Gene/Proteome Database (LMPD)

LMPD ID
LMP012760
Gene ID
81654
Species
Rattus norvegicus (Rat)
Gene Name
dihydrolipoamide S-acetyltransferase
Gene Symbol
Dlat
Alternate Names
dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial; E2; PBC; PDCE2; PDC-E2; pyruvate dehydrogenase complex component E2; 70 kDa mitochondrial autoantigen of primary biliary cirrhosis; dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; dihydrolipoamide S-acetyltransferase (E2 component of pyruvate dehydrogenase complex);
Chromosome
8
Map Location
8q23
EC Number
2.3.1.12
Summary
putative dihydrolipoamide acetyltransferase [RGD, Feb 2006]
Orthologs
View orthologs and multiple alignments for Dlat

Proteins

dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial
Refseq ID NP_112287
Protein GI 78365255
UniProt ID P08461
mRNA ID NM_031025
Length 632
MWRVCARRVQSAVPRAGFRARWATLKGPRTGPAAVRCGSGIPSYGVRSLCGWSYGSATVPRNRILQQLLGSPSRRSYSLPPHQKVPLPSLSPTMQAGTIARWEKKEGEKISEGDLIAEVETDKATVGFESLEECYMAKILVPEGTRDVPVGSIICITVEKPQDIEAFKNYTLDSATAATQAAPAPAAAPAAAPAAPSASAPGSSYPVHMQIVLPALSPTMTMGTVQRWEKKVGEKLSEGDLLAEIETDKATIGFEVQEEGYLAKILVPEGTRDVPLGTPLCIIVEKQEDIAAFADYRPTEVTSLKPQAPPPVPPPVAAVPPIPQPLAPTPSAAPAGPKGRVFVSPLAKKLAAEKGIDLTQVKGTGPEGRIIKKDIDSFVPTKAAPAAAAAAPPGPRVAPTPAGVFIDIPISNIRRVIAQRLMQSKQTIPHYYLSVDVNMGEVLLVRKELNKMLEGKGKISVNDFIIKASALACLKVPEANSSWMDTVIRQNHVVDVSVAVSTPAGLITPIVFNAHIKGLETIASDVVSLASKAREGKLQPHEFQGGTFTISNLGMFGIKNFSAIINPPQACILAIGASEDKLIPADNEKGFDVASVMSVTLSCDHRVVDGAVGAQWLAEFKKYLEKPVTMLL
transit_peptide: 1..77 experiment: experimental evidence, no additional details recorded note: Mitochondrion; propagated from UniProtKB/Swiss-Prot (P08461.3) calculated_mol_wt: 8450 peptide sequence: MWRVCARRVQSAVPRAGFRARWATLKGPRTGPAAVRCGSGIPSYGVRSLCGWSYGSATVPRNRILQQLLGSPSRRSY mat_peptide: 78..632 product: Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial experiment: experimental evidence, no additional details recorded note: propagated from UniProtKB/Swiss-Prot (P08461.3) calculated_mol_wt: 58734 peptide sequence: SLPPHQKVPLPSLSPTMQAGTIARWEKKEGEKISEGDLIAEVETDKATVGFESLEECYMAKILVPEGTRDVPVGSIICITVEKPQDIEAFKNYTLDSATAATQAAPAPAAAPAAAPAAPSASAPGSSYPVHMQIVLPALSPTMTMGTVQRWEKKVGEKLSEGDLLAEIETDKATIGFEVQEEGYLAKILVPEGTRDVPLGTPLCIIVEKQEDIAAFADYRPTEVTSLKPQAPPPVPPPVAAVPPIPQPLAPTPSAAPAGPKGRVFVSPLAKKLAAEKGIDLTQVKGTGPEGRIIKKDIDSFVPTKAAPAAAAAAPPGPRVAPTPAGVFIDIPISNIRRVIAQRLMQSKQTIPHYYLSVDVNMGEVLLVRKELNKMLEGKGKISVNDFIIKASALACLKVPEANSSWMDTVIRQNHVVDVSVAVSTPAGLITPIVFNAHIKGLETIASDVVSLASKAREGKLQPHEFQGGTFTISNLGMFGIKNFSAIINPPQACILAIGASEDKLIPADNEKGFDVASVMSVTLSCDHRVVDGAVGAQWLAEFKKYLEKPVTMLL

Gene Information

Entrez Gene ID
81654
Gene Name
dihydrolipoamide S-acetyltransferase
Gene Symbol
Dlat
Species
Rattus norvegicus

