Gene/Proteome Database (LMPD)

LMPD ID
LMP012754
Gene ID
80848
Species
Rattus norvegicus (Rat)
Gene Name
cubilin (intrinsic factor-cobalamin receptor)
Gene Symbol
Cubn
Synonyms
IFCR; MGA1; GP280;
Alternate Names
cubilin; 460 kDa receptor; glycoprotein 280; intrinsic factor-cobalamin receptor; intrinsic factor-vitamin B12 receptor;
Chromosome
17
Map Location
17q12.3
Summary
receptor for intrinsic factor (IF)-cobalamin; involved in vitamin B12 uptake (cyanocobalamin) [RGD, Feb 2006]
Orthologs
View orthologs and multiple alignments for Cubn

Proteins

cubilin precursor
Refseq ID NP_445784
Protein GI 16758040
UniProt ID O70244
mRNA ID NM_053332
Length 3623
MSSQFLWGFVTLLMIAELDGKTGKPEQRGQKRIADLHQPRMTTEEGNLVFLTSSTQNIEFRTGSLGKIKLNDEDLGECLHQIQRNKDDIIDLRKNTTGLPQNILSQVHQLNSKLVDLERDFQNLQQNVERKVCSSNPCLNGGTCVNLHDSFVCICPSQWKGLFCSEDVNECVVYSGTPFGCQSGSTCVNTVGSFRCDCTPDTYGPQCASKYNDCEQGSKQLCKHGICEDLQRVHHGQPNFHCICDAGWTTPPNGISCTEDKDECSLQPSPCSEHAQCFNTQGSFYCGACPKGWQGNGYECQDINECEINNGGCSQAPLVPCLNTPGSFSCGNCPAGFSGDGRVCTPVDICSIHNGGCHPEATCSSSPVLGSFLPVCTCPPGYTGNGYGSNGCVRLSNICSRHPCVNGQCIETVSSYFCKCDSGWSGQNCTENINDCSSNPCLNGGTCIDGINGFTCDCTSSWTGYYCQTPQAACGGILSGTQGTFAYHSPNDTYIHNVNCFWIVRTDEEKVLHVTFTFFDLESASNCPREYLQIHDGDSSADFPLGRYCGSRPPQGIHSSANALYFHLYSEYIRSGRGFTARWEAKLPECGGILTDNYGSITSPGYPGNYPPGRDCVWQVLVNPNSLITFTFGTLSLESHNDCSKDYLEIRDGPFHQDPVLGKFCTSLSTPPLKTTGPAARIHFHSDSETSDKGFHITYLTTQSDLDCGGNYTDTDGELLLPPLSGPFSHSRQCVYLITQAQGEQIVINFTHVELESQMGCSHTYIEVGDHDSLLRKICGNETLFPIRSVSNKVWIRLRIDALVQKASFRADYQVACGGMLRGEGFFRSPFYPNAYPGRRTCRWTISQPQRQVVLLNFTDFQIGSSASCDTDYIEIGPSSVLGSPGNEKFCSSNIPSFITSVYNILYVTFVKSSSMENRGFTAKFSSDKLECGEVLTASTGIIESPGHPNVYPRGVNCTWHVVVQRGQLIRLEFSSFYLEFHYNCTNDYLEIYDTAAQTFLGRYCGKSIPPSLTSNSNSIKLIFVSDSALAHEGFSINYEAIDASSVCLYDYTDNFGMLSSPNFPNNYPSNWECIYRITVGLNQQIALHFTDFTLEDYFGSQCVDFVEIRDGGYETSPLVGIYCGSVLPPTIISHSNKLWLKFKSDAALTAKGFSAYWDGSSTGCGGNLTTPTGVLTSPNYPMPYYHSSECYWRLEASHGSPFELEFQDFHLEHHPSCSLDYLAVFDGPTTNSRLIDKLCGDTTPAPIRSNKDVVLLKLRTDAGQQGRGFEINFRQRCDNVVIVNKTSGILESINYPNPYDKNQRCNWTIQATTGNTVNYTFLGFDVESYMNCSTDYVELYDGPQWMGRYCGNNMPPPGATTGSQLHVLFHTDGINSGEKGFKMQWFTHGCGGEMSGTAGSFSSPGYPNSYPHNKECIWNIRVAPGSSIQLTIHDFDVEYHTSCNYDSLEIYAGLDFNSPRIAQLCSQSPSANPMQVSSTGNELAIRFKTDSTLNGRGFNASWRAVPGGCGGIIQLSRGEIHSPNYPNNYRANTECSWIIQVERHHRVLLNITDFDLEAPDSCLRLMDGSSSTNARVASVCGRQQPPNSIIASGNSLFVRFRSGSSSQNRGFRAEFREECGGRIMTDSSDTIFSPLYPHNYLHNQNCSWIIEAQPPFNHITLSFTHFQLQNSTDCTRDFVEILDGNDYDAPVQGRYCGFSLPHPIISFGNALTVRFVTDSTRSFEGFRAIYSASTSSCGGSFYTLDGIFNSPDYPADYHPNAECVWNIASSPGNRLQLSFLSFNLENSLNCNKDFVEIREGNATGHLIGRYCGNSLPGNYSSAEGHSLWVRFVSDGSGTGMGFQARFKNIFGNNNIVGTHGKIASPFWPGKYPYNSNYKWVVNVDAYHIIHGRILEMDIEPTTNCFYDSLKIYDGFDTHSRLIGTYCGTQTESFSSSRNSLTFQFSSDSSVSGRGFLLEWFAVDVSDSTPPTIAPGACGGFMVTGDTPVHIFSPGWPREYANGADCIWIIYAPDSTVELNILSLDIEPQQSCNYDKLIVKDGDSDLSPELAVLCGVSPPGPIRSTGEYMYIRFTSDTSVAGTGFNASFHKSCGGYLHADRGVITSPKYPDTYLPNLNCSWHVLVQTGLTIAVHFEQPFQIQNRDSFCSQGDYLVLRNGPDNHSPPLGPSGRNGRFCGMYAPSTLFTSGNEMFVQFISDSSNGGQGFKIRYEAKSLACGGTVYIHDADSDGYLTSPNYPANYPQHAECIWILEAPPGRSIQLQFEDQFNIEDTPNCSVSYLELRDGANSNARLVSKLCGHTLPHSWVSSRERIYLKFHTDGGSSYMGFKAKYSIASCGGTVSGDSGVIESIGYPTLPYANNVFCQWFIRGLPGHYLTLSFEDFNLQSSPGCTKDFVEIWENHTSGRVLGRYCGNSTPSSVDTSSNVASVKFVTDGSVTASGFRLQFKSSRQVCGGDLHGPTGTFTSPNYPNPNPHARICEWTITVQEGRRIVLTFTNLRLSTQPSCNSEHLIVFNGIRSNSPLLQKLCSRVNVTNEFKSSGNTMKVVFFTDGSRPYGGFTASYTSTEDAVCGGFLPSVSGGNFSSPGYNGIRDYARNLDCEWTLSNPNRENSSISIYFLELSIESHQDCTFDVLEFRVGDADGPLIEKFCSLSAPTAPLVIPYPQVWIHFVSNERVEYTGFYIEYSFTDCGGIRTGDNGVISSPNYPNLYSAWTHCSWLLKAPEGHTITLTFSDFLLEAHPTCTSDSVTVRNGDSPGSPVIGRYCGQSVPRPIQSGSNQLIVTFNTNNQGQTRGFYATWTTNALGCGGTFHSANGTIKSPHWPQTFPENSRCSWTVITHESKHWEISFDSNFRIPSSDSQCQNSFVKVWEGRLMINKTLLATSCGDVAPSPIVTSGNIFTAVFQSEEMAAQGFSASFISRCGRTFNTSPGDIISPNFPKQYDNNMNCTYLIDADPQSLVILTFVSFHLEDRSAITGTCDHDGLHIIKGRNLSSTPLVTICGSETLRPLTVDGPVLLNFYSDAYTTDFGFKISYRAITCGGIYNESSGILRSPSYSYSNYPNNLYCVYSLHVRSSRVIIIRFNDFDVAPSNLCAHDFLEVFDGPSIGNRSLGKFCGSTRPQTVKSTNSSLTLLFKTDSSQTARGWKIFFRETIGPQQGCGGYLTEDNQSFVSPDSDSNGRYDKGLSCIWYIVAPENKLVKLTFNVFTLEGPSSAGSCVYDYVQIADGASINSYLGGKFCGSRMPAPFISSGNFLTFQFVSDVTVEMRGFNATYTFVDMPCGGTYNATSTPQNASSPHLSNIGRPYSTCTWVIAAPPQQQVQITVWDLQLPSQDCSQSYLELQDSVQTGGNRVTQFCGANYTTLPVFYSSMSTAVVVFKSGVLNRNSQVQFSYQIADCNREYNQTFGNLKSPGWPQNYDNNLDCTIILRAPQNHSISLFFYWFQLEDSRQCMNDFLEVRNGGSSTSPLLDKYCSNLLPNPVFSQSNELYLHFHSDHSVTNNGYEIIWTSSAAGCGGTLLGDEGIFTNPGFPDSYPNNTHCEWTIVAPSGRPVSVGFPFLSIDSSGGCDQNYLIVFNGPDANSPPFGPLCGINTGIAPFYASSNRVFIRFHAEYTTRLSGFEIMWSS
