Gene/Proteome Database (LMPD)

LMPD ID
LMP012759
Gene ID
81647
Species
Rattus norvegicus (Rat)
Gene Name
activating transcription factor 2
Gene Symbol
Atf2
Alternate Names
cyclic AMP-dependent transcription factor ATF-2; cAMP-dependent transcription factor ATF-2; cAMP response element-binding protein CRE-BP1;
Chromosome
3
Map Location
3q23
EC Number
2.3.1.48
Summary
transactivates tyrosine hydroxylase transcription; may be involved in regulation of catecholamine synthesis during neural development [RGD, Feb 2006]
Orthologs
View orthologs and multiple alignments for Atf2

Proteins

cyclic AMP-dependent transcription factor ATF-2
Refseq ID NP_112280
Protein GI 13591926
UniProt ID Q00969
mRNA ID NM_031018
Length 487
MSDDKPFLCTAPGCGQRFTNEDHLAVHKHKHEMTLKFGPARNDSVIVADQTPTPTRFLKNCEEVGLFNELASPFENEFKKASEDDIKKMPLDLSPLATPIIRSKIEEPSVVETTHQDSPLPHPESTTNDEKEIPLAQTAQPTSAIVRPASLQVPNVLLTSSDSSVIIQQAVPSPTSSTVITQAPSSNRPIVPVPGPFPLLLHLPNGQTMPVAIPASITSSNVHVPAAVPLVRPVTMVPSVPGIPGPSSPQPVQSEAKMRLKAALTQQHPPVTNGDTVKGHGSGLVRAQSEESRPQSLQQPATSTTETPASPAHTTPQTQNTSGRRRRAANEDPDEKRRKFLERNRAAASRCRQKRKVWVQSLEKKAEDLSSLNGQLQSEVTLLRNEVAQLKQLLLAHKDCPVTAMQKKSGYHTADKDDSSEDLSVPSSPHTEAIQHSSVSTSNGVSSTSKTEAGATSVLTQMADQSTEPALSQIVMAPSSQAQPSGS

Gene Information

Entrez Gene ID
81647
Gene Name
activating transcription factor 2
Gene Symbol
Atf2
Species
Rattus norvegicus

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005737 ISS:UniProtKB C cytoplasm
GO:0005741 ISS:UniProtKB C mitochondrial outer membrane
GO:0005634 IDA:RGD C nucleus
GO:0035861 ISS:UniProtKB C site of double-strand break
GO:0001077 IEA:Ensembl F RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription
GO:0000980 IEA:Ensembl F RNA polymerase II distal enhancer sequence-specific DNA binding
GO:0035497 IDA:RGD F cAMP response element binding
GO:0008140 IEA:Ensembl F cAMP response element binding protein binding
GO:0003682 IEA:Ensembl F chromatin binding
GO:0004402 ISS:UniProtKB F histone acetyltransferase activity
GO:0046872 IEA:UniProtKB-KW F metal ion binding
GO:0046982 IPI:RGD F protein heterodimerization activity
GO:0043565 IDA:RGD F sequence-specific DNA binding
GO:0060612 IEA:Ensembl P adipose tissue development
GO:0006974 ISS:UniProtKB P cellular response to DNA damage stimulus
GO:0045444 IEA:Ensembl P fat cell differentiation
GO:0016573 ISS:GOC P histone acetylation
GO:0031573 ISS:UniProtKB P intra-S DNA damage checkpoint
GO:0003151 IEA:Ensembl P outflow tract morphogenesis
GO:1902110 ISS:UniProtKB P positive regulation of mitochondrial membrane permeability involved in apoptotic process
GO:0051091 ISS:UniProtKB P positive regulation of sequence-specific DNA binding transcription factor activity
GO:0032915 IEA:Ensembl P positive regulation of transforming growth factor beta2 production
GO:0006355 ISS:UniProtKB P regulation of transcription, DNA-templated
GO:0006970 ISS:UniProtKB P response to osmotic stress