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005739 IDA:RGD C mitochondrion
GO:0045254 IDA:RGD C pyruvate dehydrogenase complex
GO:0004742 IDA:RGD F dihydrolipoyllysine-residue acetyltransferase activity
GO:0000166 TAS:RGD F nucleotide binding
GO:0006086 IDA:RGD P acetyl-CoA biosynthetic process from pyruvate
GO:0006006 IEA:UniProtKB-KW P glucose metabolic process
GO:0030431 IEP:RGD P sleep
GO:0006099 IEA:UniProtKB-KW P tricarboxylic acid cycle

KEGG Pathway Links

KEGG Pathway ID Description
ko01200 Carbon metabolism
rno01200 Carbon metabolism
ko00020 Citrate cycle (TCA cycle)
rno00020 Citrate cycle (TCA cycle)
ko00010 Glycolysis / Gluconeogenesis
rno00010 Glycolysis / Gluconeogenesis
rno01100 Metabolic pathways
ko00620 Pyruvate metabolism
rno00620 Pyruvate metabolism
M00307 Pyruvate oxidation, pyruvate => acetyl-CoA

REACTOME Pathway Links

REACTOME Pathway ID Description
5953250 Metabolism
5953268 Pyruvate metabolism
5953269 Pyruvate metabolism and Citric Acid (TCA) cycle
5954047 Regulation of pyruvate dehydrogenase (PDH) complex
5953270 The citric acid (TCA) cycle and respiratory electron transport

Domain Information

InterPro Annotations

Accession Description
IPR003016 2-oxo acid dehydrogenase, lipoyl-binding site
IPR001078 2-oxoacid dehydrogenase acyltransferase, catalytic domain
IPR000089 Biotin/lipoyl attachment
IPR023213 Chloramphenicol acetyltransferase-like domain
IPR006257 Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex
IPR004167 E3-binding domain
IPR011053 Single hybrid motif

UniProt Annotations

Entry Information

Gene Name
dihydrolipoamide S-acetyltransferase
Protein Entry
ODP2_RAT
UniProt ID
P08461
Species
Rat

Comments

Comment Type Description
Catalytic Activity Acetyl-CoA + enzyme N(6)-(dihydrolipoyl)lysine = CoA + enzyme N(6)-(S-acetyldihydrolipoyl)lysine.
Cofactor Name=(R)-lipoate; Xref=ChEBI:CHEBI:83088; Note=Binds 1 lipoyl cofactor covalently.;
Disease Note=Primary biliary cirrhosis (PBC) is an autoimmune disease characterized by inflammatory obliteration of intra- hepatic bile duct, leading to liver cell damage and cirrhosis. The E2 component of pyruvate dehydrogenase complex is the autoantigen for PBC.
Function The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links the glycolytic pathway to the tricarboxylic cycle.
Similarity Belongs to the 2-oxoacid dehydrogenase family
Similarity Contains 2 lipoyl-binding domains
Subcellular Location Mitochondrion matrix.
Subunit Part of the multimeric pyruvate dehydrogenase complex that contains multiple copies of pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (DLAT, E2) and lipoamide dehydrogenase (DLD, E3). These subunits are bound to an inner core composed of about 48 DLAT and 12 PDHX molecules. Interacts with PDK2 and PDK3 (By similarity)
Tissue Specificity Expressed in flagella of epididymal sperm

Identical and Related Proteins

Unique RefSeq proteins for LMP012760 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
78365255 RefSeq NP_112287 632 dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial

Identical Sequences to LMP012760 proteins

Reference Database Accession Length Protein Name
GI:78365255 GenBank AAI07441.1 632 Dihydrolipoamide S-acetyltransferase [Rattus norvegicus]
GI:78365255 GenBank EDL95470.1 632 dihydrolipoamide S-acetyltransferase (E2 component of pyruvate dehydrogenase complex) [Rattus norvegicus]
GI:78365255 SwissProt P08461.3 632 RecName: Full=Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial; AltName: Full=70 kDa mitochondrial autoantigen of primary biliary cirrhosis; Short=PBC; AltName: Full=Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; AltName: Full=Pyruvate dehydrogenase complex component E2; Short=PDC-E2; Short=PDCE2; Flags: Precursor [Rattus norvegicus]

Related Sequences to LMP012760 proteins

Reference Database Accession Length Protein Name
GI:78365255 GenBank AAI07441.1 632 Dihydrolipoamide S-acetyltransferase [Rattus norvegicus]
GI:78365255 GenBank EDL95470.1 632 dihydrolipoamide S-acetyltransferase (E2 component of pyruvate dehydrogenase complex) [Rattus norvegicus]
GI:78365255 SwissProt P08461.3 632 RecName: Full=Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial; AltName: Full=70 kDa mitochondrial autoantigen of primary biliary cirrhosis; Short=PBC; AltName: Full=Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; AltName: Full=Pyruvate dehydrogenase complex component E2; Short=PDC-E2; Short=PDCE2; Flags: Precursor [Rattus norvegicus]