sig_peptide: 1..20 inference: COORDINATES: ab initio prediction:SignalP:4.0 calculated_mol_wt: 2259 peptide sequence: MSSQFLWGFVTLLMIAELDG mat_peptide: 21..3623 product: cubilin name: peripheral membrane protein calculated_mol_wt: 396749 peptide sequence: KTGKPEQRGQKRIADLHQPRMTTEEGNLVFLTSSTQNIEFRTGSLGKIKLNDEDLGECLHQIQRNKDDIIDLRKNTTGLPQNILSQVHQLNSKLVDLERDFQNLQQNVERKVCSSNPCLNGGTCVNLHDSFVCICPSQWKGLFCSEDVNECVVYSGTPFGCQSGSTCVNTVGSFRCDCTPDTYGPQCASKYNDCEQGSKQLCKHGICEDLQRVHHGQPNFHCICDAGWTTPPNGISCTEDKDECSLQPSPCSEHAQCFNTQGSFYCGACPKGWQGNGYECQDINECEINNGGCSQAPLVPCLNTPGSFSCGNCPAGFSGDGRVCTPVDICSIHNGGCHPEATCSSSPVLGSFLPVCTCPPGYTGNGYGSNGCVRLSNICSRHPCVNGQCIETVSSYFCKCDSGWSGQNCTENINDCSSNPCLNGGTCIDGINGFTCDCTSSWTGYYCQTPQAACGGILSGTQGTFAYHSPNDTYIHNVNCFWIVRTDEEKVLHVTFTFFDLESASNCPREYLQIHDGDSSADFPLGRYCGSRPPQGIHSSANALYFHLYSEYIRSGRGFTARWEAKLPECGGILTDNYGSITSPGYPGNYPPGRDCVWQVLVNPNSLITFTFGTLSLESHNDCSKDYLEIRDGPFHQDPVLGKFCTSLSTPPLKTTGPAARIHFHSDSETSDKGFHITYLTTQSDLDCGGNYTDTDGELLLPPLSGPFSHSRQCVYLITQAQGEQIVINFTHVELESQMGCSHTYIEVGDHDSLLRKICGNETLFPIRSVSNKVWIRLRIDALVQKASFRADYQVACGGMLRGEGFFRSPFYPNAYPGRRTCRWTISQPQRQVVLLNFTDFQIGSSASCDTDYIEIGPSSVLGSPGNEKFCSSNIPSFITSVYNILYVTFVKSSSMENRGFTAKFSSDKLECGEVLTASTGIIESPGHPNVYPRGVNCTWHVVVQRGQLIRLEFSSFYLEFHYNCTNDYLEIYDTAAQTFLGRYCGKSIPPSLTSNSNSIKLIFVSDSALAHEGFSINYEAIDASSVCLYDYTDNFGMLSSPNFPNNYPSNWECIYRITVGLNQQIALHFTDFTLEDYFGSQCVDFVEIRDGGYETSPLVGIYCGSVLPPTIISHSNKLWLKFKSDAALTAKGFSAYWDGSSTGCGGNLTTPTGVLTSPNYPMPYYHSSECYWRLEASHGSPFELEFQDFHLEHHPSCSLDYLAVFDGPTTNSRLIDKLCGDTTPAPIRSNKDVVLLKLRTDAGQQGRGFEINFRQRCDNVVIVNKTSGILESINYPNPYDKNQRCNWTIQATTGNTVNYTFLGFDVESYMNCSTDYVELYDGPQWMGRYCGNNMPPPGATTGSQLHVLFHTDGINSGEKGFKMQWFTHGCGGEMSGTAGSFSSPGYPNSYPHNKECIWNIRVAPGSSIQLTIHDFDVEYHTSCNYDSLEIYAGLDFNSPRIAQLCSQSPSANPMQVSSTGNELAIRFKTDSTLNGRGFNASWRAVPGGCGGIIQLSRGEIHSPNYPNNYRANTECSWIIQVERHHRVLLNITDFDLEAPDSCLRLMDGSSSTNARVASVCGRQQPPNSIIASGNSLFVRFRSGSSSQNRGFRAEFREECGGRIMTDSSDTIFSPLYPHNYLHNQNCSWIIEAQPPFNHITLSFTHFQLQNSTDCTRDFVEILDGNDYDAPVQGRYCGFSLPHPIISFGNALTVRFVTDSTRSFEGFRAIYSASTSSCGGSFYTLDGIFNSPDYPADYHPNAECVWNIASSPGNRLQLSFLSFNLENSLNCNKDFVEIREGNATGHLIGRYCGNSLPGNYSSAEGHSLWVRFVSDGSGTGMGFQARFKNIFGNNNIVGTHGKIASPFWPGKYPYNSNYKWVVNVDAYHIIHGRILEMDIEPTTNCFYDSLKIYDGFDTHSRLIGTYCGTQTESFSSSRNSLTFQFSSDSSVSGRGFLLEWFAVDVSDSTPPTIAPGACGGFMVTGDTPVHIFSPGWPREYANGADCIWIIYAPDSTVELNILSLDIEPQQSCNYDKLIVKDGDSDLSPELAVLCGVSPPGPIRSTGEYMYIRFTSDTSVAGTGFNASFHKSCGGYLHADRGVITSPKYPDTYLPNLNCSWHVLVQTGLTIAVHFEQPFQIQNRDSFCSQGDYLVLRNGPDNHSPPLGPSGRNGRFCGMYAPSTLFTSGNEMFVQFISDSSNGGQGFKIRYEAKSLACGGTVYIHDADSDGYLTSPNYPANYPQHAECIWILEAPPGRSIQLQFEDQFNIEDTPNCSVSYLELRDGANSNARLVSKLCGHTLPHSWVSSRERIYLKFHTDGGSSYMGFKAKYSIASCGGTVSGDSGVIESIGYPTLPYANNVFCQWFIRGLPGHYLTLSFEDFNLQSSPGCTKDFVEIWENHTSGRVLGRYCGNSTPSSVDTSSNVASVKFVTDGSVTASGFRLQFKSSRQVCGGDLHGPTGTFTSPNYPNPNPHARICEWTITVQEGRRIVLTFTNLRLSTQPSCNSEHLIVFNGIRSNSPLLQKLCSRVNVTNEFKSSGNTMKVVFFTDGSRPYGGFTASYTSTEDAVCGGFLPSVSGGNFSSPGYNGIRDYARNLDCEWTLSNPNRENSSISIYFLELSIESHQDCTFDVLEFRVGDADGPLIEKFCSLSAPTAPLVIPYPQVWIHFVSNERVEYTGFYIEYSFTDCGGIRTGDNGVISSPNYPNLYSAWTHCSWLLKAPEGHTITLTFSDFLLEAHPTCTSDSVTVRNGDSPGSPVIGRYCGQSVPRPIQSGSNQLIVTFNTNNQGQTRGFYATWTTNALGCGGTFHSANGTIKSPHWPQTFPENSRCSWTVITHESKHWEISFDSNFRIPSSDSQCQNSFVKVWEGRLMINKTLLATSCGDVAPSPIVTSGNIFTAVFQSEEMAAQGFSASFISRCGRTFNTSPGDIISPNFPKQYDNNMNCTYLIDADPQSLVILTFVSFHLEDRSAITGTCDHDGLHIIKGRNLSSTPLVTICGSETLRPLTVDGPVLLNFYSDAYTTDFGFKISYRAITCGGIYNESSGILRSPSYSYSNYPNNLYCVYSLHVRSSRVIIIRFNDFDVAPSNLCAHDFLEVFDGPSIGNRSLGKFCGSTRPQTVKSTNSSLTLLFKTDSSQTARGWKIFFRETIGPQQGCGGYLTEDNQSFVSPDSDSNGRYDKGLSCIWYIVAPENKLVKLTFNVFTLEGPSSAGSCVYDYVQIADGASINSYLGGKFCGSRMPAPFISSGNFLTFQFVSDVTVEMRGFNATYTFVDMPCGGTYNATSTPQNASSPHLSNIGRPYSTCTWVIAAPPQQQVQITVWDLQLPSQDCSQSYLELQDSVQTGGNRVTQFCGANYTTLPVFYSSMSTAVVVFKSGVLNRNSQVQFSYQIADCNREYNQTFGNLKSPGWPQNYDNNLDCTIILRAPQNHSISLFFYWFQLEDSRQCMNDFLEVRNGGSSTSPLLDKYCSNLLPNPVFSQSNELYLHFHSDHSVTNNGYEIIWTSSAAGCGGTLLGDEGIFTNPGFPDSYPNNTHCEWTIVAPSGRPVSVGFPFLSIDSSGGCDQNYLIVFNGPDANSPPFGPLCGINTGIAPFYASSNRVFIRFHAEYTTRLSGFEIMWSS

