Gene/Proteome Database (LMPD)

LMPD ID
LMP012737
Gene ID
78968
Species
Rattus norvegicus (Rat)
Gene Name
sterol regulatory element binding transcription factor 1
Gene Symbol
Srebf1
Synonyms
ADD1; ADD-1; Srebp1; SREBP-1; SREBP-1c;
Alternate Names
sterol regulatory element-binding protein 1; adipocyte determination- and differentiation-dependent factor 1;
Chromosome
10
Map Location
10q22
Summary
transcription factor; binds to the sterol regulatory element 1; regulates the transcription of genes important for sterol biosynthesis [RGD, Feb 2006]
Orthologs
View orthologs and multiple alignments for Srebf1

Proteins

sterol regulatory element-binding protein 1 isoform 1 precursor
Refseq ID NP_001263636
Protein GI 453040303
UniProt ID P56720
mRNA ID NM_001276707
Length 1134
MDELPFGEAALEQALAEVCEMDAALLTDIEDMLQLINNQDSDFPGLFDAPYAGGETGDTGPSSPGASSPESFSSPASLGSSLEAFLGGPKVTPAPLSPPPSAPTAVKMYPSVPPFSPGPGIKEEPVPLTILQPPAPQPSPGTLLPPSFPPPPVQLSPAPVLGYSSLPSGFSGTLPGNTQQTPSSLPLGSTPGISPTPLHTQVQSSAAQQPPPASAAPRMSTVASQIQQVPVVLQPHFIKADSLLLTAVKTDTGATMKTAGINTLAPGTAVQAGPLQTLVSGGTILATVPLVVDTDKLPIHRLAAGGKALGSAQSRGEKRTAHNAIEKRYRSSINDKIVELKDLVVGTEAKLNKSAVLRKAIDYIRFLQHSNQKLKQENLTLRSAHKSKSLKDLVSACGSGGGTDVSMEGMKPEVVETLTPPPSDAGSPSQSSPLSLGSRGSSSGGSDSEPDSPAFEDNQVKAQRLPSHSRGMLDRSRLALCVLVFLCLTCNPLASLFGWGILTPSDASGVHRSSGRSMLEAESRDGSNWTQWLLPPLVWLANGLLVLACLALLFVYGEPVTRPHSGPAVHFWRHRKQADLDLARGDFAQAAQQLWLALQALGRPLPTSNLDLACSLLWNLVRHLLQRLWVGRWLAGQAGGLQRDYRLRKDARASARDAAVVYHKLHQLHAMGKYTGGHLVASNLALSALNLAECAGDAISMATLAEIYVAAALRVKTSLPRALHFLTRFFLSSARQACLAQSGAVPLAMQWLCHPVGHRFFVDGDWAVHGAPQESLYSVAGNPVDPLAQVTRLFCEHLLERALNCIAQPSPGAADGDREFSDALGYLQLLNSCSDAVGAPACSFSVSSSMATTTGTDPVAKWWASLTAVVIHWLRRDEEAAERLYPLVEHIPQVLQETERPLPRAALYSFKAARALLDHRKVESSPASLAICEKASGYLRDSLASTSTASSIDKAMQLLLCDLLLVARTSLWRRQQSAASAQGAHGTSNGPQASALELRGFQHDLSSLRRLAQSFRPAMRRVFLHEATARLMAGASPARTHQLLDRSLRRRAGSSGKGGAAAELEPRPTWREHTEALLLASCYLPPAFLSAPGQRVSMLAEAARTVEKLGDHRLLLDCQQMLLRLGGGTTVTSS
mat_peptide: 1..480 product: Processed sterol regulatory element-binding protein 1 experiment: experimental evidence, no additional details recorded note: propagated from UniProtKB/Swiss-Prot (P56720.3) calculated_mol_wt: 49463 peptide sequence: MDELPFGEAALEQALAEVCEMDAALLTDIEDMLQLINNQDSDFPGLFDAPYAGGETGDTGPSSPGASSPESFSSPASLGSSLEAFLGGPKVTPAPLSPPPSAPTAVKMYPSVPPFSPGPGIKEEPVPLTILQPPAPQPSPGTLLPPSFPPPPVQLSPAPVLGYSSLPSGFSGTLPGNTQQTPSSLPLGSTPGISPTPLHTQVQSSAAQQPPPASAAPRMSTVASQIQQVPVVLQPHFIKADSLLLTAVKTDTGATMKTAGINTLAPGTAVQAGPLQTLVSGGTILATVPLVVDTDKLPIHRLAAGGKALGSAQSRGEKRTAHNAIEKRYRSSINDKIVELKDLVVGTEAKLNKSAVLRKAIDYIRFLQHSNQKLKQENLTLRSAHKSKSLKDLVSACGSGGGTDVSMEGMKPEVVETLTPPPSDAGSPSQSSPLSLGSRGSSSGGSDSEPDSPAFEDNQVKAQRLPSHSRGMLDRSRLAL
