Gene/Proteome Database (LMPD)

LMPD ID
LMP012758
Gene ID
81639
Species
Rattus norvegicus (Rat)
Gene Name
arachidonate 15-lipoxygenase
Gene Symbol
Alox15
Synonyms
12-LOX; 15-LOX; Alox12; Alox12l;
Alternate Names
arachidonate 15-lipoxygenase; 12/15-LOX; 12/15-lipoxygenase; arachidonate omega-6 lipoxygenase; arachidonate 12-lipoxygenase, leukocyte-type;
Chromosome
10
Map Location
10q24
EC Number
1.13.11.33
Summary
demonstrates 12-lipoxygenase and some 15-lipoxygenase enzyme activity [RGD, Feb 2006]
Orthologs
View orthologs and multiple alignments for Alox15

Proteins

arachidonate 15-lipoxygenase
Refseq ID NP_112272
Protein GI 31542125
UniProt ID Q02759
mRNA ID NM_031010
Length 663
MGVYRIRVSTGDSKYAGSNNEVYLWLVGQHGEASLGKLLRPCRDSEAEFKVDVSGYLGPLLFVRVQKWHYLTDDAWFCNWISVKGPGDQGSEYMFPCYRWVQGRSILSLPEGTGCTVVEDSQGLFRKHREEELEERRSLYRWGNWKDGSILNVAAASISDLPVDQRFREDKRIEFEASQVIGVMDTVVNFPINTVTCWKSLDDFNCVFKSGHTKMAERVRNSWKEDAFFGYQFLNGANPMVLKRSTCLPARLVFPPGMEKLQAQLNKELQKGTLFEADFFLLDGIKANVILCSQQYLAAPLVMLKLMPDGQLLPIAIQLELPKTGSTPPPIFTPSDPPMDWLLAKCWVRSSDLQLHELQAHLLRGHLMAEVFAVATMRCLPSVHPVFKLLVPHLLYTMEINVRARSDLISERGFFDKAMSTGGGGHLDLLKQAGAFLTYCSLCPPDDLAERGLLDIETCFYAKDALRLWQIMNRYVVGMFNLHYKTDKAVQDDYELQSWCREITDIGLQGAQDRGFPTSLQSRAQACYFITMCIFTCTAQHSSVHLGQLDWFYWVPNAPCTMRLPPPTTKEATMEKLMATLPNPNQSTLQINVVWLLGRRQAVMVPLGQHSEEHFPNPEAKAVLKKFREELAALDKEIEIRNKSLDIPYEYLRPSMVENSVAI

Gene Information

Entrez Gene ID
81639
Gene Name
arachidonate 15-lipoxygenase
Gene Symbol
Alox15
Species
Rattus norvegicus

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005829 IDA:UniProtKB C cytosol
GO:0031234 ISS:UniProtKB C extrinsic component of cytoplasmic side of plasma membrane
GO:0005811 ISS:UniProtKB C lipid particle
GO:0016020 ISS:UniProtKB C membrane
GO:0005886 ISS:UniProtKB C plasma membrane
GO:0042383 ISS:UniProtKB C sarcolemma
GO:0004052 IDA:RGD F arachidonate 12-lipoxygenase activity
GO:0050473 IDA:RGD F arachidonate 15-lipoxygenase activity
GO:0051120 IDA:RGD F hepoxilin A3 synthase activity
GO:0047977 IDA:UniProtKB F hepoxilin-epoxide hydrolase activity
GO:0005506 ISS:UniProtKB F iron ion binding
GO:0005546 ISS:UniProtKB F phosphatidylinositol-4,5-bisphosphate binding
GO:0019870 TAS:UniProtKB F potassium channel inhibitor activity
GO:0043277 ISS:UniProtKB P apoptotic cell clearance
GO:0019369 IDA:UniProtKB P arachidonic acid metabolic process
GO:0030282 ISS:UniProtKB P bone mineralization
GO:0071277 ISS:UniProtKB P cellular response to calcium ion
GO:0035963 ISS:UniProtKB P cellular response to interleukin-13
GO:0019395 ISS:UniProtKB P fatty acid oxidation
GO:0051122 IDA:UniProtKB P hepoxilin biosynthetic process
GO:0046456 NAS:UniProtKB P icosanoid biosynthetic process
GO:2001303 ISS:UniProtKB P lipoxin A4 biosynthetic process
GO:0019372 IDA:RGD P lipoxygenase pathway
GO:0002820 ISS:UniProtKB P negative regulation of adaptive immune response
GO:0043066 ISS:UniProtKB P negative regulation of apoptotic process
GO:0045794 TAS:UniProtKB P negative regulation of cell volume
GO:0001503 ISS:UniProtKB P ossification
GO:0006646 ISS:UniProtKB P phosphatidylethanolamine biosynthetic process
GO:0030838 ISS:UniProtKB P positive regulation of actin filament polymerization
GO:0030307 ISS:UniProtKB P positive regulation of cell growth
GO:0008284 ISS:UniProtKB P positive regulation of cell proliferation
GO:0010811 ISS:UniProtKB P positive regulation of cell-substrate adhesion
GO:0070374 ISS:UniProtKB P positive regulation of ERK1 and ERK2 cascade
GO:0034116 IMP:RGD P positive regulation of heterotypic cell-cell adhesion
GO:1901074 ISS:UniProtKB P regulation of engulfment of apoptotic cell
GO:0042391 TAS:UniProtKB P regulation of membrane potential
GO:0035358 ISS:UniProtKB P regulation of peroxisome proliferator activated receptor signaling pathway
GO:0034976 ISS:UniProtKB P response to endoplasmic reticulum stress
GO:0042060 ISS:UniProtKB P wound healing