Gene Information

Entrez Gene ID
80848
Gene Name
cubilin (intrinsic factor-cobalamin receptor)
Gene Symbol
Cubn
Species
Rattus norvegicus

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0031526 IDA:RGD C brush border membrane
GO:0005905 IDA:UniProt C coated pit
GO:0030135 IDA:UniProt C coated vesicle
GO:0030666 IDA:RGD C endocytic vesicle membrane
GO:0005768 IEA:UniProtKB-KW C endosome
GO:0043202 IDA:RGD C lysosomal lumen
GO:0016020 IDA:RGD C membrane
GO:0043234 IDA:RGD C protein complex
GO:0005509 IEA:InterPro F calcium ion binding
GO:0031419 IEA:UniProtKB-KW F cobalamin binding
GO:0015235 IMP:UniProt F cobalamin transporter activity
GO:0030492 IDA:RGD F hemoglobin binding
GO:0042802 IPI:IntAct F identical protein binding
GO:0004872 IDA:RGD F receptor activity
GO:0008203 IEA:UniProtKB-KW P cholesterol metabolic process
GO:0015889 IMP:UniProt P cobalamin transport
GO:0020028 IMP:RGD P hemoglobin import
GO:0001701 IDA:RGD P in utero embryonic development
GO:0070207 IDA:RGD P protein homotrimerization
GO:0006898 IMP:UniProt P receptor-mediated endocytosis
GO:0007584 IMP:RGD P response to nutrient
GO:0006766 TAS:RGD P vitamin metabolic process

KEGG Pathway Links

KEGG Pathway ID Description
ko04977 Vitamin digestion and absorption
rno04977 Vitamin digestion and absorption

Domain Information

InterPro Annotations

Accession Description
IPR000859 CUB domain
IPR028876 Cubilin
IPR024731 EGF domain, merozoite surface protein 1-like
IPR001881 EGF-like calcium-binding domain
IPR018097 EGF-like calcium-binding, conserved site
IPR013032 EGF-like, conserved site
IPR000152 EGF-type aspartate/asparagine hydroxylation site
IPR000742 Epidermal growth factor-like domain

UniProt Annotations

Entry Information

Gene Name
cubilin (intrinsic factor-cobalamin receptor)
Protein Entry
CUBN_RAT
UniProt ID
O70244
Species
Rat