KEGG Pathway Links

KEGG Pathway ID Description
ko04261 Adrenergic signaling in cardiomyocytes
rno04261 Adrenergic signaling in cardiomyocytes
ko05034 Alcoholism
rno05034 Alcoholism
ko05031 Amphetamine addiction
rno05031 Amphetamine addiction
ko05030 Cocaine addiction
rno05030 Cocaine addiction
ko04728 Dopaminergic synapse
rno04728 Dopaminergic synapse
ko05169 Epstein-Barr virus infection
rno05169 Epstein-Barr virus infection
ko04915 Estrogen signaling pathway
rno04915 Estrogen signaling pathway
ko05166 HTLV-I infection
rno05166 HTLV-I infection
rno05161 Hepatitis B
ko05164 Influenza A
rno05164 Influenza A
rno04911 Insulin secretion
ko04010 MAPK signaling pathway
rno04010 MAPK signaling pathway
ko04151 PI3K-Akt signaling pathway
rno04151 PI3K-Akt signaling pathway
ko04668 TNF signaling pathway
rno04668 TNF signaling pathway
ko04918 Thyroid hormone synthesis
rno04918 Thyroid hormone synthesis
ko05203 Viral carcinogenesis
rno05203 Viral carcinogenesis
ko04022 cGMP-PKG signaling pathway
rno04022 cGMP-PKG signaling pathway

REACTOME Pathway Links

REACTOME Pathway ID Description
5953551 Activated TLR4 signalling
5953785 Activation of the AP-1 family of transcription factors
5954609 Chromatin modifying enzymes
5954610 Chromatin organization
5954617 HATs acetylate histones
5953410 Immune System
5953409 Innate Immune System
5953549 MAP kinase activation in TLR cascade
5953786 MAPK targets/ Nuclear events mediated by MAP kinases
5953565 MyD88 cascade initiated on plasma membrane
5953562 MyD88 dependent cascade initiated on endosome
5953559 MyD88-independent cascade
5953550 MyD88:Mal cascade initiated on plasma membrane
5953561 TRAF6 mediated induction of NFkB and MAP kinases upon TLR7/8 or 9 activation
5953558 TRIF-mediated TLR3/TLR4 signaling
5953566 Toll Like Receptor 10 (TLR10) Cascade
5953555 Toll Like Receptor 2 (TLR2) Cascade
5953560 Toll Like Receptor 3 (TLR3) Cascade
5953552 Toll Like Receptor 4 (TLR4) Cascade
5953567 Toll Like Receptor 5 (TLR5) Cascade
5953564 Toll Like Receptor 7/8 (TLR7/8) Cascade
5953563 Toll Like Receptor 9 (TLR9) Cascade
5953554 Toll Like Receptor TLR1:TLR2 Cascade
5953556 Toll Like Receptor TLR6:TLR2 Cascade
5953553 Toll-Like Receptors Cascades

Domain Information

InterPro Annotations

Accession Description
IPR004827 Basic-leucine zipper domain
IPR016378 TF_cAMP-dep
IPR013087 Zinc finger C2H2-type/integrase DNA-binding domain
IPR007087 Zinc finger, C2H2
IPR015880 Zinc finger, C2H2-like

UniProt Annotations

Entry Information

Gene Name
activating transcription factor 2
Protein Entry
ATF2_RAT
UniProt ID
Q00969
Species
Rat