sterol regulatory element-binding protein 1 isoform 2
Refseq ID NP_001263637
Protein GI 453040314
UniProt ID P56720
mRNA ID NM_001276708
Length 1110
MDCTFEDMLQLINNQDSDFPGLFDAPYAGGETGDTGPSSPGASSPESFSSPASLGSSLEAFLGGPKVTPAPLSPPPSAPTAVKMYPSVPPFSPGPGIKEEPVPLTILQPPAPQPSPGTLLPPSFPPPPVQLSPAPVLGYSSLPSGFSGTLPGNTQQTPSSLPLGSTPGISPTPLHTQVQSSAAQQPPPASAAPRMSTVASQIQQVPVVLQPHFIKADSLLLTAVKTDTGATMKTAGINTLAPGTAVQAGPLQTLVSGGTILATVPLVVDTDKLPIHRLAAGGKALGSAQSRGEKRTAHNAIEKRYRSSINDKIVELKDLVVGTEAKLNKSAVLRKAIDYIRFLQHSNQKLKQENLTLRSAHKSKSLKDLVSACGSGGGTDVSMEGMKPEVVETLTPPPSDAGSPSQSSPLSLGSRGSSSGGSDSEPDSPAFEDNQVKAQRLPSHSRGMLDRSRLALCVLVFLCLTCNPLASLFGWGILTPSDASGVHRSSGRSMLEAESRDGSNWTQWLLPPLVWLANGLLVLACLALLFVYGEPVTRPHSGPAVHFWRHRKQADLDLARGDFAQAAQQLWLALQALGRPLPTSNLDLACSLLWNLVRHLLQRLWVGRWLAGQAGGLQRDYRLRKDARASARDAAVVYHKLHQLHAMGKYTGGHLVASNLALSALNLAECAGDAISMATLAEIYVAAALRVKTSLPRALHFLTRFFLSSARQACLAQSGAVPLAMQWLCHPVGHRFFVDGDWAVHGAPQESLYSVAGNPVDPLAQVTRLFCEHLLERALNCIAQPSPGAADGDREFSDALGYLQLLNSCSDAVGAPACSFSVSSSMATTTGTDPVAKWWASLTAVVIHWLRRDEEAAERLYPLVEHIPQVLQETERPLPRAALYSFKAARALLDHRKVESSPASLAICEKASGYLRDSLASTSTASSIDKAMQLLLCDLLLVARTSLWRRQQSAASAQGAHGTSNGPQASALELRGFQHDLSSLRRLAQSFRPAMRRVFLHEATARLMAGASPARTHQLLDRSLRRRAGSSGKGGAAAELEPRPTWREHTEALLLASCYLPPAFLSAPGQRVSMLAEAARTVEKLGDHRLLLDCQQMLLRLGGGTTVTSS
mat_peptide: 1..480 product: Processed sterol regulatory element-binding protein 1 experiment: experimental evidence, no additional details recorded note: propagated from UniProtKB/Swiss-Prot (P56720.3) calculated_mol_wt: 49463 peptide sequence: MDELPFGEAALEQALAEVCEMDAALLTDIEDMLQLINNQDSDFPGLFDAPYAGGETGDTGPSSPGASSPESFSSPASLGSSLEAFLGGPKVTPAPLSPPPSAPTAVKMYPSVPPFSPGPGIKEEPVPLTILQPPAPQPSPGTLLPPSFPPPPVQLSPAPVLGYSSLPSGFSGTLPGNTQQTPSSLPLGSTPGISPTPLHTQVQSSAAQQPPPASAAPRMSTVASQIQQVPVVLQPHFIKADSLLLTAVKTDTGATMKTAGINTLAPGTAVQAGPLQTLVSGGTILATVPLVVDTDKLPIHRLAAGGKALGSAQSRGEKRTAHNAIEKRYRSSINDKIVELKDLVVGTEAKLNKSAVLRKAIDYIRFLQHSNQKLKQENLTLRSAHKSKSLKDLVSACGSGGGTDVSMEGMKPEVVETLTPPPSDAGSPSQSSPLSLGSRGSSSGGSDSEPDSPAFEDNQVKAQRLPSHSRGMLDRSRLAL

Gene Information

Entrez Gene ID
78968
Gene Name
sterol regulatory element binding transcription factor 1
Gene Symbol
Srebf1
Species
Rattus norvegicus