Comments

Comment Type Description
Developmental Stage Expressed at 6 dpc in primitive endoderm cells, in apical membrane invaginations, in endocytic vesicles, endoplasmic reticulum and Golgi apparatus. At the egg cylinder stage (7-8 dpc), expressed in visceral and parietal endoderm. From the early headfold stage (8.9 dpc), expressed in ectodermal cells lining the proamniotic cavity. At 10 dpc, detected in the newly forming neuroepithelium
Domain The CUB domains 5 to 8 mediate binding to GIF and ALB. CUB domains 1 and 2 mediate interaction with LRP2
Function Cotransporter which plays a role in lipoprotein, vitamin and iron metabolism, by facilitating their uptake. Binds to ALB, MB, Kappa and lambda-light chains, TF, hemoglobin, GC, SCGB1A1, APOA1, high density lipoprotein, and the GIF-cobalamin complex. The binding of all ligands requires calcium. Serves as important transporter in several absorptive epithelia, including intestine, renal proximal tubules and embryonic yolk sac. Interaction with LRP2 mediates its trafficking throughout vesicles and facilitates the uptake of specific ligands like GC, hemoglobin, ALB, TF and SCGB1A1. Interaction with AMN controls its trafficking to the plasma membrane and facilitates endocytosis of ligands. May play an important role in the development of the peri-implantation embryo through internalization of APOA1 and cholesterol. Binds to LGALS3 at the maternal-fetal interface. {ECO:0000269|PubMed:10811843, ECO:0000269|PubMed:11805171, ECO:0000269|PubMed:12724130, ECO:0000269|PubMed:15616221, ECO:0000269|PubMed:9153271, ECO:0000269|PubMed:9478979}.
Interaction Self; NbExp=2; IntAct=EBI-3954161, EBI-3954161;
Ptm N-glycosylated
Ptm The precursor is cleaved by a trans-Golgi proteinase furin. The result is a propeptide cleaved off (By similarity)
Similarity Contains 27 CUB domains. {ECO:0000255|PROSITE- ProRule:PRU00059}.
Similarity Contains 7 EGF-like domains. {ECO:0000255|PROSITE- ProRule:PRU00076}.
Subcellular Location Endosome membrane ; Peripheral membrane protein . Lysosome membrane ; Peripheral membrane protein . Note=Colocalizes with AMN and LRP2 in the endocytotic apparatus of epithelial cells.
Subunit Component of the cubam complex composed of CUBN and AMN (By similarity). The cubam complex can oligomerize and form cubam trimers. Interacts with LRP2 in a dual-receptor complex in a calcium-dependent manner. Found in a complex with PID1/PCLI1, LRP1 and CUBNI (By similarity). Interacts with LRP1 and PID1/PCLI1 (By similarity)
Tissue Specificity Expressed to intestinal, renal and yalk sac apical membranes. In kidney, expressed in the proximal tubule

Identical and Related Proteins

Unique RefSeq proteins for LMP012754 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
16758040 RefSeq NP_445784 3623 cubilin precursor

Identical Sequences to LMP012754 proteins

Reference Database Accession Length Protein Name
GI:16758040 GenBank AAC71661.1 3623 intrinsic factor-B12 receptor precursor [Rattus norvegicus]
GI:16758040 SwissProt O70244.2 3623 RecName: Full=Cubilin; AltName: Full=460 kDa receptor; AltName: Full=Glycoprotein 280; Short=gp280; AltName: Full=Intrinsic factor-cobalamin receptor; AltName: Full=Intrinsic factor-vitamin B12 receptor; Flags: Precursor [Rattus norvegicus]

Related Sequences to LMP012754 proteins

Reference Database Accession Length Protein Name
GI:16758040 GenBank AAC71661.1 3623 intrinsic factor-B12 receptor precursor [Rattus norvegicus]
GI:16758040 GenBank EDL78718.1 3623 cubilin (intrinsic factor-cobalamin receptor), isoform CRA_b [Rattus norvegicus]
GI:16758040 SwissProt O70244.2 3623 RecName: Full=Cubilin; AltName: Full=460 kDa receptor; AltName: Full=Glycoprotein 280; Short=gp280; AltName: Full=Intrinsic factor-cobalamin receptor; AltName: Full=Intrinsic factor-vitamin B12 receptor; Flags: Precursor [Rattus norvegicus]