadecanoate phosphatase activity |
| GO:0004301 | IDA:RGD | F | epoxide hydrolase activity |
| GO:0042577 | ISS:UniProtKB | F | lipid phosphatase activity |
| GO:0000287 | IMP:RGD | F | magnesium ion binding |
| GO:0016791 | IDA:RGD | F | phosphatase activity |
| GO:0019439 | IEA:UniProtKB-KW | P | aromatic compound catabolic process |
| GO:0006954 | IMP:RGD | P | inflammatory response |
| GO:0043651 | IMP:RGD | P | linoleic acid metabolic process |
| GO:0046839 | ISS:UniProtKB | P | phospholipid dephosphorylation |
| GO:0045777 | IMP:RGD | P | positive regulation of blood pressure |
| GO:0002539 | IMP:RGD | P | prostaglandin production involved in inflammatory response |
| GO:0009636 | IEA:UniProtKB-KW | P | response to toxic substance |
| GO:0019233 | IMP:RGD | P | sensory perception of pain |
KEGG Pathway Links
| KEGG Pathway ID | Description |
|---|---|
| ko00590 | Arachidonic acid metabolism |
| rno00590 | Arachidonic acid metabolism |
| rno01100 | Metabolic pathways |
| ko04146 | Peroxisome |
| rno04146 | Peroxisome |
REACTOME Pathway Links
Domain Information
InterPro Annotations
| Accession | Description |
|---|---|
| IPR029058 | Alpha/Beta hydrolase fold |
| IPR000073 | Alpha/beta hydrolase fold-1 |
| IPR000639 | Epoxide hydrolase-like |
| IPR006439 | HAD hydrolase, subfamily IA |
| IPR023214 | HAD-like domain |
| IPR023198 | Phosphoglycolate phosphatase, domain 2 |
| IPR011945 | Predicted HAD-superfamily phosphatase, subfamily IA/Epoxide hydrolase, N-terminal |
UniProt Annotations
Entry Information
Comments
| Comment Type | Description |
|---|---|
| Biophysicochemical Properties | Kinetic parameters: KM=0.235 mM for p-nitrophenyl phosphate; Vmax=1450 nmol/min/mg enzyme; |
| Catalytic Activity | (9S,10S)-10-hydroxy-9- (phosphonooxy)octadecanoate + H(2)O = (9S,10S)-9,10- dihydroxyoctadecanoate + phosphate. |
| Catalytic Activity | An epoxide + H(2)O = a glycol. |
| Cofactor | Name=Mg(2+); Xref=ChEBI:CHEBI:18420; |
| Domain | The N-terminal domain has phosphatase activity. The C- terminal domain has epoxide hydrolase activity. |
| Function | Bifunctional enzyme. The C-terminal domain has epoxide hydrolase activity and acts on epoxides (alkene oxides, oxiranes) and arene oxides. Plays a role in xenobiotic metabolism by degrading potentially toxic epoxides. Also determines steady-state levels of physiological mediators. The N-terminal domain has lipid phosphatase activity, with the highest activity towards threo- 9,10-phosphonooxy-hydroxy-octadecanoic acid, followed by erythro- 9,10-phosphonooxy-hydroxy-octadecanoic acid, 12-phosphonooxy- octadec-9Z-enoic acid, 12-phosphonooxy-octadec-9E-enoic acid, and p-nitrophenyl phospate. |
| Induction | By compounds that cause peroxisome proliferation such as clofibrate, tiadenol and fenofibrate. |
| Ptm | The covalent modification of cysteine by 15-deoxy-Delta12,14- prostaglandin-J2 is autocatalytic and reversible. It may occur as an alternative to other cysteine modifications, such as S- nitrosylation and S-palmitoylation (By similarity) |
| Similarity | Belongs to the AB hydrolase superfamily. Epoxide hydrolase family |
| Subcellular Location | Cytoplasm. Peroxisome. |
| Subunit | Homodimer. |
Identical and Related Proteins
Unique RefSeq proteins for LMP012722 (as displayed in Record Overview)
| Protein GI | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| 12621098 | RefSeq | NP_075225 | 554 | bifunctional epoxide hydrolase 2 |
Identical Sequences to LMP012722 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:12621098 | GenBank | ADF11199.1 | 554 | Sequence 2 from patent US 7662910 |
| GI:12621098 | GenBank | AEQ78411.1 | 554 | Sequence 40 from patent US 8030048 |
| GI:12621098 | GenBank | AGN61908.1 | 554 | Sequence 2 from patent US 8455652 |
| GI:12621098 | GenBank | AGU04325.1 | 554 | Sequence 2 from patent US 8476043 |
Related Sequences to LMP012722 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:12621098 | EMBL | CAA46211.1 | 554 | epoxide hydrolase [Rattus norvegicus] |
| GI:12621098 | GenBank | ADF11199.1 | 554 | Sequence 2 from patent US 7662910 |
| GI:12621098 | GenBank | AGN61908.1 | 554 | Sequence 2 from patent US 8455652 |