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005794 IEA:UniProtKB-KW C Golgi apparatus
GO:0005737 ISS:UniProtKB C cytoplasm
GO:0031410 IEA:UniProtKB-KW C cytoplasmic vesicle
GO:0005783 IEA:UniProtKB-KW C endoplasmic reticulum
GO:0016021 IEA:UniProtKB-KW C integral component of membrane
GO:0043231 IDA:RGD C intracellular membrane-bounded organelle
GO:0005634 IDA:RGD C nucleus
GO:0043234 IDA:RGD C protein complex
GO:0003677 ISS:UniProtKB F DNA binding
GO:0003682 IDA:RGD F chromatin binding
GO:0032403 IPI:RGD F protein complex binding
GO:0043565 IDA:RGD F sequence-specific DNA binding
GO:0003700 ISS:HGNC F sequence-specific DNA binding transcription factor activity
GO:0032810 ISS:HGNC F sterol response element binding
GO:0007568 IEP:RGD P aging
GO:0071398 IEP:RGD P cellular response to fatty acid
GO:0032869 IEP:RGD P cellular response to insulin stimulus
GO:0009267 IDA:HGNC P cellular response to starvation
GO:0008203 IMP:RGD P cholesterol metabolic process
GO:0007623 IEA:Ensembl P circadian rhythm
GO:0008286 IEA:Ensembl P insulin receptor signaling pathway
GO:0008610 ISS:UniProtKB P lipid biosynthetic process
GO:0030324 IEP:RGD P lung development
GO:0046676 IEA:Ensembl P negative regulation of insulin secretion
GO:0000122 IEA:Ensembl P negative regulation of transcription from RNA polymerase II promoter
GO:0045542 IEA:Ensembl P positive regulation of cholesterol biosynthetic process
GO:0031065 IEA:Ensembl P positive regulation of histone deacetylation
GO:0045944 IEA:Ensembl P positive regulation of transcription from RNA polymerase II promoter
GO:0045893 IMP:RGD P positive regulation of transcription, DNA-templated
GO:0010867 ISS:UniProtKB P positive regulation of triglyceride biosynthetic process
GO:0019217 IEA:Ensembl P regulation of fatty acid metabolic process
GO:0003062 IEA:Ensembl P regulation of heart rate by chemical signal
GO:0051591 IEP:RGD P response to cAMP
GO:0042493 IEP:RGD P response to drug
GO:0070542 IEP:RGD P response to fatty acid
GO:0032094 IEP:RGD P response to food
GO:0033762 IEP:RGD P response to glucagon
GO:0009749 IEA:Ensembl P response to glucose
GO:0032868 TAS:BHF-UCL P response to insulin
GO:0033993 IEP:RGD P response to lipid
GO:0014070 IEP:RGD P response to organic cyclic compound
GO:0043434 IEP:RGD P response to peptide hormone
GO:0032570 IEP:RGD P response to progesterone
GO:0032526 IEP:RGD P response to retinoic acid
GO:0006351 IEA:UniProtKB-KW P transcription, DNA-templated

KEGG Pathway Links

KEGG Pathway ID Description
ko04152 AMPK signaling pathway
rno04152 AMPK signaling pathway
ko04910 Insulin signaling pathway
rno04910 Insulin signaling pathway
ko04932 Non-alcoholic fatty liver disease (NAFLD)
rno04932 Non-alcoholic fatty liver disease (NAFLD)

REACTOME Pathway Links

REACTOME Pathway ID Description
5954496 Activation of gene expression by SREBF (SREBP)
5954223 Circadian Clock
5953533 Developmental Biology
5953288 Fatty acid, triacylglycerol, and ketone body metabolism
5953250 Metabolism
5953289 Metabolism of lipids and lipoproteins
5954333 PPARA activates gene expression
5954225 RORA activates circadian gene expression
5954497 Regulation of cholesterol biosynthesis by SREBP (SREBF)
5954222 Regulation of lipid metabolism by Peroxisome proliferator-activated receptor alpha (PPARalpha)
5954166 Transcriptional regulation of white adipocyte differentiation

Domain Information

InterPro Annotations

Accession Description
IPR011598 Myc-type, basic helix-loop-helix (bHLH) domain

UniProt Annotations

Entry Information

Gene Name
sterol regulatory element binding transcription factor 1
Protein Entry
SRBP1_RAT
UniProt ID
P56720
Species
Rat

