Gene/Proteome Database (LMPD)
LMPD ID
LMP012731
Gene ID
Species
Rattus norvegicus (Rat)
Gene Name
ArfGAP with GTPase domain, ankyrin repeat and PH domain 2
Gene Symbol
Synonyms
Pike; Centg1;
Alternate Names
arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2; AGAP-2; cnt-g1; centaurin-gamma-1; centaurin, gamma 1; nuclear GTPase PIKE; phosphatidylinositol 3-kinase enhancer; phosphatidylinositol-3-kinase enhancer;
Chromosome
7
Map Location
7q22
Summary
a nuclear GTPase that interacts with PI3K [RGD, Feb 2006]
Orthologs
Proteins
| arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2 | |
|---|---|
| Refseq ID | NP_075415 |
| Protein GI | 259155312 |
| UniProt ID | Q8CGU4 |
| mRNA ID | NM_023026 |
| Length | 1186 |
| MSRGAGALQRRTTTYLISLTLVKLESVPPPPPSPSAAAVGAPGARGSEPRDPGSPRGAEEPGKKRHERLFHRQDALWISTSSAGAGGAEPPALSPAPASPARPVSPAPGRRLSLWAAPPGPPLSGGLSPDSKPGGAPSSSRRPLLSSPSWGGPEPEGRTGGGVPGSSSPHPGTGSRRLKVAPPPPAPKPFKTVTTSGAKAGGGKGAGSRLSWPESEGKPRVKGSKSTAGTGASAVAAGGGGSAAVTTSGGVGAGAGARGKLSPRKGKSKTLDNSDLHPGPSAGSPPLTVPAIPVPATSVTAASTQPLGPAPPITLEPPAPGLKRGREGGRASTRDRKMLKFISGIFTKSTGGPPGPGPLPGPQGLSSSSGSRELLGAELRASPKAVVNSQEWTLSRSIPELRLGVLGDVRSGKSSLIHRFLTGSYQVLEKPESEQYKKEMLVDGQTHLVLIREEAGAPDAKFSGWADAVIFVFSLEDESSFQAVSHLHGQLISLRGEGRGGLALALVGTQDRISASSPRVVGDARARALCTDMKRCSYYETCATYGLNVDRVFQEVAQKVVTLRKQQQLLAACKSLPSSPSHSAASTPVAGQASNGGHTSDYSSSLPSSPNVGHRELRAEAAAVAGLSTPGSLHRAAKRRTSLFANRRGSDSEKRSLDSRGETTGSGRAIPIKQSFLLKRSGNSLNKEWKKKYVTLSSNGFLLYHPSINDYIHSTHGKEMDLLRTTVKVPGKRPPRAISAFGPSASINGLVKDMSTVQMGEGPEASTPMPSPSPSPSSLQLPTDQTSKHLLKPDRNLARALSTDCTPSGDLSPLSREPPPSPMVKKQRRKKLSTPSKTEGSAVQAEAKRKMWKLKSFGSLRNIYKAEENFEFLIVSSTGQTWHFEAASFEERDAWVQAIESQILASLQCCESSKVKLRTDSQSEAVAIQAIRNAKGNSTCVDCGAPNPTWASLNLGALICIECSGIHRNLGTHLSRVRSLDLDDWPRELTLVLTAIGNDTANRVWESDTRGRAKPTRDSSREERESWIRAKYEQLLFLAPLGTTEEPLGRQLWAAVEAQDVAAVLLLLAHARHGPLDTSVEDPQLRSPLHLAAELAHVVITQLLLWYGADVAARDAQGRTALFYARQAGSQLCADILLQHGCPGEGGSTATTPSAATTPSITATPSPRRRSSAASLGRVDTTIALV | |
Gene Information
Entrez Gene ID
Gene Name
ArfGAP with GTPase domain, ankyrin repeat and PH domain 2
Gene Symbol
Species
Rattus norvegicus
Gene Ontology (GO Annotations)
| GO ID | Source | Type | Description |
|---|---|---|---|
| GO:0005829 | TAS:Reactome | C | cytosol |
| GO:0005739 | IEA:Ensembl | C | mitochondrion |
| GO:0005730 | IEA:Ensembl | C | nucleolus |
| GO:0005634 | IDA:RGD | C | nucleus |
| GO:0008060 | IEA:InterPro | F | ARF GTPase activator activity |
| GO:0005525 | IEA:UniProtKB-KW | F | GTP binding |
| GO:0030295 | IDA:RGD | F | protein kinase activator activity |
| GO:0019901 | IDA:RGD | F | protein kinase binding |
| GO:0008270 | IEA:InterPro | F | zinc ion binding |
| GO:0060749 | IEA:Ensembl | P | mammary gland alveolus development |
| GO:2001240 | IMP:UniProtKB | P | negative regulation of extrinsic apoptotic signaling pathway in absence of ligand |
| GO:0043524 | IMP:UniProtKB | P | negative regulation of neuron apoptotic process |
| GO:0046427 | IEA:Ensembl | P | positive regulation of JAK-STAT cascade |
| GO:0033601 | IEA:Ensembl | P | positive regulation of mammary gland epithelial cell proliferation |
| GO:0014068 | IMP:UniProtKB | P | positive regulation of phosphatidylinositol 3-kinase signaling |
| GO:0045860 | IDA:GOC | P | positive regulation of protein kinase activity |
| GO:0045944 | IEA:Ensembl | P | positive regulation of transcription from RNA polymerase II promoter |
| GO:0032312 | IEA:InterPro | P | regulation of ARF GTPase activity |
| GO:0007264 | IEA:InterPro | P | small GTPase mediated signal transduction |
KEGG Pathway Links
| KEGG Pathway ID | Description |
|---|---|
| ko04144 | Endocytosis |
| rno04144 | Endocytosis |
| rno04068 | FoxO signaling pathway |
REACTOME Pathway Links
Domain Information
InterPro Annotations
| Accession | Description |
|---|---|
| IPR002110 | Ankyrin repeat |
| IPR020683 | Ankyrin repeat-containing domain |
| IPR001164 | Arf GTPase activating protein |
| IPR013684 | Mitochondrial Rho-like |
| IPR001849 | Pleckstrin homology domain |