KEGG Pathway Links

KEGG Pathway ID Description
ko00590 Arachidonic acid metabolism
rno00590 Arachidonic acid metabolism
ko00591 Linoleic acid metabolism
rno00591 Linoleic acid metabolism
rno01100 Metabolic pathways
rno04726 Serotonergic synapse

Domain Information

InterPro Annotations

Accession Description
IPR008976 Lipase/lipooxygenase, PLAT/LH2
IPR000907 Lipoxygenase
IPR020834 Lipoxygenase, conserved site
IPR013819 Lipoxygenase, C-terminal
IPR020833 Lipoxygenase, iron binding site
IPR001885 Lipoxygenase, mammalian
IPR001024 PLAT/LH2 domain

UniProt Annotations

Entry Information

Gene Name
arachidonate 15-lipoxygenase
Protein Entry
LOX15_RAT
UniProt ID
Q02759
Species
Rat

Comments

Comment Type Description
Catalytic Activity Arachidonate + O(2) = (5Z,8Z,10E,14Z)-(12S)- 12-hydroperoxyicosa-5,8,10,14-tetraenoate.
Catalytic Activity Arachidonate + O(2) = (5Z,8Z,11Z,13E)-(15S)- 15-hydroperoxyicosa-5,8,11,13-tetraenoate.
Cofactor Name=Fe cation; Xref=ChEBI:CHEBI:24875; Note=Binds 1 Fe cation per subunit.;
Domain The PLAT domain can bind calcium ions; this promotes association with membranes
Function Non-heme iron-containing dioxygenase that catalyzes the stereo-specific peroxidation of free and esterified polyunsaturated fatty acids generating a spectrum of bioactive lipid mediators. Converts arachidonic acid into 12- hydroperoxyeicosatetraenoic acid/12-HPETE and 15- hydroperoxyeicosatetraenoic acid/15-HPETE. Also converts linoleic acid to 13-hydroperoxyoctadecadienoic acid. May also act on (12S)- hydroperoxyeicosatetraenoic acid/(12S)-HPETE to produce hepoxilin A3. Probably plays an important role in the immune and inflammatory responses. Through the oxygenation of membrane-bound phosphatidylethanolamine in macrophages may favor clearance of apoptotic cells during inflammation by resident macrophages and prevent an autoimmune response associated with the clearance of apoptotic cells by inflammatory monocytes. In parallel, may regulate actin polymerization which is crucial for several biological processes, including macrophage function. May also regulate macrophage function through regulation of the peroxisome proliferator activated receptor signaling pathway. Finally, it is also involved in the cellular response to IL13/interleukin-13. In addition to its role in the immune and inflammatory responses, may play a role in epithelial wound healing in the cornea maybe through production of lipoxin A4. May also play a role in endoplasmic reticulum stress response and the regulation of bone mass. {ECO:0000269|PubMed:15123652, ECO:0000269|PubMed:23382512, ECO:0000269|PubMed:8117750, ECO:0000269|PubMed:8444196}.
Pathway Lipid metabolism; hydroperoxy eicosatetraenoic acid biosynthesis.
Similarity Belongs to the lipoxygenase family
Similarity Contains 1 lipoxygenase domain. {ECO:0000255|PROSITE- ProRule:PRU00726}.
Similarity Contains 1 PLAT domain. {ECO:0000255|PROSITE- ProRule:PRU00152}.
Subcellular Location Cytoplasm, cytosol {ECO:0000269|PubMed:8117750}. Cell membrane ; Peripheral membrane protein . Lipid droplet . Note=Predominantly cytosolic; becomes enriched at membranes upon calcium binding. Translocates from the cytosol to the plasma membrane when stimulated by IL13/interleukin-13 and in macrophages binding apoptotic cells (By similarity)
Subunit Interacts with PEBP1; in response to IL13/interleukin-13, prevents the interaction of PEBP1 with RAF1 to activate the ERK signaling cascade
Tissue Specificity Detected in leukocytes, lung and aorta

Identical and Related Proteins

Unique RefSeq proteins for LMP012758 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
31542125 RefSeq NP_112272 663 arachidonate 15-lipoxygenase

Identical Sequences to LMP012758 proteins

Reference Database Accession Length Protein Name
GI:31542125 GenBank AAB30132.1 663 12-lipoxygenase [Rattus sp.]

Related Sequences to LMP012758 proteins

Reference Database Accession Length Protein Name
GI:31542125 GenBank AAA41532.1 663 12-lipoxygenase [Rattus norvegicus]
GI:31542125 GenBank AAB30132.1 663 12-lipoxygenase [Rattus sp.]
GI:31542125 SwissProt Q02759.3 663 RecName: Full=Arachidonate 15-lipoxygenase; Short=15-LOX; AltName: Full=12/15-lipoxygenase; AltName: Full=Arachidonate 12-lipoxygenase, leukocyte-type; Short=12-LOX; AltName: Full=Arachidonate omega-6 lipoxygenase [Rattus norvegicus]