Comments

Comment Type Description
Alternative Products Event=Alternative splicing; Named isoforms=2; Name=1; IsoId=Q00969-1; Sequence=Displayed; Name=2; IsoId=Q00969-2; Sequence=VSP_000591;
Catalytic Activity Acetyl-CoA + [histone] = CoA + acetyl- [histone]
Function Transcriptional activator which regulates the transcription of various genes, including those involved in anti- apoptosis, cell growth, and DNA damage response. Dependent on its binding partner, binds to CRE (cAMP response element) consensus sequences (5'-TGACGTCA-3') or to AP-1 (activator protein 1) consensus sequences (5'-TGACTCA-3'). In the nucleus, contributes to global transcription and the DNA damage response, in addition to specific transcriptional activities that are related to cell development, proliferation and death. In the cytoplasm, interacts with and perturbs HK1- and VDAC1-containing complexes at the mitochondrial outer membrane, thereby impairing mitochondrial membrane potential, inducing mitochondrial leakage and promoting cell death. The phosphorylated form (mediated by ATM) plays a role in the DNA damage response and is involved in the ionizing radiation (IR)-induced S phase checkpoint control and in the recruitment of the MRN complex into the IR-induced foci (IRIF). Exhibits histone acetyltransferase (HAT) activity which specifically acetylates histones H2B and H4 in vitro. In concert with CUL3 and RBX1, promotes the degradation of KAT5 thereby attenuating its ability to acetylate and activate ATM. Can elicit oncogenic or tumor suppressor activities depending on the tissue or cell type (By similarity)
Ptm Phosphorylation of Thr-51 by MAPK14 and MAPK11, and at Thr-53 by MAPK1/ERK2, MAPK3/ERK1, MAPK11, MAPK12 and MAPK14 in response to external stimulus like insulin causes increased transcriptional activity (By similarity). Phosphorylated by PLK3 following hyperosmotic stress. Also phosphorylated and activated by JNK and CaMK4 (By similarity). ATM-mediated phosphorylation at Ser-472 and Ser-480 stimulates its function in DNA damage response. Phosphorylation at Ser-44, Thr-55 and Ser-103 activates its transcriptional activity. Phosphorylation at Thr-51 or Thr-53 enhances its histone acetyltransferase (HAT) activity (By similarity)
Similarity Belongs to the bZIP family. ATF subfamily
Similarity Contains 1 C2H2-type zinc finger
Similarity Contains 1 bZIP (basic-leucine zipper) domain
Subcellular Location Nucleus. Cytoplasm . Mitochondrion outer membrane . Note=Shuttles between the cytoplasm and the nucleus and heterodimerization with JUN is essential for the nuclear localization. Localization to the cytoplasm is observed under conditions of cellular stress and in disease states. Localizes at the mitochondrial outer membrane in response to genotoxic stress. Phosphorylation at Thr-34 is required for its nuclear localization and negatively regulates its mitochondrial localization. Colocalizes with the MRN complex in the IR-induced foci (IRIF) (By similarity)
Subunit Binds DNA as a dimer and can form a homodimer in the absence of DNA. Can form a heterodimer with JUN. Heterodimerization is essential for its transcriptional activity. Interacts with SMAD3 and SMAD4. Binds through its N-terminal region to UTF1 which acts as a coactivator of ATF2 transcriptional activity (By similarity). Interacts with the HK1/VDAC1 complex. Interacts with NBN, MRE11A, XPO1, KAT5 and CUL3 (By similarity)

Identical and Related Proteins

Unique RefSeq proteins for LMP012759 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
13591926 RefSeq NP_112280 487 cyclic AMP-dependent transcription factor ATF-2

Identical Sequences to LMP012759 proteins

Reference Database Accession Length Protein Name
GI:13591926 GenBank AAA93263.1 487 cAMP response element binding protein 1 [Rattus norvegicus]
GI:13591926 GenBank EDL79159.1 487 rCG26528, isoform CRA_a [Rattus norvegicus]
GI:13591926 GenBank EDL79160.1 487 rCG26528, isoform CRA_a [Rattus norvegicus]
GI:13591926 SwissProt Q00969.2 487 RecName: Full=Cyclic AMP-dependent transcription factor ATF-2; Short=cAMP-dependent transcription factor ATF-2; AltName: Full=Activating transcription factor 2; AltName: Full=cAMP response element-binding protein CRE-BP1 [Rattus norvegicus]

Related Sequences to LMP012759 proteins

Reference Database Accession Length Protein Name
GI:13591926 GenBank EDL79159.1 487 rCG26528, isoform CRA_a [Rattus norvegicus]
GI:13591926 GenBank EDL79160.1 487 rCG26528, isoform CRA_a [Rattus norvegicus]
GI:13591926 SwissProt Q00969.2 487 RecName: Full=Cyclic AMP-dependent transcription factor ATF-2; Short=cAMP-dependent transcription factor ATF-2; AltName: Full=Activating transcription factor 2; AltName: Full=cAMP response element-binding protein CRE-BP1 [Rattus norvegicus]