Comments

Comment Type Description
Alternative Products Event=Alternative splicing; Named isoforms=2; Name=SREBP-1A; IsoId=P56720-2; Sequence=Displayed; Name=SREBP-1C; Synonyms=ADD1; IsoId=P56720-1; Sequence=VSP_030860;
Function Transcriptional activator required for lipid homeostasis. Regulates transcription of the LDL receptor gene as well as the fatty acid and to a lesser degree the cholesterol synthesis pathway (By similarity). Binds to the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3'). Has dual sequence specificity binding to both an E-box motif (5'-ATCACGTGA-3') and to SRE-1 (5'-ATCACCCCAC-3')
Ptm At low cholesterol the SCAP/SREBP complex is recruited into COPII vesicles for export from the ER. In the Golgi complex SREBPs are cleaved sequentially by site-1 and site-2 protease. The first cleavage by site-1 protease occurs within the luminal loop, the second cleavage by site-2 protease occurs within the first transmembrane domain and releases the transcription factor from the Golgi membrane. Apoptosis triggers cleavage by the cysteine proteases caspase-3 and caspase-7 (By similarity)
Ptm Phosphorylated by AMPK, leading to suppress protein processing and nuclear translocation, and repress target gene expression. Phosphorylation at Ser-395 by SIK1 represses activity possibly by inhibiting DNA-binding (By similarity)
Sequence Caution Sequence=L16995; Type=Frameshift; Positions=902, 939, 956, 972, 1016; Evidence= ;
Similarity Belongs to the SREBP family
Similarity Contains 1 bHLH (basic helix-loop-helix) domain
Subcellular Location Endoplasmic reticulum membrane; Multi-pass membrane protein. Golgi apparatus membrane; Multi-pass membrane protein. Cytoplasmic vesicle, COPII-coated vesicle membrane; Multi-pass membrane protein. Note=Moves from the endoplasmic reticulum to the Golgi in the absence of sterols.
Subcellular Location Processed sterol regulatory element-binding protein 1: Nucleus.
Subunit Forms a tight complex with SCAP in the ER membrane. Efficient DNA binding of the soluble transcription factor fragment requires dimerization with another bHLH protein. Interacts with LMNA.
Tissue Specificity Expressed predominantly in brown adipose tissue.

Identical and Related Proteins

Unique RefSeq proteins for LMP012737 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
453040303 RefSeq NP_001263636 1134 sterol regulatory element-binding protein 1 isoform 1 precursor
453040314 RefSeq NP_001263637 1110 sterol regulatory element-binding protein 1 isoform 2

Identical Sequences to LMP012737 proteins

Reference Database Accession Length Protein Name
GI:453040314 GenBank EDM04632.1 1110 sterol regulatory element binding factor 1, isoform CRA_a [Rattus norvegicus]
GI:453040303 GenBank EDM04633.1 1134 sterol regulatory element binding factor 1, isoform CRA_b [Rattus norvegicus]
GI:453040303 SwissProt P56720.3 1134 RecName: Full=Sterol regulatory element-binding protein 1; Short=SREBP-1; AltName: Full=Adipocyte determination- and differentiation-dependent factor 1; Short=ADD1; AltName: Full=Sterol regulatory element-binding transcription factor 1; Contains: RecName: Full=Processed sterol regulatory element-binding protein 1 [Rattus norvegicus]

Related Sequences to LMP012737 proteins

Reference Database Accession Length Protein Name
GI:453040314 GenBank EDM04632.1 1110 sterol regulatory element binding factor 1, isoform CRA_a [Rattus norvegicus]
GI:453040303 GenBank EDM04633.1 1134 sterol regulatory element binding factor 1, isoform CRA_b [Rattus norvegicus]
GI:453040314 RefSeq NP_001263636.1 1134 sterol regulatory element-binding protein 1 isoform 1 precursor [Rattus norvegicus]
GI:453040303 RefSeq NP_001263637.1 1110 sterol regulatory element-binding protein 1 isoform 2 [Rattus norvegicus]
GI:453040303 SwissProt P56720.3 1134 RecName: Full=Sterol regulatory element-binding protein 1; Short=SREBP-1; AltName: Full=Adipocyte determination- and differentiation-dependent factor 1; Short=ADD1; AltName: Full=Sterol regulatory element-binding transcription factor 1; Contains: RecName: Full=Processed sterol regulatory element-binding protein 1 [Rattus norvegicus]
GI:453040314 SwissProt P56720.3 1134 RecName: Full=Sterol regulatory element-binding protein 1; Short=SREBP-1; AltName: Full=Adipocyte determination- and differentiation-dependent factor 1; Short=ADD1; AltName: Full=Sterol regulatory element-binding transcription factor 1; Contains: RecName: Full=Processed sterol regulatory element-binding protein 1 [Rattus norvegicus]