| IPR011993 | Pleckstrin homology-like domain |
| IPR027417 | P-loop containing nucleoside triphosphate hydrolase |
UniProt Annotations
Entry Information
Gene Name
ArfGAP with GTPase domain, ankyrin repeat and PH domain 2
Protein Entry
AGAP2_RAT
UniProt ID
Species
Rat
Comments
| Comment Type | Description |
|---|---|
| Domain | Arf-GAP domain interacts with G domain and may regulate its GTPase activity |
| Domain | G domain binds GTP and has GTPase activity |
| Domain | PH domain binds phospholipids and is required for nuclear targeting |
| Enzyme Regulation | GAP activity is stimulated by phosphatidylinositol 4,5-bisphosphate (PIP2) and, to a lesser extent, by phosphatidylinositol 3,4,5-trisphosphate (PIP3). Phosphatidic acid potentiates PIP2 stimulation (By similarity) |
| Function | GTPase-activating protein (GAP) for ARF1 and ARF5, which also shows strong GTPase activity. Participates in the prevention of neuronal apoptosis by enhancing PI3 kinase activity. Aids the coupling of metabotropic glutamate receptor 1 (GRM1) to cytoplasmic PI3 kinase by interacting with Homer scaffolding proteins, and also seems to mediate anti-apoptotic effects of NGF by activating nuclear PI3 kinase. {ECO:0000269|PubMed:11136977, ECO:0000269|PubMed:11823862, ECO:0000269|PubMed:15385964, ECO:0000269|PubMed:16263930}. |
| Interaction | Q63155:Dcc; NbExp=2; IntAct=EBI-4409108, EBI-1798965; P19491:Gria2; NbExp=4; IntAct=EBI-4409108, EBI-77718; P97879:Grip1; NbExp=4; IntAct=EBI-4409108, EBI-936113; Q9Z214-1:Homer1; NbExp=4; IntAct=EBI-4409108, EBI-4410552; Q8IZJ1:UNC5B (xeno); NbExp=9; IntAct=EBI-4409108, EBI-4409075; O08722:Unc5b; NbExp=6; IntAct=EBI-4409108, EBI-4404185; |
| Sequence Caution | Sequence=AAF97595.1; Type=Frameshift; Positions=Several; Note=This sequence would represent a shorter strictly nuclear isoform named PIKE-S. It may be an artifactual frameshifted form whose existence in vivo is dubious.; Evidence= ; |
| Similarity | Belongs to the centaurin gamma-like family |
| Similarity | Contains 1 Arf-GAP domain. {ECO:0000255|PROSITE- ProRule:PRU00288}. |
| Similarity | Contains 1 PH domain. {ECO:0000255|PROSITE- ProRule:PRU00145}. |
| Similarity | Contains 2 ANK repeats. {ECO:0000255|PROSITE- ProRule:PRU00023}. |
| Subcellular Location | Cytoplasm. Nucleus. |
| Subunit | Interacts with EPB41L1, PLCG1, NF2, HOMER1 and HOMER2. {ECO:0000269|PubMed:11136977, ECO:0000269|PubMed:11823862, ECO:0000269|PubMed:14528310, ECO:0000269|PubMed:15598747}. |
| Tissue Specificity | Present in cortex, hippocampus and olfactory bulb but absent in cerebellum (at protein level) |
Identical and Related Proteins
Unique RefSeq proteins for LMP012731 (as displayed in Record Overview)
| Protein GI | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| 259155312 | RefSeq | NP_075415 | 1186 | arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2 |
Identical Sequences to LMP012731 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:259155312 | GenBank | AAM97539.1 | 1186 | PI 3-kinase enhancer long isoform, partial [Rattus norvegicus] |
| GI:259155312 | GenBank | EDM16498.1 | 1186 | centaurin, gamma 1, partial [Rattus norvegicus] |
| GI:259155312 | SwissProt | Q8CGU4.1 | 1186 | RecName: Full=Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2; Short=AGAP-2; AltName: Full=Centaurin-gamma-1; Short=Cnt-g1; AltName: Full=Phosphatidylinositol 3-kinase enhancer; Short=PIKE [Rattus norvegicus] |
Related Sequences to LMP012731 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:259155312 | GenBank | AAM97539.1 | 1186 | PI 3-kinase enhancer long isoform, partial [Rattus norvegicus] |
| GI:259155312 | GenBank | EDM16498.1 | 1186 | centaurin, gamma 1, partial [Rattus norvegicus] |
| GI:259155312 | SwissProt | Q8CGU4.1 | 1186 | RecName: Full=Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2; Short=AGAP-2; AltName: Full=Centaurin-gamma-1; Short=Cnt-g1; AltName: Full=Phosphatidylinositol 3-kinase enhancer; Short=PIKE [Rattus norvegicus] |